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Citations for Millipore Sigma :
101 - 150 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The PCR conditions were set up to amplify only the short 670 bp product using REDExtract-N-Amp PCR ReadyMix (Sigma). PCR conditions ...
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bioRxiv - Genomics 2019Quote: ... was used along with 0.36 μl of 50 μM custom-designed SYNE2 short and long isoform primer and 0.5 μl of 10 μM probe set (Table 1, obtained from Sigma-Aldrich) and 1 μl of the primer limited cyclophilin A (PPIA ...
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bioRxiv - Immunology 2022Quote: ... qPCR was performed using PerfeCTa SYBR Green FastMix Reaction Mix (Green Fastmix, ROX™) (QuantaBio) and KiCqStart primer sets (Sigma). ELISAs were performed as per the manufacturer’s instructions (R&D Systems).
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bioRxiv - Neuroscience 2021Quote: ... Overlapping PCR primers encoding the gRNA sequences were ordered from Sigma-Aldrich and Eurofins Genomics ...
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bioRxiv - Genomics 2022Quote: ... PCR amplifications were set up in 25μl total volume reaction mixture containing water (Sigma-Aldrich) (18.4μl) ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from DUSP16 and KSR1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3 primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Genetics 2021Quote: ... The induction of the top 10 HITS from IFIH1 were validated in these samples via qPCR (PCR primer sequences used are shown in Additional file 1: Table S3; primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-PCR products were separated by gel electrophoresis in 1% agarose (Sigma). Gene-Ruler 100 bp DNA ladder (ThermoScientific ...
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bioRxiv - Molecular Biology 2021Quote: AC16 human cardiomyocytes were purchased from EMD Millipore (SSC #109) and cultured in Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Developmental Biology 2020Quote: ... 2x SSC and 10% (w/v) dextran sulfate (Sigma, D8906) in a humid chamber at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... respectively: 75% probe wash buffer + 25% 5x SSC (Sigma Aldrich), 50% probe was buffer + 50% 5x SSC ...
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bioRxiv - Biophysics 2024Quote: ... Cells were then washed with 2x SSC (Sigma-Aldrich, #335266) and incubated in a buffer of 2x SSC ...
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bioRxiv - Systems Biology 2023Quote: ... slides have been washed with 0.1X SSC buffer (Sigma S6639), and ddH2O ...
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bioRxiv - Microbiology 2021Quote: Colonies that produced bands of the expected size with both primer sets were inoculated into 1 ml of LB containing 1% arabinose (Sigma-Aldrich) for 1 hour at 30°C to induce I-Sce expression to linearize the inserted KanSacB cassette ...
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bioRxiv - Immunology 2022Quote: ... and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831; EMD Millipore) as described (83) ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were either PCR-amplified with oligonucleotide primers (Sigma-Aldrich and IDT) or ordered as gBlocks from IDT and cloned into Gateway pENTR plasmids ...
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Liver X Receptor activation regulates genes involved in lipid homeostasis in developing chondrocytesbioRxiv - Physiology 2019Quote: ... and all real-time PCR reagents and primers were obtained from Sigma-Aldrich. All organ culture and cell culture media components were obtained from Sigma-Aldrich and Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... with PCR primers with overhangs containing restriction sites for SpeI and NotI (Sigma). pLVX-EF1α-IRES-ZsGreen1 (Cat# 631982 ...
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bioRxiv - Neuroscience 2024Quote: ... Primers for SYBR Green-based PCR of mRNA were obtained from Sigma Aldrich, miRCURY LNA assays for qPCR of miRNA were purchased from Qiagen ...
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bioRxiv - Immunology 2023Quote: ... and transcripts quantified by real-time semi-quantitative PCR using KiCqStart primers (Sigma) and iTaq SYBR green (Accuscience ...
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bioRxiv - Microbiology 2023Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene PA3617 (recA ...
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bioRxiv - Microbiology 2024Quote: ... PCR assay was conducted by using gene specific primers procured from Sigma-Aldrich. The gene rpsU was kept as an endogenous control ...
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bioRxiv - Cancer Biology 2023Quote: miR-6883 mimic was purchased from Sigma-Aldrich (HMI2616). This double-stranded RNA molecule mimics endogenous mature miRNA when introduced into cells.
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with SYBR Green Quantitative RT-qPCR Kit (QR0100; Sigma) or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were rehydrated with 10% formamide in 2X SSC (Sigma Aldrich), twice for 5 minutes at RT ...
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bioRxiv - Systems Biology 2020Quote: ... 2× SSC and 10% (w/v) dextran sulfate (Millipore 3710-OP). The hybridization was performed at 37°C for 24-72 hours in a humid chamber ...
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bioRxiv - Systems Biology 2020Quote: ... 2× SSC and 10% (w/v) dextran sulfate (Millipore 3710-OP) was hybridized at 37°C for 48-96 hours in a humid chamber ...
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bioRxiv - Systems Biology 2021Quote: ... 2× SSC and 10% (w/v) dextran sulfate (Millipore 3710-OP). The hybridization was performed at 37°C for 3 days in a humid chamber ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then covered with 5X SSC (Sigma-Aldrich, S6639-1L) buffer and left overnight until the morning ...
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bioRxiv - Cancer Biology 2022Quote: ... each well was washed with 100 μL 0.1×SSC (Sigma-Aldrich), and added with 75 μL reverse transcription Master Mix (from the Visium Reagent Kit ...
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bioRxiv - Neuroscience 2022Quote: ... the coated slide was washed 5 times with SSC 2X (Sigma) followed by 2 washes with dH2O ...
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bioRxiv - Cancer Biology 2021Quote: ... Metaphase slides in coplin jar containing 2X SSC buffer (Sigma-S6639) were equilibrated at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... SSC (side scatter) and DAPI (4’,6-diamino-2-phenylindole; Sigma). FACS analyses were carried out using BD LSRII flow cytometry (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was washed twice with 2X SSC buffer (Sigma-Aldrich), dried ...
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bioRxiv - Cell Biology 2022Quote: Individual cells were sorted into 96-well plates containing 4μL of lysis buffer and index RT primers (0,2% Triton (Sigma Aldrich), 0,4 U/μL RNaseOUT (Thermofisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: Genotyping for the R1.40 transgene locus was done with the recommended PCR primers using the PCR Ready Mix kit (E3004; Sigma-Aldrich). The sequences of the genotyping primers were as follows ...
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bioRxiv - Biochemistry 2022Quote: ... The sequence was verified by colony PCR using standard primers (Sigma-Aldrich, Taufkirchen, Germany) and Taq polymerase (In house ...
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bioRxiv - Microbiology 2024Quote: ... amplified with the primers P2 and P3 (S2 Table) by KOD PCR (Sigma-Aldrich). Transfected parasites were selected in medium containing mycophenolic acid (25 μg/ml ...
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bioRxiv - Genetics 2023Quote: ... specific primers were designed and PCR was performed using KOD DNA polymerase (Sigma Aldrich). The amplification of DNA fragments was done following manufacturer’s instructions into a Bioer GeneExplorer thermal Cycler ...
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bioRxiv - Cell Biology 2023Quote: ... cDNAs were mixed with gene specific primers and SYBR green PCR MasterMix (Sigma Aldrich) and run on an Applied Biosystems 7900HT Fast Real-Time PCR system.
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bioRxiv - Cancer Biology 2019Quote: ... miR-325 and control miRNA were purchased from Sigma-Aldrich.
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bioRxiv - Molecular Biology 2019Quote: ... Primers are from Sigma (KiCqStartTM Primers).
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bioRxiv - Plant Biology 2022Quote: All primers (Sigma Aldrich UK. Primers) for gene targets (Supplementary Table S1) ...
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bioRxiv - Cell Biology 2021Quote: ... qRT-PCR was performed with Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07; Sigma). Inventoried TaqMan probes for qRT-PCR from Applied Biosystems were used for human TULP3 and GAPDH ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR was carried out using SYBR Green (Jumpstart Taq ReadyMix: Sigma-Aldrich) in 10 μl volumes for 3 biological and 3 technical replicates with 1:10 diluted cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using FastStart Universal SYBR Green Master (Rox) (Sigma, 4913850001) on a Bio-Rad CFX Connect Real-Time PCR detection system ...
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bioRxiv - Immunology 2023Quote: ... IL10 and normalization control GAPDH were examined by RT-qPCR using gene specific primers (Sigma Aldrich, St. Louis, MO, USA) as previously described 11 ...
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bioRxiv - Biochemistry 2021Quote: ... the Msm guaB1 gene was amplified by PCR by primer pair 17/18 and inserted into the PCR-linearized pTriex-4 vector (Novagen, USA) with primers 15/16 by the In-Fusion approach ...
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bioRxiv - Genetics 2019Quote: ... SSCs were treated with culture medium containing colchicine (100 ng/ml; Sigma) for 3 hours ...
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bioRxiv - Genomics 2020Quote: ... Sequences were PCR amplified from zebrafish genomic DNA using the primers in SupplementaryTable16 (Sigma-Aldrich), with the proof-reading iMax ™ II DNA polymerase (INtRON Biotechnology ...