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Citations for Millipore Sigma :
101 - 150 of 3815 citations for Lymphocyte antigen 6H LY6H Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: HEK293 cells were cultured in low-glucose DMEM (Sigma) supplemented with 10% FBS (Capricorn Scientific) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured on poly-D-lysine (Millipore) coated glass coverslips ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... mouse and human brain slices of 20 μm thickness were subjected to antigen retrieval in 10 mM citrate (Sigma-Aldrich, St. Louis, MO, USA) + 0.5% Tween-20 (Merck ...
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bioRxiv - Immunology 2020Quote: ... The evaluation of the effect of reactive oxygen species was done by treating the cells for 6h with 10 mM of N-Acetyl-L-Cysteine (Millipore, 1009005).
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bioRxiv - Neuroscience 2020Quote: ... the nitrocellulose membranes underwent a 1h blocking step in Rockland buffer before 6h incubation with primary antibodies (for all antibody RRIDS, see Key Resources Table): mouse α-KCC2 (1:1000, Millipore), rabbit α-phospho-KCC2-S940 (1:1000 Aviva Systems Biology) ...
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bioRxiv - Cell Biology 2022Quote: Larval brains expressing UAS-mCD8-GFP under grh-Gal4 at 6h ALH were dissected in Shield and Sang M3 insect medium (Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was replaced with medium containing the corresponding treatments and 6h a pulse of BrdU (5-bromo-2-deoxyuridine, 50 µM; Sigma-Aldrich) was given to the neonatal cells ...
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bioRxiv - Microbiology 2019Quote: ... anti-His (1:1000 Sigma), or anti-StrepII (1:3000 IBA ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-His (Sigma Aldrich), rabbit anti-Tubulin polyclonal antibody (Cell Signaling Technology).
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Genetics 2021Quote: ... for antigen retrieval and anti-acetylated tubulin (Sigma) 1/300 in PBS containing 0.5% of normal goat serum and 0.05% Tween20 ...
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bioRxiv - Physiology 2021Quote: ... and pericytes (neuron-glial antigen 2; NG2; Millipore). tdTomato was used as a reporter of ChR2 expression and amplified with an antibody against mCherry ...
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bioRxiv - Bioengineering 2020Quote: ... Flavivirus Group Antigen (Millipore Sigma, MAB10216, 1:200), GABA (Sigma ...
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bioRxiv - Physiology 2022Quote: ... Antigen retrieval was performed with 0.03% protease (Sigma) for 15 min at 37ºC and endogenous peroxide activity was blocked with 3% hydrogen peroxide for 30 mins ...
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bioRxiv - Developmental Biology 2019Quote: ... Antigens were made using a pET28b (+) plasmid (Novagen) for expression of 6XHis tagged proteins ...
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bioRxiv - Cancer Biology 2019Quote: ... incubated with antigen retrieval agent (Reblot strong, Millipore) for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse anti-SV40-T antigens (MABF121, EMD Millipore), and mouse anti-β-actin (A5441 ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.5 antigen retrieval solution (E1161, Sigma-Aldrich) for 20 min in a thermoregulated bath ...
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bioRxiv - Immunology 2023Quote: ... The following antigens were used: ovalbumin (Sigma; A5503), Ara h 2 (ALK Abelló) ...
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bioRxiv - Neuroscience 2024Quote: ... antigen retriever (#C9999, Sigma-Aldrich, Saint Luis, MO). For the detection of disease-associated PrP ...
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bioRxiv - Biochemistry 2020Quote: ... or 2 μM in triplicate for 6h or 18h followed by fixation and immunostaining for the acetyl-histone H3- Lys9 mark (monoclonal Anti-H3K9Ac; Millipore, #07-352). Nine images at 20X magnification were collected and analyzed from each well from a 96-well plate on an automated confocal microscope ...
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bioRxiv - Genomics 2020Quote: ... CD19+ B-lymphocytes and CD14+ monocytes were isolated by negative selection using RosetteSep kits (Sigma). Macrophages were produced from monocytes by culturing in RPMI with 20% fetal calf serum with 50 ng/ml M-CSF for one week as described [53] ...
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bioRxiv - Immunology 2020Quote: ... Lymphocytes were isolated by a density-gradient technique (Ficoll-Histopaque; Sigma, St Louis, MO, USA). CD56+ NK cells ...
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bioRxiv - Immunology 2021Quote: ... more than 1 × 107 lymphocytes were isolated from peripheral blood by Ficoll 1.077 (Sigma Aldrich) separation ...
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bioRxiv - Immunology 2020Quote: ... B lymphocytes were stimulated to undergo class switching with 5 μg/ml LPS (Sigma-Aldrich) and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Lymphocytes were obtained from the resulting cell suspensions using density gradient centrifugation (Percoll, SIGMA-ALDRICH). Cells were recovered at the interface of the 80% Percoll layer and the 40% Percoll layer ...
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bioRxiv - Immunology 2022Quote: ... for the limiting antigen condition and 2 µg/ml (4000 molecules/μm2) for the high antigen condition) and streptavidin (Sigma-Aldrich) were added to the flow chambers with or without 0.5 µg/ml unlabeled histidine-tagged ICAM-1 ...
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bioRxiv - Cell Biology 2020Quote: Wild type Hek293 cells were grown in DMEM (Sigma D6429) supplemented with 10% FCS ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... while HEK293 and A549 cells were cultured in DMEM (Sigma). Both were supplemented with 10% foetal calf serum (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: Hela and HEK293 cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10 % fetal bovine serum (PAN Biotech) ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were cultured in high glucose DMEM (Sigma-Aldrich) containing 10% FBS (HyClone) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cell lines were cultured with MEM medium (Sigma-Aldrich) supplied with FBS (Funakoshi) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were grown in Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 10% fetal bovine serum ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were incubated in culture with 1.5% DMS (Sigma) at room temperature for 7 minutes ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured on poly-D-lysine (Sigma-Aldrich) coated glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: HEK293 derived-nsEVs were resuspended with dextran-FITC (Sigma-Aldrich) or seed saporin (SAP ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 T-REx cells were treated with RocA (Sigma– Aldrich) at a concentration of 0.3 μM or 3 μM and incubated for 30 min before cell lysis ...
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bioRxiv - Molecular Biology 2021Quote: ... and input TRF1 mutants were revealed with anti-His (anti 6-His Rabbit Pab Sigma Aldrich). For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-tagged protein as verified by Western blot with an anti-His antibody (Sigma) were pooled and loaded onto the StrepTrap column ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Biophysics 2021Quote: ... or anti-His antibodies (Sigma- Aldrich).
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bioRxiv - Microbiology 2019Quote: ... or anti-His (1:1000 Sigma) overnight at 4 °C and anti-mouse secondary (1:5000) ...
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bioRxiv - Microbiology 2021Quote: ... α-His (Sigma, cat# H1029, 1: 3,000), α-ICDH (1 ...
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bioRxiv - Microbiology 2020Quote: ... α-His(Sigma, cat# H1029, 1: 10,000), α-HA (Santa Cruz ...