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Citations for Millipore Sigma :
251 - 300 of 3815 citations for Lymphocyte antigen 6H LY6H Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was performed boiling in pH6 Citrate buffer (Sigma-Aldrich) for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was followed by permeabilization with 0.5% Triton-X100 (Sigma). EdU incorporation was followed by EdU Click-IT chemistry according to manufacturer’s instructions (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse-anti-flavivirus group antigen (Millipore: MAB10216; RRID: AB_827205; 1:500), pan-flavivirus hyperimmune mouse acites fluid (HMAF ...
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bioRxiv - Cell Biology 2021Quote: ... SV40 T Antigen (Ab-2) (Merck Millipore; DP02; 5 μg/ml). Secondary antibodies (all from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Antigen retrieval was performed with pH 6.0 citrate (Sigma-Aldrich, C9999) and heating with a microwave for 25 min at 400 watts ...
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bioRxiv - Synthetic Biology 2019Quote: ... the antigen-antibody complex was visualized by chemiluminescent HRP substrate (Millipore).
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bioRxiv - Cancer Biology 2019Quote: ... mouse monoclonal antibody against the FLAG antigen (F1804, 1:2,000; Sigma), or with polyclonal antibody against β-tubulin (PA1-41331 ...
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bioRxiv - Neuroscience 2021Quote: ... and subjected to antigen retrieval using citrate buffer pH 6.0 (Sigma). Endogenous peroxidase activity was blocked for 10 min with BLOXALL solution (Vector labs) ...
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bioRxiv - Developmental Biology 2020Quote: ... tissue sections were submerged in 1x antigen retrieval citrate solution (Sigma), microwaved for 5 min on power 90 ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-neuronal nuclear antigen (NeuN, mouse: 1:200, Millipore, #MAB3771). The number and morphology of microglia in the hippocampi were assessed by staining for ionized calcium-binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with pH 6.0 citrate (Sigma-Aldrich, C9999) and heating with a microwave for 25 min at 400 watts ...
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bioRxiv - Immunology 2022Quote: ... Samples were immersed in EDTA Antigen Retrieval Buffer (pH 8.5, Sigma) on a hot plate and simmered for 25 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... antigen retrieval using hot 10 mM sodium citrate buffer (Sigma, S4641) supplemented with 0.05% Tween 20 (Bio Basic Canada ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen-retrieved sections were blocked using 5% donkey serum (Sigma; D9663) or 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... 350 µg antigen and 1000 μl Freund’s complete adjuvant (Sigma, F5881) were mixed and injected into the rabbits’ skin ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed using a 1x Citrate Buffer (Sigma, C9999) in a pressure cooker for 15 minutes on high ...
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bioRxiv - Cell Biology 2023Quote: ... Paraffin sections were then antigen-retrieved using citrate buffer (Sigma Aldrich) for 25 minutes at 100°C ...
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bioRxiv - Genetics 2023Quote: ... Antigens were detected by chemiluminescence (Luminata Forte Western HRP Substrate, Millipore) using an Azure c300 Gel Imaging System (Azure Biosystems chemiluminescence ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen–antibody complexes were revealed using the ECL detection system (Millipore) and detected using a Pxi imaging device (SynGene).
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected at 80–90% confluency using PEI transfection reagent (Sigma) with 2 mg/ml PEI per mg of DNA ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were transfected with plasmids using polyethylenimine according to manufacturer’s instructions (Sigma). Neurons were transfected by the Ca2+-phosphate method (53) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were seeded on glass coverslips coated with poly-L-ornithine (Sigma) in clear 12-well plates (3 × 105 cells/well ...
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bioRxiv - Cell Biology 2022Quote: HEK293 Flp-In T-REx stable lines were cultured in DMEM (Sigma, D5671) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were transfected with lentiviruses particles containing Pin1 shRNA (Sigma-Aldrich, TRCN0000010577) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected using Polyethylenimine (PEI,Sigma Aldrich; St. Louis, Missouri, USA). PEI and vector media were combined in a ratio of 5µL PEI and 0.5µg vector in 250µL of serum-free DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... HEK293 cells were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10% fetal bovine serum at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: HEK293 or 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293 cells and were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma) supplemented with 10% v/v foetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were seeded onto glass coverslips coated with Poly-L-Lysine (Sigma) and left to adhere overnight prior to transfection with plasmid DNA ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Microbiology 2020Quote: Intraepithelial and lamina propria lymphocytes were isolated from colon biopsies and stimulated in the presence of brefeldin A (Sigma) and CD107a antibody (eBioH4A3 ...
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bioRxiv - Microbiology 2020Quote: ... Vero cells engineered to over-express signaling lymphocyte activation molecule (SLAM) were supplemented with 0.4mg/mL of geneticin (G418, Sigma). Human foreskin fibroblasts (HFF-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... USA) with the mouse Tumor Dissociation kit (Miltenui Biotec) and lymphocytes were enriched on a Ficoll gradient (Sigma-Aldrich). T cells were stained using anti-CD3-Alexa Fluor 488 ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Neuroscience 2021Quote: ... antigen retrieval was performed in 1% citrate buffer (Sigma-Aldrich, Madrid, Spain) and 0.05% of Tween 20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... a commercial mouse anti-flavivirus group antigen monoclonal antibody (MAB10216; Merck Millipore) diluted 1:1,000 in phosphate-buffered saline (PBS ...
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bioRxiv - Pathology 2020Quote: ... Enzymatic antigen retrieval was performed using α-chymotrypsin (Sigma-Aldrich, MO, USA) for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and non-specific antigens were blocked with 10% goat serum (Sigma, UK). CD15 and CD15s primary antibodies were applied for one hour followed by incubation in secondary conjugates for 30 minutes ...
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bioRxiv - Physiology 2020Quote: ... Antigen retrieval was performed with 10 mM Sodium Citrate buffer (Sigma Aldrich) at pH=6-6.2 for 15 min at 95°C ...
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bioRxiv - Immunology 2021Quote: ... A prime dose of 20µg of antigen in TiterMax Gold (Sigma-Aldrich) was administered intramuscularly in the hind leg(s) ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval protocol were achieved using Citrate buffer pH 6 (Sigma-Aldrich). Sections were saturated during 30 minutes in PBS 10% Human Serum 0,3% Saponin and incubated at 4°C with primary antibody overnight in PBS 10% Human Serum 0,3% Saponin ...
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bioRxiv - Genomics 2021Quote: ... Monoclonal mouse Anti-Stage-Specific Embryonic Antigen-4 Antibody (Millipore, MAB4304,1:100), Polyclonal Goat Anti- Human/Mouse Oct-3/4 Antibody (R&D System ...