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Citations for Millipore Sigma :
1 - 50 of 3815 citations for Lymphocyte antigen 6H LY6H Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
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bioRxiv - Cell Biology 2021Quote: ... Cycloheximide (100 μg/ml, 6h, Sigma), Puromycine (1 μg/ml,2h ...
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bioRxiv - Developmental Biology 2019Quote: ... fixed for 6h in 4% paraformaldehyde (Sigma) and washed with 70% ethanol according to pathology platform standard protocols ...
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bioRxiv - Genomics 2019Quote: The cancer-derived human CD8+ T-lymphocyte cell line My-La CD8+ (Sigma-Aldrich) was cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Cell Biology 2024Quote: HEK293 (Human Embryonic Kidney, ECACC 96121229 from Sigma-Aldrich Chemie GmbH) were cultured in DMEM (Dulbecco’s modified Eagle’s medium PAN biotech ...
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bioRxiv - Neuroscience 2024Quote: ... a human nuclear-specific antigen [(EMD Millipore; MAB1281 (1:1000)] ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit polyclonal antibody against the human ZCCHC10 antigen (HPA038944, 1:2,000; Sigma), mouse monoclonal antibody against the FLAG antigen (F1804 ...
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bioRxiv - Immunology 2024Quote: ... followed 6h later by intraperitoneal challenge with LPS (L2630, Sigma) at 5 mg/kg ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293-ACE2 cells [HEK293 cells (ATCC CRL-1573) stably expressing human ACE2]22 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were treated with NAMPTi (50 nM for 6h; Sigma-Aldrich, SML1348). For cisplatin and NAMPTi dual treatment ...
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bioRxiv - Cell Biology 2021Quote: ... Cycloheximide (used at 100 µg/ml for 6h) was from Sigma-Aldrich.
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Neuroscience 2019Quote: ... and PAK/cofilin mechanism experiments used human embryonic kidney cells (HEK293) cultured in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Immunology 2020Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate in 1:5000 PBS ...
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bioRxiv - Immunology 2024Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate diluted in PBS at a ratio of 1:5000 ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Immunology 2022Quote: ... adipose tissue explants were cultured for 6h in DMEM/High Modified (D6429, Sigma) with 100U/ml Pen-Strep in the absence of FBS ...
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bioRxiv - Genetics 2020Quote: ... Parasites were synchronised with a 6h time window by 5% sorbitol lysis (Sigma) during ring stage ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells (Sigma:85120602) were cultured using DMEM (Gibco™ ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells (Sigma Aldrich) and were cultured in DMEM (Gibco ...
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bioRxiv - Immunology 2023Quote: ... OVA antigen (Sigma) was dissolved in saline to a 1 mg/mL concentration ...
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bioRxiv - Immunology 2020Quote: ... The human lymphocytes were isolated from fresh heparinized venous blood via centrifugation over Ficoll gradients (Histopaque-1077; Sigma-Aldrich, Gillingham, United Kingdom) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed 1X antigen retriever solution (Sigma, pH 6.0). Tissues were incubated with primary antibody in 5% donkey serum ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes underwent 6h incubation with primary antibodies: mouse α KCC2 (1:1000, Millipore; #07-432), rabbit α phospho-KCC2(S940 ...
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bioRxiv - Cancer Biology 2019Quote: ... 1% DMSO for 6h at 28°C and then with 1µg/µl Nocodazole (Sigma-Aldrich) for an additional 6h before preparing cell suspensions ...
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bioRxiv - Biochemistry 2021Quote: HEK293 and HEK293T cells (Sigma) were cultured at 37 °C ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma) and revealed using the enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
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bioRxiv - Immunology 2022Quote: ... Antibody production was conducted by co-transfection of the heavy and light chain expression vectors into Human HEK293 suspension culture cells (Expi293F) using polyethylene mine (Sigma). Cells were subsequently cultured for 4 days before being pelleted by centrifugation ...
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bioRxiv - Immunology 2021Quote: ... cultured lymphocytes were re-stimulated with 0.25μg/ml PMA (SIGMA) and ionomycin (SIGMA ...
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bioRxiv - Immunology 2020Quote: ... and lymphocytes isolated by using a Hisopaque-1119 (Sigma-Aldrich) density gradient ...
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bioRxiv - Immunology 2020Quote: ... and lymphocytes isolated by using a Hisopaque-1119 (Sigma-Aldrich) density gradient ...
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bioRxiv - Immunology 2021Quote: ... Lymphocytes were then isolated using a Histopaque-1119 (Millipore Sigma) and cells were suspended in 1x PBS containing 5% adult bovine serum (FACS buffer) ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were activated for >24h with 1µg/ml PHA (Sigma) and 150 U/ml IL2 (Eurobio ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 WT (Sigma Cat. Nr. 85120602), HEK293 GALNT1 KO (Narimatsu et al. ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (Sigma Aldrich 12022001-1VL) were maintained in DMEM supplemented with L-glutamine and 10% FBS ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed with pH 6.0 citrate buffer antigen retrieval solution (Sigma #C9999) in a pressure cooker and cooled for 20 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Immunology 2019Quote: ... Lymphocytes were isolated over a Percoll 67/44 gradient (Sigma-Aldrich). Bone marrow cells were purified using Lymphocyte Separation Medium (Lonza Bioscience ...
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bioRxiv - Immunology 2024Quote: ... Hepatic lymphocytes were enriched via 35% Percoll (Cytiva)/RPMI 1640 (Sigma) gradient centrifugation (700ξ g for 20min at RT ...
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...