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Citations for Millipore Sigma :
351 - 400 of 3815 citations for Lymphocyte antigen 6H LY6H Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Sections were deparaffinized and rehydrated and underwent antigen retrieval using Trilogy (Sigma-Aldrich) solution and a pressure cooker ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval in 1X Sodium Citrate Buffer (100mM trisodium citrate (Sigma, Cat#S1804), 0.5% Tween-20 (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... sections underwent antigen retrieval in trypsin (Sigma, #, 1g/ml, 10 minutes, 37°C). Sections were permeabilised in 0.25% triton for 30 minutes at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: sections were incubated 30 minutes in 1x antigen retrieval solution (Sigma-Aldrich C9999) at 80°C and washed three times in 1x PBS ...
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bioRxiv - Physiology 2024Quote: ... antigen retrieval was performed following rehydration by incubating with 0.8% hyaluronidase (Sigma-Aldrich) at 37°C for 30 min and antigen retrieval buffer (100 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed by boiling samples in citrate buffer (Sigma-Aldrich, C9999) for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2020Quote: ... and Hek293 (ATCC CRL-1573, embryonic kidney) were cultured in DMEM high glucose (Sigma-Aldrich), all supplemented with 2mM glutamine (Biochrome) ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293-GNTI cells were transfected with 1 mg DNA and 3 mg PEI (Sigma-Aldrich) per liter of cells at a density of 1.3-1.8E6 cells/ml ...
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bioRxiv - Cell Biology 2022Quote: SLC1A2/A3-expressing HEK293 Flip-In cells were plated on Poly-L-lysine hydrobromide (Sigma)-coated glass coverslips and induced with doxycycline (1 μg/ml ...
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Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineagebioRxiv - Microbiology 2021Quote: ... HEK293 and HZ1 cells were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells (ATCC number CRL-1573) were cultured in Dulbecco’s Modified Eagle’s medium (DEME) (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... SH-SY5Y and HEK293 cells were maintained in Dulbecco’s Modified Eagles Medium (DMEM; Sigma Aldrich) with 10% and 5% FBS respectively ...
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bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... The two lamina propria lymphocyte fractions were combined after centrifugation and further enriched by centrifugation in 40% Percoll® (Sigma #P1644) to remove epithelial cells and debris ...
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bioRxiv - Immunology 2021Quote: Lymphocytes were stimulated in vitro with PMA (50 ng/mL) and ionomycin (1 μg/mL) (both Sigma-Aldrich, St. Louis, MO) for four hours at 37°C in the presence of both Brefeldin A (GolgiPlug ...
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bioRxiv - Genomics 2019Quote: ... were isolated from leukopacks of anonymous blood donors purchased from the Gulf Coast Blood Center (Houston, TX) using Lymphocyte Separation Medium (Sigma-Aldrich). Cultures of PBMCs were stimulated with phytohemagglutinin PHA-P and IL-2 (50 U/ml ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...
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bioRxiv - Genetics 2021Quote: ... His-TCF7 was PCR cloned into pET52b (Millipore, 71554-3) using the primers 5’-CGGGGTACCTCATGCCGCAGCTGGACTCC-3’ and 5’-ATAGTTTAGCGGCCGCGAGCACTGTCATCGGAAGGAAC-3’ ...
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bioRxiv - Biochemistry 2021Quote: α-PCNA (P8825) and α-HIS (H1029) were purchased from Sigma; α-Cul2 (EPR3104 ...
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bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Anti-His primary antibody (H1029) was purchased from Sigma Aldrich, HRP conjugated secondary antibody (J1430 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Cancer Biology 2023Quote: ... which were cryopreserved in HI FBS with 10% DMSO (Sigma). Cells were directly transferred to a -80 °C freezer in Coolcell freezing containers (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% HI-horse serum and vancomycin (Sigma, USA). The inoculated flask was then kept in shaking conditions (100 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... and BRAF NTs/ BRAF KD with His antibody (Sigma SAB5600227). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% heat-inactivated fetal bovine serum (HI-FBS; Sigma) at 37°C for 15 ∼ 30 min ...
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bioRxiv - Microbiology 2024Quote: ... and heat-inactivated serum (HI-FBS; Sigma F9665, Lot# BCCB5091) was aliquoted and stored at −20°C until required.
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... The antibody-antigen complex was detected using HRP conjugated Immobilin Western Chemiluminescent substrate (Millipore). For JAZ3 degradation experiments in N ...
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bioRxiv - Neuroscience 2021Quote: ... Antigen-antibody complexes were visualized by enhanced chemiluminescence using Immobilon Western blotting kit (Millipore).
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bioRxiv - Cancer Biology 2021Quote: ... Antigen retrieval was performed by immersion in 95°C TRIS-EDTA (Sigma-Aldrich Ltd) comprised of 1M Tris-HCl (pH approximately 8.0 ...
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bioRxiv - Physiology 2021Quote: ... Antigen retrieval was performed by an incubation in 1x sodium citrate buffer (Sigma Aldrich) at 100°C for 30’ ...
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bioRxiv - Microbiology 2022Quote: ... antigen was mixed with an equal volume of in Titermax Gold Adjuvant (Millipore-Sigma), a water-in oil emulsion containing squalene ...
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bioRxiv - Microbiology 2022Quote: ... antigen was mixed with an equal volume of in Titermax Gold Adjuvant (Millipore-Sigma), a water-in oil emulsion containing squalene ...