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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... nuclei were counterstained with DAPI (4’,6-diamidino-2-phenylindole; 1:1000, Sigma). Following washes ...
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bioRxiv - Neuroscience 2024Quote: ... then stained with DAPI (41Z,6-diamidino-2-phenylindole; 1:1000; EMD Millipore) in blocking buffer for 10 minutes before three final washes ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-α tubulin (T5168, B-5-1-2, Sigma-Aldrich, 1:1000). Rabbit anti-Solo antibody was purified using an immunogen peptide conjugated-Sepharose column from the antiserum ...
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bioRxiv - Immunology 2022Quote: ... HLA-E expressing K562 cells were sorted and maintained in complete RPMI supplemented with 2 µg/mL blasticidin (Sigma-Aldrich, Belgium). All cells were cultured at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... S2 cells were cultured in Shields and Sangs M3 medium (Sigma cat# S3652) with 10% fetal bovine serum (Gibco cat#10437-010 ...
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bioRxiv - Bioengineering 2019Quote: ... hPBMCs were characterized via flow cytometry (Guava easyCyte 6HT, Merck Millipore; Table S2). Prior to cell seeding ...
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bioRxiv - Biophysics 2020Quote: ... Semi-adherent S2 cells were maintained in Schneider’s medium containing L-glutamine (Sigma) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg of S2 was mixed with 1 mg of polymyxin B (Sigma) immediately before subcutaneous deposition ...
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bioRxiv - Cell Biology 2021Quote: ... S2 cells and dissociated brain cells were fixed with 4 % paraformaldehyde (Sigma Aldrich) in PBS (pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: Drosophila S2 cells were cultured at 28°C in Schneider’s Insect Medium (Sigma) supplemented with 10% heat-inactivated bovine growth serum (HyClone) ...
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bioRxiv - Cell Biology 2024Quote: The monoclonal antibodies anti-pSer5 CTD S2 (Millipore, 04-1571, 1:500 dilution), anti-pSer5 CTD S5 (Millipore ...
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bioRxiv - Neuroscience 2024Quote: All dissections were performed in cold Schneider’s insect media (S2, Sigma Aldrich, #S01416) within a 15-minute window before solution exchange to avoid tissue degradation ...
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bioRxiv - Neuroscience 2024Quote: All dissections were performed in cold Schneider’s insect media (S2, Sigma Aldrich, #S01416). Brains were then fixed overnight at 4°C in 1% paraformaldehyde (20% PFA ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Microbiology 2024Quote: ... Concentration curves were done with guanine (0-1200 µM, Sigma Aldrich) or hypoxanthine (0-10000 µM ...
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bioRxiv - Neuroscience 2024Quote: ... using a subcutaneous injection of 200mg of MOG peptide (Myelin Oligodendrocyte Glycoprotein Peptide Fragment 35 to 55; from Charité) emulsified in complete Freund’s adjuvant (from Sigma-Aldrich) that contained 200mg of Mycobacterium tuberculosis H37RA (from Difco) ...
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bioRxiv - Microbiology 2019Quote: ... coli-nirS was negatively stained with freshly prepared 2% aqueous uranyl acetate on carbon-coated nickel grids (300 mesh) (Sigma). The excess stain was blotted ...
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bioRxiv - Systems Biology 2021Quote: ... coli strain and cultured the cells in M9 minimal medium (Difco) supplemented with 1/2 MEM amino acids solution (SIGMA) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA sequence of each was verified (University of Vermont Cancer Center Advanced Genome Technologies Core) and then transformed into Escherichia coli Rosetta 2(DE3)pLysS competent cells (Novagen).
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bioRxiv - Biochemistry 2020Quote: ... (2) LPS group: Mice received intranasal inhalation of 20 uL LPS (1 ug/uL, from Escherichia coli 0111:B4, Sigma) and i.v ...
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bioRxiv - Biochemistry 2022Quote: Streptococcus pyogenes Cas9 wild type protein and the nuclease dead mutant (D10A, H840A) were both recombinantly expressed for 16 hours at 18 °C in Escherichia coli Rosetta 2 (DE3) (Novagen) N-terminally fused to a hexahistidine affinity tag ...
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bioRxiv - Biochemistry 2022Quote: ... the expression levels of recombinant B56 subunit and PME-1 were monitored by western blot using antibodies that specifically recognize HA-tag (Sigma, 2CA5, Cat#: 116666060011, 1:1000) and PME-1(Abcam ...
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Mitochondrial ROS1 increases mitochondrial fission and respiration in oral squamous cancer carcinomabioRxiv - Cancer Biology 2020Quote: ... Anti-GAPDH (#G8795, 1:5000 for immunoblotting) and anti-myc tag (#05-724MG, 1:1000 for immunoblotting) antibodies were purchased from Sigma-Aldrich (St Louis, MO, USA). Anti-TOM20 (#sc-11415 ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml EGF (Sigma, E 4127) and 100 μl LIF(Millipore ...
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bioRxiv - Biochemistry 2020Quote: Anti-Japanese encephalitis E (mouse monoclonal, Millipore), influenza A H9N2 HA (mouse monoclonal ...
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bioRxiv - Molecular Biology 2020Quote: Gamma secretase inhibitor Compound E (Millipore Sigma) was dissolved in DMSO and diluted 1:1000 in RPMI media to a final concentration of 1 μM ...
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bioRxiv - Systems Biology 2021Quote: ... 10 μM E-64 (Sigma-Aldrich # 324890), 1.45 μM pepstatin (Sigma-Aldrich # P5318) ...
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bioRxiv - Microbiology 2020Quote: ... The cathepsin inhibitor E-64d (Sigma-Aldrich), the serine protease inhibitor camostat mesylate (Abcam) ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... Rabbit anti-CENP-E (C7488, Sigma Aldrich), diluted 1:100 ...
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bioRxiv - Biochemistry 2021Quote: ... Ergosterol obtained from Sigma-Aldrich (E-6625), cholesterol ester ...
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bioRxiv - Developmental Biology 2019Quote: ... 1.5 mg/mL ProNase E (Sigma P6911), 62.5 U/mL DNAse (Applichem ...
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bioRxiv - Biochemistry 2020Quote: ... pepstatin and E-64 (Sigma Aldrich, USA). Then ...
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bioRxiv - Bioengineering 2020Quote: ... Culture medium was Williams’ Media E (Sigma) supplemented with nicotinamide (12 mmol/L) ...
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bioRxiv - Developmental Biology 2022Quote: ... 1x β-mercaptoethanol (Millipore, ES-007-E), 1µM PD98059 (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 μM E-64 (Sigma, E3132) for protease inhibition ...
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bioRxiv - Developmental Biology 2023Quote: ... and 3.4 mM vitamin E (Sigma-Aldrich) (45) ...
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bioRxiv - Immunology 2024Quote: ... and stained with H&E (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... 3.6 μg/ml E-64 (Sigma-Aldrich), 5 mM EDTA (Calbiochem) ...
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bioRxiv - Molecular Biology 2023Quote: ... 20-hydroxyecdysone (20-E) (H5142; Sigma Aldrich) was resuspended to an initial stock concentration of 10 mM in ethanol.
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bioRxiv - Microbiology 2024Quote: ... with 10X E-Media (DMEM, Millipore-Sigma, #D7777-10L ...
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bioRxiv - Microbiology 2024Quote: ... and ethambutol (E) were purchased from Sigma. Pyrazinamide (Z ...
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bioRxiv - Microbiology 2022Quote: ... full-length InvL with a C-terminal His10 tag was expressed from pET-22b(+)::invL-his10 in Rosetta-gami 2(DE3) cells (Novagen, Madison, WI) using ZYM-5052 autoinducible media for 72 h at 20°C (103) ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated with the primary antibody (rat anti-serotonin, Chemicon MAB352, 5/1000; rabbit anti-tyrosine hydroxylase, Sigma Aldrich SAB2701683, 2/1000) in buffer (1% sheep serum ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).