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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The following compounds were used at the indicated concentrations: TR100 (5, 8 or 10 μM, Sigma Aldrich), CK666 (25 μM ...
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bioRxiv - Molecular Biology 2020Quote: ... worms were transferred to plates supplemented with compounds as described and 10 µM 5-fluorouracil (Sigma Aldrich). 10 µM 5-fluorouracil treatment continued for the first two weeks of life ...
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bioRxiv - Immunology 2023Quote: ... 5% CO2 followed by the addition of Brefeldin A to 10 μg/mL (Sigma Aldrich; B7651-5mg) and 5 hours of additional incubation at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mM Tris-HCl (pH7.4)] with ATP detection system containing 5 mM α-ketoglutarate (Sigma-Aldrich 75892), 0.01 mM Ap5A (Sigma D6392) ...
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bioRxiv - Immunology 2023Quote: ... protein samples were dried and reconstituted in 40 µl of 10% formic acid/5% DMSO (Sigma-Aldrich). Samples were analyzed as previously described [Van Herwijnen 2016] with a QExactive Plus instrument (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... TES buffer (diluted 1:5 in milli-Q H2O) (supplemented with 10 μL benzonase (Millipore cat# E1014), 2 mM MgSO4 and protease inhibitor cocktail (Roche cat# 04693132001) ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were then incubated with 10 ml blocking buffer (5% skim milk powder (Merck Millipore, 115363) in TBST ...
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bioRxiv - Neuroscience 2022Quote: ... or a mix of 10 μg/mL Laminin and 5 μg/mL CSPGs (Sigma-Aldrich Cat#CC117). All recombinant SLRP proteins used were produced in mammalian expression systems ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated at 65°C for 5 minutes and afterwards alkylated with 10 mM iodoacetamide (Sigma) for 20 minutes in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... MPI Münster) were maintained under 5% CO2 and 37°C conditions on 10 % gelatine-coated (Sigma, G1393) T25 flasks in DMEM high glucose medium (Sigma D5671 ...
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bioRxiv - Cancer Biology 2022Quote: ... S-phase populations were identified by incorporation of 10 μM 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) 45 min before fixation ...
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bioRxiv - Microbiology 2022Quote: ... were maintained in Dulbecco’s modified Eagle’s medium supplemented with 5% to 10% newborn calf serum (Millipore Sigma) and antibiotics (complete DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% or 10% fetal bovine serum (FBS; Sigma #F6178; Sigma-Aldrich Canada, Oakville, ON, Canada), respectively.
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bioRxiv - Cancer Biology 2023Quote: ... 5% glycerol) using the Amicon Ultra-15 centrifugal filter column with a 10 kDa pore size (Millipore). Protein was quantified with the Bradford method before adjusting to a stock concentration of 4 mg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Penicillin-Streptomycin-Neomycin (~5,000 units penicillin, 5 mg streptomycin and 10 mg neomycin/mL, Sigma Aldrich, US) and L-glutamine (1%) ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) (Biosera lot#11307) and 5% penicillin and streptomycin (Sigma Aldrich). HMSCs were expanded in T175 flask at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat#15102) were diluted in filtered DMEM containing 10% FBS and 5% Bovine Serum Albumin (BSA; Sigma). Incubations with the primary antibodies were performed at RT for 1 h ...
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bioRxiv - Plant Biology 2024Quote: ... cotyledons from 5-day-old seedlings were dissected and submerged in 10 μg/ml PI (Sigma–Aldrich) for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and compound were preincubated for 5 mins at room temperature before addition of 10□M ATP(Sigma) in 100mM Hepes pH7.4 (Sigma) ...
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bioRxiv - Pathology 2024Quote: ... and treated with or without 5 ng/mL TGFβ1 and GSK2193874 (2 and 10 μM, Sigma Aldrich) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Israel) for females were 5 mm long and contained 10% 17β-estradiol (E8875, Sigma, St. Louis, USA) in cholesterol (C3292 ...
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bioRxiv - Cancer Biology 2021Quote: ... Coverslips were washed with PBS for 5 minutes and then fixed in 4% paraformaldehyde for 30 minutes and permeabilized using 0.5% TritonX-100 (Sigma-Aldrich) for 5 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibodies were washed away with PBS (3x 5 min) whereafter 4’,6-diamidino-2-phenylindole (DAPI, 1:500, Sigma) was added for 10 minutes to localize the cell nuclei ...
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bioRxiv - Cancer Biology 2021Quote: ... They were fixed with a mixture of 4% paraformaldehyde and 5% sucrose for 10mins and permeabilised using 0.1% Triton X-100 in PBS (Sigma-Aldrich) for 5min at room temperature ...
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bioRxiv - Immunology 2021Quote: Three- to 4-week-old mice were injected intraperitoneally twice with 0.25 mg 5-ethynyl-2’-deoxyuridine (EdU; Sigma-Aldrich) 2 hours apart ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Three more washing steps with TBS were performed before resolving the staining with 5 ml of substrate per membrane: a 30 mg tablet of 4-chloro-1-naphthol (Sigma) dissolved in 10 ml of methanol ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated with nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) substrates (Sigma-Aldrich) in NTM (100mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... and the blotting signals were chemically visualized with either the nitro-blue tetrazolium/5-bromo-4-chloro-3′-indolyphosphate (NBT/BCIP) chromogenic assay (Sigma) or infrared scanner ...
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bioRxiv - Molecular Biology 2020Quote: ... 35.5 mM sucrose and 5 mM EGTA) and fixed in either 4% formaldehyde for 20 min or Bouin’s fixative (Sigma-Aldrich) for 2 min ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
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bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (5 μM) and propidium iodide (PI) (50 μg/mL) (Sigma-Aldrich, St. Louis, USA) and incubated at room temperature for 30-60 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... or 1000 nM of 2-(4-amino-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-1H-indol-5-ol (PP242; Sigma, P0037), (2 ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with antibodies (5-8 μg) overnight at 4°C followed by incubation for 3 hours with protein G-sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Bioengineering 2021Quote: ... a volume of 4 mL of top agar (TSB + 0.75% bacteriological agar, Scharlau) and 5 mM calcium chloride (Sigma-Aldrich) were added to the phage dispersion mixed with the bacteria ...
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bioRxiv - Bioengineering 2021Quote: ... we incubated them with suspended lentiviral solutions (MOI 3-5) for 24 h with 4 µg/mL polybrene (Sigma-Aldrich) before we replaced the medium with refresh culture medium ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in B2 with 2% nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP, Sigma) until adequate colour development ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µg/ml bovine insulin [Sigma], 4 µg/ml heparin [Sigma], 20 ng/ml basic fibroblast growth factor [bFGF, Sigma] ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at 4°C overnight with the following primary antibodies in 5% BSA in TBST: puromycin (Sigma, 1:25,000) and vinculin (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: C57BL/6N female mice (4-6 weeks old) were superovulated by injection with 5 IU of pregnant mare serum gonadotropin (PMSG) (Millipore), followed 5 IU of human chorionic gonadotropin (hCG ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5 ppt APS, 1.5 ppt TEMED, and (optionally, for thick tissue slices) 15 ppm 4-hydroxy TEMPO (4HT, Sigma, 176141). Monomer solution was made by combining all components of gelation solution except APS ...
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bioRxiv - Genomics 2021Quote: ... Samples were then centrifugated at 500g for 5 minutes at 4°C and then were resuspended in 1mL Nuclei PURE Storage Buffer (Nuclei PURE storage buffer, Sigma). Sample washing was performed until the supernatant cleared ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli DH5α cells were transformed with the resulting pho-lac fusion plasmids and streaked on dual indicator plates containing LB agar with 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) (Sigma) at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with a cytometry buffer solution (PBS with 5 % FBS) then fixed with a 4 % formaldehyde (Sigma-Aldrich) for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Fat bodies were dissected from larvae at 5 or 12 days AEL fixed in 4% PFA and counterstained with Nile Red (Sigma), Phalloidin-488 (Invitrogen ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... Membranes were incubated in primary antibodies (1:1000) overnight at 4° in 5% bovine serum albumin (BSA) (Sigma, A9647-100G), washed with TBST ...
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bioRxiv - Developmental Biology 2022Quote: ... adult DN mutant and Ctrl sibling zebrafish were bathed for 24 h in the presence of 5 μM 4-HT (H7904; Sigma) made from a 10 mM stock solution dissolved in 100% ethanol at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected by the addition of BCIP (5-bromo-4-chloro-3-indolylphosphate)-nitroblue tetrazolium solution (Sigma-Aldrich). The reaction was stopped after 2 min by washing the blots with large volumes of deionized water.
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bioRxiv - Bioengineering 2021Quote: ... slides were either treated with SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolulphosphate/Nitro blue tetrazolium, pH 9.5, Sigma) and counterstained with nuclear fast red (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Excess TMT reagent was quenched by the addition of 4 μl of an aqueous solution of 5% hydroxylamine (Sigma, 438227). Mixed peptides were subjected to a reverse phase clean-up step (OASIS HLB 96-well μElution Plate ...