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Citations for Millipore Sigma :
1351 - 1398 of 1398 citations for Cyclosporin A 13C2 d4 Acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... THP-1 monocytes were allowed to differentiate into macrophages by using 100 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, USA) [19] ...
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bioRxiv - Bioengineering 2024Quote: ... sGAG content was measured by first digesting tissue with 2% papain (Millipore Sigma, CAT:76216) (1 mL/construct, 0.56 U/mL in a buffer containing 0.1 M sodium acetate (Millipore Sigma, CAT: 71188), 10 M cysteine hydrochloric acid (Millipore Sigma ...
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bioRxiv - Bioengineering 2024Quote: THP1 cells were cultured in RPMI medium supplemented with 10% Fetal Bovine Serum with 5uM phorbol 12-myristate 13-acetate (Sigma-Aldrich P8139) for 3 days to differentiate them into macrophages ...
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bioRxiv - Cell Biology 2024Quote: ... Dissociated cells were collected and cultured in RPMI medium containing 200 nM TPA (12-O-tetradecanoylphorbol-13-acetate, Sigma-Aldrich, Germany, #P8139) and 200 pM cholera toxin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Proteolysis was performed using 2 μM of encapsulated or unencapsulated DypB in proteolysis buffer (100mM Sodium acetate pH 5, 2mM DTT) with or without the addition of 1 μM Cathepsin B (Sigma-Aldrich Cat# 219362). Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from the throat swabs collections stored as frozen PBS suspensions at −20°C using a manual TRI Reagent – chloroform extraction and sodium acetate – ethanol precipitation (Sigma-Aldrich, Poznań, Poland). Presence of SARS-CoV-2 material in the collected sample was tested using GeneFinder real-time COVID-19 plus kit (OSANG Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... medium, containing 3% sucrose, 10−6 M potassium indole acetate (Nacalai Tesque, Kyoto, Japan) and 10−5 M kinetin (Sigma, St. Louis, MO) at 25 °C ...
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bioRxiv - Neuroscience 2022Quote: ... we used 10 μl amyl acetate (AM, Sigma-Aldrich, Cat. No: STBF2370V, diluted 1:50 in paraffin oil-Sigma-Aldrich, Cat. No: SZBF140V) and octanol (OCT ...
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bioRxiv - Microbiology 2022Quote: ... The peptide mix was then reconstituted in 50 mM sodium acetate pH 5 and treated with 0.25 U/mL Clostridium perfringens neuraminidase (Sigma-Aldrich Cat. Nr. N3001) at 37 °C for 3 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... The digested product was analysed on a 2% agarose gel in 1X Tris-Acetate EDTA (TAE) buffer (Sigma-Aldrich, Saint Louis, MO, USA) to highlight wt or lakritz mutant corresponding fragments ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Retinoic acid (RA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) used for cell differentiation were purchased from Sigma-Aldrich (R2625 and P8139, respectively). According to our previous work (Carmona et al. ...
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bioRxiv - Biochemistry 2020Quote: ... U937 and THP-1 cells were pre-stimulated with 100ng/ml Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) for 1h in RPMI-1640 medium containing 1% FBS.
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bioRxiv - Physiology 2021Quote: Muscle fibers were impaled with 2 sharp microelectrodes filled with 2 M potassium acetate solution containing 1 mM sulforhodamine 101 (Sigma-Aldrich, Catalogue #S7635) to allow for visualization ...
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bioRxiv - Immunology 2020Quote: ... Sample separation was performed using a C8 column and solvent system consisting of ammonium acetate (5 mM, Sigma Aldrich, St Louis, MO, USA) combined with acetic acid (0.1% ...
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bioRxiv - Immunology 2020Quote: Equine PBMCs (4 × 106 cells) were cultivated with 20 ng/mL of phorbol 12-myristate acetate (PMA; Sigma–Aldrich, St. Louis, MO, USA) and 1 μg/mL of ionomycin (Sigma–Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Cells (~106/ well) were seeded into wells of a 24-well dish and activated with 25 nM phorbol myristate acetate (PMA) (Sigma-Aldrich, MO, USA) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were first stimulated with phorbol myristate acetate (PMA, 50ng/mL, Fisher BioReagents, cat. #50-058-20001) and ionomycin (750 ng/mL, Millipore Sigma, cat #AAJ62448MCR) in the presence of GolgiStop (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ and TNF-α expression was measured by stimulating cells with 10 ng/ml phorbol 12-myristate 13-acetate (PMA) and 100 μg/ml ionomycin (both Sigma-Aldrich, München, Germany) for 4 hours in the presence of 5 μg/ml Brefeldin A (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Monocyte-derived macrophages were derived by treating the cell lines with 100 ng/ml of phorbol 12-myristate 13-acetate (PMA; Sigma, St. Louis, MO) for 48 h followed by a 24 h incubation in medium alone before experiments ...
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bioRxiv - Microbiology 2024Quote: ... 100 μl of serum was mixed with 100 μl of acetate buffer (0.2 mol/L, pH 5.0) containing 100 units of ß-glucuronidase/aryl sulfatase (Cat. No. 1041140002, Sigma-Aldrich, St. Louis, MO) and 50 μM of the internal standard 4-HBP (Cat ...
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bioRxiv - Immunology 2022Quote: ... dispersed cells were cultured for 4 h in RPMI/10% fetal calf serum (FCS) with 1.5 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (all from Sigma-Aldrich, St Louis, Mo) with Golgi-Stop (BD Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... the meshes were washed with three drops of distilled water and stained for one minute with uranyl acetate (Sigma, St Louis, MO, US) at a concentration of 1% ...
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bioRxiv - Biophysics 2022Quote: THP-1 cells were cultured in the cell culture media described before with 20nM phorbol 12- myristate 13-acetate (PMA) (Sigma Aldrich, Ref. P1585) for 48h in glass-bottom Petri dishes ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5-ml glass vials (Ø×H 11.6 × 32 mm) containing ∼ 100 mg glass wool were filled with 200 µl (Z)-3-hexenyl acetate (HAC, >98%, Sigma-Aldrich, Buchs, Switzerland) and sealed with screw caps containing a rubber septum ...
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bioRxiv - Neuroscience 2024Quote: ... we used 10 µl amyl acetate (AM, Sigma-Aldrich, Cat. No: STBF2370V, diluted 1:50 in paraffin oil-Sigma-Aldrich, Cat. No: SZBF140V) and octanol (OCT ...
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bioRxiv - Immunology 2024Quote: THP-1 monocytic cells were differentiated into macrophages after 24 hours of stimulation with phorbol 12-myristate 13-acetate (PMA) 100 ng/mL (Sigma Aldrich, P8139-1MG), then polarized into M1 or M2 macrophages after 48 hours of culture with 20 ng/mL interferon-γ (INF-γ ...
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bioRxiv - Biochemistry 2024Quote: ... 5x 105 cells/well were seeded into 6-well plates and incubated with 20 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma/Merck, Darmstadt, Germany) in RPMI1640 medium containing 10% v/v heat-inactivated FCS for 24 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... THP-1 derived macrophages were obtained by differentiating THP-1 cells with phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) treatment at 100 nM for 24 h according to the previous study (Li et al. ...
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bioRxiv - Immunology 2022Quote: ... The supernatant of both freshly sorted UTCs and UTC-derived clones after 24 hours of stimulation with phorbol myristate acetate (PMA, 1 ng/mL; Sigma-Aldrich, 16561-29-8) + ionomycin (0.5 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Dexamethasone (DEX, cat. #D4902), prednisolone (PRED, cat. #P6004), and medroxyprogesterone 17-acetate (MPA, cat. #M1629) were all purchased from Sigma-Aldrich (St. Louis, MO).
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bioRxiv - Cancer Biology 2020Quote: ... was prepared from 1 × 106 neutrophils cultured in 1 mL of D10 medium with or without phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, now MilliporeSigma, P8139) for 4 h ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 cells were adjusted to 5×105 viable cells/ml and placed into fresh medium with 100 ng/ml phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, St. Louis, MO) to induce differentiation into a macrophage-like state ...
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bioRxiv - Immunology 2021Quote: ... for 4 hours in the presence or absence of Phorbol Myristate Acetate (PMA) and Ionomycine with Brefeldin A (all from Sigma Aldrich, St Louis, MO) and Golgi stop (BD Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... was defined as the amount of enzyme required to hydrolyze the substrate to produce 1 mol of p-nitrophenol per min. p-Nitrophenyl acetate C2 (CAS No. 830-03-5) was from Sigma-Aldrich (St.Louis, MO, USA).
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bioRxiv - Genomics 2023Quote: ... 5 ml of tagmentation wash buffer (TMG) was prepared consisting of a final concentration of 10 mM Tris Acetate pH 7.5 (Sigma 93352 and Sigma A6283, respectively), 5 mM MgAcetate (Sigma M5661) ...
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bioRxiv - Immunology 2024Quote: Cells from draining popliteal lymph nodes (1 x 106) were stimulated for 4 h at 37 °C with PMA (phorbol 12-myristate 13-acetate, 10 ng/mL, Sigma-Aldrich, Darmstadt, He, Germany) and Ionomycin (10 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... . Ultrathin sections placed on grids were stained with 1% aqueous uranyl acetate (Agar Scientific, Stansted, UK) and 3% lead citrate (Sigma Aldrich, St. Louis, USA), and observed at the FEI Morgagni 268D microscope.
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were plated in 48 well plates at 150,000 cells per well with 20 nM phorbol 12-myristate 13-acetate (PMA) (P8139-1MG ; Sigma-Aldrich, St. Louis, MO, USA) supplemented in RPMI-1640 media +10% FBS and +1% penicillin-streptomycin to induce differentiation to monocyte-derived macrophages (dTHP-1) ...
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bioRxiv - Microbiology 2024Quote: ... BC-3 cells were seeded at a density of 0.5×106 cells/mL and treated with 20 ng/mL TPA (12-O-tetradecanoylphorbol-13-acetate; MilliporeSigma 5244001MG) dissolved in dimethylsulfoxide (DMSO; Sigma Aldrich Fine Chemicals Biosciences). Reactivation rates were checked for all experiments and are plotted in Supp ...
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bioRxiv - Microbiology 2024Quote: ... 5×105 cells/well were seeded in 48-well plates and incubated in RPMIc with 200nM PMA (Phorbol 12-myristate 13-acetate, Merck Sigma, Saint Quentin Fallavier, France). After 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... PBMCs were either left unstimulated or activated for 3 days by adding phorbol 12-myristate 13-acetate (PMA) and ionomycin (Sigma-Aldrich Canada, Oakville, ON) (P/I ...
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bioRxiv - Bioengineering 2024Quote: ... calcium carbonate (CaCO3; Ref. 21069), δ-gluconic acid lactone (GDL, Ref. G4750) and ethyl acetate (Ref. 34858) were purchased from Sigma-Aldrich (Buchs, Switzerland). PBS was purchased from Thermo Fisher Scientific and endotoxin-free water was used for in vivo experiments (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: Hep-2 target cells were incubated with increasing concentrations of anti-EGFR antibodies (0 to 500 ng/mL) in the presence of Jurkat CD16 cells and the protein kinase C activator phorbol- myristate acetate (P8139, Sigma-Aldrich, St Louis, MO, USA). After 16 h of incubation at 37°C ...
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bioRxiv - Genomics 2020Quote: ... were stimulated for 48 hours with PMA+ionomycin (phorbolmyristate acetate 25 ng/ml, Fisher Scientific, Hanover, IL; ionomycin calcium salt, 1 μg/ml, Sigma-Aldrich, St. Louis, MO) and treated with hydrocortisone (28 nmol/l ...
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bioRxiv - Physiology 2023Quote: ... 1.106 cells were transferred to a 96-well round-bottom plate and stimulated for 5 h with 50 ng/mL of phorbol-12-myristate-13-acetate and 1 µg/mL of ionomycin (Sigma-Aldrich, Saint Quentin Fallavier, France) with brefeldin A (10 µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Isoluminol (# A-8264), dimethyl sulfoxide (DMSO, #D2650), Latrunculin A (#L5163) and PMA (phorbol 12-myristate 13-acetate, #P8139) were from Sigma-Aldrich (Merck, Burlington, MA, USA) whereas horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2022Quote: ... THP-1 monocytes were stimulated to differentiate into macrophage-like cells by treatment with 20 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich Co., St Louis, MO) for 18-20 h at 37°C and incubated further for 48 h to allow differentiation (Bhaskar et al. ...
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bioRxiv - Bioengineering 2022Quote: ... SLS-graft-PLGA (SLS-PLGA) was dissolved in ethyl acetate (EtOAc, organic phase) and calcium peroxide (CaO2, Cat# 466271, Sigma-Aldrich, St. Louis, MO, USA) and dissolved under stirring at room temperature ...