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Citations for Millipore Sigma :
1001 - 1050 of 1398 citations for Cyclosporin A 13C2 d4 Acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... IFNγ and TNFα production were assessed by flow cytometry following stimulation of 1×106 whole splenocytes with 0.1μg/ml of phorbol 12-myristate 13-acetate (PMA, Sigma P8139) and 1μg/ml of Ionomycin (Sigma I0634 ...
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bioRxiv - Bioengineering 2020Quote: ... THP-1 cells were differentiated into macrophage-like cells with 150 nM phorbol 12-myristate 13-acetate (PMA) (Sigma) before the experiment.
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was discarded and proteins were digested by resuspension in 100 μL 100 mM ammonium acetate with 10 mM dithiothreitol and 0.5 μg trypsin (Proteomics grade, Sigma), and incubation at 37 °C with shaking for 16 h.
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bioRxiv - Neuroscience 2020Quote: ... Periorbital thresholds were assessed each hour for 5 hours following acetate (60 mg/kg i.p. Sigma, St Louis, MO) administration ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampi tissues were manually homogenized in 300 µl ChABC buffer (40 mM Tris-HCl pH 8.0, 40 mM sodium acetate, C3667, Sigma) containing Benzonase supplemented with Halt protease/phosphatase inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 cells differentiated with 100 ng/mL phorbol-12-myristate 13-acetate (PMA) (Sigma-Aldrich, CAT No.:79346) were allowed to adhere for 24 hours before infection ...
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bioRxiv - Cancer Biology 2021Quote: ... in 100 mM Na-acetate buffer (pH 5.9)/0.2 M NaCl with or without pepstatin A (200 µM; Sigma-Aldrich) in the presence of phosphatidylcholine (0.12 mM ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... were used at 14,000 x g and 4 °C to exchange purified protein samples to 150 mM ammonium acetate (99.99 %; Sigma-Aldrich), pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The mixture was cleared by a 5 minute spin through a Corning Costar Spin-X filter (0.45 μm cellulose acetate, Sigma) at 20000 x g and room temperature ...
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bioRxiv - Microbiology 2021Quote: ... J-Lat latent proviruses were reactivated by adding 10 µM of phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) to the culture media for 18 hours ...
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bioRxiv - Microbiology 2020Quote: ... THP-1s were plated on 8-well Nunc Lab-Tek II chamber slides (Thermo-Fisher) at a density of 150,000 cells per well with 100nM PMA (phorbol 12-myristate 13-acetate, Sigma) in growth media and incubated at 37°C and 5% CO2 for 24 h ...
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bioRxiv - Immunology 2020Quote: ... To induce monocyte to macrophage differentiation cells were plated at 0.4×106/mL) in complete media supplemented with 25 ng/mL phorbol 12-myrisate 13-acetate (PMA, Sigma) for 24 hours ...
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bioRxiv - Biochemistry 2021Quote: ... One milliliter of the N-oxide solution was added dropwise to 3 mL of sodium acetate (37.5 mM; pH 5.3; Sigma Aldrich), followed by incubation at room temperature for 24 hours ...
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bioRxiv - Synthetic Biology 2020Quote: Styrene was quantified by diluting 20 μL of dodecane overlay into 200 μL of ethyl acetate containing 0.5 mM trans-caryophyllene (Sigma) as an internal standard ...
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bioRxiv - Bioengineering 2020Quote: ... and adjusted to the desired volume with 100 mM Sodium acetate (pH 5) (Sigma-Aldrich, Saint Louis, MO, USA). 100 µL sample was mixed with 400 µL reaction mix and incubated at 50 °C with 1500 RPM shaking until a blue color had developed in the samples with LsAA9A ...
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bioRxiv - Synthetic Biology 2021Quote: ... Purified VLP samples were diluted to 0.1-0.2 mg/ml in PBS and filtered with 0.22 μm cellulose acetate spin filters (Sigma-Aldrich #CLS8161). 25 μl of each sample was injected through a Bio SEC-5 2000 Å HPLC column (Agilent ...
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bioRxiv - Biophysics 2021Quote: ... EMT was induced by incubating cells in medium supplemented with 100 nM 12-O-tetradecanoylphorbol-13-acetate (TPA) (PN:P8139, Sigma) for 48 hours prior to measurement [47] ...
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bioRxiv - Immunology 2021Quote: ... PMA (phorbol 12-myristate 13-acetate) (50 ng/mL) and ionomycin (400 ng/mL) (Sigma, St. Louis, Missouri, USA) stimulated hWB were treated with the following compounds AST-VII ...
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bioRxiv - Biochemistry 2021Quote: ... The MIDAS screening pools were prepared in LC-MS grade 150 mM ammonium acetate pH 7.4 (Sigma-Aldrich 73594) and pH-adjusted with ammonium hydroxide (Sigma-Aldrich 338818) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... rehydrated slides were incubated in filtered pH 5.32 acetate buffer containing 1 mg/ml Naphthol-AS-TR-phosphate (Sigma), 100 mM Sodium Tartrate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... 12-O-Tetradecanoylphorbol 13-acetate (TPA, Cat#P8139) and sodium butyrate (NaB, Cat#AAA1107906) were purchased from Sigma-Aldrich and Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... processed into single cell suspensions and stimulated with 50 ng/mL of phorbol 12-myristate 13-acetate (PMA, Sigma) and 1 μg of ionomycin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... treated with phorbol 12-myristate 13-acetate (PMA, 2.5 μg/ml) and ionomycin (0.25 ng/ml, both from Sigma-Aldrich). After incubation ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated for 4h at 37°C with PMA (phorbol myristate acetate; 0.5 μg mL-1; Sigma Aldrich) and ionomycin (1 μg mL-1)(Sigma Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stimulated with 50 μL of 10−7 M phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) or N-formyl-methionyl-leucyl-phenylalanine (fMLP ...
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bioRxiv - Immunology 2021Quote: All samples for CD-MS measurements were first buffer exchanged into 500 mM ammonium acetate solution (pH 7.5) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore), unless otherwise stated ...
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bioRxiv - Neuroscience 2022Quote: ... and it was centrifuged at 400 g for 5 min to remove any cells before filtering through a cellulose acetate membrane with 0.45 μm pore size (Millipore). The supernatant was snap-frozen in dry ice before storing it at -80°C ...
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bioRxiv - Immunology 2023Quote: ... They were differentiated by treatment with 200 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, P8139–5MG) for 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... where sodium acetate (pH=5.8) was added to a final concentration of 0.3M and 500µl of isopropanol (Sigma-Aldrich, France). Samples were left overnight at −20°C and then centrifuged for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... intracellular cytokine production was analyzed after restimulation with 100 ng/mL phorbol myristate acetate (PMA) and 1μg/mL ionomycin (both Sigma) for a total 6h ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE was performed on pre-cast Tris-Acetate gels followed by transfer to PVDF transfer membrane (Merck Millipore). Proteins were detected using primary antibodies as described followed by incubation with secondary antibodies coupled with HRP (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Biophysics 2023Quote: Samples for native mass spectrometry were exchanged into 50 mM ammonium acetate (pH 6.8) using 10 kDa cut-off Amicon Ultra centrifugal filtration units (Merck Millipore). Samples were introduced to a first-generation Waters Synapt QToF (Waters Corporation ...
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bioRxiv - Microbiology 2023Quote: ... 100,000 THP- 1 cells were treated with 125 nM phorbol 12-mysristate 13-acetate (PMA, Sigma-Aldrich, CAT# P1585) in complete RPMI-1640 medium for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... containing 1% (w/v) of poly(vinyl acetate-co-alcohol) (PVA; MW 13–23 kDa, 98% hydrolysed; Sigma-Aldrich) as stabiliser ...
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bioRxiv - Immunology 2023Quote: ... Both the native fab and the deglycosylated fab were buffer exchanged into 150 mM ammonium acetate (pH 7.5) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore). The samples were loaded into gold-coated borosilicate capillaries (in-house prepared ...
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bioRxiv - Immunology 2023Quote: ... for 1 hour followed by 3 hours of stimulation using 50 nM phorbol myristate acetate (PMA, Sigma-Aldrich, P1585), 25 μg/ml Lipopolysaccharide (LPS ...
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bioRxiv - Cell Biology 2023Quote: ... EMT was induced by incubating cells in medium supplemented with 100 nM 12-O-tetradecanoylphorbol-13-acetate (TPA) (PN:P8139, Sigma) for 48 h prior to measurement [26] ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated in vitro for 5 hr with phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, 50ng/mL), calcium ionophore A23187 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... The aqueous mobile phase A consisted of 95:5 (v:v) water:acetonitrile supplemented with 20 mmol/L ammonium acetate and ammonium hydroxide (Sigma-Aldrich). Mobile phase B was 100% acetonitrile ...
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bioRxiv - Molecular Biology 2024Quote: ... Nickel-3,3’-diaminobenzidine (Ni-DAB) solution (0.175M Sodium acetate, 1% Nickel ammonium sulphate, 50mg DAB (Sigma-Aldrich, D-5905)) with 0.075 % H202 was used to develop the colour for 6 min stopping the reaction by washing the sections 2 x 5 min in dH2O ...
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bioRxiv - Neuroscience 2024Quote: ... two out of four different odor cues (citral, 1-butanol, isoamyl acetate, and L-carvone; Sigma-Aldrich, MO, USA) were paired with a reward (2 ul of water ...
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bioRxiv - Molecular Biology 2023Quote: ... pregnant females were ovariectomized on E2.5 and afterwards injected with 3 mg medroxyprogesterone 17-acetate subcutaneously (Sigma, M-1629) on E3.5 and E5.5 ...
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bioRxiv - Immunology 2024Quote: ... + 10% FBS (S181B-500, Biowest) were differentiated using 10 ng/mL PMA (phorbol 12-myristate 13-acetate, P8139, Sigma) for 24 h ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: Recombinant human MMP-2 (R&D) and MMP-9 (R&D) were activated using p-aminophenylmercuric acetate (APMA, Sigma) in MMP assay buffer (50 mM Tris (Fisher) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 200 μL of the upper (dodecane) layer was sampled and diluted with 800 μL of ethyl acetate (Sigma Aldrich). The mixture was mixed for 5 min and centrifuged at 12,500 g for 10 min at 4°C to remove any residual aqueous phase and yeast cells ...
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bioRxiv - Physiology 2023Quote: ... The supernatant was added to a freshly prepared solution of Alcian blue (0.4%) in sodium acetate (0.5M) (33864-99-2; Sigma-Aldrich), magnesium chloride hexahydrate (30 mM) ...
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bioRxiv - Plant Biology 2023Quote: Powder resulting of MSC surface abrasion was chemically extracted in the collector tube with extraction buffer (80 μL of 5 mM sodium acetate pH 4.6 containing 1 μL.mL−1 of plant protease inhibitor cocktail (Sigma P9599) per mg of powder) ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells were differentiated to macrophages with 100 ng/mL phorbol-12-myristate 13- acetate (PMA, Sigma-Aldrich) for 3 days ...