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Citations for Millipore Sigma :
1301 - 1350 of 1398 citations for Cyclosporin A 13C2 d4 Acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... THP-1 monocyte differentiation was initiated by incubating the cells with 10 ng/mL phorbol 12-myristate 13- acetate (Sigma-Aldrich) for 2 days at 37°C in a 5% CO2 environment ...
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bioRxiv - Immunology 2024Quote: ... the cells were stimulated with 500 nM ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) (both from Merck Sigma-Aldrich), and BD GolgiPlug Protein Transport Inhibitor (containing Brefeldin A ...
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bioRxiv - Immunology 2024Quote: ... THP-1 monocytes are differentiated into macrophages by 24 h incubation with 150 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139) followed by 24-48 h incubation in fresh RPMI medium ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions from murine tissues were cultured ex vivo at 37°C for 2.5 hours in RPMI 1640 media (supplemented as above and with 10% fetal bovine serum) with 50 ng/mL phorbol myristate acetate (PMA) (Sigma-Aldrich), 5 μg/mL Ionomycin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: For ex vivo stimulation one third of Percoll-isolated brain leukocytes were incubated for 4h at 37°C with Phorbol 12-Myristate 13-Acetate (PMA) (50 ng/ml) and ionomycin (1000 ng/ml) (Sigma-Aldrich) in presence of monensin (Golgistop ...
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bioRxiv - Immunology 2024Quote: ... THP1 monocytes were differentiated to macrophages after 3-h exposure to 50 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma–Aldrich) in 1 mL of growth medium ...
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bioRxiv - Immunology 2024Quote: ... followed by dehydration using an ethanol series (contrasting with uranyl acetate at the 30% stage) and embedding with an Epoxy Resin Kit (Sigma-Aldrich). Ultrathin sections were cut on a Leica UC6 ultramicrotome contrasted with lead citrate and images were acquired on a FEI Spirit Biotwin ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/ml of brefeldin A and 0.05 μg/ml of phorbol 12-myristate 13-acetate (Sigma Aldrich Millipore, Oakville, Canada) for four-hours at 37 □C at 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/ml of brefeldin A and 0.05 μg/ml of phorbol 12-myristate 13-acetate (Sigma Aldrich Millipore, Oakville, Canada) for four-hours at 37 □C at 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... THP-1 cells were differentiated for 48 hours with 100 nM phorbol myristate acetate (PMA, Sigma-Aldrich, Cat. No: P8139– 5MG), followed by priming for 4 hours with 1 μg/mL lipopolysaccharides (LPS ...
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bioRxiv - Biochemistry 2024Quote: ... was dissolved in 1 ml 0.2 M acetate buffer (pH 5.6) containing 40 mM CaCI2 and 30% 13C-glycerol (w/w) (Sigma-Aldrich, Cat#489476). Forty micrograms of 1,2-dioleoyl-PC were dissolved in 1.7 ml diethyl ether ...
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bioRxiv - Microbiology 2024Quote: ... THP1-b monocytes were previously induced to differentiate into macrophages using 150 nM PMA (Phorbol 12-myristate 13-acetate; Sigma, P8139). Samples were fixed using Pierce 4% PFA (ThermoFisher) ...
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bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
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bioRxiv - Immunology 2024Quote: ... were seeded in 6-well plates (1.5×106 cells/well) with or without phorbol 12-myristate 13-acetate (PMA, 100 ng/ml, 2 ml/well, Sigma-Aldrich) and incubated at 37°C for 48 hrs ...
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bioRxiv - Biochemistry 2024Quote: ... then glycogen was precipitated followed by enzymatic digestion using 0.3 mg/mL amyloglucosidase in 0.2 M sodium acetate buffer at pH 4.8 (Sigma 10115, 70 U/mg). Total glucose was quantified using 1H NMR then total glycogen was expressed in glucose units per mg of liver tissue.
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bioRxiv - Biochemistry 2024Quote: ... Post-exposure baking at 95 °C for 5 min was then followed by developing with propylene glycol methyl ether acetate (PGMEA, Sigma-Aldrich) and a washing step with isopropanol (IPA ...
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bioRxiv - Bioengineering 2024Quote: ... filtered through a 0.45 μm cellulose-acetate filter and then concentrated with a 100 kDa Amicon filter (Sigma, cat. no. UFC910008) at 2500 g for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... T cells were re-stimulated using a combination of phorbol 12-myristate 13-acetate (PMA) (50ng/ml) (Sigma-Aldrich, Cat: P8139) and Ionomycin (1μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µL of cell culture media was analysed in duplicate by incubation with TRAcP reaction buffer (1 M acetate (Sigma-Aldrich), 0.5% Triton X-100 (Sigma- Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... cells were seeded at 0.5 × 106 cells mL-1 in 75 cm2 tissue culture flasks and stimulated with 50 ng mL-1 of PMA (phorbol 12-myristate 13-acetate; Sigma-Aldrich). After 24 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We treated samples that initially failed to PCR amplify with sodium acetate and then ethanol precipitated with GenElute LPA Linear Polyacrylamide (Sigma-Aldrich) to increase DNA concentration 67 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell pellets were suspended in 500 µl of IP buffer (20 mM HEPES-KOH, pH 7.6, 125 mM potassium acetate, 1 mM EDTA, 20% glycerol, 1mM DTT, 1% NP-40, 1x Sigma protease inhibitor cocktail). One ml of acid-washed glass beads was added to each tube and the cells were lysed by bead beating for 8 minutes with incubation on ice for 3 minutes after each minute ...
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bioRxiv - Genomics 2020Quote: ... cells were grown to a density of 8.0 × 105 cells/mL and treated with 25ng/mL Phorbol 12-Myristate 13-Acetate (PMA) (Sigma-Aldrich, Catalogue #P8139) for 4 days ...
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bioRxiv - Microbiology 2021Quote: ... The degraded products of the dye in the filtrate were extracted with an equal volume of ethyl acetate (Sigma Aldrich; HPLC grade) and dried over sodium sulphate (Na2SO4) ...
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bioRxiv - Genomics 2021Quote: ... The tissue was mechanically lysed with a scalpel in a total volume of 5 ml lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Biochemistry 2020Quote: ... methanol (LC-MS grade), 2,5-dihydroxyacetophenone (DHA, ≥99.5%, Ultra pure), and anhydrous sodium acetate (NaOAc, ≥99%) were all purchased from Sigma Aldrich (Zwijndrecht, The Netherlands) and used without further purification.
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bioRxiv - Neuroscience 2021Quote: ... Baseline fluorescence was recorded for 30s before application of either vehicle or 1μM phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich, Cat# P8139) and imaged every 10s for 5 minutes at 50% laser power ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Systems Biology 2020Quote: ... For macrophage differentiation U937 cells were suspended in medium with 20 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, #P1585) and plated in 96-well microplates with µClear®flat bottom (Greiner ...
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bioRxiv - Cancer Biology 2020Quote: HL60 cells were plated at a density of 4×105 cells/ml and differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA) (Sigma, Darmstadt, Germany) at a final concentration of 32 nM for 6 days ...
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bioRxiv - Cancer Biology 2020Quote: ... It was mechanically dissociated using a scalpel in 1 ml of lysis buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were re-suspended in 5 ml washing buffer (0.32 M sucrose [Sigma-Aldrich 84097], 5 mM calcium dichloride [Sigma-Aldrich 21115], 3 mM magnesium acetate [Sigma-Aldrich 63052] ...
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bioRxiv - Microbiology 2022Quote: ... and macrophage-like differentiation was induced upon a twenty-four hours treatment with 100ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma cat. P1585). Calu-3 cells media was instead supplemented with a 1x final concentration of non-essential amino-acids (Sigma cat ...
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bioRxiv - Immunology 2020Quote: ... cell suspensions in RPMI medium supplemented with 10% FBS were re-stimulated for 4 hr with phorbol 12-myristate 13-acetate (PMA, Sigma, 50ng/mL) and ionomycin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated in complete RPMI media containing 200 ng/mL of Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, #P8139) for 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... Jurkat Timer clones were treated for 24 h with a combination of T-cell stimulants: phorbol 12-myristate 13-acetate (SIGMA, Cat# P1585) at a final concentration of 20 ng/ml and ionomycin (Nacalai Tesque ...
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bioRxiv - Plant Biology 2022Quote: ... Relative quantification of all the VOCs was done by normalizing the peak area of the analytes with that of the internal standard nonyl acetate (Sigma-Aldrich, India).
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Molecular Biology 2024Quote: ... VFLIP for narrow window selection was buffer exchanged into 200 mM ammonium acetate using 50 kDa molecular weight cut-off (MWCO) Amicon® Ultra Centrifugal Filter (Sigma Aldrich). Samples were run at approximately 1 µM complex concentration for native mass spectrometry ...
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bioRxiv - Microbiology 2024Quote: ... cell differentiation into macrophages was induced by adding 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) to the medium for 72 hours.
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bioRxiv - Microbiology 2023Quote: ... Corresponding water samples were taken by filtering 2-L of tank water through 0.22-μm pore-size cellulose acetate membranes (Millipore Sigma, Burlington, MA). Samples were stored at −80°C until processing.
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bioRxiv - Microbiology 2022Quote: wtKSHV inoculum was obtained from BCBL-1 cells (5×105 cells/ml) induced with 20 ng of TPA (12-O-tetradecanoylphorbol-13-acetate; Sigma, St.Louis, USA)/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were seeded at a density of 1x105 cells/ml in 96 well plates and differentiated for 48 hours with 25 nm PMA (phorbol-12-myristate-13-acetate, Sigma-Aldrich, #P1585). Cells were then left to rest for 24 hours in fresh media containing no PMA prior to bleomycin exposures.
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well cell culture-treated plates and differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P1585) for 72 h in serum-free RPMI 1640 medium (SFM ...
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bioRxiv - Genomics 2022Quote: ... The total culture was then extracted through rigorous shaking with an equal volume of ethyl acetate (Sigma-Aldrich, St. Louis, MO, USA). The organic layer was removed and dried over anhydrous MgSO4 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... TC-HSMAM1 and Cas9-TC-HSMAM1 cells were maintained in a complete RPMI-1640 medium and 100 ng/mL of phorbol 12-myristate-13-acetate (PMA) (Sigma-Aldrich, USA) at 37°C for 48 h to induce cell differentiation to macrophages 31 ...
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bioRxiv - Plant Biology 2024Quote: The growth media was exchanged with 50 ml of filter-sterilized 20 mM Ammonium acetate pH 5.7 (102308585, Sigma-Aldrich, Missouri, USA) as described (McLaughlin et al. ...
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bioRxiv - Immunology 2024Quote: Purified T-cell subsets were incubated at 37°C for 2 hours in the presence or absence of phorbol 12- myristate 13- acetate (PMA, 50 ng/ml; Sigma Aldrich, U.S.A.), ionomycin (500 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... THP-1 monocytes were differentiated into macrophages by incubation with 100 ng/ml Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P1585) for 24 h ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 1-2 × 106 cells/mL in a final volume of 20 mL and treated with either 100 nM of phorbol 12-myristate 13-acetate (PMA) (Sigma Cat#P1585), or an equivalent volume of Dimethyl sulfoxide (DMSO ...