Labshake search
Citations for Millipore Sigma :
1251 - 1300 of 1398 citations for Cyclosporin A 13C2 d4 Acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... 100U/mL penicillin and 50 μg/mL streptomycin and differentiated into macrophages after treatment with 50 ng/mL phorbol-12-myristate-13-acetate (Sigma Aldrich) for 48 hours(42 ...
-
bioRxiv - Immunology 2023Quote: ... The size of the PCR product was predicted as 274 bp and confirmed in a 2% agarose gel in 1x Tris Acetate-EDTA (TAE) buffer (Sigma, T9650), stained by ethidium bromide (MP Biomedicals ...
-
bioRxiv - Cell Biology 2023Quote: THP1 cells were differentiated into macrophages by treating 1M cells /mL with 0.5 μM phorbol 12-myristate 13-acetate (PMA; Sigma; cat P8139) for 3 h on Day 0 ...
-
bioRxiv - Immunology 2023Quote: ... Intracellular cytokine production was analyzed using flow cytometry after 4 hours of polyclonal restimulation with 100 ng/mL phorbol myristate acetate (PMA, P8139, Sigma-Aldrich) and 1 µg/mL ionomycin (I0634 ...
-
bioRxiv - Microbiology 2023Quote: THP-1 human monocytes were differentiated into macrophages through a 24-hour (h) stimulation with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) in RPMI 1640 supplemented with 10% human AB serum ...
-
bioRxiv - Immunology 2023Quote: ... Leukocytes were then stimulated with 1 µg/ml each of phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO), ionomycin (Sigma Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... These cells can be matured to become robust pro-inflammatory cytokine-producing cells by Phorbol 12-Myristate 13-Acetate (PMA, Sigma-Aldrich). Transduced-THP-1 cells were incubated with PMA (200 ng/mL ...
-
bioRxiv - Genomics 2023Quote: ... 5 ml of tagmentation wash buffer (TMG) was prepared consisting of a final concentration of 10 mM Tris Acetate pH 7.5 (Sigma 93352 and Sigma A6283, respectively), 5 mM MgAcetate (Sigma M5661) ...
-
bioRxiv - Immunology 2023Quote: ... cultured cells were restimulated on day 5 with 50 ng/ml Phorbol 12-myristate 13-acetate (PMA) and 750 ng/ml ionomycin (both from Sigma-Aldrich) in the presence of GolgiPlug and GolgiStop (both from BD ...
-
bioRxiv - Immunology 2023Quote: ... and restimulated with 50 ng/ml Phorbol 12-myristate 13-acetate (PMA) and 750 ng/ml ionomycin (both from Sigma-Aldrich) in the presence of GolgiPlug and GolgiStop (both from BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... One well per NHP was stimulated with 10 pg/mL phorbol myristate acetate (PMA) and 1 μg/mL ionomycin (Sigma-Aldrich) (P+I ...
-
bioRxiv - Neuroscience 2022Quote: ... Peptides were washed two times with 200 µl 1% TFA 99% ethyl acetate (ThermoFisher Scientific; Cat#: 28904; Millipore Sigma; Cat#: 270989) followed 200 µl 0.2% TFA/5%ACN (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: Protein stock solutions were dialyzed against 200 mM aqueous ammonium acetate pH 7.4 using an Amicon 0.5 mL micro concentrator with a MW cutoff of 10 kDa (EMD Millipore, Billerica, MA) and stored at 4 °C until needed ...
-
bioRxiv - Immunology 2022Quote: ... cells were stimulated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL) and ionomycin (750 ng/mL; both Sigma-Aldrich) in the presence of GolgiStop (4 μl/6mL ...
-
bioRxiv - Molecular Biology 2024Quote: ... THP-1 monocyte differentiation was initiated by incubating the cells with 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma Aldrich) for 2 days at 37°C in a 5% CO2 environment ...
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... Host cell lines included U937 monocytes (ATCC CRL-1593.2) which were differentiated into macrophages with 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) and A ...
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... cells were plated on 24-well plates at 3x105 cells/well with 0.1 μg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) to promote differentiation ...
-
bioRxiv - Cell Biology 2024Quote: ... 106 THP-1 cells were transferred to each of the six-well plates and cultured with 100ng/mL phorbol 12-myristate 13-acetate (PMA) (P8139, Sigma-Aldrich) for 24h ...
-
bioRxiv - Microbiology 2024Quote: ... collected on carbon coated copper grids (EM Resolutions, UK), and stained sequentially with 2 % aqueous uranyl acetate (BDH, UK) and 0.5 % aqueous lead citrate (Sigma-Aldrich, UK). Sections were examined and imaged in an FEI Talos F200C TEM at 200 kV.
-
bioRxiv - Cell Biology 2024Quote: ... THP-1 cells were plated to 24-well plate at a concentration of 2 × 105 viable cells per well and incubated for 48 h in 1 ml/well of media supplemented with 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for differentiation into macrophages ...
-
bioRxiv - Cell Biology 2023Quote: ... The sections were stained with 0.4% uranyl acetate for 10 min and lead stain solution (Sigma-Aldrich, St. Louis, MO, USA) for 2 min and coated with osmium tetroxide using an osmium coater (HPC-1SW ...
-
bioRxiv - Microbiology 2023Quote: ... THP-1 monocytes were differentiated to macrophages with medium containing 50 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma #P1585) for 3 days ...
-
bioRxiv - Immunology 2023Quote: ... and 10-12 were treated with 100 µL of embryo water plus 1 µg/mL H2DCFDA and 400 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich). The plates are covered with aluminum foil and incubated at 28°C for 2.5 hours ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... using the PEG/Lithium acetate method (Yeast Transformation Kit, MilliporeSigma)(Gietz and Woods 2002) and plated on non-inducing selective synthetic media (Sigma-Aldrich® Yeast Synthetic Drop-out Media Supplements ...
-
bioRxiv - Systems Biology 2024Quote: ... the decidualization were induced by adding the following reagents into cell medium: Medroxyprogesterone 17-acetate (Sigma, M1629; final conc. 1.0 µM), E2 (estradiol ...
-
bioRxiv - Immunology 2023Quote: ... 4°C for 5min prior to stimulation in complete RPMI-1640 supplemented with 50ng/ml phorbol 12-myristate 12-acetate (PMA; Sigma-Aldrich), 1µg/ml ionomycin (Sigma-Aldrich) ...
-
bioRxiv - Microbiology 2023Quote: ... 5 × 105 cells/mL were seeded in the wells of flat-bottom tissue culture plates and matured into macrophages with 20 nM phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) during 72 h ...
-
bioRxiv - Microbiology 2023Quote: Cells were treated as indicated in the figures or figure legends with the following compounds: PMA (phorbol 12-myristate 13-acetate, Sigma Aldrich), Valproic acid (Sigma Aldrich) ...
-
bioRxiv - Systems Biology 2023Quote: ... Then the pellet was resuspended in 1 mL 100 mM sodium acetate (pH 4.5) supplemented with amyloglucosidase powder (Sigma-Aldrich, 10115) to a final concentration of 35 U/mL ...
-
bioRxiv - Immunology 2022Quote: ... Macrophages were generated by differentiating THP-1 monocytes with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) (Millipore Sigma, P8139) for 24 h ...
-
bioRxiv - Bioengineering 2022Quote: ... Pt was deposited by DC magnetron sputtering (150 nm) followed by lift-off in propylene glycol monomethyl ether acetate (PGMEA, Sigma-Aldrich) during sonication (20 min) ...
-
bioRxiv - Cell Biology 2022Quote: ... the following day the media was changed to DMEM media containing 10% FS or 10% dFBS supplemented with 100 □M acetate and 10% deuterium oxide (Sigma 151882). For glucose tracing ...
-
bioRxiv - Cell Biology 2022Quote: ... HESC were treated with differential medium (DMEM/F12 with 2% CS-FBS) containing 1 μM Medroxyprogesterone 17-acetate (MPA, Sigma, M1629), and 0.5 mM dibutyryl cAMP (dbcAMP ...
-
bioRxiv - Genomics 2022Quote: ... frozen brain tissue was disaggregated by pipetting up and down in chilled extraction buffer consisting of Poly(1-vinylpyrrolidone-co-vinyl acetate) (Sigma #190845), 0.1% TritonX-100 ...
-
bioRxiv - Immunology 2022Quote: ... T cells were stimulated with 50 ng/ml phorbol 12-myristate 13-acetate (PMA) and 500 ng/ml ionomycin (Sigma-Aldrich) in the presence of GolgiStop (BD ...
-
bioRxiv - Immunology 2022Quote: ... THP-1 monocytes are differentiated into macrophages by 36 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139). The murine macrophage Raw 274.7 cells (ATCC ...
-
bioRxiv - Molecular Biology 2022Quote: ... human monocytic cell-lines into macrophages was performed by treatment with 30 nM phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) for up to 24 hours ...
-
bioRxiv - Microbiology 2023Quote: U937-DC-SIGN cells were treated for 12 days with 160 nM Phorbol12-myristate-13-acetate (PMA) (Sigma-Aldrich®: P1585) and 30 ng/mL macrophage-colony stimulating factor (M-CSF ...
-
bioRxiv - Immunology 2023Quote: ... lymph nodes and splenic cell suspensions were stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, #P8139), 1 µM Ionomycin (Sigma-Aldrich ...
-
bioRxiv - Immunology 2023Quote: ... THP-1 cells were differentiated into phenotypically adherent macrophages by stimulation with 100 nM Phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) in RPMI complete ...
-
bioRxiv - Immunology 2022Quote: ... cells were first restimulated in vitro with 50ng/mL phorbol 12-myristate 13-acetate (PMA) + 2 μg/mL ionomycin (both Sigma-Aldrich) in the presence of Monensin (BD Biosciences ...
-
bioRxiv - Immunology 2023Quote: ... Both ex vivo and IL-25-stimulated cells were cultured in complete RPMI medium in the presence of phorbol-12-myristate-13-acetate (500 ng/ml) (Sigma-Aldrich) and ionomycin (250 ng/ml ...
-
bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated for 3.5 hours at 37°C with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 mM ionomycin (both from Sigma-Aldrich) in the presence of protein transport inhibitor cocktail (eBioscience ...
-
bioRxiv - Molecular Biology 2024Quote: ... and THP-1 (ATCC® TIB-202) human monocytic cells into macrophages was performed by treatment with 3 nM phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) for up to 24 hours ...
-
bioRxiv - Microbiology 2024Quote: THP1 (ATCC TIB-202) monocytes (4.75x105 cells/mL) differentiated into macrophages in phorbol 12-myristate 13-acetate (PMA, 20 ng/ml; Sigma-Aldrich, P1585) were infected with culture of bacteria at an OD600nm of 0.5 at MOI 100 ...
-
bioRxiv - Microbiology 2024Quote: ... on day -1 and +1 post oral fungal infection mice were given a subcutaneous injection of 150 mg/kg cortisone acetate (Sigma-Aldrich). For inoculation ...
-
bioRxiv - Biochemistry 2024Quote: ... 50 μL of soluble fraction was incubated with 50 μL of cathepsin B reaction buffer (50 mM sodium acetate, 4 mM EDTA, 8 mM DTT, 50 μM Z-RR-AMC (Sigma, C5429), pH 6 ...
-
bioRxiv - Microbiology 2024Quote: ... THP1 were differentiated for 48 hrs in macrophage media supplemented with 50 ng/mL Phorbol 12-myristate 13-acetate (in DMSO, Sigma-Aldrich). PMA-containing media was replaced with fresh macrophage media and cells were rested overnight prior to infection.
-
bioRxiv - Microbiology 2024Quote: ... THP-1 cells were differentiated into macrophage-like cells by treatment with 100LJng/ml phorbol 12-myristate 13-acetate PMA (Sigma-Aldrich). Overnight bacterial growths were diluted 1:100 in BHI medium and grown until an OD600nm ≈ 1 ...
-
bioRxiv - Microbiology 2024Quote: ... Eggs were then stained with 1% uranyl acetate en bloc at the 30% stage and embedded in the Epoxy Resin Kit (all from Sigma-Aldrich).