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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (diluted in 5% BSA in 1X PBS-T) were as follows: Mouse anti-β-Actin (1:10,000, Sigma-Aldrich, A5441), Rat anti-α Tubulin (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked in 5% milk for 1 hour and then incubated with primary antibodies for Tubulin (CST, 1:5000) and acetylated Tubulin (Sigma, 1:5000) in PBS supplemented with 3% BSA and incubated overnight on a rolling platform at 4°C ...
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bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... then three times for 5 minutes each then placed in Donkey Anti-Guinea Pig IgG Antibody-HRP Conjugate (Millipore-Sigma Burlington, MA) diluted 1:10,000 in Bløk-CH Noise Cancelling Reagent for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... 75 μg of sample was incubated at 4°C overnight on rotator with 5 μg mouse anti-puromycin antibody (Millipore Sigma: MABE343), or rabbit anti-eIF4E (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... and probed with the following antibodies (diluted in 5% milk in PBST) overnight at 4 °C: α-Tubulin (1:400000, Sigma t9026), α-Dnmt3a (1:5000 ...
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bioRxiv - Genomics 2023Quote: ... we used the crosslinking of 5’ azide-modified 18-nt DNA oligonucleotides (Integrated DNA Technologies) to lysine residues on antibodies via DBCO-PEG4-NHS (Sigma-Aldrich 764019) cross-linker with modifications from previous protocols82 ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-FLAG M2 antibody (1:40,000, Sigma, Thermo Scientific™). overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
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bioRxiv - Cell Biology 2023Quote: ... human ephrin-B2-Fc (10 µg/ml, RnD Systems, #7397-EB) was mixed with Cy3-conjucated Fc-antibody (5 µg/ml, Sigma-Aldrich, # C2571) in HBSS and incubated for 30-60 minutes at room temperature with gentle agitation ...
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bioRxiv - Neuroscience 2023Quote: ... Tubulin was detected by incubating for 1 h at room temperature in 5% Milk-PBST (Marvel) with an α-tubulin antibody (Sigma T9026) at a 1:40.000 dilution.
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin from about 1.5×106 cells was incubated overnight at 4°C with 2–5 μg antibodies to FLAG epitope (M2; Sigma-Aldrich, #F1804, RRID:AB_262044), BRD4 (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2024Quote: ... blotted and incubated overnight in 5% skim milk (GERBU Biotechnik, 70166) in TBST at 4°C with an anti-RAS antibody (Millipore, 05-516). As a loading control ...
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bioRxiv - Bioengineering 2021Quote: ... Western blot analysis was performed using a primary antibody of rabbit anti-protein A (1:25,000 dilution) (Sigma-Aldrich, St. Louis, MO) and a secondary antibody of goat anti-rabbit IgG-HRP (1:3,000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS at 37°C for 30 minutes and subsequently incubated for two hours with a 1:200 concentration anti-glial fibrillary acidic protein (GFAP) antibody produced in mouse (Sigma, 1:200) and 1:500 concentration anti-β-Tubulin III antibody produced in rabbit (Sigma ...
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bioRxiv - Immunology 2021Quote: Transiently expressed antibodies were purified from 30 ml of HEK 293 Expi cultures using Protein G GraviTrap columns (Sigma, GE28-9852-55) and the Ab Buffer Kit (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... SDS-PAGE and western blot analyses were used to detect proteins and modifications using the following antibodies: anti-phospho SMAD2 (Ser465/467, Sigma, 1:500), Smad2 Polyclonal Antibody (Life Technologies 1:500 ...
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bioRxiv - Neuroscience 2022Quote: Proteins were resolved by SDS-PAGE and proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The ratio of protein level or phosphorylation levele against control β-actin protein level (detected with a mouse monoclonal Anti-β-Actin-Peroxidase antibody (A3854 Sigma)) was normalized to untreated wt sample mean.
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bioRxiv - Cell Biology 2022Quote: Anti-His tag and microtubule-associated protein 1 light chain 3 beta (LC3) Antibodies were purchased from Millipore Sigma (Burlington, MA, USA). Anti-Adipose Differentiation-Related Protein (ADRP ...
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bioRxiv - Microbiology 2019Quote: ... Relative TLR3 protein expression levels between WT and CRISPR modified cells were obtained by measuring TLR3’s expression against the intracellular β-Actin control protein bound to an anti-β-Actin monoclonal antibody (1:300; Sigma). Protein detection in WES was accomplished according to the manufacturer’s protocol using streptavidin-HRP based methodology (ProteinSimple) ...
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bioRxiv - Pathology 2020Quote: ... Proteins were immunoblotted with antibodies against FECH (rabbit, 1:400, LS Bio, #LSC409953, and β-actin (mouse, 1:5000, Sigma-Aldrich, AC40). Secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Genomics 2021Quote: ... Eluted proteins were then analyzed by western blotting with anti-U1-70k mouse monoclonal antibody (9C4.1) (05-1588, Merk Millipore, Burlington, MA, USA) at a dilution of 1:500 for the detection of U1-70k ...
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bioRxiv - Molecular Biology 2021Quote: ... TMG-capped RNAs were isolated from RNase H-treated immunoprecipitates with Protein G Dynabeads coated with an α-TMG antibody (Sigma, MABE302), according to 59.
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bioRxiv - Cell Biology 2021Quote: ... Samples were boiled in 2x sample buffer for 5 min to elute the proteins and analyzed by SDS-PAGE followed by Western blot using anti c-myc antibody (Sigma-Aldrich, #C3956).
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bioRxiv - Microbiology 2020Quote: ... Proteins were separated by SDS-PAGE and visualized by Western blot analysis using primary antibodies for FLAG (Clone M2, Sigma-Aldrich, F1804) and HA (Roche ...
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bioRxiv - Immunology 2020Quote: ... Total protein in the cell lysate was estimated using the Lowry assay and 30 µg total protein from each cell lysate was used for immunoblotting with anti-HA antibody (Cat# H9658, Sigma, 1:10000), anti-AU1 antibody (Cat# ab3401 ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocytosis was assessed by glial fibrillary acidic protein (GFAP) staining using a Cy3-conjugated anti-GFAP antibody (1:200; C 9205, Sigma, Oakville, ON). Brain levels of Bdnf and Htt were determined by western blotting using protein lysates from either whole brain or microdissected brain sections with the following antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... Sixty μl of Dynabeads Protein G were incubated for 1h30 at 4°C under agitation with 16 μg of the anti-Ago2 antibody (Sigma-Aldrich, SAB4200085) in 1X PBS containing 0.1% of Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... The slices were then incubated overnight at 4ºC with primary antibodies for myelin basic protein (1:250 Millipore Cat# MAB384-1ML, RRID:AB_240837), neurofilament 160 kD medium (1:250 ...
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bioRxiv - Microbiology 2024Quote: ... and the immunoprecipitated proteins were eluted with Laemmli buffer and subjected to SDS-PAGE and immunoblotting with anti-Flag antibody (Cat# F1804, Sigma, 1:1,000), anti-HA antibody (Cat # H6908 ...
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bioRxiv - Microbiology 2024Quote: ... The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012, Sigma, 1:1,000). Loading control for cell lysate was detected with anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with mouse monoclonal antibodies against NeuN protein (Anti-NeuN, clone A60; 1:20K dilution; Millipore, St. Louis, MO, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... Western immunoblotting to confirm protein expression was performed as stated above using anti-FLAG HRP-conjugated antibody (Sigma Aldrich, A8592; 1:15,000) to verify transfection and expression of FLAG-RGS14.
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bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Genomics 2023Quote: ... the anti-TOMM20 antibody and the ConA protein were functionalized for click chemistry with the addition of a NHS-SS-DBCO (DBCO) (Sigma Aldrich #761532) molecule that subsequently reacted with the azide groups linked to their respective fluorophores ...
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bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 hours at RT and then immunoblotted overnight at 4°C with the primary mouse anti-amyloid precursor protein A4 antibody (clone 22C11) (Sigma MAB348-AF647) (1:3000 ...
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bioRxiv - Immunology 2023Quote: ... To ensure efficient and reproducible protein immobilization a preliminary array validation was carried out with anti-FLAG antibodies (Sigma-Aldrich, cat# F7425) 1:5000 and mouse anti-His6 tag polyclonal antibodies (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... An antibody reactive for the N-terminal amino acids 66-81 of the amyloid precursor protein (APP) (Clone 22C11: Millipore, Billerica, MA) was applied at 1:40K and incubated overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... the nitrocellulose membranes containing the purified recombinant fusion proteins were incubated with mouse anti-His antibody [clone HIS.H8] (Millipore Sigma, Burlington, Massachusetts). HRP-conjugated rabbit anti-mouse IgG antibody (SouthernBiotech ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained with 5 μL of Annexin V–FITC and 5 μL of propidium iodide (PI, Sigma; 5 μg/mL) for 15 min at room temperature in the dark ...
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bioRxiv - Cancer Biology 2021Quote: HDMB03 cells (3×105 in 6-well plates) were treated with global de-methylating agent 5-AzaC (5 μM) (5-Aza-2-deoxycytidine; Sigma). Following 96 hours of incubation ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were treated with 1 x 10-5 M 5-fluoro-2-deoxy-uridine and with 1 x 10-5 M uridine (Sigma) for 3 days ...
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bioRxiv - Microbiology 2023Quote: The small interfering RNAs (siRNAs) against NLRP3 (siNLRP3) (5’-UGCAAGAUCUCUCAGCAAA-3’) and the corresponding control siRNAs (siControl) (5’-UUCAAUAAAUUCUUGAGGU-5’) were synthesized from Sigma. The siGenome smart pool siRNAs and siON Target plus siRNAs were purchased from Dharmacon and are composed of a pool of four siRNAs ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were permeabilized with 0.5% Triton-X100 in PBS for 5 min at RT and a blocking solution (PBS supplemented with 5% BSA (Sigma) and 0.1% Triton-X100 ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 10µM baseclick 5-Ethynyl-deoxyuridine (5-EdU) (Sigma) for 2hrs or 20µM O-propargyl-puromycin (OPP ...
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bioRxiv - Biophysics 2021Quote: Pieces (5 mm X 5 mm) of silicon wafer disc (Sigma-Aldrich) were thoroughly washed with 100 % ethanol and stored in 70 % ethanol at room temperature until use ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with 5 μM of 5-FU (Sigma, Ref: F6627) alone or in combination with KDM6A/Bi or EZH2i for indicated days.