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Citations for Millipore Sigma :
1201 - 1250 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged proteins were probed with immunoblotting using monoclonal anti-Flag M2-Peroxidase (HRP) antibody (Sigma-Aldrich, Saint Louis, MO, USA) (1/50,000X dilution ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were transferred onto nitrocellulose membrane using iBlot2 Gel Transfer Device and immunostained using Neurexin-1 antibody (Millipore ABN161-I) and Tuj1 (Biolegend 801201 ...
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bioRxiv - Microbiology 2019Quote: ... 3×FLAG- and GST-tagged proteins were detected with a mouse monoclonal HRP-conjugated anti-3×FLAG antibody (M2; Sigma-Aldrich) and a rabbit polyclonal anti-GST antibody (Sigma-Aldrich) ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred to a PVDF membrane and incubated with one of the following primary antibodies: α-FLAG polyclonal rabbit (Sigma) or α-RpoA monoclonal mouse (Biolegend) ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Zoology 2020Quote: ... Each lysate was confirmed to express the appropriate recombinant protein at the expected size using an anti-V5 antibody produced in rabbit (Sigma V8137). Isoform specificity was tested via immunoblotting all cell lysates (empty vector control ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with protein A/G agarose beads (Pierce, Waltham, MA, USA) conjugated to antibodies against FLAG-tag (Sigma-Aldrich) or Exo70 (Santa Cruz ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... 30 confirmed transgenic plants were screened for dPPRrbcL expression by immunoblot on leaf total protein extracts using HA antibodies (H9658 clone, Sigma-Aldrich) and three lines expressing gradual levels of dPPRrbcL protein were chosen for phenotypic analyses.
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bioRxiv - Microbiology 2019Quote: ... Detection of FLAG-tagged proteins transferred onto a PVDF membrane was performed with monoclonal anti-FLAG M2-peroxidase (HRP) antibodies (Sigma-Aldrich) and Lumi-Light Western Blotting Substrate Kit (Roche) ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein samples were resolved using 10% SDS-PAGE and analyzed by Western blotting using anti-c-Myc antibody (Sigma-Aldrich, USA) and anti-PAX9 antibody (Cell signalling technology ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-ubiquitin proteins antibody (clone FK2; Enzo, New York, USA, Cat#BML-PW8810 and Sigma, Gothenburg, Sweden, Cat#04-263), guinea pig polyclonal anti-VGLUT1 (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... to preclear samples for 2 hours and subsequently incubated with Dynabeads protein G coated with an anti-Flag antibody (Sigma, F1804). The lysate-beads mixture was incubated at 4°C with rotation for 2 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Equal amounts of protein were subject to SDS-PAGE and blotted with anti-Rho antibody (3 μg/mL, #05-778, EMD Millipore) or anti-GAPDH antibody (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of lysate proteins were incubated with 2 µl of anti-Flag monoclonal antibody pre-adsorbed on protein A-Sepharose beads (cat. no. A2220, Sigma-Aldrich) overnight at 4°C.
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... diluted 1:5000 to detect RVFV and anti-Gc protein antibody (4C12A1) diluted 1:5000 to detect LACV with FFA staining buffer (1X PBS, 1mg/ml saponin (Sigma: 47036)) as a primary detection antibody overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2022Quote: ... Chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated with the indicated antibodies or rabbit preimmune sera complexed with Protein A agarose beads (Sigma-Aldrich). Immunoprecipitated complexes were sequentially washed with Wash Buffer A ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... correct production of the protein of interest was checked by Western Blot of HEK293T-transfected cells using an anti-Flag antibody (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation of nicks were carried out according to standard ChIP protocols using Protein G-coupled Dynabeads coated with monoclonal anti-biotin antibodies (Sigma-Aldrich) antibody ...
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bioRxiv - Microbiology 2023Quote: ... The upper part was used for protein detection using a specific monoclonal antibody against Cas9 (1:1000 dilution, #MAC133, Sigma-Aldrich) and Alexa555 secondary antibody for fluorescence detection (iBright ...
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bioRxiv - Cell Biology 2022Quote: ... Hxk2-3V5 and associated proteins were immunoprecipitated from glass bead extracts (250 μg total protein) using 20 μL of agarose conjugated anti-V5 antibody (Sigma; A7345). The extract and beads were incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... The samples for mass spectrometry (MS) analysis were incubated with Dynabeads protein G that were precoupled with the M2-Flag-antibody (Sigma, F1804) at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged protein was detected by incubating the milk powder-blocked membrane with mouse monoclonal anti-FLAG primary antibody (Sigma-Aldrich) diluted at 1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then incubated overnight with primary antibodies against Yes-associated protein (YAP) (mouse monoclonal anti-YAP, 1:400, Sigma-Aldrich) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein loading was determined using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000) from Sigma-Aldrich (A3854). Quantitation of western blot band intensity was done using Image J software.
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bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Bioengineering 2023Quote: We verified the patterning and the pattern transfer to PA hydrogel using green fluorescent laminin (as described in Protein patterning glass coverslips) and a pan-cadherin primary antibody (Sigma, C3678). We diluted the pan-cadherin antibody 1:200 in PBS and incubated on the devices for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... and proteins were detected by Western blot using monoclonal anti-Flag M2 antibody produced in mouse (1:5000 dilution; Sigma-Aldrich), rabbit anti-FKBP12 antibody (1:1000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated with HRP-tagged secondary antibodies and protein bands were detected using ECL Prime western blotting system (Catalog No. GERPN2232, Millipore Sigma). Protein band density was measured using ImageJ 1.52a ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies and chromatin were mixed with 20 µl of Magna-ChIP® protein-G magnetic beads (EMD Millipore, Cat: 16-662) for 2 hours at 4°C ...
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bioRxiv - Genomics 2024Quote: ... The following primary antibodies are used for the first target protein at the dilutions indicated: NeuN (1:500, A60, Mouse, Millipore MAB5374), pTDP43 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Cells were pelleted and supernatant incubated with 7.5 µg precipitating antibody and 100 μL Protein A Sepharose slurry (Sigma-Aldrich, P3391) (5% w/v in lysis buffer) ...
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bioRxiv - Genetics 2022Quote: ... The lysate was centrifuged at 4,000 x g for 5 min at 4°C and the protein pellet was resuspended in Digestion Buffer (10% 2,2,2-Trifluoroethanol [Sigma; #96924] in 100 mM HEPEs pH 8.5). Protein was quantified with BCA (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... in a well volume of 100 μL of a peptide substrate that includes the nsp5 cleavage site between nsp4 and nsp5 proteins (nsp4-5) (-AVLQ ↓ SGFR[K(DNP)]K-NH2 (Millipore Sigma, Milwaukee, WI, USA; >95%). For WTWA1 ...
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bioRxiv - Cell Biology 2019Quote: ... (TDP43: 5’-gcaaagccaagaugagccu-3’ and EGFP control: 5’-gcaccaucuucuucaagga-3’; Sigma). Silenced cells were collected by trypsinization ...
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bioRxiv - Cancer Biology 2021Quote: ... 5×10−5 M 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), recombinant murine IL-3 (0.2 ng/mL) ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μM 5-fluoro-2’-deoxyuridine (Sigma Aldrich 200-072-5), penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... 5% glycerol) supplemented with 5 μL Benzonase nuclease (Sigma, E1014-25KU), 1 mM phenylmethanesulfonyl fluoride ...
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bioRxiv - Cell Biology 2022Quote: ... livers were perfused with 5 μg/200 ml ADAMTS-5 (Sigma) for 1h ...
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bioRxiv - Plant Biology 2019Quote: ... a 5 mm agarose (5 g/L, Sigma Aldrich Chemie, CHE) layer was poured into a Petridish (9 cm diameter ...