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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Membranes were then washed with PBST for 5 min and incubated with the following primary antibodies: GAPDH (EMD Millipore, #MAB 374, 1:2000), β-catenin (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked with blocking buffer (TBS containing 0.1% Tween-20 with 5% w/v skim milk) and then incubated with the primary antibody (anti-FLAG M2 (Sigma-Aldrich, USA; #F1804) or anti-alpha-tubulin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Biochemistry 2021Quote: ... 30 KDa for T-protein and L-protein and 100 KDa for P-protein) were purchased from Millipore (Billerica, MA, USA). Mut Express II Fast Mutagenesis kit V2 was purchased from Vazyme (Jiangsu ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then precipitated and resuspended in digestion buffer (100 mM Tris pH 8.5, 1% sodium deoxycholate [Sigma-Aldrich], 5 mM TCEP, and 30 mM CAA). Trypsin and Lys-C proteases were added to a 1:25 and 1:100 ratio (weight/weight) ...
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bioRxiv - Microbiology 2022Quote: ... The SPA-tagged Rho protein was visualized by hybridization with the primary mouse ANTI-FLAG M2 monoclonal antibodies (Sigma-Aldrich; dilution 1:5,000) and the secondary goat peroxidise-coupled anti-mouse IgG antibodies A2304 (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used for immunofluorescence on whole-mounted retinal sections were brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID:AB_94166) at 1:1000 and GFAP (1:1000) ...
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bioRxiv - Cell Biology 2021Quote: ... and screened for expression of the fusion proteins by western blotting with an anti-FLAG M2-HRP antibody (Sigma, mouse monoclonal, cat#: A8592).
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight while shaking at 4°C in the blocking solution that contained combinations of the following primary antibodies: mouse anti- three microtubule-binding domain tau protein (3R-tau, 1:500, Millipore, Cat# 05-803), rabbit anti-purkinje cell protein 4 (PCP4 ...
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bioRxiv - Immunology 2019Quote: ... Chromatin (5 μg) was used for immunoprecipitation reactions on Protein A beads with 0.5 μg of polyclonal antibodies for H3K4me1 (Millipore Sigma Cat. 07-436), H3K4me2 (Millipore Sigma Cat ...
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bioRxiv - Cancer Biology 2019Quote: ... The anti-Phosphoepitope SR proteins (Cat#MABE50; clone 1H4) and anti-SRp40 (Cat#06-1365) antibodies were purchased from Millipore Sigma (Temecula, CA) and used at 1:500 dilution ...
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bioRxiv - Biophysics 2020Quote: ... 20 μL of either eIF4GI or DAP5 capture antibodies were incubated with PureProteome™ Protein A/G mix magnetic Beads (EMD Millipore Corporation) at room temperature with continuous mixing for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... 25 µg of total protein were separated by denaturing PAGE and subjected to Western blotting using mouse monoclonal anti-FLAG antibody (M2, Sigma Aldrich, 1:1000) followed by probing with an HRP-coupled anti-mouse light chain-specific secondary antibody (1:10000 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with 1 µg/ml of a SARS-CoV nucleocapsid protein (NP) monoclonal antibody (mAb) 1C7C7 (Millipore-Sigma, St. Louis, MO) shown to cross-react with SARS-CoV-2 NP ...
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bioRxiv - Neuroscience 2022Quote: ... The immunoprecipitated proteins were analyzed by SDS-PAGE and Western blotting using the following antibodies: anti-HA (Sigma, H3663; clone 12CA5, 1:1000), anti-Flag (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Control conditions included a set of micropattterned grids that were not incubated in antibody (No Ab) and another set of micropatterned grids that were treated with monoclonal antibodies to VZV glyco-protein E (gE; EMD Millipore Cat# MAB8612).
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight while shaking at 4◦C in the blocking solution that contained combinations of the following primary antibodies: mouse anti-3 microtubule-binding domain tau protein (3R-Tau, 1:500, Millipore, Cat# 05-803), rabbit anti-Purkinje cell protein 4 (PCP4 ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Neuroscience 2023Quote: Whole retinas were flat-mounted on slides coated with Gatenby’s solution and stained sequentially using the following primary antibodies: brain-specific homeobox/POU domain protein 3A (Brn3a; Millipore; cat #MAB1585; RRID: AB_94166) at 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... separate sets of sections were immunohistochemically processed using anti-glial fibrillary acidic protein (GFAP) monoclonal antibody 1:500 (G3893, Sigma, Saint Louis, Missouri), anti-doublecortin (DCX ...
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bioRxiv - Physiology 2023Quote: ... We blocked sections in 5% normal goat serum in phosphate-buffered saline with 0.1% Tween 20 (blocking buffer) for 30 min at room temperature (RT) and then incubated in primary antibody for laminin protein (1:500, Sigma catalog no. L93931) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Puromycin incorporated into the proteins were detected in a Western blot using a mouse-origin anti-puromycin monoclonal antibody (12D10, Sigma MABE343, lot # 3484967) and a goat-anti-mouse HRP-conjugated secondary antibody (Abcam ab6789 ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 mL of working solution of 5 volumes of Optiprep solution (Sigma, D1556) and one volume of diluent (150 mM KCl ...
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bioRxiv - Cell Biology 2020Quote: ... Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen; HSS118307 (Sigma); optineurin siRNA (Invitrogen 4392420) ...
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bioRxiv - Microbiology 2019Quote: ... for 5 min and permeabilized for 5 min with ice-cold methanol (Sigma). Cells were then washed with PBS and blocked with 5% horse serum for 30 min at RT ...
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bioRxiv - Physiology 2019Quote: ... The membranes were blocked with 5 % nonfat dry milk or 5 % BSA (Sigma) for 1 h at room temperature and incubated with the antibodies described in the reagents section ...
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bioRxiv - Microbiology 2021Quote: ... with each rNA 5 μg mixed with 5 μg poly(I:C) adjuvant (Sigma) and boosted with the same dose of rNA and poly(I:C ...
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bioRxiv - Microbiology 2019Quote: ... and primers p181 (5’-GCGAAGATAACAGTGACTCTA-3’ and p54 (5’-CGGCTCTGATTAAATTCTGAAG-3’) (Sigma, UK).
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bioRxiv - Microbiology 2021Quote: ... 5′5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, Taufkirchen, Germany) was added until a colour change was observed ...
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bioRxiv - Microbiology 2021Quote: ... stock solutions of hemin (5 mM in 5 mM NaOH; 51280, Sigma-Aldrich) and riboflavin (500 μM in Milli-Q water ...
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bioRxiv - Zoology 2019Quote: ... for 5 min and then blocked in 5% normal goat serum (Sigma-Aldrich) in PBS-T for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... 5×103 cells were collected in a 5% Triton-X-100 (Sigma-Aldrich) in water solution and then prepared for RNA-seq as previously described (Nichterwitz et al. ...
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bioRxiv - Cell Biology 2019Quote: ... sub-confluent cells were incubated for 5 min with 5-fluorouridine (FU) (Sigma). The cells were fixed in pure methanol at −20°C and processed for FU immunocytochemistry ...
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bioRxiv - Microbiology 2022Quote: ... 5 µL of 5 mM nicotinamide 1,N6-ethenoadenine dinucleotide (εNAD, Sigma, N2630) solution was added to each well sample immediately prior to measurements and mixed by pipetting ...
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bioRxiv - Molecular Biology 2023Quote: ... The compounds 5-aza-dC (5-Aza-2’-deoxycytidine, A3656, Sigma, Shanghai, China) and RG108 (N-phthalyl-L-tryptophan ...
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bioRxiv - Plant Biology 2022Quote: Actinomycin D (ActD) and 5-fluorourasil (5-FU) were purchased from Sigma-Aldrich. Tunicamycin (Tm ...
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bioRxiv - Biochemistry 2024Quote: ... DNA probes (5’-GTTATGAGCCCGACGAGCTACCAGGCTGCT-3’) with a 5’-ethylcarbamate amino linker (Sigma-Aldrich) were covalently immobilized on NHS-activated Sepharose 4 Fast Flow (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: – 5-iodo-2’-deoxyuridine (IdU) and 5-Chloro-2′-deoxyuridine (CldU) (Sigma-Aldrich) were dissolved in sterile DMEM at 2.5mM and 200mM respectively and stored at –20°C ...
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bioRxiv - Genetics 2023Quote: ... 5 µl of 5 mM nicotinamide 1,N6-ethenoadenine dinucleotide (εNAD+, Sigma, N2630) solution was added to each well immediately before the beginning of measurements ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 U/ml nucleoside 5-diphosphate kinase (Sigma-Aldrich, cat. Num. N2635) were added ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cell lysates were transferred to a 96-well plate and reactions were initiated by adding 1 mM 5-thiobutyl butyrolactone (TBBL) and 1 mM 5′,5-dithiobis (2-nitrobenzoic acid) (DTNB) (Sigma Aldrich, D8130) in Pon2 activity assay buffer ...
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bioRxiv - Immunology 2019Quote: ... inhibitors on the autophagic flux pathways (3-MA, 5 μM, Sigma-Aldrich; chloroquine, 5 μM, Sigma-Aldrich; bafilomycin, 5 μM, Sigma-Aldrich), and inhibitors on the mTOR pathway (Rapamycin ...
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bioRxiv - Immunology 2019Quote: ... inhibitors on the autophagic flux pathways (3-MA, 5 μM, Sigma-Aldrich; chloroquine, 5 μM, Sigma-Aldrich; bafilomycin, 5 μM, Sigma-Aldrich), and inhibitors on the mTOR pathway (Rapamycin ...
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bioRxiv - Cell Biology 2022Quote: ... Lipids were separated using a solvent mixture of triethylamine/chloroform/ethanol/water (5:5:5:1, v/v) (all solvents HPLC grade, Sigma-Aldrich, USA) as mobile phase in a solvent vapour saturated twin trough chamber (CAMAG ...
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bioRxiv - Cell Biology 2023Quote: ... FAIK3-5 were treated with 1 µM of the hypomethylating agent 5-Azacytidine (5-Aza, Sigma-Aldrich, St. Louis, MO, United States) in an additional approach ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...