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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... then incubated with primary antibodies diluted in 5% milk overnight at 4°C (anti-ATG13 (SAB4200135, 1:100, Sigma, MI, USA); anti-GAPDH (10494-1-AP ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed five times with TBS and incubated with secondary antibody (goat-anti-rabbit/mouse HRP-conjugated,1:2000 in TBS + 5% dry milk, Sigma Aldrich) for 45 minutes at RT ...
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bioRxiv - Genomics 2020Quote: ... 0.14M NaCl and 0.05% TritonX-100) containing a 5-methylcytosine antibody cocktail (5µg; MABE146, SAB2702243; Sigma and NBP2-42813, Novus Biologicals) overnight with tumbling at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were blocked in %5 BSA for 2 h at room temperature and incubated with anti-desmin antibody (1:20; Sigma, D8281) for two overnights ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked for one hour at room temperature (%5 nonfat dried milk in %0,2 TBS-T) and probed with anti-desmin antibody (1:1000; Sigma-Aldrich, D8281) overnight at 4°C or anti-lamin B1 antibody (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... Blocking with 5% BSA in PBS for 1 hour at 37°C and incubation with an anti single-stranded DNA antibody (Sigma, MAB3034) diluted 1:80 in 5% BSA in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with primary antibody (see Reagents) diluted 1:1000 in PBST containing 5% BSA and 0.02% sodium azide (Sigma-Aldrich; S2002) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Tween (TBST) with 10% non-fat dried milk and probed with respective primary antibodies in TBST with 5% BSA (Sigma Aldrich) over night at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and then immunoblotted with the following primary antibodies in TBST with 5% milk at 4°C overnight: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex antibody cocktails were made in a whole-mount blocking solution consisting of 5% heat activated normal donkey serum (Sigma Millipore), 20% Dimethyl sulfoxide (Sigma Millipore) ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% bovine serum albumin and probed with the following primary antibodies: β-actin (Sigma, Cat. No. A1978), EGFR (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... sections were re-blocked in blocking buffer for 1 hour at room temperature then incubated in primary antibody solution containing mouse anti-5-HT1A receptor (Millipore Sigma, cat ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated (2 h, room temperature) with the relevant secondary antibody (see above) and 5 min with DAPI (1:1000, Sigma D817) as a nuclear counterstain ...
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bioRxiv - Neuroscience 2023Quote: ... PLUS and MINUS PLA probes were diluted in the Duolink Antibody diluent in 1:5 ratio with blocking buffer from the PLA kit (Millipore Sigma). The coverslips were then incubated in PLA probe solution for 1 hour at 37°C in humidity chamber ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with homemade anti-RBM20 (1:400) primary antibody in blocking buffer [PBS containing 5% goat serum (cat# G6767; Sigma-Aldrich), 0.1% Triton X-100 (cat# X100 ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour and incubated with the following primary antibodies diluted in 5% milk with TBST overnight at 4°C: Anti-β-actin (1:5000, A5441-.2ML Sigma); Anti-VAPA (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... of each IgG were prepared by using ~ 500 μl of antibody (mAb) (1-5 mg/ml) was mixed with 1200 μl of 100mM Sodium Acetate (sodium acetate anhydrous from Sigma Aldrich) having 1mM Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA for electrophoresis ...
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bioRxiv - Developmental Biology 2023Quote: ... Incubation of the primary antibodies was followed by three 15-minute washes with PBT and subsequent incubation with Alexa Fluor-conjugated secondary antibodies (1:500 in 5%NGS) and DAPI (# D9542; Millipore Sigma) (1:1000 ...
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bioRxiv - Genetics 2023Quote: ... The membrane was blocked with 5% nonfat dry milk in Tris-buffered saline for 1 h and incubated with primary antibodies for HTT (MAB2166, Sigma-Aldrich) for 12 h at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then incubated overnight at 4°C with the primary antibody diluted in blocking solution (5% goat and/or donkey serum in PBS, #G9023 and #D9663 Sigma-Aldrich). Cells were washed with complete PBS 3 times for 5min at RT and then incubated with the following secondary antibodies for 1h at RT ...
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bioRxiv - Genetics 2023Quote: ... The next morning membranes were washed once with blocking buffer for 5-10min and incubated with horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:10 000 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were fixed with ice-cold acetone/methanol (1:1 v/v) and a specific antibody binding was blocked with 5% BSA (w/v, Sigma-Aldrich). Organoids were then incubated with primary antibodies against acetylated α-tubulin (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were washed three times with PBST for 5 minutes each before incubating with anti-mouse secondary antibodies (Sigma-Aldrich, F1804) for at least 60 minutes ...
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bioRxiv - Immunology 2024Quote: ... After 3 x 5 min washes in deionized water unspecific binding sites were blocked with 0.5% fish skin gelatin (FSG) in PBS for 10 min before incubation with gold conjugated mouse IgM antibody (G5652, Sigma Aldrich) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked with 5% BSA at room temperature for 1 hour and incubated with anti-FLAG M2-peroxidase antibody (Sigma-Aldrich) at room temperature for another hour ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was blocked with 5% BSA and incubated with primary antibodies against Tuj-1 (dilution 1:10,000, Sigma, Catalog # T8578) and pan-Ago (dilution 1:1200 ...
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bioRxiv - Genomics 2019Quote: ... About 5×10^5 to 5×10^6 cells were crosslinked with final 1% formaldehyde (Sigma, F8775-500ML) for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... a single pulse of 5-HT consisted of a 5-min application of 50 μM 5-HT (Sigma) to SNs ...
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bioRxiv - Plant Biology 2019Quote: ... pulled-down proteins were separated on SDS-PAGE and analyzed by immunoblotting with anti-Flag and anti-Myc antibodies (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... Protein translation was analyzed on the WES automated western blotting system (ProteinSimple) using anti-Puromycin antibody (1:50; Sigma-Aldrich, MABE343).
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Molecular Biology 2021Quote: ... Following the incubation with a secondary antibody with a linked horse radish peroxidase the proteins were visualized by applying Immobilon™ Chemiluminescent Substrate (Millipore) and detection on an X-ray film.
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bioRxiv - Genetics 2019Quote: Cell extracts were prepared by TCA precipitation and proteins were separated by 10% SDS-PAGE followed by immunoblotting with anti-HA antibody (Sigma-Aldrich, ref ...
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bioRxiv - Cell Biology 2019Quote: ... The lysates were incubated with primary antibodies for overnight at 4°C and then protein A/G magnetic beads (Millipore, USA) were added for 2 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... on genomic DNA isolated from 3-week-old tissue-cultured plants as a template and then selected by northern blot analysis with a HT specific cDNA fragment as probe and subsequently by western blot analysis with antibodies specific to rat HT protein (Sigma-Aldrich).
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Cell Biology 2021Quote: ... The beta subunit of complex V and proteins bound to it were immunoprecipitated using an antibody to the beta subunit of ATP synthase (Millipore, MAB3494) in accordance with the Pierce™ Co-Immunoprecipitation Kit (Cat.No:26149) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were adjusted to 1 mg/ml in lysis buffer and subject to overnight immunoprecipitation at 4°C with either USP7 or USP11 antibody and protein A agarose beads (Sigma-Aldrich), or with GFP-NanoTrap beads (prepared in house ...
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2021Quote: ... and stained with sACE2-humanFc fusion protein (construct is a gift of Jason McLellan, University of Texas at Austin) or mouse anti-Flag antibody (Sigma, F3165). Cells were then stained with anti-human-IgG-FITC (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Puromycin-labeled proteins were detected either by Western blotting or by indirect fluorescence microscopy using a monoclonal anti-puromycin antibody (12D10; Sigma Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000; AC-15, Sigma-Aldrich), nucleolin (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...