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Citations for Millipore Sigma :
1251 - 1300 of 10000+ citations for Protein Phosphatase 1 Catalytic Subunit Gamma Ppp1cc Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were lysed with chilled RIPA buffer (1% Triton X-100, 140mM NaCl, 50mM Hepes, 10mM Idoacetamide, 1×MS-SAFE Protease and Phosphatase Inhibitor (Sigma), PH7.4) ...
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bioRxiv - Microbiology 2019Quote: Quantified protein (between 80-160 μg) was incubated with protein-specific antibodies (either R anti-HA (Sigma) or anti-NS4A ...
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bioRxiv - Molecular Biology 2020Quote: PTP-tagged protein expression was demonstrated by immunoblotting with a rabbit polyclonal anti-protein A antibody (SIGMA) as described previously [13] ...
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bioRxiv - Immunology 2020Quote: ... and ACE2-Fc were serially diluted in diluent buffer (1X PBS, 0.5% bovine serum albumin, 2% filtered fetal bovine serum, 0.2% bovine gamma globulins (Sigma), 5 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... OC antibody of 1:25,000 and A11 antibody of 1:500 (Millipore and Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µM each of leupeptin and E64 protease inhibitor and phosphatase inhibitor cocktail (Sigma, catalog# P5726). For FOXO-1 analysis ...
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bioRxiv - Bioengineering 2021Quote: 1 × 106 cells were lysed in complete RIPA buffer with protease and phosphatase inhibitors (Sigma-Aldrich). Total protein was quantified using the Pierce BCA Protein Assay Kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF) and 1x PhosSTOP phosphatase inhibitor cocktail (Sigma-Aldrich), and centrifuged at 10,000 x g for 10 minutes at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... the plate was incubated with anti-mouse IgG conjugated to alkaline phosphatase (1/4,000; Sigma-Aldrich) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Posterior midguts were dissected into ice-cold PBS with phosphatase inhibitor (1:100, Sigma, cat#P5726). Guts were in ice cold PBS/4% PFA with phosphatase inhibitor and then taken through a methanol (MeOH ...
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bioRxiv - Physiology 2021Quote: ... 1% Triton X-100 and 10% glycerol with freshly added protease and phosphatase inhibitor cocktail (Sigma). The protein concentration was determined by Bradford protein assay (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by the addition of 1M NH4HCO3 (2 mL) and 1 U of alkaline phosphatase (Sigma). The solution was incubated at 37°C for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... pH 8.0) containing 1 mM PMSF and protease and phosphatase inhibitor cocktails (Sigma, # 4906845001 and #11836170001) with three 1.4 mm Zirconium oxide beads (Bertin Instruments ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Immunology 2023Quote: ... The protein antibody used for western blotting was mouse or rabbit anti-6xHis antibodies (Millipore-Sigma). Secondary antibodies were IRDye 680RD goat anti-mouse and/or IRDye 800CW goat anti-rabbit (Li-Cor) ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was used to detect protein expression using the following antibodies on paraffin sections: SftpC (rabbit, Millipore, AB37086, 1:250), and Ki67 (eBioscience ...
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bioRxiv - Microbiology 2020Quote: ... and equal amounts of protein were analyzed using the following antibodies: anti-FLAG M2 (Sigma, cat#F3165, RRID:AB_259529; 1:7,500), anti-IE1 (clone 1B12 (Zhu et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Cell Biology 2019Quote: ... antibodies were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 (GE Healthcare Life Sciences).
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...
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bioRxiv - Plant Biology 2023Quote: ... 5-day- old light- and dark-grown seedling protein extract was incubated with 1 µl anti- phosphoserine antibody (Sigma, USA) with gentle rotation for 2h at 4°C followed by centrifugation and subjecting the pellet to western blotting as described by Matsushita et al ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Cleared cell lysates were immunoprecipitated using 1 μg FLAG-M2 antibody coupled to 40 μL of packed protein G Sepharose beads (Cat GE17-0618-01, Sigma) for 3 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were processed with a free-floating section staining protocol for immunofluorescence using unconjugated primary antibodies (βIV-Tubulin, T7941, Sigma‒Aldrich, dilution 1:400; GFAP, glial fibrillary acidic protein, G-A-5, Sigma‒Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mNG (Vanderbilt Antibody and Protein Resource Core, VU527) (1:1000) or anti-ɑ-tubulin (Sigma-Aldrich Cat# T5168, RRID:AB_477579) (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... CAC-NIH-NA-02)42–44, or anti-zebrafish Ncam1 antibody (1:100, Institute for Protein Innovation) in PBS containing 10 U/mL Heparin (Sigma), 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Plant Biology 2020Quote: ... Four washes of 15 min each in TBST at room temperature and then it was incubated two hours with a secondary antibody anti-rabbit (goat) conjugated with alkaline phosphatase (A3687, Sigma), in a 1:2,500 dilution with TBST ...
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bioRxiv - Bioengineering 2020Quote: ... the plates were incubated with the mouse anti-HA antibody followed by goat anti-mouse IgG-alkaline phosphatase (Sigma-Aldrich). The absorbance at 405 nm was read by the microplate reader (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed multiple times in TBS-T for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; Sigma) as above at 1:2500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Nitrocellulose membrane was washed in TBST for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; catalogue number A3562) (Sigma,) in 5% skim milk TBST at 1:2500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Hybridized probes were detected using alkaline phosphatase-conjugated antibodies (Anti-Digoxigenin-AP, #11093274910, and Anti-Fluorescein-AP, #11426338910, Sigma-Aldrich) and visualized by staining with 4-nitro blue tetrazolium (NBT ...
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bioRxiv - Microbiology 2021Quote: ... Sera were incubated for one hour at room temperature followed by three washes with PBST before incubating with an anti-mouse IgG secondary antibody conjugated to alkaline phosphatase (Sigma) used as a 1:5000 dilution for one hour ...
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bioRxiv - Biophysics 2021Quote: ... A Western blot was performed on aliquots from the supernatant and pellet using monoclonal anti-polyHistidine−alkaline phosphatase antibody (Sigma). Blots were images on a Typhoon FLA 9500 and band intensities were quantified using ImageQuant (Cytiva) ...
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bioRxiv - Immunology 2020Quote: ... then binding was detected with specific alkaline phosphatase-conjugated secondary antibodies (anti-mouse total IgG and anti-monkey total IgG from Sigma and anti-mouse IgG1 and IgG2a from BD Biosciences) ...
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bioRxiv - Neuroscience 2022Quote: ... 1-2 mg protein lysate in 750 µl SLB buffer with protease and phosphatase inhibitors was incubated with 2.5-5 µg of anti-Flag antibody (Sigma, # F3165) for 2 hours at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated overnight with primary antibodies at the following concentrations: chicken anti-Microtubule Associated Protein 2 (MAP2) polyclonal antibody (Sigma-Aldrich, EMD Millipore, Darmstadt, Germany, AB5543, 1:1000) and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β ...
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bioRxiv - Cancer Biology 2021Quote: ... A leukocyte acid phosphatase kit (#387A) for tartrate-resistant acidic phosphatase (TRAP) staining was purchased from Sigma-Aldrich. LipoD293 DNA in vitro transfection reagent (#SL100468 ...
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bioRxiv - Cancer Biology 2022Quote: ... 25mM Tris) supplemented with protease and phosphatase inhibitors (Protease inhibitor cocktail, Phosphatase inhibitor cocktail 2/3, Sigma Aldrich) for 5 minutes ...
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bioRxiv - Physiology 2019Quote: ... Differentiated osteoclasts with >3 nuclei were identified as tartrate-resistant acid phosphatase-positive (Leukocyte Acid Phosphatase Assay, Sigma).
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bioRxiv - Molecular Biology 2022Quote: Antibodies that recognize the following proteins and epitope tags were used: glucose-6-phosphate dehydrogenase (G6PDH; Sigma-Aldrich A-9521, 1:20,000 or 1:50,000), H2B (Active motif 39237 ...
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bioRxiv - Genetics 2021Quote: ... PolyUbiquitinated proteins (1/1000, Sigma 04-262), p62 (1/1000 ...