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Citations for Millipore Sigma :
1051 - 1100 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... HSF5-N2 and control IgG antibodies were crosslinked to 50 µl of protein A-Dynabeads (Thermo-Fisher) by DMP (Sigma). The antibody-crosslinked beads were added to the testis extracts prepared above ...
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bioRxiv - Physiology 2023Quote: ... Lysates were collected by centrifugation and 500 μg of protein were incubated with 0.5 μl of Flag antibody (#F3165, Sigma-Aldrich) at 4 ℃ overnight ...
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ANGPTL8 R59W variant influences inflammation through modulating NF-κB pathway under TNFα stimulationbioRxiv - Biochemistry 2023Quote: ... GAPDH was used as internal control for protein loading and detected using anti-GAPDH antibody (ab2302; Millipore, Temecula, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... Cleared cell lysates were immunoprecipitated using 1 μg FLAG-M2 antibody coupled to 40 μL of packed protein G Sepharose beads (Cat GE17-0618-01, Sigma) for 3 h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Non-antigen specific IgG and RBD-specific IgG were isolated from serum and eluted RBD-specific antibodies respectively by protein G beads (Millipore). Purified IgG was denatured and treated with PNGase enzyme (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mNG (Vanderbilt Antibody and Protein Resource Core, VU527) (1:1000) or anti-ɑ-tubulin (Sigma-Aldrich Cat# T5168, RRID:AB_477579) (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed as previously described7 using Protein G Dynabeads (LifeTechnologies) coupled to Myc 4A6 antibody (Merck Millipore, 05-724) or FLAG M2 monoclonal antibody (Sigma ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... CAC-NIH-NA-02)42–44, or anti-zebrafish Ncam1 antibody (1:100, Institute for Protein Innovation) in PBS containing 10 U/mL Heparin (Sigma), 10% FBS ...
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bioRxiv - Plant Biology 2024Quote: ... Expression of proteins was validated by performing a Western blot analysis against the FLAG-epitope tag using an anti-FLAG antibody (Sigma). For the EMSA assays ...
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bioRxiv - Cell Biology 2024Quote: ... and following centrifugation immunoprecipitations were done using supernatant (I mg protein) and anti-FLAG antibody (Sigma #F1804; dilution 1:1000) and Protein G agarose beads (60 µL ...
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bioRxiv - Cell Biology 2024Quote: ... and screened for expression of the fusion proteins by Western Blotting with an α-FLAG M2-HRP antibody (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of protein was diluted to a concentration of 1 µg/µl and 2 µg of anti-FLAG M2 antibody (Sigma) was added and incubated overnight at 4°C for IP ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Primary antibodies raised against the following proteins were used in block buffer (5% BSA, 0.1% Tween-20, in PBS) at the indicated dilutions: α-Tubulin (Sigma Aldrich T5168, 1:50,000), FLAG (Agrisera AS15 2871 ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µg equivalent of protein from each sample were then treated with 5 mM tris (2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich Cat No: C4706) to reduce disulfide bonds and the resulting free cysteine thiols were alkylated with 10 mM chloroacetamide (Sigma Aldrich Cat No ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 2 mg equivalent of protein from each sample was then reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich Cat No: C4706) for 30 minutes at room temperature and alkylated with 10 mM chloroacetamide (CAA ...
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bioRxiv - Cell Biology 2019Quote: ... 10 µM 5-aza-2′-deoxycytidine (5’-aza-dC; Sigma) was added to the medium ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μL of 5 mM biotin picolylazide (900912 Sigma-Aldrich) and 25 μL of click reaction mix (3 parts TBTA 5 mM TBTA in butanol:DMSO (4:1 ...
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bioRxiv - Neuroscience 2021Quote: ... 30 uM of 5-hydroxytryptamine hydrochloride (5-HT) (Millipore Sigma) was applied into DIV5 cortical neuronal and cortical mixed cultures daily ...
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bioRxiv - Genomics 2020Quote: ... we added 10uM 5-Azacytidine (5-Aza, Sigma-Aldrich #A2385) to induce differentiation ...
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bioRxiv - Cancer Biology 2021Quote: 5-Fluorouracil (5-FU) (F6627, Millipore Sigma, St. Louis, MO) was administered in sterile 0.9% NaCl by intraperitoneal injections (i.p. ...
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bioRxiv - Immunology 2022Quote: ... injection of 150 µg 5-fluorouracil (5-FU, Sigma-Aldrich) per kg of body weight [26 ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM MgCl2 and 5 KU Benzonase nuclease (Merck Millipore) for 30 mins at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-Bromouridine 5′-triphosphate (BrUTP) were purchased from Sigma Aldrich.
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bioRxiv - Biochemistry 2021Quote: ... Bioconjugation of 5-hydroxytryptophan with 5-aminofluorescein (FLA) (Sigma-Aldrich) was carried out as reported [21] with several adjustments ...
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bioRxiv - Neuroscience 2022Quote: ... 5-min pulses of 50 μM 5-HT (Sigma, H9523) with a uniform interstimulus interval (ISI ...
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bioRxiv - Immunology 2023Quote: ... β2-mercaptoethanol (5×10−5 M; Sigma-Aldrich, MO, USA), and 10% heat inactivated foetal calf serum (FCS ...
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bioRxiv - Neuroscience 2024Quote: ... 5 glucose or 5 sodium D-/L-β-hydroxybutyrate (Sigma) or 1.25 sodium lactate (Sigma ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was equally adjusted using Bradford protein assay (Sigma).
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bioRxiv - Synthetic Biology 2020Quote: ... Protein was concentrated in 10,000 MWCO Amicon protein concentrators (Sigma), protein concentration was assayed by Qubit ...
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bioRxiv - Genetics 2020Quote: ... Proteins were quantified by Bradford Protein Assay Kit (Sigma-Aldrich). Protein samples were separated by 4-12% Bis-Tris Protein Gels (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was determined using Bradford protein assay reagent (Sigma), measuring absorbance at a wavelength of 595 nm on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Cell Biology 2019Quote: ... Protein A and Protein G-Sepharose (Sigma-Aldrich, #P3391, #P3296), IgG sepharose 6 Fast Flow (#GE17-0969-01).
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bioRxiv - Biochemistry 2022Quote: ... and the protein stained using ReadyBlue protein stain (Sigma-Aldrich). Fluorescent DNA bands and protein bands were quantified using the Bio-Rad Image Lab software 6.0.1.
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bioRxiv - Molecular Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added to the mix followed by incubation at room temperature for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 5-Fluorouracil (Sigma) was reconstituted in DMSO (100 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... 5% BSA (Sigma) in PBS was injected into the tail vein of mice at 10 µL/g body weight ...
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bioRxiv - Cancer Biology 2019Quote: ... 5% FBS (Sigma), penicillin streptomycin solution (1x ...
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bioRxiv - Neuroscience 2021Quote: ... 5% FBS (Sigma), penicillin ...
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bioRxiv - Neuroscience 2020Quote: ... 5% glucose (Sigma), 0.8 mg/mL glucose oxidase (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5% BSA (Sigma) and 5% donkey serum (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... 5% phenol (Sigma), 5% H2O ...
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bioRxiv - Neuroscience 2019Quote: ... 5% Solutol (Sigma) and 91% normal saline drug vehicle ...
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bioRxiv - Developmental Biology 2019Quote: ... + 5% BSA (Sigma). Primary and secondary antibodies were diluted in PBS 0.05% Tween20 + 5% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... 5% IGEPAL (Sigma) solution was added to reach a final concentration of 0.32% ...
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bioRxiv - Neuroscience 2021Quote: ... 5-HT (Sigma). The concentrations of nutrient solutions were selected to be within the range that is typically ingested ...