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Citations for Millipore Sigma :
1051 - 1100 of 1164 citations for 7 Methylbenz a anthracene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Female C57BL/6 mice wild type mice (7-9 weeks) were injected intraperitoneally with etomoxir (100 µg, Sigma Aldrich, Cat. No E1905) and/or LPS (1 µg ...
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bioRxiv - Neuroscience 2021Quote: ... during 7 days, in a medium composed of equal volumes of modified C2C12 differentiation medium (DMEM, 2% horse serum (Sigma-Aldrich, #H1270),1% antibiotic-antimycotic (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μg peptides were adjusted to pH 7-8 by adding one volume of 50 mM ammonium bicarbonate (ABC, Sigma-Aldrich, A6141). Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Synthetic Biology 2022Quote: ... the reaction mixture consisted of 125 µl of 55 mM phosphate buffer adjusted to pH 7 and 10 µl of the substrate 1 mM ρ-nitrophenyl butyrate (PNPB) (Sigma N9876) homogenised in 2-propanol ...
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bioRxiv - Microbiology 2021Quote: Oral microgavage of 6 dpf larval zebrafish was performed using pH indicators m-cresol purple (0.2%; Thermo-Fisher, 2303-01-7) or phenol red (2%; Sigma, 143-74-8). The fish were then imaged on a Leica MZ10F stereoscope using a Leica MC190 HD color camera ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral supernatants were generated by transfecting the plasmids into 293T cells and used to infect 8988T cells that were then selected for at least 7 days with 2 μg ml−1 puromycin (Sigma-Aldrich, P8833). The RNAi consortium clone IDs for the hairpins used were ...
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bioRxiv - Plant Biology 2021Quote: ... mixed with 0.1 or 0.01% (w/v) CO solution and CNF dispersion in DW at 1% (w/v) in magenta boxes (GA-7; Sigma-Aldrich, USA). Plants were grown in a growth cabinet (BiOTRON ...
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bioRxiv - Biochemistry 2021Quote: ... a biotinylated DNA oligonucleotide complementary to the target tRNA (Supplementary Table 7) was immobilized on Novagen Streptavidin Agarose resin (Merck-Millipore, Burlington, MA) in a 0.22 μm Ultrafree-MC filter cup (Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... the protocol described by Choi et al.65 was applied using home-made wash buffer (50% formamide (Merck Millipore, 75-12-7), 5XSSC (Molecular Biology-P ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: Recombination of the Bbs5flox/flox allele was induced in juvenile CAGG-creERT2 (Jax Mice stock# 004682) positive mice at postnatal day 7 by a single intraperitoneal (IP) injection of 9 mg tamoxifen (Millipore Sigma, T5648) per 40 g body weight ...
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bioRxiv - Immunology 2020Quote: ... Human breast adenocarcinoma cell line (MCF-7) were obtained (ATCC, Manassas, VA, USA) and maintained in DMEM-F12 (Sigma-Aldrich, MO, USA) with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2021Quote: ... COS-7 (hamster, DSMZ Germany, ACC 60), and Rat-1 (rat, ATCC, CRL-2210) cells were cultured in DMEM (Sigma-Aldrich D6429) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Methyl methanesulphonate (MMS) (#129925) (CAS registry number 66-27-3) and carbendazim (#378674) (CAS no. 10605-21-7) were purchased from Sigma-Aldrich (Merck), UK.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 3 and 7 post-drug or vehicle administration were analyzed to quantify urea levels with a urea assay kit (Sigma-Aldrich, MAK006) according to vendor-provided protocol ...
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bioRxiv - Cell Biology 2022Quote: ... refer to the publication from Susnik et al.32 Similar synthesis protocol was adapted for 59 nm SiO2-RhoB NP using a RhoB isothiocyanate (RBITC) dye (CAS 36877-69-7, Sigma-Aldrich, USA). For 119 nm SiO2-Cy5 NP synthesis ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were generated by culturing marrow cells in poly-L-lysine coated culture plate for 7 days with 50 ng/ml M-CSF in RPMI 1640 (Sigma-Aldrich, R8758) supplemented with 20% FBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Microbiology 2023Quote: Nucleic acids were extracted on ice from 5 to 7 technical replicates of each soil sample by adding 0.5 mL phenol:chloroform:isoamyl alcohol (25:24:1) (Sigma-Aldrich, St. Louis, MO, USA) to 0.5 g of soil in a 2 ml Lysing Matrix E tube (MP Biomedicals ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated on a rotator at room temperature for 1 hour with 7 µg/mL final concentration Immunoglobulin G1 (Sigma-Aldrich M5284) and then affinity purified with Protein G Magnetic Beads (Pierce) ...
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µL of 10 mM of D-Val-Leu-Lys 7-amido-4-methylcoumarin (D-VLK-AMC) (in 10 % DMSO/H2O) (Sigma-Aldrich, #V3138) as plasmin substrate (25 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Protoplasts of Exiguobacterium Strain-Molly (EM-P) were generated by growing the cells in 7%DSS-TSB with Lysozyme and Penicillin G (Sigma Aldrich, Germany). Transformation to protoplast was confirmed by a change in cell morphology (Carl Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 1 ml of RPMI + 2% Bovine Serum Albumin (BSA, heat shock fraction, protease free, essentially globulin free, pH 7, Sigma, Catalog #A3059) to a concentration of ∼2 x 108 CFU/ml ...
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bioRxiv - Plant Biology 2023Quote: ... 600 μl aliquots of diluted chromatin were incubated with 7 μl of the rabbit anti-trimethyl-Histone H3 (Lys27) antibody (Millipore, 07-449) in a rotator at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were fixed for 7 minutes in 2% formaldehyde/0.2% glutaraldehyde solution at room temperature and further incubated with X-Gal (Sigma, Cat. no. B4252) solution for one hour at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Neural crest intermediates were defined with 7 days of 12 ng/mL FGF2 and 10 μM of the activin–nodal inhibitor SB431542 (Sigma, cat# S4317) in CDM ...
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bioRxiv - Cell Biology 2023Quote: ... and the remaining pellet was resuspended in lysis buffer (10 mM HEPES pH 7, 1 mM EDTA, 1% NP-40, 500 mM NaCl, Benzonase [Millipore] and PIC) and sonicated at 10-20% amplitude for three times ...
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bioRxiv - Cancer Biology 2023Quote: ... The single-cell suspensions were then seeded in 6-well plates and cultured for 7 to 14 days in the presence of 60 μM 6-thioguanine (Sigma-Aldrich, A4882) [23] ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: Spirodela was grown on half-strength Schenk and Hildebrandt (½ SH, Duchefa Biochemie, #S0225) liquid medium in MagentaTM GA-7 containers (Sigma Aldrich, #V8505), culture dishes (Greiner Bio-One #664160) ...
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bioRxiv - Neuroscience 2024Quote: ... a group of animals was orchidectomized using ketamine (65 mg/kg) and xylazine (7 mg/kg) (Sigma-Aldrich, St. Louis, MO, USA) injected intraperitoneally as anesthetics ...
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bioRxiv - Molecular Biology 2024Quote: ... The following day membranes were washed 3-times with TBST for 7 min and incubated with corresponding solutions of anti-mouse (A9044, Sigma; 1:15,000) or anti-rabbit (A9169 ...
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bioRxiv - Neuroscience 2024Quote: ... embryos from all genotypes (N = 7 per genotype) were collected at 40 hpf and fixed with 4% paraformaldehyde (PFA; Sigma, Gillingham, UK) in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS for 30□min at room temperature and subsequently fixed in 4% paraformaldehyde (PFA, Sigma-Aldrich, St. Louis, MO, USA; 15,812-7) in PBS at 4□°C for 1 hour ...
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bioRxiv - Immunology 2022Quote: Bone marrow cells isolated from femur and tibia of C57BL/6J mice were differentiated into BMDMs for 7 – 8 days by a standard procedure (65) in DMEM (Sigma, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2019Quote: ... was followed by monitoring the conversion of the fluorogenic substrate Acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin (Ac-DEVD-AMC, Sigma-Aldrich, Sintra, Portugal) to 7-amino-4-methylcoumarin (AMC ...
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bioRxiv - Molecular Biology 2019Quote: ... Plates were fixed after up to 7 days of IAA treatment upon initial plating and stained for alkaline phosphatase (AP) activity (Sigma, cat. 86R-1KT) according to the manufacturer’s protocol.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The following primary antibodies were applied for 24 h (or 48 h for anti-cFos antibodies) at 4°C: mouse anti-APC/CC1 Ab-7 (Millipore, OP80, 1:100), chicken anti-mCherry (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated for 30 min with 10 µM 6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) and 1mg/L Hoechst 33342 dye (Sigma Aldrich, United States) diluted in media ...
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bioRxiv - Bioengineering 2021Quote: ... the hydrogel was aliquoted into 125 µL aliquots and each aliquot was mixed with 25 μL of thrombin (7 U/mL, Sigma Aldrich, Cat# T6884). Immediately after mixing ...
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bioRxiv - Biochemistry 2021Quote: ... lysates of African green monkey COS-7 cells (Cell Lines Service, Eppelheim, Germany) and human HEK293T cells (ECACC via Sigma-Aldrich, Poznan, Poland) were analyzed by western blotting ...
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bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... 2.7 mM KCl [Sigma-Aldrich P9541], 19 mM Tris-HCl pH 7.5, and 0.1% v/v Tween 20 [Sigma-Aldrich P7949] in water) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... was used to determine the peak γTuRC fraction (usually Fraction 7; γ-tubulin, GTU-88 mouse antibody, cat. # T6557, Sigma, St. Louis, MO). A 5 μL sample of the peak fraction was then added to glow discharged copper CF400-CU EM grids (cat ...
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bioRxiv - Cell Biology 2022Quote: ... DCs were treated on day 7 of culture for 24 h with either 10−6M 1,25 dihydroxy-cholecalciferol (Sigma-Aldrich, St. Louis, MO), 10−6M 1,25 dihydroxy-cholecalciferol and 100 ng/ml LPS (Escherichia coli ...
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bioRxiv - Microbiology 2020Quote: ... 1,8-Diazabicyclo[5.4.0]undéc-7-ène (DBU), Controlled pore glass (120-200 Mesh, CPG-3000 Å) were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). Fluorescent rhodamine B silica NPs (Si-NPs ...