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Citations for Millipore Sigma :
801 - 850 of 1164 citations for 7 Methylbenz a anthracene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Antibody-bound chromatin was recovered after incubation for ~7 h at 4°C with Magna ChIP Protein A + G Magnetic Beads (16–663, Millipore). Beads were washed twice with Low salt buffer (0.1% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... For inhibitor treatment, SU-DIPGXIIIp* was treated with 120mg/kg PO daily of entrectinib (HY-12678, MedChemExpress, 7% DMSO (Sigma), 10% Tween 80 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... For some experiments the AMPA/kainate glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 μM, Sigma-Aldrich), the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were grown for 48 h in the presence of 7 µM ouabain (Sigma, O3125; CAS:11018-89-6), then diluted into 14 cm-dishes and grown for 2-3 weeks in the presence of ouabain to obtain isolated clones ...
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bioRxiv - Immunology 2021Quote: ... 18.000 – 25.000 cells of each B cell subset were co-cultured with Tfh cells at a 1:1 ratio for 7 days in complete medium with SEB (1.5 μg/ml; Sigma-Aldrich). After co-culture ...
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bioRxiv - Biochemistry 2019Quote: ... 3 μL of the mixture was used in 57 μL assay buffer (25 mM MES, 10 μM Z-Arg-Arg-7-amido-4-methylcoumarin hydrochloride (Sigma), pH 5.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with PI and 2 mM CaCl2 at 7 * 107 cells/mL and digested in 1 mL aliquots by adding 0.6 U MNase (Sigma Aldrich), resuspended in EX50 at 0.6 U/µL88 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The left second gill arch of control and 7-day exposed fish was collected for histological analysis and placed in 4% aqueous formaldehyde (Sigma). The fixation period lasted for 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... parahaemolyticus cells incubated in ASW microcosms at 4°C for 100 days were re-suspended in 50 mM potassium phosphate buffer (pH 7) containing 1 g of 3-mm-sized glass bead (Sigma), vortexed for 25 min ...
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bioRxiv - Microbiology 2019Quote: ... Buffer exchange from glycine-HCl buffer to click reaction buffer (100 mM Na-Phosphate Buffer, pH 7) was performed with Slide-A-Lyzer Dialysis cassettes (Sigma).
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bioRxiv - Neuroscience 2020Quote: ... Spontaneous IPSCs were isolated by blocking AMPA receptor-mediated events with CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 20uM; Sigma-Aldrich). Miniature events were isolated by blocking sodium channels with the addition of tetrodotoxin (1uM ...
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bioRxiv - Neuroscience 2020Quote: ... 80-05-7), and paracetamol (purity >99.0 %, 103-90-2) and corn oil (as a control vehicle) were obtained from Sigma-Aldrich. EDC compounds were dissolved in corn oil in order to obtain the final concentration showed in Table 1.
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bioRxiv - Microbiology 2019Quote: ... The monocytes cells were differentiated into macrophages by addition of 10−7 M phorbol 12-myristate 13 acetate (PMA) (Sigma) for 48 hours before the infection ...
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bioRxiv - Physiology 2021Quote: ... and then stained for 2 hours in 1 mL of M9 containing 150 μM 2’,7’-dichlorofluorescein diacetate (DCFDA, Sigma) while rotating in the dark ...
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bioRxiv - Neuroscience 2019Quote: ... Ipsilateral cortices were homogenized in 8 mL of MCDB131 medium with 0.5% fatty acid-free BSA using a 7-mL loose-fit Dounce tissue grinder (Sigma-Aldrich) with 10 strokes ...
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bioRxiv - Cancer Biology 2021Quote: ... 150 000 cells in 100 μL of flow cytometry buffer were stained with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) for 15 min at 37°C ...
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bioRxiv - Biophysics 2021Quote: COS-7 cells transfected with WT tau alone or with fyn kinase were similarly stained with tau-5 (Sigma-Aldrich) or with tau-9g3 primary antibody (GeneTex Inc. ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to nitrocellulose membrane and probed with primary antibodies against HA (HA-7 mouse monoclonal, 1:2000 dilution, Sigma; or rabbit polyclonal, 1:1000 dilution, Sigma), α-tubulin (mouse monoclonalDM1A ...
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bioRxiv - Physiology 2020Quote: ... HMDC (passage 7-9) were seeded onto plastic 6-well fibronectin-coated (10 μg/ml in PBS, Sigma-Aldrich, UK) culture plates (described below ...
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bioRxiv - Plant Biology 2020Quote: Plants were fumigated for 30 min with 50 μM 2’,7’-dichlorofluorescein diacetate (H2DCFDA, Millipore-Sigma, St. Louis, MO, USA) in a glass container using a nebulizer (Punasi Direct ...
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bioRxiv - Plant Biology 2020Quote: Plants were fumigated for 30 min with 50 μM 2’,7’-dichlorofluorescein diacetate (H2DCFDA, Millipore-Sigma, St. Louis, MO, USA) in a glass container using a nebulizer (Punasi Direct ...
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bioRxiv - Plant Biology 2020Quote: ... 6 to 7 day old light or dark grown seedlings were pre-treated with 50 μM cycloheximide (CHX, Sigma Aldrich) or with 50 μM proteasome inhibitor Bortezomib (Selleckchem) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were treated with xylene (Cas #: 1330-20-7, Greagent) and cover slipped with the DPX Mountant (Lot #: BVBH4393V, Sigma).
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bioRxiv - Microbiology 2021Quote: ... Aminopeptidase activity was assessed by fluorescence assays using the fluorogenic peptide L-leucine-7-amido-4 methylcoumarin hydrochloride (Sigma L2145) as a substrate as previously described (19) ...
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bioRxiv - Neuroscience 2020Quote: ... before being placed in a recording dish that was continuously perfused at 7-9 mL/min with oxygenated Ames bicarbonate solution (Sigma) warmed to 31-34°C ...
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bioRxiv - Microbiology 2019Quote: ... the 2D-gels loaded with 300 µg were fixed in 10% methanol/7% acetic acid for 30 min and overnight stained with Sypro Ruby (Sigma). Two 2D-gels for staining and protein-spot excising (300 µg ...
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bioRxiv - Microbiology 2019Quote: ... The extract was spotted onto the TLC plate as follows: 7 μl of bacterial extract or 5 μl of 20 μM PQS synthetic standard (Sigma). PQS quantity was then determined by exposing resolved TLC plates to 325 nm UV light ...
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The Polycomb group protein MEDEA controls cell proliferation and embryonic patterning in ArabidopsisbioRxiv - Developmental Biology 2020Quote: ... and evaluated by the rate of synergid rupture in ovules mounted in 7% glucose supplemented with 0.1mg/ml of Propidium Iodide (SIGMA, P4170) imaged by a Leica SP5microscope (Argon laser ...
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bioRxiv - Cancer Biology 2020Quote: ... Coverslips were washed three times in excess PBS and were permeabilised in 0.1% (vol/vol) Triton X-100 in PBS for 7 min and blocked in 1% (vol/vol) BSA (Sigma-Aldrich) in PBS for 30 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Mx-cre+ x Metflox/− offspring (5-7 wk) received five injections of 300 μg polyinosinic/polycytidylic acid (pI:pC, Sigma-Aldrich) in PBS i.p ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were irradiated using 8 J/m2 UV and labelled for 7 hours with 20 µM 5-Ethynyl-2’-deoxyuridin (EdU) and 1 µM Floxouridine (Sigma). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were maintained at 37 °C for 7 d to allow colony formation before stained with 0.5% crystal violet (Sigma-Aldrich) in absolute methanol ...
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bioRxiv - Cell Biology 2020Quote: ... using N2B27 2i + Lif medium at 37°C and 7% CO2 in culture flasks coated with 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... astrocytes were generated by shaking confluent mixed-glial cultures at 10-14 days in vitro (DIV) at 180 rpm overnight to remove microglia followed by 7 days of treatment with cytosine arabinoside (Ara-C, Sigma). To generate microglia ...
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bioRxiv - Bioengineering 2022Quote: ... sections were washed in tap water for 2 minutes and counterstained for 7 minutes in Nuclear Fast Red stain (0.1% Nuclear Fast Red [Sigma Aldrich, St Louise ...
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bioRxiv - Immunology 2022Quote: ... Transient expression of human IL-18BP or IL-18BPΔNΔN was achieved using branched polyethyleneimine (PEI) (Mn ∼ 10 000, Cat.:40,872-7, Sigma-Aldrich, Diegem, Belgium) as transfection reagent (67) ...
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bioRxiv - Neuroscience 2022Quote: ... Lysolecithin (LPC)-induced demyelination experiments were carried out in cerebellar slices from P11 animals at day 7 in vitro by incubation for 16 h with 0.5 mg/ml LPC (Sigma-Aldrich) (Birgbauer et al. ...
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bioRxiv - Neuroscience 2022Quote: Headless larvae (7 dpf) were fixed for three days at 4 °C in EM fixation buffer [2% EM-grade glutaraldehyde (Sigma), 2% PFA (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... All primary cells were used between subculture 3–7 and were routinely cryopreserved in 90% human serum (#H4522; Sigma-Aldrich) supplemented with 10% dimethyl sulfoxide (DMSO).
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bioRxiv - Cell Biology 2022Quote: ... CAS#96736-11-7) was used at a final concentration of 100 μg/ml and thiabendazole (TBZ) (Sigma-Aldrich; T8904) at 5-15 μg/ml in YE media where appropriate ...
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bioRxiv - Neuroscience 2020Quote: ... was transfected into COS-7 cells cultured in a well of 8 well chamber slide glass using GeneJuice Transfection Reagent (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... 50 −100 μg of protein were loaded on a 7%-12% polyacrylamide gel and then transferred on a methanol activated PVDF membrane (Millipore). Blot membranes were blocked for one hr with 5% milk or 5% BSA in TBS with 0.1% Tween 20 (TBST) ...
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bioRxiv - Developmental Biology 2020Quote: ... CAGG-creERT2 mice at postnatal day 7 by a single intraperitoneal (IP) injection of 9 mg tamoxifen (Millipore Sigma, T5648) per 40 g body weight ...
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bioRxiv - Microbiology 2021Quote: ... The mice were euthanized at 7 days post-infection (dpi) and the small intestine was removed and stored in RNAlater (Sigma) at −80 °C for further analysis.
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bioRxiv - Microbiology 2021Quote: ... Cells were seeded at 7 × 105 cells/mL and RNAi induction was initiated with the addition of 1 µg/mL tetracycline (Sigma) and 1 × 107 cells were isolated every 24 hours for RNA analysis (outlined below ...
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bioRxiv - Plant Biology 2021Quote: The glass fibre filter papers (GFFPs) used to collect each sample were submerged in 7-mL volumes of HPLC-grade methanol (>99.9%, Millipore-Sigma Canada) in 20-mL scintillation vials (Chromatographic Specialties Inc. ...
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bioRxiv - Microbiology 2020Quote: ... of resuspended bacterial cells were divided into each of nine acid-washed (1 L) glass bottles containing 700 mL artificial seawater (7 practical salinity units, prepared from Sea Salts; Sigma). The different volumes were to start the experiment with a similar bacterial biomass ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were discarded and cell pellets were washed by adding 1 volume of sterile artificial Baltic seawater (7 PSU, prepared from Sea Salts; Sigma). Cells were resuspended in artificial Baltic seawater and the procedure was repeated twice more.
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bioRxiv - Physiology 2021Quote: ... IF n = 7 pups per sex from N = 7 litters) under Inactin anaesthesia (sodium thiobutabarbital 100 mg/kg body weight i.p., T133, Sigma Aldrich), as described previously [17] ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated with 15 µM FM1-43 for 90 s at room temperature and unbound dye removed by 10 min wash in PBS /1mM scavenger dye ADVASEP-7 (Sigma). Cells were re-incubated with depolarizing solution for 90 s at room temperature (Gaffield et al. ...