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Citations for Millipore Sigma :
751 - 800 of 1164 citations for 7 Methylbenz a anthracene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: Total RNA from 7 DAP spikes was extracted using TRI Reagent and 1-bromo-3-chloropropane (BCP) (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Dead cells and red blood cells were excluded using 1 μg/ml 7-amino actinomycin D (7AAD; Sigma-Aldrich), Fixable Viability Dye eFluor® 780 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Each piece of sclera was rinsed with PBS and then placed into 20 mL of pH 7.5-8.0 extraction medium containing: >1000 BAEE unit/mL trypsin (9002-07-7, Sigma-Aldrich), 4 mM ethylenediaminetraacetic acid (60-00-4 ...
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bioRxiv - Bioengineering 2019Quote: ... or 48-well plates (with or without 7×7mm glass coverslips) pretreated with 50 µg/mL human fibronectin (Millipore).
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bioRxiv - Immunology 2019Quote: ... Cells were incubated for 7 minutes on ice in Red Blood Cell Lysing Buffer Hybri-Max™ (Sigma-Aldrich) for the removal of contaminating erythrocytes ...
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bioRxiv - Cell Biology 2019Quote: ... chopped thoroughly and resuspended in 10 ml digestion solution containing 7 ml Hanks’ Balanced Salt Solution (HBSS, H9269, Sigma), 0.23 g bovine serum albumin (BSA ...
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bioRxiv - Genetics 2020Quote: ... and ~7 μl of each dilution was spotted on SD-Ura or SD plates containing 5-FOA (Sigma Aldrich). Plates were incubated at indicated temperatures for 2-4 days.
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-HA antibody (3F10; Roche Diagnostics, Mannheim, Germany or HA-7; Sigma, Saint Louis, MI, USA), anti-DDX6 (A300-460A ...
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bioRxiv - Immunology 2021Quote: 7-8 week-old B6J females were treated for 10 consecutive days with ampicillin (1 g/L; A0166 Sigma), vancomycin (500 mg/L ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stimulated with 50 μL of 10−7 M phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) or N-formyl-methionyl-leucyl-phenylalanine (fMLP ...
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bioRxiv - Cell Biology 2021Quote: ... Fifteen µg of cell lysate from each hESC line was incubated in 150 µl acetate buffer (pH 4.0) containing a final concentration of 62.5 µM Ala-Ala-Phe-7-amido-4-methylcoumarin (AAF-AMC) (Sigma #A3401) for 20 h at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: TPP1 enzyme activity was measured fluorometrically based on fluorescence of the chromophore 7-amino-4-methylcoumarin (AMC) (Sigma #A9891) as described previously (Lojewski et al. ...
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bioRxiv - Cell Biology 2021Quote: 7-8 week-old age and sex-matched mice were used for treatments with arsenic (5ug/g, Sigma, #202673), pI:C (20 ug/g ...
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bioRxiv - Biochemistry 2022Quote: Three-day-old germinated seedlings were sprayed with a 0.5% pH 7 solution of phosphite (Sigma, St Louis, USA) using a hand-triggered spray bottle and untreated seedlings were sprayed with water 31–33 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transferred to a 5-7 µl drop of CZB covered with mineral oil (Sigma Aldrich, Cat# M5310). A glass cover slip was placed on top of the media drop and pressed down on the edges to spread the media to cover the entire surface of the cover slip ...
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bioRxiv - Molecular Biology 2022Quote: ... Three-mL liquid cultures were inoculated with a single colony and supplemented with 2′,7′-dichlorodihydrofluorescein diacetate (DCFHDA; Sigma) with a final concentration of 1 μM ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25 mM NaH2PO4, 2.5 mM KCl, 0.5 mM CaCl2, 7 mM MgCl2, 25 mM glucose; all from Sigma-Aldrich) saturated with carbogen ...
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bioRxiv - Neuroscience 2023Quote: ... or high pH medium supplemented with 0,01 μM 7-alpha,25-dihydroxycholesterol (7α,25OHC) (Sigma Aldrich, 64907-22-8) was added to the bottom of the chamber ...
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bioRxiv - Neuroscience 2022Quote: ... SDS lysate was centrifuged at 20,000 x g for 30 minutes and supernatants were subsequently diluted with 7 volumes of 1.15% Triton-X 100 in TNE with Phosphatase Inhibitor Cocktail 1 and 2 (Sigma) to produce a mixed-micelle lysate with final detergent concentrations of 0.125% SDS and 1% Triton-X 100 ...
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bioRxiv - Immunology 2023Quote: ... Mice were sacrificed at day 7 (X31) and day 4 (RSV) post infection (p.i.) and lung lobes were stored in RNA-later (Sigma). Lung tissue was homogenized using a TissueLyser LT (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... indole and 7-cyanoindole solutions were prepared at 10 mg/ml in vehicle (10% DMSO and Millipore-filtered water), aliquoted and stored at −20 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Merck; 1 mM CaCl2, Sigma; 2 mM MgCl2, Merck; adjusted to pH 7 with HCl; 0.1% Tween 20, Sigma) overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... At 5-7 days post infection mice were orally gavaged with 200 µl 2mg/ml loperamide hydrochloride (Sigma Aldrich) to minimize peristaltic movement for imaging ...
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bioRxiv - Cell Biology 2023Quote: ... Dried lipids were resuspended in 15µL chloroform and 65µL of click mix containing 400µM 3-Azido-7-hydroxycoumarin (Baseclick #BCFA-047-1) and 900µM tetrakis(acetonitrile)copper(I) tetrafluoroborate (Sigma #677892) in acetonitrile/ethanol (7:3 ...
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bioRxiv - Genetics 2023Quote: ... 30 hours post-fertilization old embryos were exposed to 20 μM 2′,7′-dichlorofluorescein diacetate (DCFH-DA, Sigma-Aldrich) for 1 hour at 28.5 °C in the dark followed by washing with embryo water a minimum of three times 31 ...
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bioRxiv - Biophysics 2023Quote: ... Glyceryl trioleate was either >99% purity (T-235, NuChek Prep) or 97% purity (CAS: 122-32-7, Sigma Aldrich). When dissolving PL within the TAG prior to creating the caps ...
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bioRxiv - Microbiology 2023Quote: ... indole and 7-cyanoindole solutions were prepared at 5 mg/ml in vehicle (10% DMSO and Millipore-filtered water), aliquoted and stored at −20 °C ...
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bioRxiv - Immunology 2023Quote: ROS production in BMDMs was measured using the membrane-permeable fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFHDA) (Sigma, #D6883). BMDMs were plated in 96-well plates at 1×105 cells/well ...
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bioRxiv - Cancer Biology 2023Quote: RNA fractionation of MDA-T32 and MCF-7 cells was performed using the Nuclei EZ lysis buffer (Sigma-Aldrich) as previously described.48 RNA was isolated from the nuclear and cytoplasmic cell fractions using RNA Extracol and GeneMATRIX Universal RNA Purification Kit (EURx) ...
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bioRxiv - Biochemistry 2023Quote: ... Muse® cell analyzer kits for measurement of caspase3/7 (MCH100108) and cell cycle (MCH100106) were from EMD Millipore. Unless otherwise noted ...
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bioRxiv - Cancer Biology 2023Quote: Five hundred A673 or SKES1 cells transduced with shRNAs targeting KCNN1 or shRNA non-targeting control were seeded in 6-well plates after 72 hours or 7 days of doxycycline treatment (0,5 µg/mL, Sigma). Cells were then incubated for 8 to 10 days under standard conditions (humidified incubator at 37°C and 5% CO2) ...
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bioRxiv - Pathology 2023Quote: Human or mouse lung tissue slides (7 μm) were fixed in 3.7% formalin (Sigma-Aldrich, St. Louis, MA, USA), permeabilized in 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a N2B27 with B27 + retinoic acid (with 10 µM DAPT for first 7 days) on PDL (Sigma)/Laminin (Roch ...
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bioRxiv - Physiology 2023Quote: ... and with a rat monoclonal anti-Septin 7 (1:100 dilution, clone 19A4, MABT 1557, Millipore Sigma, Burlington, MA) in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then permeabilized for 7 min with ice-cold PBS supplemented with 0.5% Triton X-100 (Sigma-Aldrich). Cells were blocked in 1X PBS/1% BSA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... 7 mL of collagen solution was mixed with 800 μL Phenol Red (Sigma-Aldrich, ACS Cas.# 143-74-8) to act as pH indicator ...
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bioRxiv - Microbiology 2023Quote: ... Once the cultures reached OD 0.1 then 108 CFU/ml of each strain was incubated with 10µM of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Sigma) in 1xPBS (pH 7.2 ...
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bioRxiv - Bioengineering 2023Quote: ... Pooled fractions (7-10) and (11-30) were then concentrated using 10 kDa cutoff filters (Amicon Ultra-15, Millipore). Each fraction was subjected to protein concentration measurement ...
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bioRxiv - Bioengineering 2023Quote: ... Pooled fractions (7-10) and (11-30) were then concentrated using 10 kDa cutoff filters (Amicon Ultra-15, Millipore). Each fraction was subjected to protein concentration measurement ...
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bioRxiv - Genomics 2023Quote: pSM44 mES cells were grown at 37C under 7% CO2 on plates coated with 0.2% gelatin (Sigma, G1393-100ML) and 1.75 mg/mL laminin (Life Technologies Corporation ...
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bioRxiv - Microbiology 2023Quote: ... promastigotes were incubated for 7 days to maximize infective metacyclics (i.e., those isolated through a step Percoll gradient; Sigma). Amastigotes typically were grown axenically for 5 days prior to use [17] ...
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bioRxiv - Molecular Biology 2023Quote: ... At 7 weeks of age female animals were injected IP with 200mg/kg of tamoxifen (Millipore Sigma, Burlington, MA) dissolved in corn oil for two consecutive days.
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bioRxiv - Cell Biology 2024Quote: COS-7 (ATTC) cells were grown on #1.5 glass coverslips in DMEM containing 10% fetal bovine serum (Sigma-Aldrich), washed twice with 2 ml of 100 mM sodium phosphate and fixed for 2 h in 2 ml PLP (2% [wt/vol] paraformaldehyde in 87.5 mM lysine ...
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bioRxiv - Cancer Biology 2024Quote: ... The cortex was then dissected and dissociated using a papain solution (0.5mM of EDTA, 1mM L-Cysteine and 0.4mg/mL of papain (7 units of papain/mL) (Sigma, #76216)) at 37°C in a water bath for 25 min ...
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ITPK1 is an InsP6/ADP phosphotransferase that controls systemic phosphate homeostasis in ArabidopsisbioRxiv - Plant Biology 2020Quote: Seedlings were grown vertically on half-strength MS medium supplemented with 1 % sucrose and 7 g L−1 Phytagel (P8169, Sigma), pH 5.7 for 12 days (8 h light at 22°C ...
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bioRxiv - Cancer Biology 2021Quote: ... fresh or frozen tissue was quickly minced with Noyes scissors and transferred to a 7 mL glass Dounce homogenizer (Sigma), precooled on ice ...
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bioRxiv - Cell Biology 2019Quote: ... CYP1A2 and CYP2A6 activities were measured by incubating cultures with 5 μM 7-ethoxyresorufin or 50 μM coumarin (Sigma-Aldrich) for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in OM with or without 10μM SP600125 for 7 or 14 days and tested by ARS staining (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... accumulation was assessed by measuring the levels of the oxidized form of the cell-permeant 5-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) (Sigma). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... T cells were then infected with HIVBaL and cultured for up to 7 days in RPMI medium additionally supplemented with 10 U/ml recombinant interleukin-2 (Sigma). The dividing cell lines HEK293T (ATCC ...