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Citations for Millipore Sigma :
851 - 900 of 1164 citations for 7 Methylbenz a anthracene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Parasites were incubtaed in 96 well plates with riboflavin analogues (compounds 1-6 were obtained from the the National Cancer Institute, DCTD/DTP/DSCB, Rockville, MD, USA, and compound 7 and 8 were purchased from Sigma). All compounds were prepared in DMSO except compound 2 which was dissolved in a mixture of DMSO and 1N KOH ...
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bioRxiv - Neuroscience 2022Quote: ... mice were given ad libitum access to 5 mg of clozapine-N-oxide (CNO; Sigma-Aldrich cat. #34233-69-7) dissolved in 200 mL of drinking water.
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bioRxiv - Neuroscience 2022Quote: ... thin sections (70 nm) from resin blocks were cut and stained with Uranyless solution (ProSciTech) for 7 minutes followed by lead citrate (Sigma) for 7 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... NGM medium containing 10 mM methionine or 50 mM choline was made by directly adding 1.5 g L-methionine (BioDee Biotechnology) or 7 g choline chloride (Sigma-Aldrich) respectively into 1 L sterile NGM before pouring plates ...
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Molecular Biology 2022Quote: ... For Huh7-dCas9 knockdown in 12-well dishes 30 µL of each gRNA was combined with 7 µL TransIT-CRISPR transfection reagent (Sigma) in 363 µL OPTI-MEM (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... 5, 6 and 7 (days 52 to 55 in the overall timeline, Figure 2) at 100 mg/kg ip (Sigma), at approximately 17:00 hr ...
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bioRxiv - Biochemistry 2019Quote: The custom designed CompoZr® Zinc Finger Nuclease Plasmid targeted to exon 3 of the mouse ECH1 gene on chromosome 7 was obtained from Sigma. In-vitro transcription of the ZFN mRNA was performed following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... 0.01 g/l CaCl2 ×2H2O) were supplemented with 5 g/l Peptone N-Z-Soy BL 7 (Sigma-Aldrich S.r.l) (complex medium ...
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bioRxiv - Cancer Biology 2020Quote: ... at pH 7 and re-suspended in HBSS with 250μM HEPES and 1% bovine serum albumin (BSA) (Sigma-Aldrich, USA). The cells were sorted based on GFP intensity using “Moflo Astrios EQ” (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... An o-Phenanthroline solution was prepared mixing 30 μL of hydrochloric acid (37%) with 7 mL MilliQ water and dissolving 0.2 grams 1,10-Phenanthroline monohydrate (Sigma-Aldrich, 99%) in it ...
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bioRxiv - Immunology 2020Quote: ... 7-to 8-week old WT mice were injected intraperitoneally with 20 mg/kg body wight of LPS (Sigma, L2630). The mice were administered intraperitoneally 200 μl of DPBS containing no antibody (n = 17) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cathepsin B was measured with the fluorogenic substrate Na-CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin hydrochloride (Sigma) after a 30-min pretreatment with DTT and EDTA ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose was removed by 7 sequential washes in a centrifugal filtration unit with a molecular weight cut-off (MWCO) of 100 kDa (Millipore). Polysomes were converted into monosomes by RNase H digestion ...
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bioRxiv - Pathology 2021Quote: ... the 5th injection of ketamine or SAL included a co-injection of L-DOPA (7 mg/kg, i.p., Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... and the lysate/antibody/bead mix was incubated at 4°C overnight with continuous rotation Beads were then washed in HEPES buffer and boiled in Laemmli loading buffer prior to SDS PAGE and western blot analysis with an anti-HA antibody (HA-7; H9658, Sigma).
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed (20 mM Tris, 500 mM NaCl, 10 mM imidazole, pH 7, protease inhibitor, lysozyme, 250 units of Benzonase (Sigma)) and incubated on ice for 20 min ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Developmental Biology 2019Quote: ... cells were cultured from day 7 in induction medium supplemented with either 1mg/mL Heparin Sodium Salt (Heparin, Sigma-Aldrich), 0.25uM Valproic Acid (VA ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Cathepsin B was measured with the fluorogenic substrate Na-CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin hydrochloride (Sigma) after a 30-min pretreatment with DTT and EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... iPSCs were differentiated into iPSC-CMs using the Wnt modulation-differentiation method (Lian et al., 2012) with 7 μM CHIR (Selleckchem) and 5 μM IWP2 (Sigma) in RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then washed twice with PBS and treated for 7 minutes in freshly-prepared fluorescence quenching buffer (0.1% sodium borohydride (452882-25G, Sigma-Aldrich) in PBS) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 7-dichloro-8-hydroxyquinoline (CLCQ; D64600-Lot#STBH7389) and Zinc Chloride (ZnCl2; 208086-Lot#MKCL1763) were purchased from Sigma Aldrich. We prepared 10mM stocks solutions of the inhibitors in Dimethyl sulfoxide (DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... 10-15 days old astrocytic cultures were subject to a sublethal dose of H2O2 (0.7-1.5 mM) for 45 minutes and subsequently incubated in absence or presence of 500 uM L-carnitine (Sigma-Tau) for 2 hours ...
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bioRxiv - Immunology 2020Quote: Each peptide sample was desalted on Empore SPE C18 cartridges (standard density, bed inner diameter 7 mm, volume 3 ml; Sigma), then concentrated by vacuum centrifugation and reconstituted in 40 µl 0.1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes were resuspended at 1×10^7 cells/mL in complete DMEM and incubated in the presence of 0.1 µM dexamethasone (Sigma-Aldrich) at 37 °C for 18 h ...
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bioRxiv - Neuroscience 2021Quote: ... GTS1-3 tissue was mechanically dissociated with a scalpel in ice-cold GKN-BSA containing 11.1 mM D-(+)-Glucose monohydrate (14431-43-7, Sigma Aldrich) and 50 mM bovine serum albumin (BSA ...
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Systemic administration of Ivabradine, an HCN channel inhibitor, blocks spontaneous absence seizuresbioRxiv - Neuroscience 2021Quote: ... and ELA (N-[4-[2-(3,4-Dihydro-6,7-dimethoxy-2(1H)-isoquinolinyl)ethyl]phenyl]-9,10-dihydro-5-methoxy-9-oxo-4-acridinecarboxamide) were purchased from Sigma-Aldrich. For systemic injections ...
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved MSP1E3D1 was collected in the flow-through fraction and concentrated to 7 mg mL−1 in a 10 kDa MWCO centrifugation spin filter (Millipore).
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bioRxiv - Cell Biology 2022Quote: ... and differentiated at 4×106 cells per 40 mm dish for 7 days at 37 °C in RPMI media (Sigma) supplemented with 1 mM Sodium Pyruvate (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 100 mg fragments were incubated in a 12-well plate with M199 media ± 7 nM insulin (Actrapid, Novo Nordisk) and 25 nM dexamethasone (D4902, Sigma). After 24 hr incubation ...
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bioRxiv - Immunology 2022Quote: ... Plates were incubated at 37 degrees C in 5% CO2 for 7 days before fixing with 2% formaldehyde and staining with 0.1% crystal violet (Sigma-Aldrich).
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bioRxiv - Genetics 2022Quote: For Masson’s trichrome staining, frozen muscle (diaphragm, 7 mice/group) sections were stained in Weigert’s iron haematoxylin working solution (Sigma-Aldrich), Biebrich Scarlet-Acid fuchsin solution (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... starting day 7 post-transfection to select for the loss of Hprt and with 1 μM ganciclovir (GCV, Sigma PHR1593) to select against the LP plasmid backbone that contained a HSV1-ΔTK expression cassette ...
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bioRxiv - Molecular Biology 2022Quote: Estrogen and progesterone receptor-positive breast adenocarcinoma MCF-7 cell line (#HTB-22, ATCC) was cultivated in Dulbecco’s Minimum Essential Medium (DMEM, #D6429, Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... plants were fumigated for 30 min with 50 µM 2′,7′-dichlorofluorescein diacetate (H2DCFDA; Millipore-Sigma, St. Louis, MO, USA) for ROS imaging ...
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bioRxiv - Plant Biology 2023Quote: ... plants were fumigated for 30 min with 50 µM 2′,7′-dichlorofluorescein diacetate (H2DCFDA; Millipore-Sigma, St. Louis, MO, USA) for ROS imaging ...
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bioRxiv - Plant Biology 2022Quote: ... Sections of 7 to 8 mm were then transferred to slides treated with 100 mg/mL poly-L-Lys (Sigma) and dried on a warm plate at 40 °C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... pH of the nutrients medium was adjusted to 7 with NaOH and jelled with 0.7% (w/v) Agar (Sigma-Aldrich). In this study ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Cancer Biology 2022Quote: For live cell imaging cells were seeded on glass bottom plates which were previously coated with either 1mg/ml fibronectin (CKPC cells) or 10 μg/ml laminin (COS-7 cells, #L2020, Sigma). The cells were imaged using a Zeiss 880 LSM with Airyscan microscope which has a 37°C humidified incubator and perfused with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Microbiology 2023Quote: ... The tPA fluorogenic substrate D-Val-Leu-Lys 7-amido-4-methylcoumarin (10 μM) (Millipore-Sigma, Burlington, MA, USA, #A8171) was added ...
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bioRxiv - Microbiology 2023Quote: ... The tPA fluorogenic substrate D-Val-Leu-Lys 7-amido-4-methylcoumarin (10 μM) (Millipore-Sigma, Burlington, MA, USA, #A8171) was added ...
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bioRxiv - Biochemistry 2023Quote: ... male and female Il10−/− mice (n = 7 per sex) were switched to a diet containing 100 ppm of piroxicam (Sigma) (Envigo ...
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bioRxiv - Immunology 2023Quote: Tumors were digested for 45 minutes with gentle shaking at 37°C in HBSS containing 7 mg/ml Collagenase D and 2 mg/ml DNase I (Sigma). Single-cell suspensions were stained with fluorophore-conjugated antibodies obtained from BioLegend ...
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bioRxiv - Bioengineering 2023Quote: Evaluation of fluid velocity of static and flow-conditioned vessels was assessed at day 7 through perfusion of 2.0 µm fluorescent beads (Fluorescent blue latex beads, Sigma Aldrich) into the microvascular networks using reservoirs with applied pressure gradients of 5 mm H2O ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The mineral suspensions were mixed with 0-200 ng/μL salmon sperm DNA in triplicates (also at pH 7) (Sigma). The mineral-DNA suspensions were incubated at room temperature for 16 hrs under constant shaking at 70 rpm ...