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Citations for Millipore Sigma :
1001 - 1050 of 7146 citations for Recombinant Human Interleukin 6 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the receptors were crosslinked by the addition of anti-mouse or anti-rat IgG Alexa Fluor (Sigma) during 15 min on ice.
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bioRxiv - Neuroscience 2021Quote: ... we now infused .22ul of either saline or 2mM muscimol (a GABA-A receptor agonist; Sigma Aldrich). Our infusion targets differed by experiment ...
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bioRxiv - Biophysics 2021Quote: ... The receptor was concentrated to 20–30 mg/ml with a 100 kDa cut-off concentrator (Millipore). Protein purity and mono-dispersity were examined by Nu-PAGE and analytical size-exclusion chromatography.
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bioRxiv - Neuroscience 2021Quote: Primary antibodies and dilutions were: Anti-Purinergic Receptor P2RY14 rabbit (extracellular) (1:200, Sigma Aldrich, Cat#: P0119); GPR105 Polyclonal antibody rabbit (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with an antibody against the M2 muscarinic acetylcholine receptor (1:500 in PBS, MAB367, Millipore) for at least 24 hours ...
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bioRxiv - Immunology 2022Quote: ... sera were heat-inactivated at 56 °C for 30 minutes and treated with receptor destroying enzyme (Sigma) in a 1:4 mixture (5x dilution of sera) ...
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bioRxiv - Neuroscience 2022Quote: ... AMPA-receptor-mediated excitatory postsynaptic currents (EPSCs) were recorded in presence of picrotoxin (100 μM, Sigma Aldrich) to block GABAARs ...
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bioRxiv - Bioengineering 2023Quote: ... The stimulation of ACh to muscarinic receptors was blocked by addition of 10 nM Atropine (Sigma Aldrich). In the presence of Atropine ...
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bioRxiv - Neuroscience 2024Quote: ... 2) GluR2 to visualize postsynaptic receptors (mouse anti-GluR2 at 1:2000, Millipore, catalog # MAB397, RRID: AB_2113875) and 3 ...
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bioRxiv - Neuroscience 2024Quote: ... Aladdin) or β receptor agonist isoprenaline (2 mg/mL, 4 mg/mL, n = 7, I5627, Sigma-Aldrich) or β receptor antagonist propranolol (2·5 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Physiology 2021Quote: ... The neurotoxin (6-hydroxydopamine) was purchased from Sigma-Aldrich (6-OHDA, H4381, St. Louis, MO).
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bioRxiv - Zoology 2019Quote: ... and pterin-6-carboxylic acid (pterin-6-COOH) were purchased from Sigma Aldrich (Munich, Germany). Isoxanthopterin and xanthopterin were obtained from Fluka (Buchs ...
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bioRxiv - Genomics 2021Quote: ... Antigen retrieval was done by microwaving for 6 minutes in citrate buffer pH 6 (Sigma). After quenching in 3% H2O2 in Methanol ...
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bioRxiv - Neuroscience 2022Quote: ... and a solution of 10 mg/mL 6-hydroxydopamine (6-OHDA; Sigma-Aldrich, H116-5MG), dissolved in saline buffer was injected ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were treated with 6-hydroxydopamine hydrochloride (6-OHDA, 15 µM, H4381, Sigma Aldrich) and METTL3/14 activators (100 nM or 1 µM) ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Cell Biology 2019Quote: Immunoprecipitations of Flag-tagged proteins were performed using α-Flag M2 affinity beads (Sigma-Aldrich). For each immunoprecipitation reaction ...
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bioRxiv - Cell Biology 2019Quote: ... Washed cells were treated with TRITC tagged Concanavalin A (20 µg ml-1) (Sigma, USA) for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Cancer Biology 2021Quote: ... All FLAG-tagged proteins were purified with anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) in a native condition using the FLAG peptide elution ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma, #A2220). For the IP ...
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bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).
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bioRxiv - Physiology 2021Quote: ... followed by incubation with fluorescent-tagged carboxylate-coated 2 um size latex beads (L3030, Sigma) for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-tagged Pros-S-HD in soluble fraction was purified using Glutathione-Agarose beads (Sigma). The bound proteins were eluted in elution buffer (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Genetics 2022Quote: V5H6-tagged FRQ was immunoprecipitated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog #7345) from 2 mg of centrifugation-cleared lysate ...
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bioRxiv - Microbiology 2022Quote: ... and FLAG tagged S-protein was stained using anti-FLAG (clone M2, Sigma-Aldrich, F1804). SARS-CoV-2 Spike Antibody (ProSci ...