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Citations for Millipore Sigma :
1051 - 1100 of 7146 citations for Recombinant Human Interleukin 6 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Molecular Biology 2023Quote: ... myc-tagged TEV protease was detected with a mouse monoclonal anti-myc antibody (Sigma M4439). The proteins were detected using SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Flag-tagged Scube was detected by using polyclonal α-FLAG antibodies (Sigma, St. Louis, USA). GAPDH ...
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bioRxiv - Immunology 2023Quote: ... Co-IP of FLAG-tagged proteins was performed by incubating M2 anti-FLAG resin (Sigma) with clarified cell lysates for 2 h at 4°C with end-over-end rotation ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD3 and PFD5 were detected using anti-FLAG rabbit antibody (1:5,000, Sigma), FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% ascorbic acid was added to a sterile saline solution (NaCl, 0.9% w/v) and 6-OHDA HCl powder (6-hydroxydopamine hydrochloride from Sigma or 6-OHDA hydrobromide from Tocris) was then dissolved to produce a final 6-OHDA concentration of 4.44 mg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant GST-p50 subunit D434-969 was purchased from Sigma-Aldrich. Binding reactions were performed for 20 minutes at room temperature with 20 pmol annealed primers ...
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bioRxiv - Immunology 2021Quote: Recombinant cytokines were purchased from indicated suppliers: IFN-β1a (Sigma, #IF014); IFN-γ (Sigma ...
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bioRxiv - Genomics 2020Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... Following 12 h recombinant expression in Rosetta Competent Cells (Millipore, #70954) at 12° C with ampicillin and chloramphenicol selection ...
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bioRxiv - Cell Biology 2019Quote: ... 1000 U/mL mouse recombinant Leukemia inhibitory factor (LIF, ESG1107, Millipore) and Penicillin-Streptomycin-Neomycin Antibiotic Mixture (15640055 ...
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bioRxiv - Biophysics 2021Quote: Recombinant HSA expressed in Pichia pastoris was purchased from Sigma-Aldrich (#A7736 ...
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bioRxiv - Neuroscience 2021Quote: ... and with or without 0.3 µM recombinant BirA (Sigma-Aldrich; SRP0417) (Howarth and Ting ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Systems Biology 2020Quote: ... Recombinants were selected on LB-agar containing 15μg/ml kanamycin (Sigma). Successful integration was confirmed via amplification and sequencing of the flu locus ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant TNFα was purchased from Sigma-Aldrich (St. Louis, MO, USA). The protease inhibitor cocktail was purchased from Thermo Fisher Scientific (MA ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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bioRxiv - Cell Biology 2022Quote: For binding assays with recombinant KAP (Sigma Aldrich, Cat. No. SRP5175) or CAK (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
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bioRxiv - Immunology 2022Quote: ... cells were then stimulated with 100U/ml recombinant IFNα2 (Sigma-Aldrich) to induce transcription from the IFIT1 loci ...
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bioRxiv - Biochemistry 2022Quote: ... 1000 U/mL recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore) and 50 U/mL Penicillin–Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Biochemistry 2020Quote: ... Recombinant rabbit anti-pan-ADP-ribose binding reagent MABE1016 (Merck Millipore) was used 1:1000 ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) for CSR to IgG1 ...
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bioRxiv - Genomics 2022Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Systems Biology 2024Quote: ... and 500 U/mL recombinant leukemia inhibitory factor (LIF; Millipore, ESG1107) at 37 °C in 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) (L-I) ...
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bioRxiv - Biochemistry 2020Quote: ... we intraperitoneally (i.p.) administrated the AMPA/kainate receptor agonist kainate (8 mg/kg; Sigma, St. Louis, MO, USA) dissolved in 0.1 M phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... a selective inhibitor of TGFβ Type 1 receptor kinases (TGFR1i) was purchased from Sigma-Aldrich (St. Louis, MO) and used at a concentration of 10 µM in all experiments.
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bioRxiv - Cancer Biology 2021Quote: ... Zebrafish embryos were treated with the indicated compounds: insulin/IGF1 receptor antagonists BMS-754807 (Sigma-Aldrich #BM0003-5MG) at 7.5μM and NVP-AEW541(Selleckchem # S1034 ...
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bioRxiv - Biophysics 2021Quote: ... The receptor solution was subsequently centrifuged at 130’000 g through a 0.22-μm Ultrafree-MC spin filter (Millipore) for 5 minutes at 4°C in order to remove possible aggregates ...
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bioRxiv - Neuroscience 2022Quote: ... AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptors were blocked using 6,7-dinitroquinoxaline-2,3-dione (DNQX, Sigma) dissolved in dimethyl sulfoxide and diluted by 1000 in aCSF for 10μM bath application.
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bioRxiv - Neuroscience 2023Quote: ... and AMPA/NMDA receptors antagonists (10 µM CNQX and 50 µM DL-AP5, respectively, Sigma-Aldrich, Darmstadt, Germany) were used during the recordings ...
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bioRxiv - Neuroscience 2023Quote: ... Stock solutions of the NMDA receptor antagonist DL-2-amino-5-phosphonovaleric acid (DL-APV; #5282; Millipore Sigma), GABAA receptor antagonist bicuculline methiodide (#14343 ...
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bioRxiv - Biophysics 2023Quote: ... and the Transient Receptor Potential Vanilloid (TRPV4) agonist GSK1016790A (300 nM [Sigma-Aldrich] dissolved in modified Tyrode solution) were applied using a local perfusion system ...
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bioRxiv - Biochemistry 2024Quote: The collected concentrated receptors from the previous step (1.5 mg/mL) were mixed with asolectin lipids (Sigma-Aldrich) solubilized at 5 mg/mL in 5% DDM (Anatrace) ...
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bioRxiv - Cell Biology 2020Quote: ... human Akt1 (Sigma-Aldrich) or 0.3 μg active ...
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bioRxiv - Biochemistry 2022Quote: ... Purified human ApoA1 (Sigma) or purified ApoA1-ApoM protein was resuspended in PBS (10:1 mol/mol ...