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Citations for Millipore Sigma :
1151 - 1200 of 7146 citations for Recombinant Human Interleukin 6 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI, Sigma). Flow cytometry was performed on a MACSQuant Analyzer 10 (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2021Quote: ... 6% (w/v) sodium hydroxide (Sigma) in water ...
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bioRxiv - Microbiology 2021Quote: ... 6-hexanediol reagent (Sigma-Merck, 240117) were heated to 45°C and diluted to 50% in water as stock solution ...
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bioRxiv - Neuroscience 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Sigma).
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bioRxiv - Biochemistry 2019Quote: ... MWCO 6-8 kDa (Millipore Sigma). The 23 kDa A3B-CTD was verified using Coomassie Blue staining after PAGE (data not shown) ...
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bioRxiv - Genomics 2021Quote: ... 6 mM ascorbic acid (Sigma A7631) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and hydrocortisone (Sigma, 10−6 M), supplemented with 20% conditioned media ...
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bioRxiv - Bioengineering 2019Quote: ... 6-diamidino-2-phenylindole (Sigma Aldrich) was utilized to visualize cell nucleus ...
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bioRxiv - Immunology 2020Quote: ... 6’-diamidino-2-phenylindole (DAPI) (Sigma). For immunofluorescence staining primary antibodies were anti-luciferase antibody (ab21176 Abcam ...
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bioRxiv - Microbiology 2020Quote: ... and 6 μM APC (Sigma Aldrich) were added at the time of infection.
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bioRxiv - Microbiology 2020Quote: ... 6 g/liter HEPES (Millipore #391338), 5 g/liter glucose (Sigma-Aldrich #G7021) ...
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bioRxiv - Plant Biology 2021Quote: ... and 6 g/l Gelzan (Sigma) were used for plant growth ...
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bioRxiv - Molecular Biology 2020Quote: ... β 6 genes from Sigma-Aldrich at a concentration of 100μM ...
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bioRxiv - Molecular Biology 2020Quote: ... 6 mM EDTA (E5134, Sigma Aldrich) and 60mM Tris-HCl (M151 ...
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bioRxiv - Microbiology 2021Quote: ... 6-well plates (CLS3516; Millipore Sigma) were seeded with ~3.0 × 105 cells/well and incubated for 48 to 72 h at 37°C in 5% CO2 until 80 to 90% confluency was reached ...
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bioRxiv - Cell Biology 2020Quote: ... and hydrocortisone (Sigma, 10−6 M). Culture media was supplemented with 20% conditioned supernatant from previous EL08.1D2 cultures.
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bioRxiv - Cell Biology 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) (Sigma) was used to eliminate dead cells ...
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bioRxiv - Cell Biology 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) (Sigma) was used to eliminate dead cells ...
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bioRxiv - Neuroscience 2021Quote: ... Two μl of 6-OHDA (Sigma) (5 μg/μl ...
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bioRxiv - Genomics 2022Quote: ... injections of 6-OHDA (Sigma-Aldrich) were performed directly into each lobe of the interscapular BAT ...
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bioRxiv - Cell Biology 2023Quote: ... mouse IL-6 (Sigma-Aldrich, RAB0308), mouse TNF-α (Cayman ...
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bioRxiv - Cell Biology 2023Quote: ... or 6 µL PNGaseF (11365177001, Sigma) and diluted with Lysis buffer to 50 µL ...
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino-2-phenylindole (DAPI, Sigma) and Alexa488 Streptavidin (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 6 (Sigma-Aldrich, C-1909) and incubated at high pressure for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... 6% Ficoll PM-400 (Sigma-Aldrich), 0.2% Sarkosyl (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI, Sigma). Flow cytometry was performed on a MACSQuant Analyzer 10 (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... and 6 μM SB431542 (#S4317, Sigma) were added on day 2 for 24–36 h ...
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bioRxiv - Developmental Biology 2024Quote: ... 6-diamidino-2-phenylindole (DAPI, Sigma). Slides were mounted with Elvanol mounting medium.
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bioRxiv - Developmental Biology 2023Quote: ... Retinol (10-6 M, Sigma-Aldrich) was added in all organotypic cultures from D2 and then every 8 days in order to respect the meiosis entry cycle of spermatogonia (5) ...
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bioRxiv - Physiology 2024Quote: ... 6-9 DBP (Sigma-Aldrich, MO) dosage (100 ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-diamino-2-phenylindole (DAPI) (Sigma) in PBS for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... clone GS-6 (Millipore-Sigma #MAB302) was used at 1 ...
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bioRxiv - Neuroscience 2023Quote: ... clone GS-6 (Millipore-Sigma #MAB302) was used at 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... 6-diamidino-2-phenylindole (DAPI, Sigma) or fixable viability dye 405 (eBiosciences) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 6 mL methanol (99.8%, Sigma-Aldrich), and 8 mL UPW by gravity ...
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bioRxiv - Immunology 2024Quote: ... 6 mM MgCl2 (Sigma-Aldrich, #M1028), 1 M betaine (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant insulin human (≥ 98%), somatostatin-14 (≥ 97%, human HPLC grade), and urocortin-3 (≥ 97%, human HPLC grade) were all purchased from Sigma-Aldrich. Taxonomy will only be indicated for insulin ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with anti-6x His antibody horseradish peroxidase (HRP) conjugate (Sigma A7058-1VL ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant containing 20mM imidazole was incubated with Ni-NTA His•Bind® Resin (Novagen) beads with end-to-end shaking for 4 hr at 4°C ...
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bioRxiv - Immunology 2021Quote: ... the His-tag was cleaved using a Thrombin kit (Millipore Sigma, St. Louis, MO). Confirmation of the His-tag removal was confirmed by W ...
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bioRxiv - Microbiology 2021Quote: ... Cleared lysates were loaded on chromatographic columns filed with His-select resin (Millipore Sigma) or glutathione resin (GE healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Ni-NTA pull down was conducted by using HIS-Select Nickel Affinity Gels (Sigma). After lysing cell pellet with buffer A (6 M guanidine-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... Western blotting was performed using anti His-tag (Cat no. H1029-5ML, Sigma, USA) as well as with anti GST antibody (Cat no ...
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bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich) and 1% antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...