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Citations for Millipore Sigma :
801 - 850 of 7574 citations for Recombinant Human Interleukin 6 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and incubated with recombinant protein (GenScript, Sigma, Tocris) of each analyte for 4 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... recombinant proteins of Ubiquitin (10µg) (Sigma-Aldrich, U6253) or human His6-ubiquitin (Boston Biochem ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SLAMF7 protein (r-SLAMF7) was from Sigma. Purified anti-SLAMF7 (162.1 ...
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bioRxiv - Immunology 2020Quote: ... 5 μg/ml recombinant GAL4-TA (Sigma-Aldrich) was added for an additional 14 hours ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant mouse EGF (0.025μg/ml, Sigma-Aldrich, #SRP3196) and bovine pituitary extract (30μg/ml ...
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bioRxiv - Pathology 2022Quote: ... recombinant anti-Cre (Merck Millipore, 69050, 1:1000) and revealed using Histofine reagent (Nichirei Biosciences) ...
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bioRxiv - Biochemistry 2024Quote: Recombinant plasmids were transformed into Tuner cells (Novagen) in Luria-Bertani (LB ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant IGF-I protein at 10nM (Sigma, #I3769), recombinant IGF-II protein at 10nM (R&D ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-ura / SC-ura + 50µg/ml 6-azauracil (6-AU, Sigma), SC-leu / SC-leu + 0.4mM CuSO4 (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... 6-hydoxydopamine (6-OHDA; cat. #H4381; Sigma-Aldrich, St. Louis, MO) was dissolved in vehicle solution (0.1 % ascorbic acid [cat ...
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bioRxiv - Physiology 2021Quote: ... and IL-6 (KIT ELISA IL-6-RAB0308-1KT, Sigma-Aldrich) in BALF following the kit instructions ...
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bioRxiv - Microbiology 2022Quote: ... The forward primer contained a 6-FAM (6-carboxyfluorescein) tag (Sigma). EMSA reaction mixtures (25 μL volume ...
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bioRxiv - Cell Biology 2024Quote: ... cells were selected with 6 µg/mL 6-thioguanine (Sigma-Aldrich). The generated mutant cells were stocked in CELLBANKER 1 (Nippon Zenyaku Kogyo).
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bioRxiv - Neuroscience 2024Quote: For the intranasal administration of 6-hydroxydopamine (6-OHDA; Sigma-Aldrich), MMTV-PyMT +/-female mice at 1 week after the tumor onset were briefly anesthetized with 3% isoflurane ...
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bioRxiv - Biophysics 2023Quote: ... Both resiniferatoxin (Alomone Labs) and 6’-iodoresiniferatoxin (6’-iRTX, Sigma-Aldrich) were dissolved in ethanol to create a 1 mM stock and subsequently diluted to a 200 nM working concentration with the bath solution ...
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bioRxiv - Microbiology 2022Quote: ... and cultured in RPMI supplemented with 10% FBS and 20 U/ml of Interleukin-2 (IL-2; Catalog no. H7041; Sigma, St. Louis, MO). All the cells were incubated at 37°C in the presence of 5% CO2.
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2021Quote: The inhibitory effect of recombinant P235 on recombinant glycosyl hydrolase was assayed using the β-Glucosidase Activity Assay Kit (MAK129, Sigma). Enzymatic reactions were carried out in K-Phosphate buffer (100 mM ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... Anti-His-specific monoclonal antibodies were from EMD Millipore (prod # 70796). Anti-T7 tag-specific monoclonal antibodies were from Novagen.
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bioRxiv - Neuroscience 2020Quote: ... the long-lasting dopamine receptor ligand [3H] 2-amino-6,7-dihydroxy 1,2,3,4-tetrahydronapthalene (ADTN) (Sigma) was injected to a final estimated concentration of 200 nM ...
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bioRxiv - Immunology 2022Quote: ... The immune sera from each group were treated with receptor destroying enzymes (RDE, Sigma-Aldrich) for 18 h at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... The reconstituted receptors were cleared by moderate centrifugation via spin filter microtubes (Merck Millipore Ltd.) followed by size exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE ...
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bioRxiv - Neuroscience 2022Quote: ... the long-lasting dopamine receptor ligand [3H] 2-amino-6,7-dihydroxy 1,2,3,4-tetrahydronapthalene (ADTN) (Sigma) was injected to a final estimated concentration of 200 nM ...
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bioRxiv - Bioengineering 2021Quote: ... and 50 ng mL−1 receptor activator of nuclear factor kappa-B ligand (Sigma Aldrich). The scaffold was then perfused at 56 μL per second for 28 days in the same medium ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-α1 GABAA receptor subunit (1:300; 06-868, Sigma-Aldrich, St. Louis, USA), guinea pig anti-α2 GABAA receptor subunit (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Glucocorticoid Receptor antibody (#3660, Cell Signalling Technology) or control IgG antibody (#03-198, Merck Millipore) and incubated for 1 h at room temperature whilst rotating ...