Labshake search
Citations for Millipore Sigma :
9951 - 10000 of 10000+ citations for Recombinant Human ROR1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... + 10 mM dithiothreitol to reduce the proteins and then for 30 min at room temperature with 50 mM NH4HCO3 + 50 mM Iodoacetamide (SIGMA) in the dark to alkylate them ...
-
bioRxiv - Cancer Biology 2021Quote: ... Methanol/chloroform extraction was performed on 100ug of protein for each lysate and pellet finally re-dissolved in 100ul of 6M Urea (Sigma), 2M thiourea (Sigma) ...
-
bioRxiv - Cancer Biology 2021Quote: ... was used to determine the concentration of harvested cell lysates and 500 μg of protein lysate was loaded into the supplied spin column (Catch and Release Immunoprecipitation Kit; Millipore). Immunoprecipitation using antibodies against FLAG (Cell Signaling Technology ...
-
bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was resuspended in digest buffer (0.1 M triethylammonium bicarbonate, pH 8.5, Sigma-Aldrich, tandem mass tag (TMT) labeling using a 6-plex TMT kit (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... Digested DNA was then incubated with 50 µl of protein A/G agarose beads and 5 µl anti-S9.6 (Millipore, MABE1095) overnight at 4 °C ...
-
bioRxiv - Biophysics 2022Quote: ... Some samples that were used to obtain or confirm resonance assignments were assembled by mixing protein with equal volume of 1.5 M ammonium sulfate (Sigma-Aldrich), 0.1 M Tris ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 10 μg of proteins were separated by electrophoresis in an 10% SDS polyacrylamide gel and transferred onto a polyvinylidene fluoride membrane (PVDF, Millipore). The membranes were blocked with 5% (v/v ...
-
bioRxiv - Biophysics 2022Quote: ... The purified protein was concentrated up to 5 mg/mL using Centricon YM-10 and Microcon YM-10 concentrators (Amicon, Millipore). Crystals used in this study were obtained under oxygen exclusion in hanging drops from 0.5 M Hepes pH 7.2 ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein was then further purified by concentrating and running over a gel filtration column (CYZ superdex 200 increase, Sigma) in 20 mM HEPES pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The beads were washed three times with 0.5 ml of FLAG wash buffer and the bound protein was eluted with 1X SDS-PAGE loading buffer supplemented with 100 µg·ml-1 3X FLAG peptide (Sigma-Aldrich) on ice ...
-
bioRxiv - Biochemistry 2022Quote: ... PKG Iβ (71-686) protein was concentrated to 20 mg ml-1 by using 30 kDa cutoff Amicon Ultra (Millipore) and initial crystal screening was performed by Mosquito Crystal robot (TTP Labtech ...
-
bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated using a prewashed Amicon Ultra-10 concentrator (molecular weight cut off 30 kDa; EMD Millipore), flash frozen in liquid N2 and stored in -80°C.
-
bioRxiv - Biochemistry 2022Quote: ... The purified protein was concentrated using a prewashed Amicon Ultra-10 concentrator (molecular weight cut off 10 kDa; EMD Millipore), flash frozen in liquid N2 and stored in -80°C.
-
bioRxiv - Biochemistry 2022Quote: Purified full-length E-cadherin was concentrated to 8-10 μM and mixed with the nanodisc scaffolding protein MSP1D1 (Sigma) at a 5 fold molar excess ...
-
bioRxiv - Neuroscience 2022Quote: Tissues from Tmem150c-/- mutants and wild type mice were lysed with 8 M urea buffer and protein concentrations were determined using the Bradford reagent (Sigma). Proteins were separated by SDS-PAGE ...
-
bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
-
bioRxiv - Neuroscience 2022Quote: ... Each 15 μg sample of protein was separated using 8% SDS polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to a nitrocellulose membrane (Sigma). The membrane was blocked with 5% not-fat milk in 0.1M Tris-buffered saline (pH 7.4)/Tween 0,1% (TBSTween ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were resolved by SDS PAGE and transferred to an Immobilon P polyvinylidene fluoride or Immobilon-FL membrane (EMD Millipore)64 ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples were centrifuged 15 min at 13200 rpm at 4°C and protein concentration measured by Bradford assay (Sigma-Aldrich).
-
bioRxiv - Microbiology 2022Quote: ... The cells were sonicated on a Sonics Vibracell VCX750 Ultrasonic Cell Disrupter (Labotal) and BugBuster protein extraction reagent (1:100 ratio) (Novagen) were added to complete the lysis ...
-
bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was collected from the column and concentrated approximately 10-fold using an Amicon Ultra Centrifugal Filter Unit (Millipore) with a 3,000 Da cutoff ...
-
bioRxiv - Biophysics 2022Quote: ... the proteins were expressed in Escherichia coli strain BL21 Star (DE3) cells using 0.4 mM Isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma) for 9 to12 hours at 20 °C ...
-
bioRxiv - Biophysics 2022Quote: ... Protein was concentrated to 30-40 mg ml-1 using a 50-kDa cutoff Amicon Ultra centrifugal filter unit (Millipore). NpSxph crystals were obtained by hanging drop vapor diffusion at 4°C using 1:1 v/v ratio of protein and precipitant ...
-
bioRxiv - Biochemistry 2022Quote: ... Expression of the SSZ-FTpA protein was verified by Western blotting of whole-cell lysate using PAP (Sigma-Aldrich, P1291) antibodies according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2022Quote: ... β-actin protein level was evaluated by western blot analysis of rehybridized membranes with anti-β-actin antibody (Sigma, A5441) at a 1:5000 dilution ...
-
bioRxiv - Biochemistry 2022Quote: ... The column was washed with 30 column volumes (CV) of wash buffer before bound proteins were eluted with wash buffer supplemented with 3 mM D-desthiobiotin (Sigma). The protein was transferred into wash buffer without D-desthiobiotin and incubated overnight at 4°C with 1:100 (w/w ...
-
bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
-
bioRxiv - Cell Biology 2022Quote: ... The purified protein was then concentrated to 300 μM using an Amicon-2 Ultra concentrator (Millipore Sigma, product number UFC2030). DTT was added to the concentrated protein to a final concentration of 1 mM ...
-
bioRxiv - Cancer Biology 2022Quote: ... was added to the culture media for 8 hours before preparing protein lysates with the above lysis buffer supplemented with N-Ethylmaleimide (25mM, Sigma). Lysates were pre-cleared using Protein G sepharose beads (GE Healthcare ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed by separation on SDS-PAGE gels (8-12%) and transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore). Immunoblots were imaged using an anti-Ssa1/2 polyclonal antibody at a 1:10,000 dilution ...
-
bioRxiv - Cell Biology 2022Quote: ... glutathione or flag peptide were removed by buffer exchange and the purified proteins were concentrated on Amicon Ultra 50K filters (Millipore). Purified proteins were recovered in 20 mM Hepes ...
-
bioRxiv - Cell Biology 2022Quote: The induction of the unfolded protein response was carried out by the addition of 5 µg/ml tunicamycin (Sigma-Aldrich) in medium and incubation for 6 h prior cell lysis or the supplementation of pre-warmed medium with 10 mM DTT (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 50μg of protein was resolved on 10–12% SDS-PAGE followed by transferring to nitrocellulose or PVDF membranes (EMD-Millipore). For immunoprecipitation ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins (30 µg) from brain cell lysate were resolved by SDS–PAGE and transferred to Immobilon-P membranes (Millipore Corp.). Membranes were incubated with anti-TSP1 (1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... 50 −100 μg of protein were loaded on a 7%-12% polyacrylamide gel and then transferred on a methanol activated PVDF membrane (Millipore). Blot membranes were blocked for one hr with 5% milk or 5% BSA in TBS with 0.1% Tween 20 (TBST) ...
-
bioRxiv - Developmental Biology 2021Quote: ... As control for iodide uptake and protein-binding measurements cells were also treated with 30µM sodium perchlorate (Nis inhibitor; NaClO4, Sigma-Aldrich)) and 2 µM methimazole (TPO inhibitor ...
-
bioRxiv - Biochemistry 2021Quote: ... and untreated Ycf1 protein (12 μg) sample were incubated for 30 min on ice with trypsin from bovine pancrease (Sigma) at varying concentrations (0 ...
-
bioRxiv - Biochemistry 2021Quote: ... the as-isolated proteins (25 μM) were incubated under aerobic conditions with the ferric chelator desferrioxamine (5–1000 mM, Sigma)(11) ...
-
bioRxiv - Molecular Biology 2021Quote: ... CRY1 proteins were purified using Myc resin (EZview™ Red Anti-c-Myc Affinity Gel, SIGMA-ALDRICH Catalog No: E6654) according to the manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2020Quote: Protein half-life was measured by treating cells with DMSO as control or 100 μg/ml of cycloheximide (Sigma, #C4859) for 30 or 60 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cell debris was removed by centrifugation at 500 g for 10 minutes and filtration through nylon low protein-binding filters (SLHP033RS, Millipore). Virus was concentrated by ultracentrifugation (employing a SW32 rotor at 25K for 2hr and 30min at 4° C ...
-
bioRxiv - Genetics 2021Quote: ... the resin was washed 5 times with ice cold DOC buffer and the bound protein eluted by 15 minute 70°C incubation in 5% w/v sodium dodecyl sulphate (SDS, Sigma). The eluted protein was reduced with 10 mM TCEP and alkylated using 20 mM Iodoacetamide ...
-
bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) (Transgen, CD601-02) and protein expression was induced by an auto-induction system (71757-5, EMD Millipore). Upon overnight induction at 25°C ...
-
bioRxiv - Microbiology 2021Quote: ... After blocking samples were incubated for 120 hours with primary antibody or trimeric RBD protein in PBSGT with 0.1% saponin (S2149, Sigma-Aldrich) on a shaking incubator at 200 rpm ...
-
bioRxiv - Neuroscience 2020Quote: ... The proteins were separated by SDS-PAGE under reducing conditions and transferred to polyvinyl difluoride (PVDF) membranes (Millipore, Billerica, MA). Antibody incubations were performed in 3% BSA in TBS buffer using the following antibodies ...
-
bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
-
bioRxiv - Molecular Biology 2020Quote: ... then 1-2 mg (as indicated) of total protein was incubated with pre-washed M2 α-FLAG magnetic beads (Sigma) or MagStrep “type3” XT beads (IBA ...
-
bioRxiv - Cell Biology 2021Quote: ... protein was dialyzed into NMR buffer [30 mM phosphate buffer pH 7.4 (22.6 mM Na2HPO4 (Sigma-Aldrich, Cat. No. S9763) and 7.4 mM NaH2PO4 (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2021Quote: The coding sequence of Hd3a and RFT1 were cloned into the pCold-GST vector42 and produced as a glutathione S-transferase (GST) fusion protein in Escherichia coli BL21 Rosetta (DE3) (Novagen). Cells were grown in LB medium or minimal medium containing 0.5 g l™1 of 15N-ammonium chloride ...