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Citations for Millipore Sigma :
9851 - 9891 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Tamoxifen induction was performed in mice at 3-5 months of age by oral delivery of tamoxifen (Sigma; 100 mg/kg/day in corn oil) for 4 consecutive days as described previously69 ...
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bioRxiv - Neuroscience 2023Quote: ... minced using sterile razor blades and digested in a 5 mL tube containing 3-5 mL of the digestion mix at 37 °C for 90 minutes: Collagenase type V (Sigma-Aldrich C9263; 5 mg/mL) and Dispase II (Gibco 17105041 ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies employed in this experiment were as follows: mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G8795, 1:4000), anti-Flag (F3165, 1:2000) from Sigma-Aldrich (St. Louis, MO, USA); mouse anti-hemagglutinin (HA ...
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bioRxiv - Molecular Biology 2023Quote: ... Tomato plants were placed into 121 L transparent methacrylate chambers containing hydrophilic cotton swabs soaked with 5 μM of (Z)-3-hexenyl butyrate (HB; Sigma-Aldrich, Saint Louis, MO, USA) or distilled water in the case of control plants ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were trimmed down to a 3-5 mm region around the area of interest and embedded in 5% (w/v) agarose (A9539; Sigma-Aldrich, St. Louis, MO, USA) in deionized water ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% [v/v] Triton X–100) and incubated at 37°C for 3 h either with α–mouse plus and α–rabbit minus for PLA assay (Sigma–Aldrich, 681 Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution as described (Pasternak et al ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Ethanol 95% (v/v) and (-)-nicotine hydrogen tartrate salt ((-)-1 -Methyl-2-(3-pyridyl) pyrrolidine (+)-bitartrate salt were purchesed by Sigma Aldrich (St. Louis, MO, USA). The CB1/2 receptor agonist (R)-(+)-[2,3-Dihydro-5-methyl-3-(4-morpholinylmethyl)pyrrolo[1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenylmethanone mesylate ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human mesenchymal stem cells (MSCs) were cultured on gelatin coated flasks for 2-3 days in Alpha-MEM (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded in microslide channels at a final concentration of ∼5×105 cells/ml in 120 µL cell culture media containing 40 μM BrdU:BrdC mix (3:1, Cat.# B5002, Sigma and Cat.# 284555, Santa Cruz Biotec) and grown for 17-24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were anesthetized and the regenerating part was dissected and collected into 0.5 ml of 2/3 PBS (Sigma D8537, diluted by RNase-free water) with Actinomycin D (ActD ...
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bioRxiv - Microbiology 2023Quote: ... Single colony was picked up into 50 ml 3-(N-morpholino) propane sulfonic acid medium (MOPS) buffered defined liquid medium (Sigma-Aldrich, St. Louis, MI, USA). For DSM1092 ...
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bioRxiv - Cell Biology 2024Quote: ... HBSS in the apical and basolateral chambers was replaced with HBSS containing FITC-dextran 3–5 kDa (FITC-Dex) (Sigma-Aldrich, St. Louis, MO, USA), fluorescein sodium (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... To avoid unspecific labelling the sections were first incubated for 2 × 10 minutes in a solution of PB with 3% hydrogen peroxide (Merck Life Science AS/Sigma Aldrich Norway AS, Oslo, Norway), and next incubated for 1 hour in a solution of PB with 5% normal goat serum (Abcam Cat# ab7481 ...
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bioRxiv - Molecular Biology 2023Quote: ... preadipocytes were induced to differentiate using induction medium (DIFF1) containing growth medium supplemented with 0.5 mM 3-Isobutyl-1-Methylxathine (IBMX) (Sigma-Aldrich, St. Louis, MO, USA I5879), 1 μM Dexamethasone (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... the brain sections were rinsed in phosphate buffer (PB) and incubated 1 h in a solution containing equal volumes of 3% potassium ferrocyanide (Sigma-Aldrich, Ontario, Canada, cat# P9387) and 4% osmium tetroxide (EMS ...
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bioRxiv - Biochemistry 2024Quote: ... six whole blood units were processed as above and suspended in a custom AS-3 formulation containing 50%L1,2-13C2-Glucose (Sigma Aldrich, St. Louis, MO, United States). When calculating % 13C enrichment as a fraction of the total isotopologues ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were dissected into fragments approximately 9 mm3 in size and placed in glyoxal acidic solution (3% w/v glyoxal [Millipore Sigma 128465], 20% ethanol [Fisher BP2818100], 0.75% acetic acid [Millipore Sigma A6283], 5mM NaOH [Millipore Sigma 72068], in ddH2O) for 15 min at 4C ...
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bioRxiv - Microbiology 2022Quote: ... The paraformaldehyde was then removed and the cells were washed twice with 100 μl of 1XPBS and stained with100 μl of DAPI (Sigma Aldrich catalog no. 28718-90-3) working solution (600 nM DAPI ...
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bioRxiv - Developmental Biology 2020Quote: ... in TNT for 2-3 h at room temperature and incubated with an anti-Fluo-POD antibody (1/50, Sigma-Aldrich REF 11 426 346 910) in 2% TNB overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: The method of multiple inductions of acute endotoxaemia comprises intraperitoneal administration of 8 consecutive injections (every 3 days) of LPS 10 mg/kg (Lipopolysaccharides from Escherichia coli 026:B6, Sigma-Aldrich®, St. Louis, MO, USA), in saline (NaCl 0,9%) ...
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bioRxiv - Immunology 2019Quote: ... Then the sections were washed 3 times in 1×PBS (10 min each wash) and the nuclei were counter-stained with DAPI (D9542-50, Sigma-Aldrich Corp., St. Louis, MO, USA) for 10 minutes of incubation in a dark chamber ...
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bioRxiv - Immunology 2020Quote: ... A 3-fold serial dilution of the sera was performed and HRP goat anti-mouse γ-chain was used (SIGMA, Saint Louis, MO, United States A9044). The result was registered using a microtitration plate reader (Anthos 2010® ...
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bioRxiv - Immunology 2020Quote: ... After dialysis external solutions were mixed and were concentrated by Centricon YM-3 (from Merck-Millipore, used at 5000 x g for 30 minutes, 4 °C) to reach 1 ml as final volume ...
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bioRxiv - Bioengineering 2022Quote: ... Tissue sections were then washed in 1x PBS (3×5 min) before incubation with donkey anti-chicken IgY 647 (Sigma-Aldrich, St. Louis, MO, United States) and donkey anti-mouse IgG 488 (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Birds from the anxiogenic group received 2.5 mg/kg of β-CCM (β-carboline-3-carboxylic acid-N-methylamide [FG 7142], Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) dissolved in DSMO (Dimethyl Sulfoxide suitable for HPLC ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Lipids were extracted from the cell pellets through one-phase extraction with 202 uL of BuOH:MeOH (1:1) (Sigma 71-36-3 and 67-56-1) that contains 10 umol of cholesterol-d7 (Cayman Chemical 83199-47-7 ...
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bioRxiv - Microbiology 2023Quote: ... slick SSW and non-slick SSW from sites #1 – #3 (Fig. 1b) were fixed with 25 % glutardialdehyde (0.5 % final concentration, Sigma-Aldrich/Merck Life Science AB, Solna, Sweden) and stored at −80 °C ...
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bioRxiv - Physiology 2024Quote: ... The injected hormone solutions were prepared from crystallised powder (T3: CAS number 6893-02-3, Sigma-Aldrich, St. Louis, MO, USA and T4: CAS number 51-48-9, Sigma-Aldrich, St. Louis, MO, USA). The amount of hormone injected (T4 ...
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bioRxiv - Neuroscience 2024Quote: ... mitoEGFP mice 3 to 5 months of age (mos) received single intraperitoneal injections of 1mg/kg of Lipopolysaccharide (LPS) (Millipore Sigma, strain O55:B5, L2880-25MG) or saline ...
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bioRxiv - Plant Biology 2024Quote: ... and 400 seeds from each pool were then grown for 4 days in the dark in liquid media after sterilization and stained with Ruthenium Red dye (Verger et al., 2016) Liquid media was removed and 3 ml of Ruthenium Red dye (Sigma Corporation, 0.5 mg/ml in dH2O) was applied to seedlings for 2 min in a 10 ml microtiter growth plate ...
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bioRxiv - Plant Biology 2020Quote: The AspN-digested acyl-ACP products were separated on a reversed-phase column (Discovery® BIO Wide Pore C18; Sigma-Aldrich, 10 cm, 2.1 mm, 3 mm) using acetonitrile:10 mM ammonium formate (90:10 v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... heterozygote (HNF4α fl/+, Cre/+), and knockout (HNF4α fl/fl, Cre/+) mice (N=3-6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 to a final concentration of 0.2nM and PCR amplified for cloning into T7 phage vector arms (Novagen/EMD Millipore Inc. T7 Select 10-3 Cloning kit). Detailed protocol can be found in dx.doi.org/10.17504/protocols.io.j8nlkkrr5l5r/v1 ...
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bioRxiv - Cell Biology 2023Quote: ... ∼1 kb 5’ homology arm and ∼1 kb 3’ homology arm of msps genomic region were amplified from the genomic DNA (Sigma, Extract-N-Amp™ Tissue PCR Kit), mutated to be insensitive to gRNAs ...
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bioRxiv - Neuroscience 2023Quote: ... Nunc glass bottom dishes with an inner diameter of 27 mm were coated with Poly-L-Ornithine (Sigma-Aldrich P4957, 0.01% stock diluted 1:3 in PBS) overnight at 37° C ...
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bioRxiv - Cell Biology 2020Quote: ... blocking was performed in 3% BSA in PEM80 for 45 minutes and incubated overnight with a primary antibody against αTub (mouse IgG1, Sigma Aldrich, B-5-1-2, dilution 1:500). The cells were again washed with PBS (3×5 min ...
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bioRxiv - Neuroscience 2021Quote: Whole brains from E13 Asxl3+/+ and Asxl3fs/fs mice were homogenized in RIPA buffer supplemented with protease inhibitor cocktail and phosphatase inhibitor cocktail 3 obtained from Sigma-Aldrich (P8340 and P0044; St Louis, MO, USA). Protein levels were normalized after BCA analysis (Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... 40 uL of the supernatant was transferred to a 96 well plate to which 20 uL of 200mM 3-nitrophenylhydrazine hydrochloride (Sigma Aldrich; dissolved in 50% ACN and 50% water) and 20 uL of 120 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (Pierce ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were fixed with 3.7% paraformaldehyde and washed 3 times with PBS before and after incubation with mouse anti-FLAG M2 primary antibody (Sigma, diluted 1:2000 in PBS with 1% BSA) or HRP-conjugated anti-mouse IgG secondary antibody (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... and Mannitol (3 g of Bacto Peptone [Becton Dickinson], 5 g of Yeast Extract [Sigma-Aldrich], 25 g of D-Mannitol [Sigma-Aldrich], 1 L of Milli-Q water). Liver Infusion Broth (Becton Dickinson) ...
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bioRxiv - Microbiology 2024Quote: ... or Envelope T9I expression constructs (in total 3 µg DNA/well) in 6-well format by using per well 10 µL 1x polyethyleneimine-hydrochlorid (PEI) (Sigma-Aldrich, #764965-1G, 1 mg/mL in H2O) and 500 µL Opti-MEM reduced serum media (Gibco ...