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Citations for Millipore Sigma :
9751 - 9800 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... 3051.2) for 5 min and blocked in PBS containing 3% bovine serum albumin and 1% normal goat serum (Sigma-Aldrich, Taufkirchen, Germany, A4503 & G0023) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... larval zebrafish were mounted in a custom 3D printed chamber (SLS Nylon 12, 3DPRINTUK, London, United Kingdom) in 3% low-melting point agarose (Sigma-Aldrich, St. Louis, Missouri) at 5 dpf and allowed to recover overnight before functional imaging at 6 dpf ...
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bioRxiv - Microbiology 2023Quote: ... Cells from these cultures were used for growth experiments in Tibbles Rawling (TR) salts minimal medium57,58 containing either 0.1% (weight/volume) algae-derived alginate (referred to as “alginate”) (Sigma Aldrich, CAS-number 9005-38-3) or 0.1% (weight/volume ...
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bioRxiv - Physiology 2024Quote: ... for 10 days and treated with 100 nM endothelin-1 (ET1) or vehicle (H2O) the last 3 days as previously described.25 Cardiomyocytes were then stained for α-actinin (Sigma, SAB2108642, 1:800) and N-cadherin (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... for 1-3 hours at room temperature (RT) and then permeabilized with 0.1% (v/v) Triton X-100 (Sigma-Aldrich, Cat # 9036-19-5) for 1 hour before blocking with 3% (w/v ...
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bioRxiv - Bioengineering 2024Quote: ... Phosphatase activity was determined by a chromogenic reaction using 5-bromo-4 chloro-3 indolyl phosphate and nitroblue tetrazolium (Sigma, St. Louis, MO, USA) as substrates.
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bioRxiv - Molecular Biology 2024Quote: ... treated with lysis buffer (RIPA buffer, 1x Roche Complete Protease Inhibitor cocktail, 1X Sigma Phosphatase Inhibitor Cocktail 2, and 1X Sigma Phosphatase Inhibitor Cocktail 3), and incubated on ice for 30 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... cochleae were washed 3 times for 15 minutes in PBS and the sensory epithelium was dissected off and mounted (Sigma-Aldrich, Cat. No. F6182) on a glass slide ...
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bioRxiv - Cell Biology 2024Quote: Alveolar organoids were removed from Matrigel by mechanical dissociation with a cut pipette tip or retrieved from HA hydrogels with 2% hyaluronidase (Sigma Catalog #37326-33-3) for 30 minutes at 37°C followed by mechanical dissociation with a pipette tip ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were washed with DPBS five times for 3 min each, and stained with 4’, 6-diamidino-2-phenylindole (DAPI, 1 μg/mL in PBS) (Sigma, San Francisco, CA, USA) in the dark at room temperature for 8 min ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were washed 3x with PBS prior to an incubation in permeabilization solution (0.1% Triton X-100 [Sigma-Aldrich, #T8787] and 3% BSA [Sigma-Aldrich, #10735078001] in PBS) for 5 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... on day 3 of activation (Fig. S1A) 250 µl of concentrated virus was preincubated with 8 µg/mL polybrene (EMD Millipore, TR-1003-G) for 30 min on ice ...
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bioRxiv - Bioengineering 2023Quote: ... Confluent monolayers were treated with 8-(4-chlorophenylthio)adenosine 3′,5′-cyclic monophosphate sodium salt (cPT-cAMP; Sigma-Aldrich, cat# C3912, 25-250 µM) Ro 20-1724 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution ...
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bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by the addition of 28 μl of a stop reaction mixture: 3 μl of the reducing reagent (mixture of 0.2 g 1-amino-2-naphtol-4-sulfonic acid (Millipore-Sigma, Burlington, MA, USA, #08751) with 0.2 g sodium bisulfite (Millipore-Sigma ...
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bioRxiv - Biophysics 2023Quote: ... was purchased from MedChem-Express (Monmouth Junction, NJ, USA). The phospholipid 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC, see Fig. 1) was purchased from Sigma-Aldrich (Merck KGaA, Germany). Potassium bromide (KBr) ...
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bioRxiv - Microbiology 2023Quote: ... The CpG with a sequence of 5’-TCC.ATG.ACG.TTC.CTG.ACG.TT-3’ (all nucleotides are phosphorothioate-modified at the 3’ inter-nucleotide linkage) was purchased from Integrated DNA Technologies (IDT, Coralville, IA) and the IFA from Sigma-Aldrich (St. Louis, MO). We used the CpG-IFA as adjuvants for the intramuscular injection because they have been shown to induce Th1 dominant immune responses (68 ...
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bioRxiv - Plant Biology 2023Quote: ... in a petri dish and then placed in 12-well plates containing 90% liquid MS medium (1/4 strength) and 10% polyethylene glycol (PEG, MW 8000, SIGMA, cat # 25322-68-3) (p/v ...
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bioRxiv - Cancer Biology 2023Quote: ... des-Arg9-Leu8]-BK (B1R antagonist) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzyl indazole were purchased from Sigma-Aldrich (St. Louis, MO, USA). 8-Bromoguanosine 3′ ...
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bioRxiv - Immunology 2023Quote: ... 300,000 WT T84 cells per well in a 6-well plate were treated with 20 µL lentivirus using 3 µL Polybrene transfection reagent (Sigma Aldrich #TR-1003-G) diluted in 3 mL media ...
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bioRxiv - Microbiology 2022Quote: ... parahaemolyticus strains were grown in Luria Bertani (LB) medium (Fischer Scientific, Pittsburgh, PA) containing 3% NaCl (LBS) at 37°C with aeration or M9 medium media (Sigma Aldrich, St. Louis, MO) containing 3% NaCl (M9S ...
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bioRxiv - Microbiology 2023Quote: Transductions were carried out at 3×105 cells/mL and the indicated MOIs under normal culture conditions supplemented with 5 µg/mL polybrene (Sigma-Aldrich, TR-1003-G). Culture medium was replaced ∼24 hours after transduction and antibiotic resistance selection was carried out using 1.2 µg/mL puromycin for 2 days ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... an isolated infrarenal aortic segment of C57BL/6J mice was perfused with sterile isotonic saline containing type I PPE (2-3 U/ml, E1250, Sigma-Aldrich, Burlington, MA, USA) for 5 min under pressure (120 mmHg ...
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bioRxiv - Microbiology 2023Quote: ... Boost vaccination was performed with the homologous virus inactivated at 4°C for 3 days with 0.1% beta propiolactone (BPL) (Sigma-Aldrich Corporation, St. Louis, MO) as previously described (66) ...
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bioRxiv - Neuroscience 2023Quote: ... induction to an EB-like state was initiated by changing the medium to chemical induction medium (StemFit AK02N, (Ajinomoto) with 3 μM SB431542 (Sigma-Aldrich, St. Lous, MO), 3 μM CHIR9901 (Cayman ...
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bioRxiv - Cell Biology 2023Quote: ... the cell lines were treated in triplicate with a top dose of 10uM in 1:3 dilutions for a total of 12 doses with paclitaxel (Sigma-Aldrich Product Number T7191), fulvestrant (Sigma-Aldrich Product Number I4409) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... isolated areas on the slides were then placed in PBS for 3 minutes before permeabilizing with 0.5% Triton-X 100 (Sigma-Aldrich, St. Louis, MO, USA) for 20 mins ...
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bioRxiv - Neuroscience 2023Quote: ... and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine, Sigma-Aldrich, Cat# F0503 and Uridine, Sigma-Aldrich, Cat# U3003-5G). 120 µL of cell suspension was carefully plated onto each coverslip and allowed to adhere for 2 hours at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... 215 uL of either ice-cold 20mM Tris-HCl/2 mM MgCl2/0.5% Triton X-100 with protease inhibitor (Pierce #A32953) and phosphatase inhibitor cocktail 3 (Sigma #P0044; Buffer #1, Bf#1) or boiling 1%SDS/50mM NaF (Buffer #2 ...
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bioRxiv - Physiology 2023Quote: ... In order to substitute cholesterol in the patch with cholest-4-en-3-one (cholestenone) the bath solution was replaced with 2 mM MBCD solution saturated with cholest-4-en-3-one (Millipore-Sigma, St. Louis, MO).
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bioRxiv - Microbiology 2023Quote: ... freshly drawn whole blood was mixed with an equal volume of a solution containing 3% dextran (Sigma-Aldrich, ACS Cas.# 9004-54-2, Denmark) and 1.8% sodium chloride (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Microbiology 2023Quote: ... We serially diluted the extracts and plated 20 µL on 10 % TSB agar (3 g/L tryptic soy broth and 15 g/L agar, both Sigma-Aldrich, St. Louis, USA) plates (12 x 12 cm ...
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bioRxiv - Bioengineering 2023Quote: ... 850365C) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2K; 880129C) (Sigma Aldrich, St Louis, MO, USA) lipids were combined in a molar ratio of 90:10 and prepared using the thin film hydration method ...
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bioRxiv - Evolutionary Biology 2023Quote: ... an animal was chemically cross-linked for one hour at room temperature in 30 mL of PBS 1X added with 3% formaldehyde (Sigma – F8775 - 4×25 mL). Formaldehyde was quenched for 20 min at RT by adding 10 ml of 2.5 M glycine ...
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bioRxiv - Bioengineering 2024Quote: ... dissociated hiPSC-Cardiomyocytes and primary cardiac fibroblasts were mixed in a 3:1 ratio and resuspended in fibrinogen by mixing the cell solution with 33 mg/mL human fibrinogen (Sigma-Aldrich, cat. no. F3879) and RPMI-B27 (described above ...
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bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Cell Biology 2023Quote: ... . Ultrathin sections placed on grids were stained with 1% aqueous uranyl acetate (Agar Scientific, Stansted, UK) and 3% lead citrate (Sigma Aldrich, St. Louis, USA), and observed at the FEI Morgagni 268D microscope.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 3 (10 mg/kg) intraperitoneally (i.p.) 30 min prior to injection of zymosan (Sigma-Aldrich; 2 mg/ml in saline, 0.5 ml, i.p.). Mice were sacrificed (by CO2 inhalation ...
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bioRxiv - Developmental Biology 2024Quote: ... For live confocal imaging of dat:eGFP and dat:mito-RFP dopaminergic neurons larvae were mounted in 1.2% low-melting point agarose in E3 embryo media containing 160ug/ml Ethyl 3-aminobenzoate methanesulfonate (Tricaine MS-222; Sigma-Aldrich 886-86-2) Tricaine anesthetic ...
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bioRxiv - Cell Biology 2024Quote: ... Phase separation of lipids from the serum was performed using an organic phase of diisopropyl ether: n-butanol (3:2; Sigma Aldrich, 296856, B7906, respectively). Organic and aqueous phases were allowed to mix at room temperature with continuous stirring in the dark for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-5000] (DPPE-PEG5K) and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline was procured from Fisher Scientific (Pittsburg ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 1.5-week-old Arabidopsis seedlings pooling 3 seedlings/extraction for each transgenic lines and using Spectrum™ Plant Total RNA Kit (Sigma-Al-drich, STRN250), RNAs were on-column treated with the DNase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... cells then fixed for 5 min with 4% PFA followed by 3 times of PBS wash and then permeabilized with 0.1% of Triton X-100 (Sigma-Aldrich, Cat. no. X100-00ML) in PBS for 5 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... The sample was centrifuged at 500 x g for 5 minutes at 4°C after which the supernatant was discarded and the nuclei resuspended in 3 ml ice cold nuclei suspension buffer (1x phosphate-buffered saline [PBS, Sigma-Aldrich, P4417-50TAB, US]), 0.01% bovine serum albumin [BSA ...
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bioRxiv - Cell Biology 2024Quote: ... the cell culture medium was removed and replenished with a culture medium respectively containing carbonyl cyanide 3-chlorophenylhydrazone (CCCP; Sigma-Aldrich, MA, USA, #C2759), and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid acetoxymethyl ester (BAPTA-AM ...
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bioRxiv - Molecular Biology 2024Quote: ... then fully resuspended in 1 mL cold resuspension buffer supplemented with 1% each phosphatase inhibitor cocktails 2 and 3 (Sigma Cat #P5726 and P0044). To lyse the resuspended cells ...
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bioRxiv - Bioengineering 2024Quote: ... a 5 µL drop of the formulation was placed on a glass slide that was silanized by liquid phase deposition in a solution of 2% 3-(trimethoxysilyl)propyl methacrylate (cat. #M6514, Sigma-Aldrich, Oakville, Ontario, Canada) prepared in toluene (cat ...
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bioRxiv - Cell Biology 2024Quote: ... Auxin-inducible degradation of proteins was induced by treatment with 0.5 mM indole-3-acetic acid (IAA) (Sigma, I2886-5G, CAS: 87-51-4) and 4 µM phytic acid dipotassium salt (Sigma ...