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Citations for Millipore Sigma :
9451 - 9500 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips was functionalized by amine using the silanization solution of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (Sigma-Aldrich, 8191720100), acetic acid ...
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bioRxiv - Cell Biology 2023Quote: ... For NMR-experiments the proteins were concentrated by repeated ultrafiltration (Amicon Ultra-4 Ultracel-3 kDa centrifugal filter device, Merck Millipore, Burlington, USA).
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bioRxiv - Cell Biology 2023Quote: ... for 3 h in serum- free RPMI-1640 (with 10,000 U/mL Penicillin, 10 mg/mL Streptomycin, 1:100) (Sigma-Aldrich, Sydney, Australia) at a density of 0.2 × 106 cells/cm2 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with PBS and DNA content were visualized by DAPI (1 μM; Cat# D9542, Millipore Sigma, I h) staining at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... then permeabilized in 200 μl of PBS1X supplemented with 3% fetal bovine serum and 0.1% saponin (Sigma-aldrich, Saint-Louis, MO, USA) for 5 min at room temperature (20–25 °C) ...
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bioRxiv - Genomics 2022Quote: The stable cell lines constantly expressing the minigenes were subjected to four time points (0, 3, 6, and 9 h) of post actinomycin D treatment (final concentration of 1 μg/mL; Sigma, no. A9415) treatment in three biological replicates ...
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bioRxiv - Genomics 2023Quote: ... Sections were rinsed in Phosphate Buffered Saline (PBS) and blocked in a humidity chamber in 3% normal donkey serum (Sigma-Aldrich, 566460) and 0.3% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... animals were amputated to induce Erk activation and then dissociated at 3 hpa in CMF supplemented with a 1:10 dilution of Liberase TH (Millipore Sigma, 5401135001) into single cells (Witchley et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Blots were washed again 3 times for 10 min each and developed using Immobilon Luminata Crescendo Western HRP substrate (Millipore, Cat#WBLUR0500). Western blots were imaged and quantified using ImageLab (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... were transferred to 35 mm diameter petri dishes containing 3 ml of liquid GM supplemented with 10 μM estradiol (β-Estradiol, E8875, Sigma Aldrich, freshly prepared by diluting a 1 mM stock solution in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... with secondary antibody in PBST containing goat serum, washed (3 × 5 min), stained (10 min, 20°C) with DAPI (1 µg/ml in PBS; Sigma, Cat# D9542) and washed (5 min ...
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bioRxiv - Bioengineering 2023Quote: ... tissues were washed with the isopropanol (IPA) for 3 hours then rehydrated in DI and treated with 50U/ml DNase (Millipore Sigma, #10104159001) for 8 hours followed by an overnight DI rinse ...
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bioRxiv - Physiology 2023Quote: ... Mice were anesthetized with 3% isoflurane and an osmotic minipump (Alzet model 2004, #0000298) set to deliver (±)-isoproterenol hydrochloride (Sigma-Aldrich #I5627) dissolved in sterile saline at a dose of 70mg/kg/day for 2 weeks was inserted subcutaneously via a small midline incision in the back ...
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bioRxiv - Bioengineering 2023Quote: ... sections were stained with Oil Red O (3 mg/mL Oil Red O in 60% isopropanol; Sigma-Aldrich, St. Louis, MO, USA) for 6 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for ms-gamma-tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig-CENP-C 1:250 (MBL ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours post-knockdown and induction coverslips were fixed in -20oC methanol for 3 minutes and stained for ms-gamma tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig CENP-C 1:25 (MBL ...
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bioRxiv - Cell Biology 2023Quote: ... Cover glasses were then activated by silanization with an ethanol solution supplemented with 1% v/v (3-Aminopropyl) trimethoxysilane (Sigma Aldrich, 281778) for 15 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... the medium was changed to 2i + LIF medium consisting of base medium supplemented with CHIR99021 (Chiron, 3 µM, Sigma-Aldrich SML1046-5MG), PD0325901 (1 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with primary antibody (15 µg/mL in 3% BSA in PBS): rabbit anti-KCNN4 antibody (IgG, clone HPA053841, Sigma Aldrich®) or control rabbit antibody (IgG ...
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bioRxiv - Bioengineering 2023Quote: ... and stained for 15 minutes in Alcian Blue solution (1% Alcian Blue in 3% solution of acetic acid) (Cat. # A5268, Sigma Aldrich, UK). Slides were then washed in running tap water for 2 minutes ...
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bioRxiv - Physiology 2023Quote: ... MEFs were selected with 3 μg/mL of puromycin and transduction efficiency for mCherry was assessed by flow cytometry using Guava EasyCyte™ (Millipore, USA). A transduction efficiency of 95% or greater was achieved.
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... SDS samples containing 4∼8 μg total proteins were separated with 12% HEPES-Tris PAGE gel (MEILUNBIO, MA0243) and transferred to polyvinylidene difluoride membranes (Millipore, 70584-3). The membrane was blocked with 5% skim milk in TBST buffer (20mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μL of freshly dissolved histones was added to a premixed solution containing 3 μL of 10 mM Cy5-azide (Sigma-Aldrich, 777323), 10 μL of a 3:7 mixture of 50 mM CuSO4 and 100 mM THPTA ...
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bioRxiv - Cell Biology 2023Quote: ... Digested tubules were strained on a 100 μm strainer containing 3 ml of FBS (100% Fetal Bovine Serum, heat inactivated, Sigma F4135-500mL). Total germ cells were centrifuged at 300 xg for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended at 10 million cells per ml and DAPI added immediately before FACS (Sigma-Aldrich; final concentration of 3 μM). Live ...
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bioRxiv - Cell Biology 2023Quote: ... H3.3 K36M or cells overexpressing histones were continuously grown in the presence of 50μM Indole-3-acetic acid (IAA) (Sigma-Aldrich, I5148-2G) until experiments were performed to suppress protein expression ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated in 1xPBS + 0.5% Tritton X-100 + 3% Bovine Serum Albumin before adding the primary antibody (HPA036814, Sigma-Aldrich – 1/1000) for 48h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... cumulous-enclosed egg complexes were collected from the oviduct and cultured in 37°C MEM with hyaluronidase (3 mg/ml, Sigma Aldrich, H4272) for 2 min ...
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted to a starting OD600 of 0.01 in 3 mL of BHIS supplemented with increasing concentrations of 1) bile salt mixture (50% cholate and 50% deoxycholate; Sigma-Aldrich B8756), 2 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were again washed with TBS-T for 3 times and were incubated with Immobilon Forte Western HRP substrate (WBLUF0500, EMD Millipore Corporation). Images were captured using AI600 Imager from GE Healthcare Life Science.
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bioRxiv - Plant Biology 2023Quote: ... Separate 50 µl (same aliquot from the total 300 µl) were separated on 13% SDS-PAGE gel for immunoblot using anti-Histone 3 (Sigma #06-755) at 1:3000 dilution with 1:10000 anti- rabbit secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were then rinsed 3 times with PBS and mounted onto microscope slides with 5 μl Mowiol 4-88 (Sigma-Aldrich #81381). Cells were visualized and imaged with a Nikon Ti-Eclipse A1M Confocal Microscope using a 60X objective ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... viability measurements were performed for the 1 vpc and 3 vpc conditions of all vectors and two wells of uninfected cells with the Cell Counting Kit 8 (CCK8, Sigma-Aldrich #96992). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were blocked in 3% BSA for 30 min at RT and incubated with anti-alpha-SMA antibody (1:1000; Sigma, Cat. # A2547) and anti-collagen type I antibody (1:1000 ...
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bioRxiv - Systems Biology 2023Quote: ... the 2x 50 μl were pooled and then split in 3 x 33 μl which were each dialyzed against 50 mL of MilliQ H2O (Merck Millipore, Darmstadt, Germany) as described above ...
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bioRxiv - Immunology 2024Quote: ... AAS99264.1 and AAO88201.1 respectively) and divided into 3 pools (15-mers overlapping by 10 aa, Sigma-Aldrich, United States or Mimotopes, Australia). For each serotype ...
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bioRxiv - Microbiology 2024Quote: ... lysing the host cells via syringing with 20 and 25-gauge needles and filtering through a 3 μm Isopore™ membrane filter (Millipore; # TSTP02500). 3.5×105 parasites from each sample were then added into a T175 flask containing confluent HFFs in D10 medium ...
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bioRxiv - Plant Biology 2024Quote: AM 810–813 plasmids (Table S2) encoding active and proteolytically dead (PD) GST-tagged AtATG4A and AtATG4B were used to transform Rosetta (DE3) cells (Novagen, 70954-3). Expression was induced overnight at 16°C using 0.25 mM IPTG (Hellobio ...
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bioRxiv - Cell Biology 2024Quote: ... Gels were transferred back to a 24- well plate and incubated with 0.5 ml PBS BSA 3% with secondary antibodies AlexaFluor™ 488 goat anti-rabbit (A11008, Sigma, 1:500), Cy3-streptavidin and 0.1 μg/ml DAPI (D9542 ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used for immunofluorescence were: anti- cleaved Caspase-3 (Asp175) (1:400, rabbit, CST, #9661) and anti-FLAG (1:400, mouse, Sigma-Aldrich, #F3165) for detection of overexpressing proteins ...
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bioRxiv - Neuroscience 2024Quote: ... The coverslips were then washed for 3 x 5-minutes washes in Duolink Wash Buffer A and then incubated in 40 μL of polymerase (Sigma Aldrich, DUO82028) diluted 1:80 in 1X amplification buffer (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... NPC-line 3: NPCs derived using the protocol of Shi et al., (Shi et al., 2012) from hiPSC line IPSC0028 (Sigma-Aldrich, RRID:CVCL_EE38). Line 1 NPCs were generated as previously described from hiPSCs grown on a mouse embryonic fibroblast (MEF ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were washed for 3 × 5 minutes in Duolink Wash Buffer A and then incubated in 40 μL of ligase (Sigma Aldrich, DUO82027) diluted in 1X ligation buffer (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... To prevent movements and EMG contamination of the phrenic neurogram pancuronium bromide was administered (3 mg/kg IV, Sigma-Aldrich, St Louis) to achieve neuromuscular blockade ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Biophysics 2024Quote: ... All solutions were prepared with ultrapure water (18.2 MΩ cm of resistivity) from a Milli-Q Integral 3 purification system (Millipore, Bedford, MA, USA).
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bioRxiv - Immunology 2024Quote: ... IL-6 and IL-17A were assessed using the High Sensitivity T-cell Discovery Array 3-Plex (Millipore, St. Charles, MO, USA) at Eve Technologies using the Bio-Plex™ 200 system (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Peritoneal macrophages (PerMacs) were collected from the peritoneal cavity via gavage 4 days after intraperitoneal injection of 3% brewer thioglycolate medium (Sigma-Aldrich, B2551) and plated in in medium with 10% FBS ...