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Citations for Millipore Sigma :
9701 - 9750 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS (3 times x 5 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... All the ASO probes were synthesized with biotin-TEG added to the 3’ end of the sequences (Sigma-Aldrich). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... We used 3 and 5wt% GelMA with a 1:16 mass ratio of photoiniator (Lithium phenyl-2,4,6-trimethylbenzoylphosphinate, LAP; Sigma Aldrich). LAP and GelMA were dissolved together in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Cancer Biology 2024Quote: ... mitotically active cells were identified using a rabbit anti-phosphohistone-3 antibody (Ser10, Millipore 06–570, 1:500 dilution), with a Cy3-donkey anti-rabbit immunoglobulin G secondary antibody (the Jackson laboratories ...
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Evolution of polyamine resistance in Staphylococcus aureus through modulation of potassium transportbioRxiv - Microbiology 2024Quote: ... Four of the cultures were diluted 1/100 into fresh TSB containing 2mM of spermine (Sigma, 71-44-3), and two of the cultures were diluted 1/100 into plain TSB as a control ...
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bioRxiv - Microbiology 2024Quote: ... The cell nucleus was stained with DAPI (1:1000 dilution, Sigma-Aldrich 28718-90-3, St. Louis, MO, USA) at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the culture medium was changed to medium supplemented with 500 μM 3-Indoleacetic acid (IAA; Sigma-Aldrich, I2886-5G) and 1 μg/mL doxycycline (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 x in TBS-Tween (0.1%) + 2% BSA before incubation in TRITC conjugated anti-Mouse secondary antibody (Sigma) at 1:1000 concentration for 1 hr in the dark ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... and then blocked for 3 hr at room temperature or overnight at 4 °C in BSA (Sigma-Aldrich A2153) 3% in PBS-T ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 99.7% pure), Bisphenol TMC (Catalog #, 52746-49-3) and 2′,7′-Dichlorodihydrofluorescein diacetate (Catalog #, D6883) were purchased from Millipore Sig ma (MO ...
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bioRxiv - Cell Biology 2024Quote: ... The chips were then washed with PBS 3 times before incubation with 0.5% (v/v) glutaraldehyde (Sigma-Aldrich, G6257) in PBS at RT for 10-15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... they were first anesthetized using egg water containing 0.016% tricaine (3-amino benzoic acid ethyl ester) from Sigma-Aldrich. Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... 50-60 µm cryosections were blocked for 3h at room temperature in PBS containing 3% BSA (A7906, Sigma-Aldrich) and 0.5%Triton X-100 (T9284 ...
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bioRxiv - Cell Biology 2024Quote: ... Dried lipids were resuspended in 15µL chloroform and 65µL of click mix containing 400µM 3-Azido-7-hydroxycoumarin (Baseclick #BCFA-047-1) and 900µM tetrakis(acetonitrile)copper(I) tetrafluoroborate (Sigma #677892) in acetonitrile/ethanol (7:3 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibodies (anti-TDP-43 1:750, PTGLabs; anti-SUMO2/3 [8A2] 1:500 Sigma) diluted in blocking buffer (1.5% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... and the chocolate-flavored pellets were replaced with cotton pads infused with 1 ml of a 1% solution of 2,3,5-trimethyl-3-thiazoline (TMT, Sigma Aldrich) in water ...
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bioRxiv - Microbiology 2024Quote: ... Cells were maintained in the TREx BCBL-1-RTA medium with additional 3 μg/mL puromycin (P7255, Sigma-Aldrich) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: Eggs were collected from female Xenopus laevis frogs 16-18 hours after egg laying was induced by injection of 500 U of human chorionic gonadotropin (Chorulon from Merck, Sigma #CG10-10VL). Cytostatic factor-(CSF- ...
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bioRxiv - Cell Biology 2020Quote: ... Serum-free DMEM was then discarded and cells were incubated in serum-free DMEM containing 120 μg/mL human HDL (Sigma-Aldrich, L1567) for 6 h ...
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bioRxiv - Immunology 2021Quote: ... fresh living donor biopsies or cells remaining following 10X cell capture for sequencing were resuspended in 90% human serum (Sigma, cat# H4522) and 10% DMSO for cryopreservation and cooled to -80°C in a Mr.Frosty (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for immunostaining: anti-CREST/anti-centromere antibodies (human, Antibodies Inc.; 1:100) anti-Tubulin (mouse monoclonal, Sigma; 1:5000). DNA was stained with 0.5 µg/ml DAPI (Serva ...
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bioRxiv - Bioengineering 2019Quote: ... human skeletal muscle samples were minced and then dissociated into a single cell slurry with Clostridium histolyticum collagenase (Sigma, 630 U/mL) and dispase (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were blocked for 30 minutes with mouse serum and immunostained with directly Cy3-conjugated anti-human HNA (Millipore, MAB1281C3, 1:100). Nuclei were counterstained using DAPI ...
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bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
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bioRxiv - Bioengineering 2019Quote: ... were maintained as monolayer cultures in flasks pre-coated with 60 μg/mL solution of human placental collagen type IV (Sigma Aldrich, MO) and grown in bronchial epithelial growth medium (BEGM ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we grew PAO1-pvdSi in iron-limited CAA medium (using 100 mg ml−1 human-apo transferrin to bind iron and 20mM NaHCO3 as cofactor, both from Sigma-Aldrich, Switzerland), supplemented with various amounts of purified pyoverdine (pyoverdine concentration in μM ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for the analysis of human synovium with microscopy: rabbit polyclonal anti-CD3 (Sigma-Aldrich Cat# C7930, RRID:AB_259074), anti-CD103 (Clone EP206 ...
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bioRxiv - Bioengineering 2021Quote: ... for 12 days in SSC expansion media with the following additions: 100 ng/mL follicle stimulating hormone from human pituitary (FSH, Millipore Sigma, F4021), 10 ng/mL luteinizing hormone from human pituitary (LH ...
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Pathology 2021Quote: HIF-1α protein expression was depleted in human U937 cell line (ATCC, USA) using shRNA targeting HIF-1α (TCRN0000010819, Sigma Aldrich, USA) using a liposome mediated delivery (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... which was un-exposed European pooled serum.23 Secondary detection was with Human IgG (H&L) Antibody Peroxidase Conjugated (Sigma – Aldrich, Germany), developed with Pierce™ TMB Substrate Kit (Thermo Fischer ...
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bioRxiv - Neuroscience 2020Quote: ... and pre-absorption of mouse anti cathepsin-S with 300 nanograms of active human cathepsin-S (SRP0292, Sigma-Aldrich, St. Louis, MO) did not result in detectable immunolabeling signal.
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bioRxiv - Microbiology 2020Quote: ... Parasites were washed thrice for 15 min in PBSTx and incubated for 24 hrs in 1:2000 Fab specific FITC conjugated goat anti human IgG (F5512, Sigma-Aldrich UK) diluted in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... Freshly washed erythrocytes were diluted to 1% hematocrit in 1X PBS containing 1% BSA and coincubated for an hour with antibodies against the human glycophorin AB (GYPAB) (1:400, Sigma Aldrich UK), GYPA (Clone E4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for immunostaining include rabbit anti-human/mouse PKD-1 (4502371) and mouse anti-mouse α-smooth muscle actin(A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Cell Biology 2021Quote: ... media was changed to serum-free media (DMEM, 2mM L-glutamine) overnight followed by insulin stimulation (100nM human insulin, Sigma-Aldrich, I9278), with 30-minute vehicle ...
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bioRxiv - Neuroscience 2020Quote: ... and COs derived from H9 hESCs and iPSCs were measured using the Human Oxidative Phosphorylation Magnetic Bead Panel Milliplex MAP Kit (Millipore–H0XPSMAG-16K), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and Human Kidney Cells (HKC) (acquired from Collaborator Dr. Kim Rathmell Lab, Vanderbilt University, TN) were maintained in DMEM (Sigma-Aldrich, # D6429) media supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... human muscarinic 1,2,3,4, and 5 acetylcholine receptors (M1R, M2R, M3R, M4R, and M5R) with acetylcholine (ACh; Sigma-Aldrich A6625, in measuring buffer), murine μ-opioid receptor (MOP ...
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bioRxiv - Cell Biology 2021Quote: ... Insulin secreted from the islet grafts was measured in the mouse serum three weeks post-transplant using a human insulin ELISA (Millipore, EZHI-14K).
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bioRxiv - Immunology 2022Quote: ... The inner surface of Transwell inserts (3.0 μm pore transparent polyester membrane) pre-coated with 100 μl of human collagen IV solution (34 μg/ml; Sigma-Aldrich, USA) were seeded with 100 μl of an enteroid fragment suspension ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Serum was collected from groups of mice and an in–house assay was used in which anti–human IgG Fc (Sigma, Catalog # FAB3700259) was used to capture human IgG (MS-Hu6) ...
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bioRxiv - Immunology 2022Quote: ... 2×105 cells of each of transfected and untransfected cells stained with SARS-CoV-2 Spike antibody (SinoBiological) and Anti-human IgG FITC (Sigma-Aldrich®). After staining with primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were blotted onto nitrocellulose membranes and probed for each antigen using the following antibodies at the indicated dilutions: Human PEAK1 (Millipore 1:400), Mouse PEAK1 (Abgent 1:400) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed and then sequentially incubated with anti-SARS-CoV-2 CR3022 mAb [24] (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma, Cat#A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... were measured with a human cytokine/chemokine panel utilizing Luminex 200 xMap technology and the MILLIPLEX MAP ® multiplex kit (Luminex xMAP technology; EMD Millipore, Chicago ...
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bioRxiv - Bioengineering 2021Quote: ... The binding of VHH-Fcs/ACE2-Fc to S1 was detected using 100 µL 1 µg/mL HRP-conjugated goat anti-human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were washed five times and incubated for 1 h with 1 µg/mL HRP-conjugated goat anti- human IgG (Sigma, Cat#A0170). Finally ...
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bioRxiv - Microbiology 2022Quote: ... 5 mL of whole human blood was layered onto 5 mL of Histopaque-1077 Hybri-MaxTM (Sigma Life Science, H8889-100 mL) in a column ...
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bioRxiv - Evolutionary Biology 2019Quote: HSA-AAG containing medium is RPMI 1640 growth medium with the addition of 341 mM HSA (human serum albumin, Sigma, Cat # A9511) and 1 mg/ml AAG (human a1-acid glycoprotein ...