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Citations for Millipore Sigma :
9551 - 9600 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
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bioRxiv - Neuroscience 2024Quote: ... before being cut into 250μm parasagittal slices using a McIlwain tissue chopper and placed on Millicell membrane (3 to 4 slices each per animal, 0.4 μm membranes, Merck Millipore) in 50% BME (41010026 ...
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bioRxiv - Molecular Biology 2023Quote: ... pregnant females were ovariectomized on E2.5 and afterwards injected with 3 mg medroxyprogesterone 17-acetate subcutaneously (Sigma, M-1629) on E3.5 and E5.5 ...
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bioRxiv - Neuroscience 2024Quote: ... cells grown in 96-well plates were treated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, Hungary) in a final concentration of 200 μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... The female individuals were anesthetized with 0.02% (w/v) ethyl 3-aminobenzoate methanesulfonate (Sigma-Aldrich, St. Louis, MO, USA), euthanized by decapitation ...
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bioRxiv - Molecular Biology 2024Quote: ... were grown for 2 hours on 3 ml of LB with 0.2% arabinose before placing them on a patch consisting of 1% agarose (Sigma) in water ...
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bioRxiv - Biochemistry 2024Quote: ... and 3 µg standards (manually mixed, Avanti Polar Lipids® and Sigma-Aldrich®; BVO from Spectrum Chemical®) onto a Merck HPTLC glass plate silica gel 60 (20×10cm ...
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bioRxiv - Microbiology 2024Quote: ... Oat seedlings for purification and amplification were treated with 3 mM maleic hydrazide (Sigma-Aldrich, St. Louis, MO, USA) at emergence ...
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bioRxiv - Cell Biology 2024Quote: ... permeabilized using 0.2% Triton-X100 in 1x phosphate buffered saline (PBS) for 15 min and blocked using 3% BSA (Sigma) in 1x PBS for 30 min ...
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bioRxiv - Biophysics 2023Quote: Tricaine stock was prepared by dissolving 400 mg of Tricaine powder (ethyl 3-aminobenzoate methanesulfonate; Millipore Sigma, #E10521-50G) with 97.9 ml of deionized water and 2.1 ml of 1 M Tris ...
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bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...
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bioRxiv - Cell Biology 2024Quote: ... were washed 3 x with PBS followed by incubation with 20 μg mouse anti-FLAG M2 Ab (Sigma Aldrich) for 2 h at 4 °C with rotation prior to 3 x PBS washes and resuspension in PBS ...
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bioRxiv - Genetics 2024Quote: ... gcry1:BFP-3’mitfaHom plasmid was then added to the injection mix along with phenol red (Sigma-Aldrich P0290). The final concentrations injected into the embryos were ...
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bioRxiv - Immunology 2024Quote: ... PCR was performed to add the 3XFlag tag to the N terminus of ZBP1 cDNA at the 5’ Not1 site and 3’ Sal1 site to facilitate cloning into expressing vector p3XFlag-CMV-7.1 (Sigma). The resulting plasmid containing the 3XFlag-ZBP1 was further subcloned in the Bst1 and BamHI sites of the vector pLVX-EF1α-AcGFP1-C1 (Takara ...
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bioRxiv - Microbiology 2024Quote: ... The antigen-bound magnetic beads were washed five times with lysis buffer and eluted with 2.5 volumes of 3× FLAG peptide (150 ng/μl, Sigma) for 3 h at 4°C with gently shaking ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2024Quote: ... Flat mounts were incubated in 300 µL of blocking buffer (3% (W/V) bovine serum albumin (BSA) (Catalog #BP9703-100) and 0.1% (V/V) Triton X-100 (Sigma) in HBSS (Fisher Scientific Catalog # MT21023CV ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by staining of ossified bones with a 50 mg/l of alizarin red S (Sigma, #130-22-3) solution in 2% KOH for 3 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... reconstituted in 3 µL of 66% acetonitrile and analyzed by a ZIC-HILIC column (150 × 2.1 mm, EMD Millipore) coupled with a Q Exactive HF Orbitrap MS (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... HUVECs were seeded into the collagen gel channel with media containing 3% dextran (Leuconostoc spp., Mr 450,000–650,000, Sigma-Aldrich) at a density of 1 × 107 cells/mL ...
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bioRxiv - Biochemistry 2024Quote: Exoproteome fractions were concentrated to 1 mg mL-1 total protein (approximately 10 times concentration) using an Amicon Ultra-0.5 Centrifugal Filter Unit with a molecular weight cutoff of 3 kDa (Merck Millipore). A 10 µL aliquot of the concentrated exoproteome fraction was reduced with 5 mM tris (2-carboxyethyl ...
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bioRxiv - Biochemistry 2024Quote: ... Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP, Sigma Aldrich) dissolved in 100 mM Tris ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and 3-(4,5-dimethyl-2-thiazolyl)2,5-diphenyl-2-H-tetrazolium bromide (MTT, Sigma-Aldrich) solution (final concentration ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
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bioRxiv - Biophysics 2024Quote: ... Mice were decapitated and the nose was dissected en bloc and embedded in 3% low-grade agar (A7002, Sigma) prepared with Ringer’s saline solution containing (in mM) ...
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bioRxiv - Molecular Biology 2024Quote: ... The linear DNA backbone was then extracted from the gel via electroelution in D-tubes (EMD Millipore 71508-3), followed by phenol chloroform extraction and butanol concentration.
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bioRxiv - Cancer Biology 2023Quote: ... Tricaine stock was prepared by dissolving 400 mg of Tricaine powder (ethyl 3-aminobenzoate methanesulfonate; Millipore Sigma, #E10521-50G) with 97.9 ml of deionized water and 2.1 ml of 1 M Tris ...
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bioRxiv - Neuroscience 2023Quote: ... was concentrated to under 100 μL in 0.5 mL 3 kDa molecular weight cut-off centrifugal concentrators (Millipore, UFC500396) (Surface sample in Fig 4C) ...
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bioRxiv - Microbiology 2023Quote: ... The harvested cells were resuspended in immobilised metal chromatography (IMAC) buffer (50 mM Tris-HCl [pH 7.5], 300 mM NaCl) supplemented with 3 kU/ml rLysozyme (Novagen). The cell suspension was sonicated on ice (Ultrasonic disruptor UD-201 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were anaesthetised in a solution of buffered ethyl 3-aminobenzoate methanesulfonate salt (100 mg/L; MS-222, Sigma) in seawater ...
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bioRxiv - Molecular Biology 2023Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide (MTT) cell growth kit (CT02, Millipore, MA, USA) was used for measuring cell viability ...
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS and blocked at room temperature for 1 h using either 3% bovine serum albumin (BSA, Sigma) or 5% goat serum according to the primary antibody used ...
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bioRxiv - Cell Biology 2023Quote: ... 1 3 v/v) at 80°C for 2 h after addition of 50 nmol 5α-Cholestane (Sigma, C8003) as internal standard for neutral sterol analysis ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 7.5×107/15ml RPMI+3%FBS and stimulated with 25nM phorbol 12-myristate 13-acetate (PMA; Millipore-Sigma #P8139) Plates were incubated at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... then fixed with 4% paraformaldehyde for 30 min at room temperature and washed 3 times in PBS.Cells were permeabilized with 0.1% Triton-X100 (Sigma T8787) and washed 3 times in PBS ...
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bioRxiv - Immunology 2023Quote: ... which was precoated for 3 hours at 37°C with 4ug/mL fibrinogen (Sigma-Aldrich; Saint Louis, MO, USA). After letting the cells settle for 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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bioRxiv - Neuroscience 2022Quote: ... The dopamine- synthesis inhibitor 3-Iodo-L-tyrosine (3IY; CAS: 70-78-0, Sigma Aldrich; concentration: 5 mg/ml) was added to samples of 2 ml yeast solution ...
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bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report63 ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (ATCC, HTB-55) were maintained in Eagle’s minimum essential medium (EMEM) (Sigma-Aldrich, Cat# M4655-500ML) containing 10% FBS and 1% PS ...
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bioRxiv - Genomics 2022Quote: ... An antibody specific for GroEL (rabbit polyclonal, 1:10,000 in 3% BSA/TBS-T; Sigma-Aldrich, #G6532-5ML, RRID:AB_259939) with an anti-rabbit IgG (goat polyclonal ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were permeabilized with 0.01% Triton X-100 in PBS for 15 minutes and blocked with 3% bovine serum albumin (A7906, Sigma) in 0.01% Triton X-100 in PBS (blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... embryos were mechanically dechorionated and anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine, Sigma-Aldrich). Zebrafish embryos were individually infected by microinjection with 1 nl of P ...
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bioRxiv - Bioengineering 2022Quote: ... 300 μL of supernatant was filtered under 3 kDa through Amicon Ultra-0.5 mL Centrifugal Filters (Millipore, Burlington, MA) or 96-well plates (Pall Corporation ...
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bioRxiv - Microbiology 2022Quote: ... Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to MTT formazan conversion assay (Sigma-Aldrich), as described (60) ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h in 20 mL TBS-T with 3% milk powder and 1:10,000 diluted goat anti-rabbit IgG-HRP (Sigma). Finally ...
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bioRxiv - Immunology 2023Quote: ... RIPA 400 mM NaCl, (3) Sarkosyl Buffer (2 mM EDTA, 50 mM Tris-HCl, 0.2% sarkosyl (Lauroylsarcosine sodium salt (Sigma)) ...
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bioRxiv - Microbiology 2022Quote: ... cultures were pelleted by centrifugation at 1,600 rpm for 3 min and resuspended in a 10× volume of 5% sorbitol (Sigma), followed by incubation at 37°C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... they were contained in the bath solution and then recorded using the following concentration: Gadolinium chloride (GdCl3) (0.1, 0.3, 1, 3, and 10 μM, Sigma, G7532), Na-gluconate (gluconate ...