Labshake search
Citations for Millipore Sigma :
9501 - 9550 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... sections were rinsed with PBS 3 times and then mounted on microscope glass slides with Mowiol (Sigma-Aldrich, 81381). The expression of proteins was visualized with a Leica TCS SP5X microscope and images were processed with ImageJ (version 1.53f51) ...
-
bioRxiv - Developmental Biology 2022Quote: ... with 3 mg/mL of bovine serum albumin (BSA) and 100 µM of IBMX (Sigma-Aldrich, Merck, Darmstadt, Germany), and collected ...
-
bioRxiv - Cell Biology 2022Quote: ... D-PBS was replaced with ice-cold blocking solution (3% BSA [Sigma, A3803] in D-PBS, without Ca2+/Mg2+) using a Dragonfly Discovery liquid dispenser (SPT Labtech ...
-
bioRxiv - Cell Biology 2022Quote: ... The beads were washed with IP lysis buffer and incubated with 5 mg ml−1 3× FLAG peptide (Sigma–Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... the pellet was resuspended in 3 mL Nuclear Permeabilization Buffer (1X PBS, 5% Bovine Serum Albumin, 0.2% IGEPAL CA-630 (Sigma), 1 mM DTT ...
-
bioRxiv - Cell Biology 2022Quote: ... was revealed with 5-bromo-4-chloro-3-indolyl β-D galactoside (X-gal) (Sigma-Aldrich, Carlsbad, CA, USA). Diploid cells were grown on SC medium supplemented with 20 mg/L adenine hemisulfate ...
-
bioRxiv - Cell Biology 2022Quote: ... activated eggs were cultured for 3 additional hours in KSOM + amino acids media (Sigma Aldrich, Cat# MR-106-D). For parthenogenetic activation of eggs ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with 50 µL of a 2 mg/mL solution of (3-(4,5-dimethyl-thiazole-2-yl)-2,5-biphenyl tetrazolium (MTT, Sigma-Aldrich) in PBS for 4 h and then exposed to 100 µL dimethyl sulfoxide (DMSO ...
-
bioRxiv - Plant Biology 2022Quote: ... After addition of 10μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) solution (5mg/ml, Sigma-Aldrich, USA) the cells were incubated in darkness for another 4 hrs ...
-
bioRxiv - Microbiology 2022Quote: Cells were then pelleted at 7000 × g for 3 min and resulting supernatants filtered through a 0.2 μm PVDF filter (Millipore). The no bacteria “fresh” 5-FU controls were also filtered in this way to account for any potential loss of 5-FU by filtration ...
-
bioRxiv - Microbiology 2022Quote: The effect of CAPG depletion on viability of KO and siRNA-treated cells was assessed at several timepoints using a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay (11465007001; Sigma-Aldrich) per manufacturer directions ...
-
bioRxiv - Neuroscience 2022Quote: Recordings were then performed in oxygenated aCSF superfused at a rate of 2-3 ml/min and supplemented with 100 µM picrotoxin (Alomone Labs) and 0.5-1 µM Strychnine (Sigma). The internal solution was the same as above with 2-4 mM QX314 to block Na+ channels from driving AP firing ...
-
bioRxiv - Biochemistry 2022Quote: ... or 300 mM (octamer-mix + APLFAD) ammonium acetate at pH 7.5 using 3 kDa MWCO Amicon Ultra Centrifugal Filter Units (Merck Millipore). After buffer exchange the volume of each sample was ∼40 µL ...
-
bioRxiv - Microbiology 2022Quote: ... 5 mg/ml of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, St. Louis, MI, USA) solution was applied to the cells and incubated for an additional 4h ...
-
bioRxiv - Synthetic Biology 2022Quote: ... The silanization solution was freshly prepared by mixing MilliQ water with 0.4% (v/v) 3-(Trimethoxysilyl)propylmethacrylat (TMSPMA, Sigma) and 0.4% (v/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... mice were intraperitoneally injected with tamoxifen (0.08mg/g body weight per day for 3 days; Sigma-Aldrich, T5648-1G). For chemical-treated animals’ assay ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were harvested and lysed by 3 cycles of thawing and freezing followed by treatment with benzoase nuclease (Millipore). After pelleting down cell debris (16.9 g/rcf ...
-
bioRxiv - Neuroscience 2023Quote: ... and then received 0.1mL of either 1.5-3% Fluorescein isothiocyanate–dextran (FITC) or Texas Red dextran (70kDa, Sigma-Aldrich #46945 and Thermofisher D1830 ...
-
bioRxiv - Neuroscience 2022Quote: ... Fish were then anesthetized by applying 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521) to the fish mounted in agarose ...
-
bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 0.5 mL centrifugal filter units (3-kDa molecular weight cutoff, Millipore), using centrifugation at 14,000 g ...
-
bioRxiv - Neuroscience 2023Quote: ... From day 3 to day 15 1µM retinoic acid (SigmaAldrich, R2625) and 1µM Sonic Hedgehog Signaling Agonist (Millipore, 566660) were added ...
-
bioRxiv - Plant Biology 2023Quote: ... above the tips with the help of forceps and placed into a 35-mm-diameter dish containing a 70 µm strainer and 3 ml enzyme solution (1.25% [w/v] Cellulase [“ONOZUKA” R10, Yakult], 0.1% [w/v] Pectolyase [P-3026, Sigma-Aldrich] ...
-
bioRxiv - Systems Biology 2023Quote: Buffy Coat was diluted with PBS at a 1:3 dilution and underlayed with Histopaque®-1077 (Sigma-Aldrich), centrifuged at 350g for 30 minutes at 24°C with no brake ...
-
bioRxiv - Microbiology 2022Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was precoated with fibronectin (3 μg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report70 ...
-
bioRxiv - Neuroscience 2023Quote: ... 100 μg/mL streptomycin and 1 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic sodium monophosphate salt (dbcAMP) (Sigma®). Cells were maintained with the differentiation medium at 37 °C in 5% CO2 for 3 days.
-
bioRxiv - Microbiology 2024Quote: ... The antigen-bound magnetic beads were washed five times with lysis buffer and eluted with 2.5 volumes of 3× FLAG peptide (150 ng/μl, Sigma) for 3 h at 4°C with gently shaking ...
-
bioRxiv - Physiology 2022Quote: ... cells were treated with the medium supplemented with 0.5 mM 3’-Isobutyl-1-methylxanthine (IBMX) (Sigma, Cat. I7018-1G), 1 µM dexamethasone (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... Passive dextran permeability was measured by culturing iBECs on Ø 0.4 μm pore polyester or polycarbonate Transwell inserts and cells were exposed to 0.5 mg/mL fluorescein isothiocyanate (FITC)-conjugated 3 – 5 kDa dextran (Sigma) for 24h ...
-
bioRxiv - Cell Biology 2022Quote: ... 6- to 8-week-old mice of the indicated genotypes were intraperitoneally injected with 2.5 ml 3% thioglycollate (T9032, Sigma). On day 4 after the thioglycollate injection ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with DPBS for 5 min and blocked in 1% BSA (Sigma-Aldrich, cat# A4503) in PBS-T (DPBS with 0.05% Tween 20 ...
-
bioRxiv - Microbiology 2022Quote: ... 2 mL aliquots were taken from the main culture for chemical fixation with 3% paraformaldehyde (PFA, Sigma-Aldrich, #F8775) at 20 min at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
-
bioRxiv - Neuroscience 2022Quote: ... then selected after 24-48 hrs for 3 to 14 days with 8 µg/mL of puromycin (Sigma-Aldrich).
-
bioRxiv - Plant Biology 2022Quote: Analytical reference standards were used for the following analytes: indole-3-acetic acid (IAA; Sigma-Aldrich St. Louis, MO), N-(3-indolylacetyl)-DL-aspartic acid (IAA-Asp ...
-
bioRxiv - Developmental Biology 2022Quote: ... BP310-1], 10 mM NaCl [Fisher, Cat. M-11624], 3 mM MgCl2 [Sigma, Cat. M8226], 0.1% IGEPAL [v/v; Sigma, I8896] ...
-
bioRxiv - Developmental Biology 2022Quote: ... 10 mM HEPES-KOH, pH 7.2 [Fisher, Cat. BP310-1], 10 mM NaCl [Fisher, Cat. M-11624], 3 mM MgCl2 [Sigma, Cat ...
-
bioRxiv - Bioengineering 2022Quote: ... MSCs were frozen at passage 3 with 1.25*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% dimethylsulfoxide (DMSO ...
-
bioRxiv - Molecular Biology 2024Quote: ... were grown for 2 hours on 3 ml of LB with 0.2% arabinose before placing them on a patch consisting of 1% agarose (Sigma) in water ...
-
bioRxiv - Biochemistry 2024Quote: Exoproteome fractions were concentrated to 1 mg mL-1 total protein (approximately 10 times concentration) using an Amicon Ultra-0.5 Centrifugal Filter Unit with a molecular weight cutoff of 3 kDa (Merck Millipore). A 10 µL aliquot of the concentrated exoproteome fraction was reduced with 5 mM tris (2-carboxyethyl ...
-
bioRxiv - Biochemistry 2024Quote: ... Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP, Sigma Aldrich) dissolved in 100 mM Tris ...
-
bioRxiv - Molecular Biology 2024Quote: ... The linear DNA backbone was then extracted from the gel via electroelution in D-tubes (EMD Millipore 71508-3), followed by phenol chloroform extraction and butanol concentration.
-
bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS and 3-(4,5-dimethyl-2-thiazolyl)2,5-diphenyl-2-H-tetrazolium bromide (MTT, Sigma-Aldrich) solution (final concentration ...
-
bioRxiv - Cell Biology 2024Quote: ... After blocking of endogenous peroxidases (3% H2O2) and unspecific antibody binding (2% BSA+5%serum) PLAC8 antibody (HPA040465, Sigma) or SARS-CoV-2 Spike Glycoprotein S1 antibody (GTX632604 ...
-
bioRxiv - Neuroscience 2024Quote: ... The device was then pulsed with 3 bolus each of 4 different concentrations of dopamine hydrochloride (Sigma, H8502-10G), 0.5 μM ...
-
bioRxiv - Developmental Biology 2023Quote: ... followed by staining of ossified bones with a 50 mg/l of alizarin red S (Sigma, #130-22-3) solution in 2% KOH for 3 hours ...
-
bioRxiv - Cancer Biology 2023Quote: ... reconstituted in 3 µL of 66% acetonitrile and analyzed by a ZIC-HILIC column (150 × 2.1 mm, EMD Millipore) coupled with a Q Exactive HF Orbitrap MS (Thermo Fisher ...
-
bioRxiv - Biophysics 2024Quote: ... Mice were decapitated and the nose was dissected en bloc and embedded in 3% low-grade agar (A7002, Sigma) prepared with Ringer’s saline solution containing (in mM) ...
-
bioRxiv - Cancer Biology 2024Quote: ... HUVECs were seeded into the collagen gel channel with media containing 3% dextran (Leuconostoc spp., Mr 450,000–650,000, Sigma-Aldrich) at a density of 1 × 107 cells/mL ...
-
bioRxiv - Cell Biology 2024Quote: ... Flat mounts were incubated in 300 µL of blocking buffer (3% (W/V) bovine serum albumin (BSA) (Catalog #BP9703-100) and 0.1% (V/V) Triton X-100 (Sigma) in HBSS (Fisher Scientific Catalog # MT21023CV ...
-
bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with blocking buffer (PBS containing 0.1% Triton X-100 and 3% fatty-acid-and globulin-free BSA [A7030, Lot No. SLCH7959, SIGMA]) for 1 h at room temperature (approximately 26-28°C ...