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Citations for Millipore Sigma :
9501 - 9550 of 10000+ citations for Recombinant Mouse Efna1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... or AAVretro-EF1a-Cre primary antiserum (1:100 mouse-anti-Cre Recombinase antibody, clone 2D8, Millipore Sigma Cat# MAB3120) mixed with donkey serum ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The following primary antibodies were used for immunoblotting at the indicated dilutions: mouse monoclonal anti-FLAG (Sigma-Aldrich, F1804), 1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained for intracellular markers with anti-α-SMA (mouse IgG2a, Sigma Aldrich, St. Louis, MO, 1/250), anti-desmin (rabbit polyclonal IgG ...
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bioRxiv - Microbiology 2022Quote: ... Immunized mice sera followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Antibody complexes were detected using the ImmobilonTM Western systems (Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibody staining for Dlg1 (1:50; mouse, Developmental Studies Hybridoma Bank) and GFP (1:500; rabbit, EMD Millipore) was done in PBT (PBS/0.1% BSA/0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed 3 times in PBS and incubated at room temperature for 30 min with a goat anti-mouse Ig polyclonal secondary antibody conjugated with alkaline phosphatase (0.5 µg/mL, Sigma). After 3 washes with PBS ...
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bioRxiv - Immunology 2020Quote: ... for 1 hour at RT and detection of anti-mouse IgG by the addition of pNPP substrate (Sigma-Aldrich). An arbitrary number of ELISA units were assigned to the reference pool and OD values of each dilution were fitted to a 4-parameter logistic curve using SOFTmax PRO software ...
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bioRxiv - Developmental Biology 2021Quote: ... 293T or AL1 cells were incubated for 3 hours with anti-Myc mouse monoclonal antibody (Sigma-Aldrich; 1:500), and secondary staining was performed using anti-mouse AlexaFluor568 antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the incubation with the primary antibodies: mouse anti-GAD 67 primary antibody (Millipore, Mab5406, 1:1000 dilution), and rabbit anti-GFP primary antibody (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: E14 mouse embryonic stem cells (ESCs) were grown in two different media: ‘serum+LIF’ consisted in Glasgow medium (Sigma), 15% FBS (Gibco) ...
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bioRxiv - Cell Biology 2019Quote: Mouse anti-α-tubulin (DM1A; 1:1000 for indirect immunofluorescence staining [IF]; Cat#T9026, Sigma-Aldrich, St. Louis, MO), rat monoclonal anti-α-tubulin (YL1/2 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was blocked with 3% milk in PBS + 0.05% Tween-80 (PBST) and then incubated overnight with primary antibodies (monoclonal mouse anti-FLAG [to detect MurJ; Sigma] ...
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bioRxiv - Neuroscience 2021Quote: ... was injected at P7 and/or P8 for the GdnfCreER line at 0.1 mg/g mouse using 10 mg/mL Tamoxifen dissolved in sunflower oil (Sigma) with 10% ethanol ...
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bioRxiv - Immunology 2021Quote: ... The bound antibodies were revealed using a HRP-conjugated goat antibody to mouse Ig absorbed with human Ig (Sigma) (1:4,000 dilution) ...
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bioRxiv - Microbiology 2021Quote: ... After incubation at room temperature for 2 hr with a 1:5,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Immunology 2020Quote: ... The plates were incubated sequentially with 500 ng/mL previously described mouse anti-ZIKV mAb ZV-165 and horseradish-peroxidase (HRP)-conjugated goat anti-mouse IgG in PBS supplemented with 0.1% (w/v) saponin (Sigma) and 0.1% bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... spirochetes were incubated for 60 min with goat anti-mouse gold-labeled antibody at 1:30 dilution (G7652; Sigma). The grid was further washed ...
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bioRxiv - Cell Biology 2020Quote: ... Subsequently the PLA protocol was followed according to the Duolink In Situ Red Starter Kit Mouse/Rabbit (Millipore Sigma) instructions ...
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bioRxiv - Cell Biology 2021Quote: ... rat anti-mouse RIPK3 (WEHI clone 8G7; produced in-house7 and soon available from Millipore as MABC1595; 1:2000); rabbit anti-phospho-T231/S232 mouse RIPK3 (Genentech ...
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bioRxiv - Immunology 2021Quote: ... Anti-rabbit IgG-HRP (Cat no.- A0545) and Anti-mouse IgG-HRP (Cat no.- A9044) were purchased from Sigma. Anti-mouse IL-10 ELISA set (Cat no ...
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bioRxiv - Cell Biology 2021Quote: ... the following primary antibodies were used at the indicated dilutions: mouse anti-γ-tubulin 1:500 (T5326; Sigma Aldrich), rabbit anti-mCherry 1:500 (ab167453 ...
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bioRxiv - Genetics 2019Quote: ... The following primary antibodies were used: mouse anti-acetylated tubulin (clone 6-11-B1, Sigma-Aldrich, T6793; 1:1,000), mouse-anti beta-actin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... while mouse primary astrocytes (~3.5 × 105 cells) were seeded on poly-L-lysine coated transwell filters (3450; Sigma-Aldrich) and maintained in astrocyte-media ...
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bioRxiv - Cell Biology 2021Quote: ... the oocytes were washed three times and incubated with FITC-labelled mouse anti-α-tubulin (1:200, Sigma-Aldrich) for 1 h at 37°C.
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bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
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bioRxiv - Genetics 2020Quote: ... blots were incubated with the following antibodies: monoclonal mouse anti-GFP (Sigma, MO; clone 7.1 and 13.1, 1:1000), mouse anti-Tubulin (DHSB ...
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bioRxiv - Microbiology 2022Quote: Plastic tissue culture coverslips were coated with normal mouse serum (50% diluted in DPBS with CaCl2 and MgCl2) (Sigma) for 1 hr at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... On selected sections, immunoreactivities were tested: SATB1 (mouse, 1:500, SantaCruz) and GluR 2/3 (rabbit, 1:500, Millipore) were diluted in 0.1 M PB and incubated overnight at room temperature ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Binding of cGAS and dsDNA in human and mouse brain sections was determined by PLA kit (DUO92101, Sigma-Aldrich) accordingly to the manufacture’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... followed by 4x 8 min in PBS and then incubated in PBS-5% dried semi-skimmed milk with 1:10,000 anti-mouse HRP-linked secondary antibody (A9044, Sigma) for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: Measurement of secretory IgA (SIgA) in mouse faeces samples after treatment with the ONECUT2 inhibitor CSRM617 Hydrochloride (Sigma-Aldrich) or PBS control was done using the SIgA Mouse Uncoated ELISA kit (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: Lentiviral knockdown vectors targeting mouse prkcb and non-targeting control (SCR) were purchased from Sigma-Aldrich (St. Louis, MO) in the pLKO.1 backbone ...
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bioRxiv - Cell Biology 2022Quote: C2C12 mouse myoblasts were obtained from RIKEN BRC Cell Bank and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich) supplemented with 10 % (v/v ...
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bioRxiv - Plant Biology 2022Quote: ... and αTubulin levels were used as loading control (α_Tubulin antibody, Monoclonal Anti-α-Tubulin antibody produced in mouse, Sigma, Catalog number T5168 ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Physiology 2022Quote: ... Primary FSP1 mouse antibody (1:1000 dilution, Cat # 07-2274, 12 kDa) was purchased from EMD Millipore (Burlington, MA). Primary αSMA rabbit polyclonal antibody (1:500 dilution ...
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bioRxiv - Molecular Biology 2022Quote: Duolink™ proximity ligation assays were performed with the Duolink In Situ Red Starter Kit Mouse/Goat (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used for immunoblotting: β-Actin (Mouse monoclonal; A5441; AC-15 clone; Lot# 127M4866V; Sigma-Aldrich), Rsad2 (Mouse monoclonal custom made ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatants were analyzed by western blotting with mouse monoclonal antibodies specific for KRAS (1:1000, WH0003845MI; Sigma-Aldrich) and GAPDH (1:20000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The supernatants were analyzed by western blotting using mouse monoclonal antibodies specific to KRAS (1:1000, WH0003845MI; Sigma-Aldrich) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 3-5 times with 1x TBST for a total of 20 min and then incubated for an hour with 1:3,000 dilution of secondary antibody (goat anti-mouse alkaline phosphatase conjugate, Sigma). The membrane was washed again with 1x TBST 3-5 times for a total of 20 min and developed using 5-bromo-4-chloro-3-indolylphosphate (BCIP)/ nitro-blue tetrazolium (NBT ...
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bioRxiv - Microbiology 2022Quote: ... After incubation at room temperature for 1 hr with a 1:2,500 dilution of horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Sigma), foci were detected by addition of TrueBlue substrate (KPL) ...
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bioRxiv - Molecular Biology 2022Quote: ... cells of Mus musculus (mouse neuroblasts; ATCC, CLC-131 ™) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) – high glucose (Sigma) supplemented with sterile-filtered 10% fetal bovine serum ...
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bioRxiv - Pathology 2022Quote: ... and incubated in 1:100 mouse anti-cytokeratin CAM5.2 monoclonal antibody (item 452M-94,Sigma-Aldrich, St. Louis, MO) or 1:1000 anti- CD163 monoclonal antibody (Novus Biologicals ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were washed and then treated with a 1:1000 dilution of mouse anti-FLAG antibody (clone M2, Sigma) for 60 minutes then washed 3x in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used in this study are: anti-LINE1ORF1p mouse monoclonal antibody (clone 4H1, Sigma; MABC1152; 1:400 dilution); anti-G3BP1 rabbit polyclonal antibody (ThermoFisher ...
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bioRxiv - Plant Biology 2022Quote: ... as well as commercially available mouse anti-alpha-tubulin (Sigma-Aldrich, USA, cat. no. T9026-2, dilution 1:300), rabbit anti-histone H3K4me2 (abcam ...