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Citations for Millipore Sigma :
9651 - 9700 of 10000+ citations for Recombinant Mouse Efna1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: Mouse embryonic stem cells (ESCs) were grown on 0.1% gelatin-coated culture dishes in Glasgow Minimum Essential Medium (Sigma) supplemented with 20% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 48 h at 4°C with either a mouse anti-α-tubulin (DM1A; Sigma-Aldrich) primary antibody at 1:5000 in PBT ...
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bioRxiv - Cell Biology 2024Quote: ... Incubation with primary mouse monoclonal anti-TFR1 antibody (clone 3B82A1, catalog# MABS1982, lot# 3519825, EMD Millipore, Burlington, MA, USA) diluted 1:250 with 0.2% BSA ...
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bioRxiv - Biochemistry 2024Quote: ... anti-p-Histone H2A.X Ser139 (ψH2A.X, mouse monoclonal, 05-636) and pan-ADP-ribose binding reagent (rabbit Fc tag, MABE1016) were obtained from Millipore. anti-DHFR (rabbit polyclonal ...
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bioRxiv - Biochemistry 2024Quote: His6-tagged CCT domains (mouse SPAK 459-556 and human OSR1 433-527) and mouse SPAK 62-556 T243E were expressed in Rosetta (DE3) E.coli (Novagen). Cells were grown at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The following primary antibodies were used: GFP (rabbit, in-house, 1:1,000) and Tubulin (mouse monoclonal, 1:8,000; Sigma-Aldrich). As secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated in a solution containing mouse monoclonal anti-BrdU antibody (1:250, Sigma, Cat.no. B8434) and rabbit polyclonal anti-Doublecortin (DCX ...
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bioRxiv - Cell Biology 2024Quote: ... we use primary anti-acetylated tubulin antibody from mouse (monoclonal 6-11 B-1, T7451 Sigma, 1/1000 dilution) as well as primary anti-tyrosinated tubulin antibody from mouse (monoclonal TUB-1A2 ...
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bioRxiv - Cancer Biology 2024Quote: The following primary antibodies and dilutions were used: mouse-anti-γH2AXSer139 1:1,000 (#05-636, clone JBW301, Merck Millipore) and rabbit-anti-53BP1 1:1,000 (NB#100-304 ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated for 1 hour with the appropriate peroxidase-conjugated anti-mouse secondary antibody at 1/5,000 (EMD Millipore). Immunoreactive proteins were visualized using the LumiGLO chemiluminescent substrate (Cell Signaling).
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bioRxiv - Molecular Biology 2024Quote: ... with two PBS washes after each incubation: primary antibody solution for 1 hour (Mouse Anti-phospho-Histone H2A.X Ser139 Antibody, clone JBW301, Sigma #05-636 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 x in TBS-Tween (0.1%) + 2% BSA before incubation in TRITC conjugated anti-Mouse secondary antibody (Sigma) at 1:1000 concentration for 1 hr in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... The blocking solution was then replaced with another consisting of 2 µL mouse anti-NeuN antibody (Merck Millipore, MAB377) and 0.5 µL of rabbit anti-GFAP antibody (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 12 hr at 4°C in the presence of mouse anti-FLAG (1:1000, Sigma #F1804) and rabbit anti-CD40 (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were tested on the Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore, Cat No. MCYTOMAG-70K-PMX) according to the kit protocol as qualified ...
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bioRxiv - Cell Biology 2024Quote: ... anti-N protein (1:1000, CiteAb #40588-T62) and mouse anti-acetylated α-tubulin (1:200, Sigma-Aldrich #T7451). The secondary antibodies used were goat anti-mouse and goat anti-rabbit conjugated to Alexa Fluor 568 or 488 (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: Primary antibodies used in this study include: Phospho Histone H3 Ser10 Mitosis Marker mouse 1:500 (Millipore 06-570), MMP1 catalytic domain mouse 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... then incubated for 1 hr with an goat anti-mouse HRP-antibody (EMD Millipore, #12-349, diluted 1:10,000). After three more washes with HRP wash ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed thrice with TBS-T buffer prior to addition of horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in blocking solution (anti-mouse-HRP 1:10,000 or anti-rabbit-HRP 1:10,000 antibodies; all from Sigma Aldrich) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were isolated from a single mouse hemisphere in 2 ml of pre-chilled EZ PREP lysis buffer (NUC-101, Sigma) using a glass Dounce tissue grinder (D8938 ...
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bioRxiv - Microbiology 2021Quote: Serum concentrations of Metabolic Panel (Ghrelin, Glucagon, Insulin and Leptin) were determined using the Mouse Metabolic Magnetic Bead Panel Milliplex MAP kit (Millipore).
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bioRxiv - Microbiology 2021Quote: ... IgE) and IgA in feces supernatant were determined using the Mouse Immunoglobulin Isotyping Magnetic Bead Panel Milliplex MAP kit (Millipore) and Mouse IgE Single Plex Magnetic Bead Milliplex MAP kit (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were stained with primary (1:1000, guinea pig anti-NeuN, Millipore #ABN90P; 1:1000, mouse anti-NeuN, Millipore #MAB377) and secondary antibodies (1:400 ...
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bioRxiv - Systems Biology 2020Quote: ... mouse and naked mole rat fibroblasts were cultured as described earlier and treated with varying concentrations of AZC (Sigma-Aldrich). After 5 days of treatment ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared by centrifugation at 8000 g for 10 min and pre-cleared by pre-incubated with 100 μl Mouse IgG−Agarose beads (A0919 Sigma) without antibody for 2 h at 4°C on wheel ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μl of Protein G magnetic beads were used and 10 μg of mouse monoclonal anti-FLAG primary antibody M2 (Sigma) were added before overnight incubation at 4°C with the lysate ...
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bioRxiv - Cancer Biology 2021Quote: ... To deplete mouse STK3 expression in HMVP2 cells TRC2 MISSION shRNAs in pLKO.1-puro vector backbone were used (Millipore-Sigma). Lentivirus was generated with psPAX2 and pMD2.G packaging plasmids using with the Polyplus jetPRIME transfection kit in 293T cells ...
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bioRxiv - Cell Biology 2020Quote: ... or 10μg of p53 antibody (Santa Cruz, DO1) (rabbit or mouse IgG was used as a negative control (Sigma, M7023)) for 4 hours at room temperature ...
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bioRxiv - Cell Biology 2019Quote: Samples were assayed for insulin or proinsulin content using ELISA according to the instructions of the manufacturers (Rat/Mouse insulin ELISA, Millipore, #EZRMI-13K ...
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bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 was used in all experiments except where indicated (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... IRDye 800nm CW Goat anti-Mouse (Li-Cor) at a 1:10,000 dilution for Muc5b or rabbit antilectin UEA-1 (Sigma, U4754) at a 1:1,000 dilution in blocking buffer for Muc5ac was added to the mucin blot and incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... A tertiary streptavidin-HRP antibody (a part of the Mouse ExtrAvidin® Peroxidase Staining Kit from Sigma at 1:1000) was added for 2 hr ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies were detected by incubation for 1 h with FITC-conjugated goat anti-mouse IgG (1:40, Sigma F8264) or Alexa Fluor 568-conjugated goat anti-mouse IgG (1:300 ...
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bioRxiv - Genetics 2021Quote: ... all diluted in a 1:1 mixture of PBT and 3% BSA: mouse anti-α-tubulin (1:500, Sigma T6199), rabbit anti-Spd-2 (1:4.000 ...
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bioRxiv - Immunology 2020Quote: ... fumigatus 12 or 48 h prior were analyzed for cytokines and chemokines using Milliplex Mouse Cytokine & Chemokine 32-plex (Millipore). Plates were read using a BioPlex 200 (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The following primary antibodies and antisera and dilutions were used: mouse anti-tubulin antibody (1:250, Millipore Sigma, cat# T5168); mouse anti-CSP (clone 2A10 ...
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bioRxiv - Microbiology 2021Quote: ... The primary antibodies (monoclonal α-HA rat 3F10 and monoclonal α-myc mouse 9E10, from Roche, distributed by Sigma Aldrich) were diluted 1:1000 and 1:500 respectively in PBS + 0.4 % BSA and the samples incubated for 1 hour at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... tissues were incubated in primary antibody (0.15μg/ml of Mouse Anti-BrdU in 5% BSA) (Cat. no. B2531; Sigma-Aldrich, USA) overnight in a moist chamber at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... In the three subsequent boosters the amount of protein used was 25 μg per mouse mixed with incomplete Freund’s adjuvant (Sigma, India). Boosts were given on days 15 and 28 post-priming ...
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bioRxiv - Developmental Biology 2020Quote: ... were washed in IP buffer and incubated with primary antibody (rabbit anti-HA, anti-myc, CST or mouse anti-flag, Sigma) or normal IgG as control (mouse IgG ...
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bioRxiv - Developmental Biology 2022Quote: Mouse was orally administrated with 15 mg fluorescein isothiocyanate isomer-dextran 4 kDa (FD4, Sigma-Aldrich, St. Louis, MO, USA), serum was collected 4 hours later ...
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bioRxiv - Neuroscience 2022Quote: ... in primary antibodies for 24 hours at 4°C [Primary antibodies: Mouse anti-tyrosine hydroxylase (TH) clone LNC1 (1:500; Millipore) and rabbit anti-vesicular monoamine transporter 2 (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... Membranes were subsequently incubated overnight at 4 °C with primary antibodies (1:2,000 monoclonal mouse anti-TRPA1 antibody, Sigma-Aldrich, and 1:3,000 MF20 mouse anti-myosin heavy chain antibody ...
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bioRxiv - Neuroscience 2021Quote: ... One set of slices were stained with primary antibodies for mouse monoclonal anti-GFAP (G3893; Sigma-Aldrich; 1:1,000 dilution) and rabbit anti-Iba1 (019-19741 ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were stained overnight at 4°C with primary antibody (Mouse anti-γH2AX antibody; Millipore Sigma #05-636, 1:300) diluted in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: The primary polyclonal antibodies applied overnight at 4 °C were as follows: mouse anti-UBE3A 1: 600 (SAB1404508, Sigma, USA); rabbit anti-ELAVL4/HudD 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... antibodies, and the secondary antibodies used included anti-rabbit (31460, Pierce), anti-mouse (31430, Pierce) and anti-rat (A9037, Sigma) HPR antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Normal rabbit IgG (12–370) and Normal mouse IgG (12–371) for ChIP were purchased from Millipore (Billerica, Massachusetts, USA), β-ACTIN (VMA00048 ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed thrice with PBS-T followed by addition of peroxidase conjugated goat anti-mouse IgG diluted 15,000 fold and 20,000 fold (Sigma Aldrich, A4416) to RBD and N protein coated plates respectively ...