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Citations for Millipore Sigma :
9601 - 9650 of 10000+ citations for Recombinant Mouse Efna1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The secondary horseradish peroxidase (HRP)-conjugated antibodies directed against rabbit or mouse were used at a 1/20,000 dilution (Sigma). Detection of luminescent signal was performed with a Luminata Western HRP substrate kit (Millipore).
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bioRxiv - Microbiology 2023Quote: ... This was followed by probing with secondary HRP conjugated anti-mouse or anti-rabbit antibody (1:2000 dilution, SIGMA). Proteins were visualized using the Clarity Western ECL substrate (Bio-Rad 1705060EM ...
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bioRxiv - Bioengineering 2022Quote: ... and chondroitin sulfate were visualized via staining for anti-collagen type I mouse monoclonal antibodies (Sigma MAB3391, 1:100), anti-collagen II mouse monoclonal antibodies (DSHB II-II6B3 ...
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bioRxiv - Biochemistry 2022Quote: ... They were stained with 1/400 (v/v) of primary antibody mouse anti rhodopsin clone 4D2 (Sigma-Aldrich: MABN15) and allowed to incubate for 30 minutes then washed with PBS containing 15 mM NaN3 and 2% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit or mouse primary antibodies were detected using a horseradish peroxidase-conjugated anti-rabbit IgG (Sigma AP307P, 1:4000) or anti-mouse IgG (Sigma AP308P ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were then probed overnight at 4°C with the following antibodies diluted in 5% BSA-TBST: Mouse anti-FLAG monoclonal antibody (1:10,000 dilution, Cat #F1804, Clone M2, RRID: AB_262044, Sigma Millipore), Mouse anti-Menin monoclonal antibody (1:2,000 dilution ...
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bioRxiv - Biophysics 2023Quote: ... The cells were then incubated in a 1:250 Anti-β-Tubulin mouse monoclonal antibody (Millipore-Sigma, T5293-.2ML)-NGS-PBS solution for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-SUN1 pAb (HPA008346) and mouse anti-β-tubulin mAb (T4026, clone TUB2.1) were also from Sigma-Aldrich. Both of them were used at a dilution of 1:200 for immunofluorescent microscopy and at a dilution of 1:1000∼2000 for western blotting ...
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bioRxiv - Cell Biology 2022Quote: ... for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8, 1:2,500, 05-949) for 6xHIS::NMY-2 detection ...
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bioRxiv - Biophysics 2023Quote: ... The cells were then incubated in a 1:250 Anti-β-Tubulin mouse monoclonal antibody (Millipore-Sigma, T5293-.2ML)-NGS-PBS solution for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... was visualized using a mouse monoclonal anti-phospho-Histone H2A.X (Ser139) antibody at a 1:200 concentration (05-636; Millipore) followed by an Alexa Fluor®647 conjugated donkey anti-mouse polyclonal antibody at a 1:1000 concentration (ab150111 ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody, A3682, Sigma Aldrich). All three blots were developed for 9.2 s after adding SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Microbiology 2023Quote: ... Coverslips were fixed in 4% PFA and processed as described above using mouse α-FLAG M2 (Sigma; 1:1,000) and rabbit α-GFP (Abcam ...
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bioRxiv - Genomics 2023Quote: The immortalized mouse primary fibroblasts were cultured in Dulbecco’s Modified Eagle medium (DMEM/F-12) supplemented with 10% FBS (Sigma), 1% penicillin–streptomycin (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... samples were incubated with 1µg/mL of secondary antibodies (AlexaFluor647 anti-mouse, ThermoFisher, or Rabbit-Atto647N antirabbit, Sigma-Aldrich) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated overnight with an anti-GFP rabbit antibody (0.5 μg/ml) (Tamamaki et al., 2000) and an anti-Cre recombinase mouse monoclonal antibody (1:1,000; MAB3120, Merck Millipore). After a rinse ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA was extracted from mouse tail tip samples using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR was performed using the REDExtract-N-Amp PCR Readymix (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Pathology 2023Quote: Human or mouse lung tissue slides (7 μm) were fixed in 3.7% formalin (Sigma-Aldrich, St. Louis, MA, USA), permeabilized in 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... Histone 2B and chromatin)] and 2° antibody cocktail [1:1000 a-mouse-Cy3 (cat.no. 715-166-150, Amersham, UK) and 10µg/ml Hoechst 33342 (cat.no. 14533, Sigma-Aldrich, USA)] (69–71) ...
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bioRxiv - Biophysics 2023Quote: ... samples were incubated with the following primary antibodies for 1h: mouse anti-α-Tubulin (1:1000, T5168, Sigma-Aldrich) and rabbit anti-Lamin A (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The proximity ligation assay (PLA) was conducted with Duolink® In Situ Red Starter Kit Mouse/Rabbit (DUO92101, Sigma) following the standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... PMW1021 (scc-1cs) and PMW1025 (coh-1cs) were detected using the mouse monoclonal anti-flag M2 antibody (Sigma F1804). The heat-shock-inducible ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 1 hr at 37°C with mouse anti-beta-Tubulin (#05-661; Millipore; 1:1500) primary antibody diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... or 250 nM Compound 3 were fixed and stained for mouse anti-γ-tubulin 1:500 (Sigma Aldrich, T5326), rabbit anti-KIF18A 1:100 (Bethyl ...
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bioRxiv - Neuroscience 2023Quote: ... They were stained with 1/400 (v/v) of primary antibody mouse anti-rhodopsin clone 4D2 (MABN15, Sigma-Aldrich) and allowed to incubate for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Coverslips with either adult mouse or neonatal rat cardiomyocytes were incubated 1h with anti-α-actinin antibody (A7811, Sigma) at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit IgG horseradish peroxidase (HRP)-linked antibodies (Amersham) and streptavidin HRP-conjugated antibodies (Sigma-Aldrich) were used for ECL detection Reagents (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: The following primary antibodies were used for this study: mouse monoclonal anti synaptophysin (SP) (clone SVP38, 1.4 ug/ml, Sigma), guinea pig polyclonal anti VGlut11 (1:1000 ...
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bioRxiv - Physiology 2023Quote: ... antibodies were purchased from Developmental Studies Hybridoma Bank. Mouse anti β-ACTIN (cat. A5316, RRID: AB_476743; 1:1,000 for IB) was purchased from Sigma. Goat anti-Mouse IgM (Heavy chain ...
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bioRxiv - Immunology 2023Quote: ... and littermate controls were injected with 0.5cc (0.1mg/mouse) of 0.2mg/ml Zymosan (ZYMOSAN A from Saccharomyces cerevisiae, Sigma-Aldrich, Z4250) solution ...
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (Millipore/Sigma-Aldrich # DUO92101-1KT) and following manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by application of a Cy3-conjugated goat anti-mouse antibody (1:250 dilution, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... 1-5 mm3 biopsies from naked mole-rat or shaved mouse back skin were incubated with either: I) dissociation buffer (1.25 mg/ml collagenase I (Sigma), 0.5 mg/ml collagenase II (Worthington) ...
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bioRxiv - Cell Biology 2023Quote: ... or NK92 cells with antibodies against acetylated (Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse, Sigma-Aldrich, Cat# T6793) and detyrosinated (Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-human-Dia2-isoform1 (gift from Véronique Pizon) and GFP-mouse-Dia2-isoform3 (gift from Yosuke Senju) using X-tremeGENETM HP DNA Transfection Reagent (Sigma) according to the manufacturer’s instructions cultured for a total of 2 days ...
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bioRxiv - Genetics 2023Quote: ... and the basal body was stained with mouse anti-gamma tubulin antibody (clone GTU-88 1:5000, Sigma Aldrich). Following overnight incubation at 4°C with primary antibodies diluted in PBS 1X buffer supplemented with 3% BSA and 0.1% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... Tetraspanin and mouse IgG (mIgG) beads were washed in PBS with 0.1% (m/v) bovine serum albumin (BSA) (Sigma) prior to use ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary antibodies used were as follows: 1:2000 goat anti-mouse F(ab’)2 Alexa488 (Sigma-Aldrich, cat. F8521) and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubating with the primary antibody (mouse monoclonal anti-acetylated tubulin, 6-11B-1, 1:1000, Sigma T6793) and then with the secondary antibody (goat anti-mouse IgG-Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG and AgRP primary antibodies were used and diluted in blocking buffer at 1:200 (mouse anti-FLAG, Sigma) and 1:1000 (rabbit anti-AgRP ...
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bioRxiv - Neuroscience 2023Quote: ... CaBP (1:2000, rabbit, CB38, Swant or 1:2000, mouse, #300) and VGLUT2 (1:7000, guinea pig, AB2251, Millipore). The slices were washed 3 times in 1% Triton X-100 in PBS for 5 to 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated for 1 h at RT with secondary AP-conjugated anti-mouse or anti-rabbit antibodies (1:20,000, Sigma), washed three times for 5 min with PBT and once for 15 min in 20 mM Tris-HCl (pH 9.8 ...
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were performed using the DuoLink In Situ Red Starter Kit Mouse/Rabbit (Millipore/Sigma-Aldrich # DUO92101-1KT) and following manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... in -20°C methanol for 10 minutesCells were stained with mouse anti-γ-tubulin 1:500 (Sigma Aldrich, T5326), rabbit anti-KIF18A 1:100 (Bethyl ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed in PBS-T (0.05% Tween-20 in PBS) and PBS before incubation with 1:10,000 dilution of HRP-conjugated anti-mouse IgG (Sigma) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit polyclonal anti-Laminin (1:50; Santa Cruzcat # sc20142) and mouse monoclonal anti-CSPG (1:200; Sigma cat # C8035). Phalloidin Alexa Fluor 594–conjugated (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 1:500 dilution of rabbit anti-MeCP2 (Cell signalling) and 1:10000 dilution of mouse anti-vinculin (Sigma Aldrich). After the incubation with the primary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubating with the secondary antibodies (anti-Mouse MINUS, DU092004, Sigma-Aldrich; anti-Rabbit PLUS, DUO92002, Sigma-Aldrich) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... One hundred microliters of the E16.5 mouse brain lysate were digested with 1 unit of hyaluronidase from Streptomyces hyalurolyticus (Sigma) at 37L for 1 h ...