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Citations for Millipore Sigma :
9351 - 9400 of 10000+ citations for Recombinant Mouse Efna1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... stained with an Alexa 488-conjugated anti-GFP antibody (Thermo) and anti-Tuj-1 (mouse, 1:1000, Sigma), and imaged as described above ...
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bioRxiv - Pathology 2024Quote: ... 0.5% Triton and lysates were incubated with rabbit or mouse isotypic control antibodies and G-protein beads (Sigma) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The assay was performed using a commercial kit (Duolink In Situ Red Starter kit Mouse/Rabbit, #DUO92101, Sigma-Aldrich) following the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-mouse RIPK3 (clone 8G7; produced in-house5 and available from Millipore as MABC1595; 1:2000/1:400), rat anti-mouse RIPK3 (clone 1H12 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532 ...
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bioRxiv - Cell Biology 2020Quote: Proteins tagged with HA (Mps2-3HA and 3HA-Mps2) were detected by an anti-HA mouse monoclonal antibody (1:1,000 dilution, 12CA5; Sigma). Similarly ...
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bioRxiv - Cell Biology 2020Quote: The following commercial primary antibodies were used throughout this study: Mouse monoclonal antibodies against Actin (clone C4 MAB1501R, Millipore), Fibrillarin (ab18380 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal anti-α-tubulin (1:200 for IF and 1:2500 or 1:10,000 for IB; Sigma-Aldrich); rabbit polyclonal anti-CALK (1:10000 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-Flag used at 1:5,000 (Western blot) and mouse anti-tubulin used at 1:5,000 (Western blot) were obtained from Sigma-Aldrich. Mouse anti-GFP used at 1:100 (immunofluorescence ...
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Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis IIbioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-PP2A C subunit clone 1D6 Alexa Fluor 488 conjugate (Sigma-Aldrich, 05-421-AF488, 1:50), polyclonal rabbit anti-Sgo2 antibody (gift from José Luis Barbero ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA for genotyping was isolated from mouse tail snips with lysis buffer containing Proteinase K (Sigma-Aldrich, P2308). qPCR was performed using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Immunology 2021Quote: ... The same volumes of PBS and LPS (20 μg/mouse, from E. coli O114:B4, Sigma-Aldrich, Shanghai, China) were used as negative control and positive control ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-Neurofilament (cat. #RT97, DSHB, 1:200) nuclei were labeled with DAPI (cat. #D9542, Sigma Aldrich, 1:1000). Images were captured using LSM 510 META ...
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bioRxiv - Developmental Biology 2021Quote: Whole embryonic mouse brains were harvested at E15.5 and lysed in NP-40 lysis buffer with protease inhibitor cocktail (Sigma). Whole brain lysates from one embryo were added to either 6μg GST or 6μg GST-MLLT11 bead slurries and nutated overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The antigen-antibody complexes were visualized using a secondary horseradish peroxidase-conjugated goat anti-mouse IgM antibody (Sigma-Aldrich) at a dilution of 1:10,000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were stained with anti-Rhodopsin rabbit and mouse antibodies (1:200, home-made and 1:200, Millipore, #MABN15), anti-Zpr1 mouse antibody (1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... Several antibodies were used in this study (dilutions are between brackets): Mouse IgG1 anti-Brn3a (Millipore Cat# MAB1585 RRID:AB_94166) (1:250) ...
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bioRxiv - Neuroscience 2022Quote: The resulting concentrate was analyzed using the Milliplex 32-plex Mouse Cytokine/Chemokine Panel (Millipore Sigma MCYTMAG-70K-Px32) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Sections were washed in 0.01M PBS and then placed in primary antibody (NeuN monoclonal mouse anti-neuronal nuclear protein, Millipore MAB377 ...
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bioRxiv - Neuroscience 2021Quote: ... Kyoto University) and mouse anti-glutamic acid decarboxylase of 67 kDa (GAD67; 2 μg/mL; MAB5406, Millipore, RRID: AB_2278725) in Triton-PBS containing 1% v/v donkey serum (Jackson Immunoresearch ...
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bioRxiv - Molecular Biology 2021Quote: Hamster and mouse tissues were homogenized mechanically in RIPA lysis buffer supplemented with 1x protease inhibitor cocktail set (Millipore) and loaded with SDS dye ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with 5% milk in PBS-T and then blotted with a mouse anti-HTT (MAB2166. dilution: 1:5,000; Millipore), rabbit anti β--catenin (Ab2365 ...
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bioRxiv - Immunology 2022Quote: ... The affinity Sepharose resin was prepared by coupling purified rat anti-mouse YN1.1 antibody with CNBr-activated Sepharose 4B (Sigma).
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bioRxiv - Genomics 2020Quote: ... at 33°C for 10min (mouse brain) and 60min 75μl 0.1% pepsin (spleen) prepared at pH 2.5 in Tris-HCl (Sigma-Aldrich, USA). After a 180μl 0.1X SSC wash at 33°C ...
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bioRxiv - Immunology 2019Quote: ... and 5 μm sections were prepared and incubated with a mouse anti-human IgG4 mAb (clone MRQ-44; Sigma) or isotype control ...
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bioRxiv - Microbiology 2019Quote: ... The clarified supernatant was added to 45 μL of equilibrated mouse anti-Flag M2 affinity gel agarose (Sigma-Aldrich). The reaction was incubated overnight at 4°C on a lab rotator ...
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bioRxiv - Developmental Biology 2019Quote: ... sheared chromatin was diluted and incubated overnight at 4°C with the specific antibody against mouse Zic2 (Millipore, AB15392) or IgG isotype control pre-immune serum (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then labelled with different sets of primary and secondary antibodies: mouse anti-HA antibody (1:1000, Sigma), mouse anti-FLAG (1:1000) ...
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bioRxiv - Molecular Biology 2019Quote: ... For the first 5 days of remyelination the mice were administered 4-hydroxytamoxifen (4-HT, 1mg/day/mouse, Sigma) as previously described (Baxi ...
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bioRxiv - Neuroscience 2019Quote: ... PV (mouse anti-Parvalbumin monoclonal antibody, 1:3000, Swant) and SOM (rat-anti Somatostatin monoclonal antibody 1:250, Millipore). The following corresponding secondary antibodies were used ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with rabbit anti-mouse IgG-FITC polyclonal antibody at 1:500 (F9137, Sigma, Milan, Italy) in blocking solution at RT for 2h and counterstained with 0.5μg/mL PI at RT for 5 min ...
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bioRxiv - Physiology 2019Quote: ... Membranes were probed with the primary antibodies for puromycin (MABE343 anti-puromycin, clone 12D10 mouse monoclonal, Merck Millipore Limited), phosphorylated-4E-BP1 (Thr37/46) ...
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bioRxiv - Neuroscience 2019Quote: ... For loading control membranes were re-probed with mouse monoclonal antibody against beta-actin (clone AC-15, Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... We routinely used rabbit PLAplus and mouse PLAminus probes as secondary antibodies and the “Duolink Detection reagents Red” (Sigma) for ligation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 50 μl of mouse plasma was prepared with 450 μL of IP Lysis Buffer (Pierce) plus 0.5% protease inhibitor mixture (Sigma), and immunoprecipitated with 10 μg of polyclonal antibody against mouse ApoAI (Novus) ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was transferred onto a PVDF membrane (Immobilon-P) and detected using mouse anti-c-Myc antibody (Millipore Sigma) as a primary (1:5,000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used in this work were: mouse anti-α-tubulin clone B-512 at 1:1500 (Sigma Aldrich), mouse anti-EB1 at 1:500 (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... blotted and immunodetected with a rat anti-CLASP2 or mouse anti-α-tubulin antibodies (clone B-512, Sigma Aldrich). For analysis of recombinant CLASP2 C-term ...
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bioRxiv - Neuroscience 2019Quote: ... live mouse cortical and hippocampal neurons were incubated with rabbit anti-HA (1:1000 dilution; Sigma-Aldrich, Cat #H6908) diluted in conditioned neuronal culture medium for 15 min at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... and incubated again for 1 hour at room temperature with 1:400 mouse β-tubulin antibody (Sigma Aldrich, T5293) in PBS + 1% BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse monoclonal antibodies to: CFTR (clone M3A7; Cat #: 05-583) and SGLT-1 (Cat #: 07-1417) were from Millipore; NKCC1 (SLC12A2 ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal loading of samples was verified by primary monoclonal mouse anti-GAPDH antibodies (clone GAPDH-71.1, Sigma-Aldrich, Belgium).
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Inhibiting the prostaglandin transporter PGT induces non-canonical thermogenesis at thermoneutralitybioRxiv - Physiology 2019Quote: Mouse intestinal permeability was assessed by 4 kDa FITC-Dextran (FD4; Cat# 46944, Sigma-Aldrich, St. Louis, MO, USA) as previously reported 80 ...
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bioRxiv - Genomics 2019Quote: ... the blocking solution was aspirated and primary antibodies were added (mouse anti-GFP antibodies [Sigma] at 1:100 dilution). After incubation for 1 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: Rds2-FLAG was immunoprecipitated from glucose-treated and non-treated cultures using anti-FLAG M2 antibody (mouse mAb, Sigma) and eluted by FLAG peptide elution ...
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bioRxiv - Bioengineering 2019Quote: ... and either α-smooth muscle actin (α-SMA, mouse monoclonal anti-α-SMA clone 1A4, 1:400, Sigma-Aldrich) or rhodamine phalloidin to visualize F-actin (1:600 ...
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bioRxiv - Genomics 2021Quote: ... S9.6-treated or anti-FLAG treated cells were incubated with 1:100 dilution of anti-mouse antibody (Sigma, M7023) in 200µl of dig-wash buffer at room temperature for 1 hour by rotating.
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bioRxiv - Cell Biology 2021Quote: ... PLA was performed according to the manufacturer’s recommendation (Duolink™ In Situ Red Starter Kit Mouse/Rabbit, Sigma-Aldrich). Primary antibodies against GFP (ab13970 ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with peroxidase-coupled secondary IgG antibodies (anti-Rabbit–A0545 or anti-Mouse –A9044, Sigma Aldrich) for 2 h at room temperature ...