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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for Recombinant Mouse Efna1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Antibodies against the following proteins were used at indicated dilutions: anti-acTub (Sigma-Aldrich T7451, mouse 1:2,000), anti-Arl13b (Proteintech 17711-1-AP ...
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bioRxiv - Immunology 2021Quote: ... Multi-analytic profiling beads using Milliplex® Multiplex mouse cytokine/chemokine panel I kit (EMD Millipore, Billerica, MA) according to manufacturer’s instructions were used to analyze chemokines and cytokines profile into BM and LN tissues at different time periods after LPS or PBS administration into WT mice.
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4°C with monoclonal anti-NeuN antibody (1:500; Mouse, MAB377, Merck Millipore), in a 50% dilution of blocking buffer + 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Astrocytes were evaluated by immunostaining for glial fibrillary acidic protein (GFAP; monoclonal mouse anti-GFAP; 1:500; Millipore, Burlington ...
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bioRxiv - Developmental Biology 2020Quote: ... Westerns were probed with affinity purified rabbit anti-Dsh [24] and Actin AC-40 mouse monoclonal (A4700, Sigma). Detection was using SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... We also used primary mouse monoclonal antibodies against anti-α-acetylated tubulin (clone 6-11B-1, Sigma Aldrich) for detecting mitotic spindles as described in Maiorova and Odintsova ...
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bioRxiv - Cell Biology 2021Quote: All mouse stem cells were maintained on gelatin-coated plates in the ESGRO Complete Clonal Grade Medium (Millipore), and then cultured in the M10 medium (High glucose DMEM ...
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bioRxiv - Cell Biology 2020Quote: Rabbit polyclonal anti-GR (Cat:SAB4501309) and mouse monoclonal anti-αSMA (Cat:A5228) antibodies were from Sigma (St Louis, MO). Anti-TGFβR1 (Cat:ab31013 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were washed with 0.1% TBS-T and incubated with 1:10,000 secondary antibody (rabbit anti-mouse or goat anti-rabbit [Sigma]), diluted in blocking solution for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: Mouse of 5-8 weeks of age were injected intraperitoneally (IP) with 1 µg/g of Tunicamycin (Sigma) using 150 mM of D-Glucose as vehicle ...
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bioRxiv - Cell Biology 2021Quote: ... and horseradish-peroxidase-coupled goat anti-mouse- or anti-rabbit-immunoglobulin antibodies (A0168, A0545, Sigma Immunochemicals, 1:5000) in combination with the enhanced chemiluminescence reaction kit (Amersham Pharmacia Biotech).
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bioRxiv - Immunology 2020Quote: ... cells were then incubated with an anti-flag monoclonal (Anti-Flag M2 produced in mouse, Sigma cat# F1804) antibody at 1:1000 in phosphate buffered saline with 3% BSA overnight at 4°C ...
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bioRxiv - Genetics 2020Quote: ... The ES cells were plated on mitomycin C treated mouse embryonic fibroblast cells (Millipore Sigma #PMEF-NL-P1) that are G418 resistant ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with rabbit anti-protein VI 26 or mouse anti-E1A clone M73 (Millipore, 05-599) diluted in blocking buffer (10% goat serum in PBS ...
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bioRxiv - Immunology 2021Quote: Immortalized mouse LECs (Vigl et al., 2011) were maintained on dishes coated with 10 µg/ml fibronectin (Millipore) and 10 µg/ml collagen type-1 (Advanced Biomatrix ...
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bioRxiv - Molecular Biology 2021Quote: ... The P0 mouse tissues were dispersed by 0.0125% (w/v) (for brain) or 0.0625% (w/v) (for kidney) collagenase (Sigma) treatment in DMEM supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... 400 μg protein from the cytosolic lysate was incubated with 5 μg anti-FLAG mouse IgG1 (M2, Sigma) or mouse IgG1 control overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... then permeabilized and stained intracellularly for granzyme B in 2.4G2 supernatant containing 10% normal mouse serum and 0.5% saponin (Sigma). Sample data was acquired on either an LSR II or LSR Fortessa flow cytometer (both from BD Biosciences ...
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bioRxiv - Microbiology 2019Quote: ... followed by incubation with secondary antibody diluted 1/5000 in blocking solution (goat anti-mouse HRP conjugate, SIGMA) for 2 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... The Cre injection site was verified by staining with mouse anti-Cre recombinase monoclonal antibody (Millipore Sigma, MAB3120) and donkey anti-mouse AlexaFluor 647 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2020Quote: ... The presence of ZIKV in infected cells was detected with a mouse anti-flavivirus 4G2 primary antibody (Millipore) and a donkey anti-mouse IgG horseradish peroxidase-conjugated secondary antibody (Jackson Research Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated overnight at 4°C with primary antibody mouse anti-Flag antibody (1:1000, Sigma). After three washes in PBS (5 min each) ...
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bioRxiv - Cell Biology 2021Quote: ... were coated with anti-FLAG by incubating them overnight with 5 μg/mL mouse anti-FLAG (Sigma #F1804) in 200-mM NaHCO3 buffer (pH 9.4 ...
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bioRxiv - Genetics 2020Quote: ... and then incubated overnight at 4 °C in primary antibody (1:100 mouse anti-Bestrophin (Millipore, Cat# MAB5466); 1:100 rabbit anti-ZO-1 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in primary antibody (1:1,000 mouse anti-calbindin, Sigma; 1:500 rabbit anti-DsRed, Clontech; 1:1,000 guinea pig anti-vGluT1, Millipore) solution containing 5% NDS in PBST for 2 overnights at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were diluted to 1:500 (mouse anti-Myod1, BD pharmaceuticals, 554130; rabbit anti-Sox9, Millipore, AB5535), 1:300 (rabbit anti-cleaved caspase 3 ...
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit anti-GFP (2.5 µg/ml) and mouse DM1α (1:500, Sigma-Aldrich T6199), rabbit anti-Kif18B serum (1:2000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then centrifuged and resuspended in diluted Alexa Fluor 647-conjugated mouse anti-γ-H2AX antibody (Millipore, 05-636-AF647 ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were subsequently incubated with primary antibodies (anti-vinculin mouse monoclonal, Sigma-Aldrich, 1:200 in blocking buffer) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... was washed with IP-lysis buffer and incubated with 3 µg of mouse anti-Flag antibody (Sigma, M2) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse monoclonal anti-FLAG (clone M2; F3165) and rabbit polyclonal anti-FLAG (SAB4301135) were obtained from Sigma-Aldrich. Rabbit anti-SKP1 was generated in the Pagano laboratory (Pagan et al ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-vinculin (1:1000;ThermoFisher Sientific mouse anti-α-tubulin (clone B-512; 1:5000; Sigma-Aldrich) were used as primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies and fixatives used in this study are: Mouse anti-Acetylated Tubulin (1:250, PFA, Sigma-Aldrich T7451), Mouse anti-βII Spectrin (1:1000 ...
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bioRxiv - Genetics 2022Quote: Primary antibodies: anti-phospho-H2AX (γ-H2AX) clone JBW301 (1:200, monoclonal mouse, Millipore Sigma catalogue #05-636), anti-53BP1 (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... The nucleolus was identified using 1:100 L1C6 monoclonal antibody and mouse monoclonal anti α-Tubulin (Sigma-Aldrich) for α-Tubulin ...
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bioRxiv - Microbiology 2022Quote: ... as primary and a goat-anti-mouse antibody conjugated to an alkaline phosphatase (dilution 1:10,000; Sigma, A3562) as secondary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then immunolabeled with primary antibodies specific for FLAG (mouse anti-FLAG M2, Sigma, F3165, 1:200), T7 (rabbit anti-T7 ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated overnight at 4 °C with a mouse anti-tyrosine hydroxylase antibody (anti-TH, Sigma, T1299) at 1:500 dilution ...
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bioRxiv - Systems Biology 2023Quote: Cells were prepared for flow cytometry by first performing a blocking step with 5μl mouse serum (Sigma-Aldrich) then cells were resuspended in FACS Buffer containing 0.5% Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: anti-APC mouse monoclonal antibody (1:200; Merck Millipore, Burlington, MA, USA), anti-GFAP-488 mouse monoclonal antibody (1:300 ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-acetylated tubulin (clone 6-11B-1) was Cat# T6793 (RRID:AB_477585) from Sigma-Aldrich (St. Louis, MO). Rabbit anti-GPSM2 Cat# HPA007327 (RRID ...
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bioRxiv - Cancer Biology 2024Quote: The proximity ligation assay was performed using the Duolink In Situ Orange starter kit mouse/rabbit (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: The proximity ligation assay was performed using the Duolink In Situ Orange starter kit mouse/rabbit (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight in PBS-Triton with mouse anti-Tyrosine Hydroxylase (TH, 1:1000, T2928, Sigma-Aldrich) or chicken anti-TH (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were detected with anti-rabbit or anti-mouse IgG secondary antibodies conjugated to HRP (1:80,000; Sigma). Antigen-antibody complexes were visualized using enhanced chemiluminescence substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated overnight at 4 °C with a mouse anti-tyrosine hydroxylase antibody (anti-TH, Sigma, T1299) at 1:500 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... brain from one mouse from each group were removed under anaesthetized condition (by ketamine-xylazine cocktail, Sigma-Aldrich). Mice brains were placed in dimethyl formamide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... were stained using standard staining protocol (primary antibodies: Mouse monoclonal anti-TH [Immunostar Cat#: 22941 RRID:AB_572268], Rat monoclonal anti-DAT [Millipore Cat# ...
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bioRxiv - Cell Biology 2022Quote: Adult (week 8) mice (Cx40-ChR2 and DbhCreERT/Rosa26-tdTomato transgenic mouse model) were injected with Tamoxifen (Sigma) (150 mg/kg ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were incubated with anti-mouse minus and anti-rabbit plus PLA probes (PLA kit from Sigma) at 37°C for 1 hour ...