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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... degradation of V5-AID-fused Brn1 protein was induced by addition of 0.5 mM indole-3-acetic acid (Auxin, Sigma # 13750).
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bioRxiv - Cancer Biology 2019Quote: ... Methanol/chloroform extraction was performed on of protein for each lysate and pellet finally re-dissolved in 100ul of 6M Urea (Sigma), 2M thiourea (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... Bound proteins were separated by SDS-PAGE and transferred to nitrocellulose before incubation with an anti-myc antibody (9E10; Sigma). Blots were revealed with the Odyssey Western Detection System.
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bioRxiv - Systems Biology 2019Quote: ... and searched with MaxQuant 1.5.1.228 against the yeast protein database from Ensembl 89 and the UPS spike-in Fasta file obtained from Sigma-Aldrich.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... aliquots of 15 µg of hippocampal protein were extracted with lysis buffer containing phosphatase and protease inhibitors (Cocktail II, Sigma) and protein concentration was determined by Bradford’s method ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were centrifuged at 20,000 × g for 20 min at 4°C and the total protein lysate was quantified using Bradford Reagent (B6916; Sigma). Then ...
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bioRxiv - Microbiology 2019Quote: Proteins in cell lysates were separated in a 10% SDS-PAGE and transferred to Immobilon NC (Millipore Merck, Darmstadt, Germany) membranes ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Cell Biology 2019Quote: ... antibodies were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 (GE Healthcare Life Sciences).
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bioRxiv - Cancer Biology 2020Quote: ... 3 μg proteins were separated by SDS-PAGE and electrotransferred onto Immobilon®-P transfer membranes (Merck Millipore, MA, USA), blocked with 5% skim milk in Tris-buffered saline with 5% Tween 20 (TBST) ...
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bioRxiv - Developmental Biology 2020Quote: ... HA-tagged or Myc-tagged proteins were isolated with anti-HA agarose or anti-c-Myc Agarose Affinity Gel (Sigma), respectively ...
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bioRxiv - Systems Biology 2020Quote: ... Total proteins from diverse organelles were extracted using a phase-transfer surfactant solution containing 12 mM sodium deoxycholate (Sigma–Aldrich), 12 mM sodium lauroyl sarcosine (MP Biomedical ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.2mg Dynabeads-Protein-A was blocked overnight at 4°C in 1X IP buffer supplemented with 0.5mg ml-1 BSA (Sigma A7906). The antibody-RNA mixture was split into 50µl aliquots on ice and crosslinked with 0.15J cm-2 254nm UV radiation six times ...
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bioRxiv - Cell Biology 2019Quote: ... Protein was transferred to a PVDF membrane (0.2 micron, Immobilon) and blocked in 5% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 20 mins ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 4°C for 10 min at 20.000xg and supernatant further cleared by incubation with Easy view Protein G agarose beads (Sigma-Aldrich) for 30 min at 4°C (only for FLAG IP) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The MeRIP assay was carried out using the RNA-Binding Protein Immunoprecipitation Kit (Cat# 17-701, EZ-Magna, Millipore, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of protein extracts was performed using a monoclonal anti-Flag antibody covalently attached to agarose (Anti-FLAG M2 Affinity gel, Sigma). Proteins were eluted in 2xNuPAGE LDS Sample Buffer (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of protein or IP pulldown samples were resolved by SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) microporous membrane (Millipore), blocked with 5% skim milk in TBS containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Immunology 2020Quote: ... 10-15 μg of proteins were loaded on to a 96-well plate with a polyvinylidene fluoride (PVDF) membrane at the bottom (Millipore-Sigma), which had been previously activated and primed ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were eluted using 500 μl of IP Buffer containing 0.25 mg/ml of the 3×Flag peptide (Sigma-Aldrich) and 1× cOmplete EDTA-free for 15 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... were expressed as fusion proteins with 6x polyHis tail at the C-terminus in E.coli strain BL21(DE3) (Merck-Novagen, Darmstadt). After harvesting ...
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bioRxiv - Microbiology 2021Quote: ... 30 μl of the purified NtrX protein solution (200 ng/μl) was incubated with 20 μl of 100 mM acetyl phosphate (Sigma) in 50 μl of 2X buffer (100 mM Tris-HCl pH 7.6 ...
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bioRxiv - Pathology 2020Quote: ... 150 mM NaCl containing the corresponding detergents and bound proteins were eluted three times with 10 μL 0.5 mg/mL c-Myc peptide (Sigma M2435) in the corresponding detergent containing buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein lysates were quantified and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). This was followed by immunoblotting with primary antibodies NFATc1 (SAB2101576 ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through from the His-tag cleavage containing the tag free protein was concentrated to 2 mL using Amicon Ultra-4 30k MWCO (Millipore). The concentrated protein was applied to a HiLoad 16/600 S75pg column (GE Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: Rabbit polyclonal anti-TcHTE antibodies were obtained using the p1TcHTE peptide (amino acid 120 to 171) expressed as a TRX.HIS-fusion protein in the vector pET-32a vector (Novagen®), following same strategy we described previously (23) ...
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bioRxiv - Biochemistry 2020Quote: The gene encoding for the full-length protein of the CNFY C866S variant was cloned into a modified pCOLA Duet-1 vector (Novagen) encoding for an N-terminal Strep-tag II and TEV-protease recognition site (construct ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged protein detection was performed as previously reported [71] using an Anti-FLAG M2-Peroxidase (HRP) antibody (Sigma, A8592) and bands were visualized using an ImageQuant LAS 4000 imager.
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bioRxiv - Microbiology 2021Quote: ... A buffer exchange was performed by centrifugation of the protein preparation through an Amicon Ultra-15 centrifugal filter unit (Millipore) with a 10 KDa molecular weight cutoff and subsequent resuspension in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... equal masses of protein samples (20 µg) were resolved using SDS PAGE and then transferred to Immobilon-P PVDF membrane (Millipore). Recombinant NOCT (64-431 ...
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bioRxiv - Synthetic Biology 2020Quote: Protein expression and secretion was evaluated in cultures of 25 ml of PYE media with 0.02% Antifoam 204 (Sigma-Aldrich) after a 16 hr incubation at 20°C with aeration at 250 rpm from a starting OD600 of 0.02 ...
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bioRxiv - Systems Biology 2020Quote: ... 200 μg of protein was then buffer exchanged with 50 mM ammonium bicarbonate with centricon filters (Millipore, 30 kDa cutoff) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... irradiated 18% protein rodent diet (Teklad Global) alone (control) or supplemented with 0.2% w/w bis(cyclohexanone) oxaldihydrazone (Cuprizone, Sigma-Aldrich) in custom gravity-fed food dispensers for 3 to 6 weeks ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at room temperature with an antibody against mHTT protein (1-82 aa, Millipore, MAB5492 1:500), and either NeuN (Millipore ...
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bioRxiv - Plant Biology 2020Quote: ... Induction of protein expression was done 24 hours post infiltration using either 30 μM Dexamethasone (Sigma-Aldrich; St. Louis, USA) and 0.001% [v/v] Silwet L-77 or 20 μM Estradiol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µg of water-resuspended antibody was used to bind to 30 µl of PureProteome Protein A magnetic beads (Millipore).
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bioRxiv - Plant Biology 2021Quote: ... 2.5 to 40 µg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). AGO1 protein was detected using the anti-AGO1 antibody (rabbit polyclonal ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA). The membranes were then incubated with primary antibodies at 4°C overnight ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was collected and protein content was assayed by SDS-PAGE and colloidal blue staining (Brilliant Blue G-colloidal, Sigma), and protein concentration was assayed by the Bradford method (Bio-Rad protein assay ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Developmental Biology 2019Quote: ... using molecular weight markers (26619, Fermentas) to confirm the expected size of the target proteins and transferred to PVDF membranes (IPVH00010, Millipore). Membranes were washed in TBST buffer (TBS containing 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated on a 10% SDS-PAGE and transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore Co., Bedford, MA). Primary antibody incubation was performed overnight at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: Control pLKO.1-LacZ shRNA and four different lentiviral shRNA pLKO.1 plasmids designed to silence DARPP-32 protein expression were purchased from Sigma. To prepare the lentivirus ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was normalized for all tissues with RIPA buffer and 2x SDS sample buffer containing β-mercaptoethanol (Sigma-Aldrich) was added ...
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bioRxiv - Genomics 2021Quote: ... 5 mM sodium metabisulfite and 1 mM MgCl2) containing 100 μg of purified protein and 100 μM GGPP (Sigma-Aldrich). The reaction mixture was overlaid with 500 μL of pentane (Macklin ...
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... the bound proteins were eluted by incubation with 250 μl of 3 x FLAG peptide solution (200 μg/mL) (Sigma) for 15-20 min twice ...