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Citations for Millipore Sigma :
9451 - 9500 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates containing 50 µg protein were separated by electrophoresis on 8–12% polyacrylamide gels and the separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore, USA) at 200 mA for 2 h ...
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bioRxiv - Immunology 2023Quote: ... for 2h at 4°C and washed four times and precipitated protein were competitively eluted with HA-peptide (100ng/ml; I2149, Sigma).
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bioRxiv - Immunology 2022Quote: ... Purified protein was concentrated and buffer exchanged into 1xDPBS using a 10kDa MWCO Amicon Ultra-15 centrifugal filter (Millipore Sigma), aliquoted ...
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bioRxiv - Physiology 2023Quote: ... Supernatants from cells overexpressing Fc-tagged FGL1 proteins were collected after 5 days and supplemented with protease inhibitor cocktail (Sigma). Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare ...
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bioRxiv - Physiology 2023Quote: ... The proteins were collected using 700 μl elution buffer (20 mM Na2PO4, 300 mM NaCl, 300 mM imidazole) (Sigma Aldrich) and dialyzed against MOPS buffer (30 mM MOPS ...
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bioRxiv - Immunology 2023Quote: ... Non-antigen specific IgG and RBD-specific IgG were isolated from serum and eluted RBD-specific antibodies respectively by protein G beads (Millipore). Purified IgG was denatured and treated with PNGase enzyme (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... 20-40 µg of total protein was separated on 7-10% SDS-PAGE polyacrylamide gels and transferred onto Immobilon PVDF membranes (Millipore). Membranes were blocked using 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... membrane at the bottom (Multiscreen HTS 0.45 μm Hydrophobic High Protein Binding Membrane 96-well Filtration plate (Product No. MSIPS4510; Millipore-Sigma) (65 ...
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bioRxiv - Immunology 2023Quote: ... Protein fractions containing pure Cbl-b protein as confirmed by SDS-PAGE were pooled and concentrated using 10 kDa cutoff spin columns (Millipore), and the final protein concentration determined using the NanoDrop UV-Vis spectrophotometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein quantification was normalized to the total protein for each sample determined after transfer to the membrane using Ponceau S solution (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... Sections were processed with a free-floating section staining protocol for immunofluorescence using unconjugated primary antibodies (βIV-Tubulin, T7941, Sigma‒Aldrich, dilution 1:400; GFAP, glial fibrillary acidic protein, G-A-5, Sigma‒Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mNG (Vanderbilt Antibody and Protein Resource Core, VU527) (1:1000) or anti-ɑ-tubulin (Sigma-Aldrich Cat# T5168, RRID:AB_477579) (1:5000 ...
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bioRxiv - Biophysics 2023Quote: The LAF-1 protein coding sequence was ordered as a gBlock Gene Fragment (TM) and inserted into a pET28a(+) vector from Novagen. The LAF-1 mutants E398A (DAAD ...
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bioRxiv - Molecular Biology 2023Quote: Primary neurons were infected for 24 h then protein was extracted by sonication in RIPA buffer supplemented with Protease Inhibitor Cocktail (Sigma) and Phosphatase Inhibitor Cocktail 2 (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... The lysozyme protein solution was filtered by a 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA). The filtered sample was stored in 1.0 mL aliquots at −45 °C until crystallization experiments were performed ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... The quantity of extracellular vesicles was evaluated by measuring the protein concentrations that were measured using Bicinchoninic Acid Kit (Sigma). Cardiomyocytes or spheroids were treated with pelleted vesicles corresponding to 0.1 mg of proteins per 25 000 cells every two days from day 10 until day 24 after the initiation of differentiation.
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bioRxiv - Developmental Biology 2023Quote: ... The resultant samples were load on SDS-PAGE and subjected to pIMAGO phospho-protein detection kit with fluor-680 (Sigma) detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... and secreted proteins in media were harvested after 120 hours and cleared using a 0.45-µm PVDF vacuum filter (Sigma Millipore). Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amount of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Membranes were blocked for one hour in I-BlockTM (# T2015 ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNPs were produced by complexing a two-component gRNA (Edit-R, Dharmacon Horizon) to Cas9 protein with the addition of a PGA (Sigma) electroporation enhancer ...
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bioRxiv - Neuroscience 2023Quote: ... A260/A280 = 0.6) capsid protein was concentrated using a 100kDa MWCO Amicon® Ultra-15 Centrifugal Filter Unit (EMD Millipore) to a final concentration of 1.4 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... the eluted fractions were analysed using SDS-PAGE and concentrated using 10-kDa (for CslA or GlxA monomers) or 100-kDa (for protein complexes) cut-off centrifugal filters (Millipore).
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bioRxiv - Microbiology 2023Quote: ... Protein samples were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by transfer to PVDF membrane (Millipore). The membrane was blocked with 5 % skim milk in tris-buffered saline with 0.1% Tween20 (TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrophoretically transferred to nitrocellulose membranes (Millipore), and blocked in 5% nonfat dry milk ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and 24 hours later treated with SARS-CoV 2 Spike glycoprotein receptor binding domain protein (ScoV2-RBD, 2.8 μg/mL; Baltymas) with and without a specific mitochondrial superoxide scavenger MitoTempo (10 μM; Sigma-Aldrich) for 24 hours.
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bioRxiv - Molecular Biology 2023Quote: ... the virus-containing supernatant was harvested by gentle pipetting and filtered with a low protein binding syringe filter (0.22 μM, EDM Millipore # SLGV033RB). The virus was frozen at -80 °C for long-term storage or transduced directly into the desired cells.
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bioRxiv - Biochemistry 2023Quote: ... Protein was eluted from the resin by applying 6 mL of wash buffer supplemented with 20 mM reduced glutathione (Sigma).
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bioRxiv - Biochemistry 2023Quote: ... The eluted holo-McyA2-(C-A-PCP) protein was pooled and concentrated to 15 mg/ml by ultrafiltration (Amicon Ultracel-10K, Millipore), then ...
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bioRxiv - Cancer Biology 2023Quote: ... TMT-labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (P3300, Sigma) overnight at 4℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated on SDS-PAGE gels and analyzed by western blotting (anti-FLAG M2, 1:1,000 dilution; Sigma, F1804)
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bioRxiv - Immunology 2023Quote: ... Protein was harvested from cell culture supernatants and purified by binding to a nickel-nitrilotriacetic acid (Ni-NTA) column (Novagen) followed by elution with 1M imidazole containing 0.2% octyl β-D-glucopyranoside ...
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bioRxiv - Cell Biology 2023Quote: ... The protein extracts were cleared by 10-min centrifugation and then were incubated with anti-FLAG agarose beads (Sigma-Aldrich) for 16h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Normalized total proteins were analyzed by electrophoresis in 10% polyacrylamide gels and transferred to PVDF membranes (Millipore, Billerica, MA, USA). Membranes were blocked in 5% skim milk (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... All samples were heat-denatured for 5 min at 100°C and run on a 4-20% Criterion Tris-HCl Protein gel and transferred to PVDF membrane (Millipore). Membranes with muscle tissue homogenates were blocked in 5% non-fat dry milk in TBS/0.2% Triton X-100 and incubated overnight in Tau12 (Millipore Sigma ...
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bioRxiv - Systems Biology 2023Quote: ... was applied to the membrane for 30 min to the protein of interest and with Anti-GFP (Millipore-Sigma, 11814460001) for kinase expression ...
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bioRxiv - Systems Biology 2023Quote: ... was applied to the membrane for 30 min to the protein of interest and with Anti-GFP (Millipore-Sigma, 11814460001) for kinase expression ...
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bioRxiv - Biochemistry 2023Quote: ... N-glycomics of BLCA cell lines was performed similarly after immobilization of membrane protein extracts in a hydrophobic PVDF membrane (MAIPS4510; Millipore). All glycans were permethylated and analysed by reverse phase nanoLC- ESI-MS/MS as previously described by us69 ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Bioengineering 2024Quote: Proteins were isolated from 1E6 cells in complete RIPA buffer with protease and phosphatase inhibitors (Sigma-Aldrich, St. Louis, MO). Total protein was quantified using the Pierce BCA Protein Assay Kit (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... The final protein was concentrated to ∼10 mg ml-1 using a 30 kDa molecular weight cut-off concentrator (Amicon Ultra, Millipore). The concentrated protein was flash-frozen and stored at −80 °C until further use.
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bioRxiv - Biochemistry 2024Quote: ... The nhTMEM16 protein peak was collected and concentrated using a 100 kDa molecular weight cut off concentrator (Amicon Ultra, Millipore). The N-terminal GFP-His10 tag was cleaved overnight with 3C protease before gel filtration chromatography by using a Sepharose 6 Increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed as previously described7 using Protein G Dynabeads (LifeTechnologies) coupled to Myc 4A6 antibody (Merck Millipore, 05-724) or FLAG M2 monoclonal antibody (Sigma ...
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bioRxiv - Cell Biology 2023Quote: Approximately 100 µg protein of each sample was separated by 12% SDS-PAGE and transferred to an Immobilon-PVDF membrane (Merck Millipore). The blot was blocked with 5% non-fat milk in 1× TBST (20 mM Tris base ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... CAC-NIH-NA-02)42–44, or anti-zebrafish Ncam1 antibody (1:100, Institute for Protein Innovation) in PBS containing 10 U/mL Heparin (Sigma), 10% FBS ...
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bioRxiv - Plant Biology 2024Quote: ... Total proteins were extracted using denaturing buffer (100 mM Tris-HCl pH 7.5, 4% SDS (sodium dodecyl sulfate, Sigma-Aldrich), 20% glycerol ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylation of NopTC93S proteins was analyzed by 50μM Zn2+-Phos-tag SDS-PAGE and detected with anti–FLAG (Sigma, F1804) antibodies by immunoblot analysis.
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bioRxiv - Microbiology 2024Quote: ... A total of 1 μg MBP-fusion protein (in 100 μl of 1% NP40) was incubated with 0.5 μg Avicel microcrystalline cellulose (Sigma-Aldrich) for 3 hours at 4°C with rocking ...