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Citations for Millipore Sigma :
9101 - 9150 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with whole-cell extract buffer and the FLAG tagged proteins were immunoprecipitated overnight at 4°C using anti-FLAG-agarose beads (Sigma), as described above ...
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bioRxiv - Microbiology 2021Quote: ... Precleared lysates containing the differently tagged or untagged proteins were mixed in a 1/1 ratio and incubated with anti-FLAG-agarose beads (Sigma) overnight at 4°C to precipitate the FLAG-tagged proteins ...
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bioRxiv - Microbiology 2020Quote: ... The analyzed proteins contained an SHH-tag (Sarkari et al., 2014) and were detected using anti-HA (Sigma-Aldrich, USA) antibodies and anti-mouse IgG-HRP (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Elution of proteins was done by adding 100 µl of binding buffer supplemented with 20 mM IP6-sodium salt (Sigma) and then incubating overnight at 4°C under constant mixing ...
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bioRxiv - Microbiology 2020Quote: ... the bound proteins were eluted with 400 μL of wash buffer supplemented with 250 μg/mL HA peptide (Sigma-Aldrich) by incubation at room temperature for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of 20 to 30 μg of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... and total protein concentration was determined using the Bradford Assay according to the manufacturer’s instructions (Sigma-Aldrich, St Louis, MO). Immunoprecipitations were carried out by incubating cell lysates with antibodies as indicated for 12 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... protein of each sample was separated with 12% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene difluoride membranes (Millipore). After blocked by 5% fat-free milk (Sangon Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... antibody-bound fraction was immunoprecipitated for 90 minutes at 4°C using 25 µL slurry salmon sperm protein A agarose beads (Millipore). Unbound proteins were removed by washing the beads 5 times in wash buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Cell Biology 2020Quote: ... treated cells were washed with PBS and collected into RIPA buffer (Fujifilm Wako Pure Chemical) containing protein inhibitor (Sigma-Aldrich). The cells were incubated on ice for 30 min and then homogenized ...
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bioRxiv - Cell Biology 2020Quote: ... the codon optimized gene encoding for rPMDs was subcloned into PET28a (+) and protein expression in E.coli cells was induced with 1 mM isopropyl-β-D-thiogalactoside (IPTG) (Sigma). The his-tagged proteins were purified using Ni-NTA chromatography and the concentration was determined using the BCA estimation kit (Pierce).
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bioRxiv - Biophysics 2021Quote: 5−10 μg of proteins were separated on 10% SDS-PAGE and were consecutively electro blotted onto 0.45 µm nitrocellulose membrane (Millipore, USA). The membrane was blocked with 5% non-fat skim milk in PBS-0.1% Tween 20 for 2 hours at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... For ADH/DMTMM reactions, protein samples were incubated with 57mM ADH (d0/d8, Creative Molecules) and 36mM DMTMM (Sigma-Aldrich) for 15 minutes at 37°C while shaking at 350 rpm ...
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bioRxiv - Biophysics 2021Quote: All proteins were concentrated using ultrafiltration concentrators with either 3 kDa or 30 kDa MWCO regenerated cellulose membrane (EMD Millipore), flash frozen in small aliquots in liquid nitrogen ...
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bioRxiv - Biophysics 2020Quote: Wild-type or Y473E mutant human PPARγ LBD (UniProt P37231; residues 203–477, isoform 1 numbering) protein was expressed from a pET46 Ek/LIC vector (Novagen) as a Tobacco Etch Virus (TEV)-cleavable N-terminal Hexa(6x)His-tagged fusion protein in Escherichia coli BL21(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... and the dried protein pellet resuspended in digestion buffer (100 mM Tris-HCl pH 8.5, 1% sodium deoxycholate (Sigma-Aldrich), 5 mM TCEP ...
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bioRxiv - Cell Biology 2021Quote: Equal amount of protein lysates was loaded and resolved on SDS-PAGE gel and transferred onto an PVDF membrane (Millipore). Blots were blocked with 5% BSA for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... + 10 mM dithiothreitol to reduce the proteins and then for 30 min at room temperature with 50 mM NH4HCO3 + 50 mM Iodoacetamide (SIGMA) in the dark to alkylate them ...
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bioRxiv - Cancer Biology 2021Quote: ... Methanol/chloroform extraction was performed on 100ug of protein for each lysate and pellet finally re-dissolved in 100ul of 6M Urea (Sigma), 2M thiourea (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to determine the concentration of harvested cell lysates and 500 μg of protein lysate was loaded into the supplied spin column (Catch and Release Immunoprecipitation Kit; Millipore). Immunoprecipitation using antibodies against FLAG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was resuspended in digest buffer (0.1 M triethylammonium bicarbonate, pH 8.5, Sigma-Aldrich, tandem mass tag (TMT) labeling using a 6-plex TMT kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested DNA was then incubated with 50 µl of protein A/G agarose beads and 5 µl anti-S9.6 (Millipore, MABE1095) overnight at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... Some samples that were used to obtain or confirm resonance assignments were assembled by mixing protein with equal volume of 1.5 M ammonium sulfate (Sigma-Aldrich), 0.1 M Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10 μg of proteins were separated by electrophoresis in an 10% SDS polyacrylamide gel and transferred onto a polyvinylidene fluoride membrane (PVDF, Millipore). The membranes were blocked with 5% (v/v ...
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bioRxiv - Biophysics 2022Quote: ... The purified protein was concentrated up to 5 mg/mL using Centricon YM-10 and Microcon YM-10 concentrators (Amicon, Millipore). Crystals used in this study were obtained under oxygen exclusion in hanging drops from 0.5 M Hepes pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was then further purified by concentrating and running over a gel filtration column (CYZ superdex 200 increase, Sigma) in 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were washed three times with 0.5 ml of FLAG wash buffer and the bound protein was eluted with 1X SDS-PAGE loading buffer supplemented with 100 µg·ml-1 3X FLAG peptide (Sigma-Aldrich) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... PKG Iβ (71-686) protein was concentrated to 20 mg ml-1 by using 30 kDa cutoff Amicon Ultra (Millipore) and initial crystal screening was performed by Mosquito Crystal robot (TTP Labtech ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated using a prewashed Amicon Ultra-10 concentrator (molecular weight cut off 30 kDa; EMD Millipore), flash frozen in liquid N2 and stored in -80°C.
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bioRxiv - Biochemistry 2022Quote: ... The purified protein was concentrated using a prewashed Amicon Ultra-10 concentrator (molecular weight cut off 10 kDa; EMD Millipore), flash frozen in liquid N2 and stored in -80°C.
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bioRxiv - Biochemistry 2022Quote: Purified full-length E-cadherin was concentrated to 8-10 μM and mixed with the nanodisc scaffolding protein MSP1D1 (Sigma) at a 5 fold molar excess ...
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bioRxiv - Neuroscience 2022Quote: Tissues from Tmem150c-/- mutants and wild type mice were lysed with 8 M urea buffer and protein concentrations were determined using the Bradford reagent (Sigma). Proteins were separated by SDS-PAGE ...
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bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Each 15 μg sample of protein was separated using 8% SDS polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to a nitrocellulose membrane (Sigma). The membrane was blocked with 5% not-fat milk in 0.1M Tris-buffered saline (pH 7.4)/Tween 0,1% (TBSTween ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were resolved by SDS PAGE and transferred to an Immobilon P polyvinylidene fluoride or Immobilon-FL membrane (EMD Millipore)64 ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were centrifuged 15 min at 13200 rpm at 4°C and protein concentration measured by Bradford assay (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... The cells were sonicated on a Sonics Vibracell VCX750 Ultrasonic Cell Disrupter (Labotal) and BugBuster protein extraction reagent (1:100 ratio) (Novagen) were added to complete the lysis ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was collected from the column and concentrated approximately 10-fold using an Amicon Ultra Centrifugal Filter Unit (Millipore) with a 3,000 Da cutoff ...
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bioRxiv - Biophysics 2022Quote: ... the proteins were expressed in Escherichia coli strain BL21 Star (DE3) cells using 0.4 mM Isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma) for 9 to12 hours at 20 °C ...
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bioRxiv - Biophysics 2022Quote: ... Protein was concentrated to 30-40 mg ml-1 using a 50-kDa cutoff Amicon Ultra centrifugal filter unit (Millipore). NpSxph crystals were obtained by hanging drop vapor diffusion at 4°C using 1:1 v/v ratio of protein and precipitant ...
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bioRxiv - Biochemistry 2022Quote: ... Expression of the SSZ-FTpA protein was verified by Western blotting of whole-cell lysate using PAP (Sigma-Aldrich, P1291) antibodies according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... β-actin protein level was evaluated by western blot analysis of rehybridized membranes with anti-β-actin antibody (Sigma, A5441) at a 1:5000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... The column was washed with 30 column volumes (CV) of wash buffer before bound proteins were eluted with wash buffer supplemented with 3 mM D-desthiobiotin (Sigma). The protein was transferred into wash buffer without D-desthiobiotin and incubated overnight at 4°C with 1:100 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Cell Biology 2022Quote: ... The purified protein was then concentrated to 300 μM using an Amicon-2 Ultra concentrator (Millipore Sigma, product number UFC2030). DTT was added to the concentrated protein to a final concentration of 1 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to the culture media for 8 hours before preparing protein lysates with the above lysis buffer supplemented with N-Ethylmaleimide (25mM, Sigma). Lysates were pre-cleared using Protein G sepharose beads (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed by separation on SDS-PAGE gels (8-12%) and transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore). Immunoblots were imaged using an anti-Ssa1/2 polyclonal antibody at a 1:10,000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... glutathione or flag peptide were removed by buffer exchange and the purified proteins were concentrated on Amicon Ultra 50K filters (Millipore). Purified proteins were recovered in 20 mM Hepes ...
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bioRxiv - Cell Biology 2022Quote: The induction of the unfolded protein response was carried out by the addition of 5 µg/ml tunicamycin (Sigma-Aldrich) in medium and incubation for 6 h prior cell lysis or the supplementation of pre-warmed medium with 10 mM DTT (Sigma-Aldrich ...