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Citations for Millipore Sigma :
9051 - 9100 of 10000+ citations for Recombinant Human CYCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Approximately 5×105 cells stably expressing the indicated TurboID-V5-fusion proteins were treated as described prior to the addition of 500μM biotin (Sigma-Aldrich) for 30min ...
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bioRxiv - Bioengineering 2020Quote: ... For western blotting 40 μg of protein samples were subjected to 10% in house SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 3% BSA in TBS containing 0.01 % Tween-20 for 1 h at room temperature and then incubated with specific primary antibodies at 4 °C overnight in the blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: To expose Prdx2 to continuous H2O2 generation the protein (20 μM) was incubated with 5 mM glucose plus glucose oxidase (Sigma). Glucose oxidase was diluted to produce approximately 0.5 μM H2O2/min.
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bioRxiv - Biochemistry 2020Quote: ... Fractions containing >90% pure protein (evaluated by SDS-PAGE) were concentrated using an Amicon Ultra-15 centrifugal unit (EMD Millipore) and flash frozen in liquid nitrogen before storage at −80°C.
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bioRxiv - Biochemistry 2020Quote: ... Each protein encoding DNA fragment was assembled with the mCherry-PCR fragment and NdeI and HindIII digested pET22b vector (Novagen), using a Gibson assembly reaction (New England Biolabs) ...
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bioRxiv - Biochemistry 2020Quote: A protein/Sypro orange mix containing 5 μM protein and a 1:5,000 dilution of dye in DMSO (as supplied by Sigma Aldrich) was prepared just before plate setup in 20 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant protein concentration was assessed by quantification of the change in absorbance of the Bradford reagent (Sigma-Aldrich, USA) at 595 nm utilizing Infinite F200 PRO multimodal microplate reader (Tecan ...
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bioRxiv - Microbiology 2021Quote: ... The resin was loaded into a column and the flow through containing S-tagged gyrase A was further purified using an S-protein agarose (Sigma) by elution with 20 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were sequentially incubated with primary and HRP-coupled secondary antibodies and proteins were detected using chemiluminescence reagents (Luminata Crescendo, Millipore) and X-ray films ...
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bioRxiv - Neuroscience 2021Quote: ... a total of 4 μg of S protein diluted in 12 μl sterile ddH2O containing hyaluronidase (100 UI; Sigma-Aldrich) were infused in the intranasal cavity of mice ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and nsp12 proteins were subsequently detected using the mouse monoclonal anti-DYKDDDDK M2 antibody (Catalogue number F3165, Sigma-Aldrich) diluted 1:1000 in TBST/5% milk ...
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bioRxiv - Cell Biology 2021Quote: ... 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma) were added to each sample for 3.5 hours at 4°C after the overnight incubation with antibody ...
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bioRxiv - Biophysics 2021Quote: ... The protein was expressed and purified the same way as SARS-CoV Mpro-2 but Factor Xa protease (Sigma, Canada) was used (4 °C ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). For PDAC cells grown as 3D organoids in co-culture ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin was purified from 293T cells engineered to produce it using a Protein A Antibody Purification Kit (Sigma PURE1A). Organoids were grown in complete media when passaging ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein samples were subjected to SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, MA, USA). The membranes were treated with 1% blocking solution in TBS for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... 25 µg of the protein was loaded in 4-12% Bis-Tris gradient polyacrylamide gel and transferred onto PVDF membrane (Millipore). Membranes were blocked in PBS with 0.1% Tween 20 and probed with respective primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein (11 µg) were run on a 10% SDS-PAGE gel and transferred to a polyvinylidene difluoride membrane (Millipore) overnight ...
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bioRxiv - Plant Biology 2021Quote: Total PME activity was quantified on cell wall-enriched protein extracts using commercial citrus pectins (DM >85% P9561, Sigma-Aldrich) and the alcohol oxidase-coupled colorimetric assay (Klavons and Bennet ...
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bioRxiv - Plant Biology 2021Quote: ... Chromatin samples were incubated with 50 ml of washed Protein G Dynabeads pre-coated with 1mg of S9.6 antibody (Millipore MABE1095) for 16h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the protein were pooled and concentrated with an Amicon Ultracell Centrifugal filter unit (MWCO 50 kDa, Merck Millipore) to a volume of 500 µL and digested with TEV protease for 2 hours at 8°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Cell Biology 2021Quote: ... The 400 μl of protein lysates were incubated with 20 μl of anti-FLAG® M2 affinity gel (Millipore-Sigma) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The 400 μl of protein lysates were incubated with 20 μl of anti-FLAG® M2 affinity gel (Millipore-Sigma) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 30-50 micrograms of the protein were separated on a 15% polyacrylamide gel and electrophoretically transferred to PVDF membranes (Millipore). Membranes incubated with the primary antibodies against claudin-5 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Biochemistry 2021Quote: ... Insoluble material was removed by centrifugation at 149,000 ×g for 45 min and the solubilized protein was loaded onto a gravity column of 2 mL M2 anti-FLAG affinity matrix (Sigma). The column was washed with 10 mL of wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: The fractions containing eluted proteins were concentrated to ∼5 ml by AmiconTM ultrafiltration devices (10 kDa or 30 kDa molecular weight cut-off) (Millipore) and centrifuged in a bench centrifuge for 10 min at maximum speed to remove any precipitated material before being subjected to size-exclusion chromatography (SEC) ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was assessed 72 hours post transfection via Western Blot using a monoclonal antibody against the FLAG epitope (Sigma) (Figure 5A ...
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bioRxiv - Microbiology 2020Quote: ... the same samples were incubated for 2.5 h at 37°C with protein synthesis inhibitor cycloheximide (10 μg/ml) (CHX; Sigma-Aldrich), of which experimental condition was optimized in our previous reports 18,21 ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Loaded proteins were washed with 300 μL Binding Buffer (90% methanol and 10% triethylammonium bicarbonate buffer (TEAB, Sigma-Aldrich, #T7408), adjusted to pH 7.1 using phosphoric acid ...
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bioRxiv - Microbiology 2020Quote: Fractions containing the DnaA-6xHis protein were pooled together and applied to Amicon Ultra Centrifugal Filters with 30 kDa cutoff (Millipore). Buffer was exchanged to Buffer A (25 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... Total protein samples (30 μg) were resolved in 12%-SDS-PAGE and transferred to ImmobilonTM PVDF membranes (Millipore, Burlington, MA) in a semi-dry electroblotting apparatus (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... 20-30μg of protein extract was separated on a 4-20% precast polyacrylamide gel and transferred to a PVDF membrane (Millipore). Membranes were blocked with 5% nonfat milk in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2020Quote: ... About 1 mL of lysate with similar protein concentration was added to tubes with 50 μL of anti-FLAG magnetic beads (Sigma) and rotated for 2 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Residual methylation reagents were neutralised by addition of 1M Tris-HCl pH 7.5 to a final concentration of 50 mM Tris-HCl and the protein complex was concentrated to 2 mg mL−1 using 30 kDa cut-off concentrators (Millipore) before injection into a S75 16/600 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by 12% SDS–PAGE gels and detected using immunoblotting with a monoclonal α-Flag antibody (Sigma-Aldrich) or a α-GFP antibody (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... After incubation for 90 min at RT the microplates were washed 4X (PBS-Tween 20) and 25 μl of protein LA-HRP diluted 1:3000 (Sigma) was added and the microplates were then incubated for 1h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Eight micrograms of protein per lane were separated by 12% SDS polyacrylamide gel electrophoresis and blotted onto Immobilon-P nylon membranes (Millipore). After transfer ...
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bioRxiv - Microbiology 2021Quote: ... The protein interlayer solution in urea was then incubated at 60° C after adding dithiothreitol (Sigma Aldrich, St. Louis, MO) at 5 mM concentration for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... lysates of tissues or tells were incubated with antibodies overnight (4°C) and subsequently with protein G-agarose beads (Millipore) for 5h (4 °C) ...
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bioRxiv - Microbiology 2021Quote: ... 5 µg of purified MurA protein were mixed with 10 mM uridine 5′-diphospho-N-acetylglucosamine (UDP-GlcNAc, Sigma-Aldrich) in a reaction buffer containing 100 mM Tris-HCl pH8.0 and 150 mM NaCl (final volume ...
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bioRxiv - Plant Biology 2021Quote: ... the total protein was extracted from tobacco expressing CmTPL1-2-HA and CmSVP-FLAG using anti-HA affinity gel (E6779, Sigma), followed by western blotting with anti-FlAG (A8592 ...
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bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
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bioRxiv - Systems Biology 2020Quote: ... TMT labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (Sigma) overnight at 4 °C ...