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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... was dissolved in isopropanol:chloroform (2:1) and added in small aliquots to a stirring 5% (w/v) methyl-β-cyclodextrin (Sigma-Aldrich) solution in a water bath heated to 80°C ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at room temperature with a combination of 2 different primary antibodies: a mouse monoclonal anti-PCNA antibody (1:500; Sigma-Aldrich; catalogue number P8825 ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
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Complement receptor C5aR1 signaling in sensory neuron-associated macrophages drives neuropathic painbioRxiv - Pharmacology and Toxicology 2022Quote: ... or PSNL mice and incubated in a solution containing 1 ml of DMEM medium and 2 mg/ml of collagenase type II (Sigma-Aldrich) for a 1 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2024Quote: ... 1×10^6 POE SHSY5Ys were reverse transduced with the 1:2 lentivirus supernatant dilution in the presence of 10 µg/ml polybrene (Sigma Aldrich). Bulk populations of cells stably expressing mitoSRAI were established through selection with 1ug/ml puromycin (M P BIOMEDICALS UK) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 8 µL of 300 µM final concentration of substrate diluted in TVMV protease assay buffer (50 mM Tris/HCl, 100 mM NaCl, and 2 mM DTT, 50 mg mL-1 BSA (Sigma-Aldrich), pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... barcode set in suspension using identical concentrations for 2 hours at room temperature before 2x washes with GSH-CSB (1 mM Gulutathione (Sigma #G6529)) ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... The transfection plasmids were mixed in a 4:2:1 ratio in Opti-MEM medium utilizing the polycationic agent polyethylenimine (PEI; Sigma-Aldrich). Following transfection ...
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bioRxiv - Immunology 2024Quote: ... 2 x 106 cells per well were cultured in 200 μL supplemented RPMI 1640 medium and stimulated during 2 h at 37 °C with 50 ng/mL PMA and 1 µg/mL ionomycin (Sigma-Aldrich) in the presence of Brefeldin A and Monensin (eBioscience) ...
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bioRxiv - Microbiology 2024Quote: ... Donor and recipient cells were mixed to a ratio of 10:1 and 2 µL of the mating was spot into a microscope slide containing an M9 (M9 salts (Sigma-Aldrich), 500M mM MgSO4 ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... Reactions were carried out at 42 °C for 1 hr and then treated 5 μmol sodium 2-mercaptoethanesulfonate (MESNa, Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were cleared using a 1:2 mixture of Benzyl Alcohol: Benzyl Benzoate (108006, B6630, Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Developmental Biology 2023Quote: ... samples were dehydrated in series of Methanol and cleared using BABB (a mixture of 1 part benzyl alcohol to 2 parts benzyl benzoate; Sigma-Aldrich, benzyl alcohol ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed in 100% methanol for 1.5 h and cleared in BABB (1:2, benzyl alcohol:benzyl benzoate) (Sigma-Aldrich, 108006, B6630).
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bioRxiv - Immunology 2023Quote: ... 3-4 and 7-9 were treated with 100 µL of embryo water plus 1 µg/mL 2′,7′-Dichlorofluorescein diacetate (H2DCFDA) (Sigma-Aldrich) in 0.4% DMSO ...
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bioRxiv - Paleontology 2023Quote: All aqueous solutions were prepared from ultrapure grade water obtained by water filtration with a two stages Millipore system (Milli-Q® Academic with a cartouches Q-Gard 1 and Progard 2, Merck Millipore, Burlington ...
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bioRxiv - Neuroscience 2023Quote: ... A high-titre solution of viral vector (AAV9-syn-jGCaMP7f-WPRE) was diluted to a final ratio of 2:1 with a 20% mannitol solution (Sigma-Aldrich) to promote diffusion ...
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bioRxiv - Neuroscience 2024Quote: ... the dehydrated tissue was immersed in a BABB solution (benzyl alcohol and benzyl benzoate in a 1:2 ratio; Sigma Aldrich). The cleared ganglia were subsequently mounted between a slide glass and a coverslip ...
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bioRxiv - Cell Biology 2024Quote: ... mucus from injected animals was removed by cooling worms on ice for 1 min then incubating on ice-cold 2% HCl (Sigma H1758) for 3 min ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were plated in serum-free growth medium (SFGM, Supplementary Table 1) on fibronectin-coated (4 μg cm-2 bovine fibronectin; F1141, Sigma-Aldrich) tissue culture vessels ...
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bioRxiv - Bioengineering 2023Quote: To generate emulsions, 1 mL of fluorinated oil (Novec 7500, ACOTA) and 2 mL of protein solution (β-lactoglobulin; >90%, Sigma, from bovine milk ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250). Lysates were separated on 4-12% BoltTM Bis-Tris gels (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... sections were mounted in 3D homemade printed chambers covered with coverslips and sealed with a melted paraffin–vaseline mixture (∼2:1 ratio) (Cat. #: P3558, Sigma-Aldrich. Vaseline pure ...
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bioRxiv - Cancer Biology 2023Quote: ... Stable KD of EIF4G2 was generated by infecting HEC-1A or RL95-2 cells with lentiviruses harboring pLKO.1-puro plasmid expressing shRNA targeting GFP (Control) or shRNA targeting EIF4G2 (Sigma TRCN0000147914), followed by selection using puromycin ...
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bioRxiv - Developmental Biology 2024Quote: ... was performed by incubating the zebrafish for 24 hours with 1 mM (2-month-old fish) or 10 mM (larvae) MTZ (Sigma-Aldrich) diluted in 1% DMSO (VWR ...
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bioRxiv - Biochemistry 2023Quote: Mice were kept under 2% isoflurane-induced anesthesia and the tibialis anterior muscles were injected with 30 µL of 1 mg/mL hyaluronidase (Sigma, #H3506). After 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Both pre-gRNA- and gRNA-based spacers were designed as two separate oligos (Supplementary tables 1-2) and synthesized by Sigma Aldrich. The oligos were annealed ...
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bioRxiv - Bioengineering 2022Quote: ... 0.2 μL of reverse primer (both at 10 μmol L- 1) and 7.6 μL of molecular grade water (Sigma Aldrich, USA). Three qPCR technical replicates were prepared for each sample ...
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bioRxiv - Cell Biology 2022Quote: ... HESC were treated with differential medium (DMEM/F12 with 2% CS-FBS) containing 1 μM Medroxyprogesterone 17-acetate (MPA, Sigma, M1629), and 0.5 mM dibutyryl cAMP (dbcAMP ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250). Lysates were separated on 4-12% Bolt™ Bis-Tris gels (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Newly eclosed adults were kept for an additional 1–2 days before inducing transcription activation by placement on food containing the steroid hormone mifepristone (RU-486; Sigma M8046) in a 25 μg/ml concentration and flipped every 2 days thereafter ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... DOI (1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride-hydrochloride) and serotonin were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). LP44 (4-[2-(methylthio)phenyl]-N-(1,2,3,4-tetrahydro-1-naphthalenyl)-1-piperazinehexanamide hydrochloride ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... (60 mg, 0.23 mmol, 1 eq.), sodium pyruvate (46 mg, 0.345 mmol, 2 eq.) and Neuraminic5Ac aldolase (Sigma Aldrich EC 4.1.3.3) from E ...
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bioRxiv - Systems Biology 2022Quote: ... 50 mL of blocking buffer was prepared per 2 × 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Neuroscience 2022Quote: ... RabiesΔG-mCherry virus (titer of around 110 TU/ml) was mixed at a 2:1 ratio with silk fibroin (Sigma #5154) to make rabies injection solution (Jackman et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... The heart was excised and sectioned in 2-mm slices and then stained with 1% triphenyl tetrazolium chloride (TTC) (Millipore Sigma) in PBS solution at 37°C for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... the mixture was transferred into 15 ml centrifuge tube and 2 ml 1% bovine serum albumin (BSA; Sigma, USA, cat# A4628) was added to terminate dyeing ...
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bioRxiv - Physiology 2023Quote: ... into the new glass vial with a PTFE cap (60940A-2, Fisher) filled with 25 μL of 1 μg/μL (-)-Nicotine (N3876-100ML, Sigma Aldrich), for a total of 25 μg of added nicotine ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
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bioRxiv - Biochemistry 2023Quote: ... to 20 µl of 60S subunits (blasticidin S dataset) and 1 µl of 2 mM water-solubilised cycloheximide (Sigma-Aldrich 01810) to 20 µl of 60S subunits (cycloheximide dataset) ...
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bioRxiv - Genomics 2022Quote: ... Each lobe was dissected off and minced into small pieces in a beaker containing 39 mL SC-2 supplemented with 1 mL DNase I (Sigma-Aldrich) and 20 mg collagenase P ...
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bioRxiv - Cancer Biology 2023Quote: ... and GSC #161) were obtained from the Mayo Clinic [44] and cultured on laminin-coated plates (1–2 μg/cm2) (Sigma #L2020) in StemPro neural stem cell (NSC ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubated in a shaking water bath (180 rpm) at 37°C for 50 minutes with Type 1 Collagenase (2 mg/ml; Sigma-Aldrich) and DNase (100 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The zona free eggs were washed 2× in 1 % BSA in PBS and fixed with 3.7 % paraformaldehyde (P6184, Sigma-Aldrich®) for 20 min and washed 2× in 1% BSA in PBS ...