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Citations for Millipore Sigma :
9101 - 9150 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... G6PD activity was measured with the Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Sigma, MAK015), and uric acid levels in heart lysates were measured with the Uric Acid Assay Kit (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo) containing 143 mM 2-Mercaptoethanol (Sigma-Aldrich). Western blotting using the elutes from affinity purification and the pre-purified input lysates were performed as described above with the following primary antibodies ...
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bioRxiv - Biophysics 2021Quote: HeLa H2B-mCherry day 2 aggregates were equilibrated for 1 h in CO2 independent medium containing 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich) and 1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... For the inhibition of Thymidylate kinase (DTYMK) cells were incubated for 72 h after adding 2 μM (final conc.) of the YMU-1 compound (Sigma-Aldrich), solved in 15 % DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... two CSNK1D targeting shRNA constructs were selected from the Mission TRC shRNA library (TRCN0000023769, named shCSNK1D#1; TRCN0000361946, named shCSNK1D#2; Sigma-Aldrich); non-targeting scrambled shRNA served as control (shc002 ...
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bioRxiv - Cancer Biology 2021Quote: ... prior to lysis in ChIP lysis buffer (20 mM Tris-HCl, 150 mM NaCl2, 2 mM EDTA, 1% IGEPAL CA-630 (Sigma-Aldrich) and 0.3% SDS in water) ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Genetics 2021Quote: ... Telomere length was measured by two orthogonal methods: 1) qPCR8 and 2) telomere restriction fragment (TRF) analysis using TeloTAGGG Telomere Length Assay (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... without L-glutamine and without phenol red (Biochrom) supplemented with 30 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma-Aldrich) with a pH of 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed cells were permeabilized with PBS containing 0.2% Triton X-100 for 10 min and blocked with PBS containing 1% IgG-free bovine serum albumin (BSA) (Sigma, A2058) for 30 min at RT ...
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bioRxiv - Biophysics 2022Quote: ... hiPSC-CMs were maintained in RPMI 1640 with HEPES and Glutamax (Life Tech, Bleiswijk, the Netherlands) with 2% B27 with insulin (Life Tech) and 1% penicillin/streptomycin (P/S, Sigma-Aldrich). Six days before mechanical stimulation ...
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bioRxiv - Physiology 2022Quote: ... 117 NaCl, 10 KCl, 1 MgCl2, 10 creatine, 20 taurine, 5 adenosine, 2 L-carnitine, 10 glucose, 10 HEPES [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2021Quote: ... A crude nuclei suspension was prepared from the homogenate by 1-2 rounds of washing (Nuclei PURE store buffer, Sigma, S9183) and centrifugation (700g for 3 minutes at 4 degrees) ...
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bioRxiv - Molecular Biology 2020Quote: ... the cryosections were incubated with Alexa FluorTM 488 goat-anti-Rabbit IgG (1:1000) for 2 hours at room temperature and then the nuclei counterstained with Hoechst 33342 (Sigma-Aldrich). The cryosections were imaged using the A1+ Confocal Microscope System with NIS-Elements Advanced Research software (Nikon ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was gradually dehydrated in resistant glassware with incremental concentrations of tetrahydrofuran (TFH, 50%, overnight; 80%, 1 h and 100%, 2 h, v/v % in distilled water, Sigma-Aldrich). Incubations were performed on a shaker at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... A refractive index matching solution was prepared by mixing BABB (benzyl alcohol + benzyl benzoate 1:2; Sigma-Aldrich, 24122 and W213802) and DPE (diphenyl ether ...
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bioRxiv - Bioengineering 2021Quote: ... a 95/5 L-lactide/DL-lactide copolymer (Corbion) 2% m v−1 solution in dry 1,4-dioxane (Sigma-Aldrich, 296309) was spin-coated at 3000 rpm for 30 s on 1.25 × 1.25 cm Corning® glass microslides (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... were fluorescently labelled by the addition of 2 μl PKH26 dye (1 mM stock concentration) from the MINI26 PKH26 Red Fluorescent Cell Linker Kit (Sigma-Aldrich) and mixed by pipetting ...
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bioRxiv - Cell Biology 2020Quote: ... Osteogenic induction was performed in low-glucose DMEM medium supplemented with 50 μg mL−1 L-ascorbic acid 2-phosphate (Sigma-Aldrich), 10 mM glycerol 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... diluted in PBS for 2 h at room temperature and counterstained with 1:5000 Hoestch solution (bisBenzimide H 33258, Sigma-Aldrich). Sections were washed for 20 min in PBS (4 x 5 min rinses) ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Neuroscience 2020Quote: ... were homogenized with 1 ml H20 and extracted by vortexing with 2 ml of chloroform/methanol 2:1 (v/v) and 10 μl of synthetic internal standard (DAG 15:0/15:0, Sigma Aldrich), sonicated for 30 s ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed with 0.2% BSA three times for 5 min each and incubated with 1 μg/ml DAPI (Bisbenzimide Hoechst 33342 trihydrochloride, Sigma B2261) and secondary antibodies diluted 1:500 in 0.2% BSA for 1 h (Invitrogen donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The remaining lysate was then incubated with 25 μL (1 μL/2 OD600 nm of cells) of monoclonal anti-FLAG M2 (Sigma #F1804) at 4°C with rotation for 30 min ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... Fibroblasts were cultured in DMEM or tumour cell conditioned media supplemented with 2% FBS and 50 μg mL−1 ascorbic acid ascorbic acid (Sigma, A4403) on gelatin-coated plates ...
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bioRxiv - Biochemistry 2021Quote: ... chromatin isolated from 1–2 × 106 cells were incubated with 5 µg of mouse anti-FLAG (F1804, SIGMA-ALDRICH - RRID: AB262044) antibody pre-coupled to Protein A/G Sepharose ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (bovine serum albumin; Sigma Aldrich, Tween20, PBS) and subsequently incubated in primary antibody (cytokeratin 18 ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were blocked for 1 hour at room temperature in a 2% Abdil solution (bovine serum albumin; Sigma Aldrich, Tween20, PBS) and subsequently incubated in primary antibody (cytokeratin 18 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed 1-2 days later and the lysates were immunoprecipitated using anti-FLAG M2 affinity resins (10 μl per plate, Sigma Inc.). TNKS antibodies were as described (No ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with 5% skimmed milk for 1 hour at room temperature and then with primary antibodies diluted in 2% bovine serum albumin (Sigma-Aldrich) in tris-buffered saline 0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: HAE mucus was allowed to accumulate for one week prior to the application of 5 μl of 2 μm red-fluorescent (Cy3) polystyrene microspheres (1:50 dilution, Sigma-Aldrich) to the apical chamber of the transwell ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight with primary antibody against tyrosine hydroxylase diluted in 2% NDS in PBS/TX (1:1000 - rabbit anti-TH, Millipore AB152). The sections were rinsed with PBS (3×10min ...
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bioRxiv - Neuroscience 2020Quote: ... Axonal ChR2 expression was revealed by incubating slices for 1-2 h in Triton PBS containing 10% v/v NDS (D9663, Sigma, RRID:AB_2810235), then overnight at room temperature in Triton PBS containing 1% v/v NDS and Alexa Fluor 488-conjugated rabbit anti-GFP (1:500 ...
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bioRxiv - Physiology 2020Quote: ... Frozen tissue was sectioned into 2 mm coronal sections and a 1 mm diameter biopsy tool (Harris Micro-punch, Millipore-Sigma, Z708658) was used to collect tissue punches in ipsilateral regions distal and proximal to the infarcted tissue similar to IHC regions (Figure 3B) ...
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bioRxiv - Microbiology 2021Quote: ... Two-fold serial dilutions of an initial concentration of 5 μg/ml of the mAbs were incubated with NDV LaSota or rNDV-H5 for 3h at 37°C in complete medium supplemented with 50 ng/ml L-1-tosylamido-2-phenylethylchloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... for 45 minutes followed by 2 washes in a PBS buffer containing 1% FBS and 0.5% Saponin (Sigma, St Louis, MO). Intracellular staining for anti-IFNγ (2µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Each strain was grown in glucose-yeast extract-peptone (GYEP) liquid medium (0.3% yeast extract and 1% peptone; Difco, Soria Melguizo) with 2% glucose (Sigma-Aldrich, Spain) for 24 h to 48 h at 30°C ...
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bioRxiv - Cancer Biology 2021Quote: ... specific siRNAs (si-HER3-1: 5’-GCUGAGAACCAAUACCAGA, si-HER3-2: 5’-CCAAGGGCCCAAUCUACAA) and a validated control siRNA were obtained from Sigma-Aldrich.
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bioRxiv - Cell Biology 2021Quote: ... subcellular fractionation was performed on the remaining cells by suspending them in 10 ml cold hypotonic buffer (10 mM Tris pH 7.5, 10 mM KCl, 1 mM MgCl2, 10 mM glycerol 2-phosphate (Sigma-Aldrich #G9422), 1 mM EDTA ...
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bioRxiv - Developmental Biology 2022Quote: ... if fixed in 95% MeOH/5% GAA washes were done with PBDT (1XPBS/1%BSA/2%DMSO [dimethyl sulfoxide, Sigma Aldrich D8418]/0.5%Tween ...
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bioRxiv - Bioengineering 2022Quote: ... The residual proteins bound to exDNA were digested by 2-h incubation with 0.85 g L−1 proteinase K (Sigma-Aldrich, UK) at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... residual bound Coa and Efb were detected with anti-tag HRP-conjugated antibodies diluted 1:10000 in 2%BSA-PBST: α-HIS-tag antibody (A7058, Sigma) for EfbN ...
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bioRxiv - Cell Biology 2022Quote: ... [Ca2+]i responses to 80 mM KCl and 2) immunofluorescence in cells stained with smooth muscle-specific α-actin (SMA; 1:400, A2547, Sigma), calponin (1:100 ...
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bioRxiv - Synthetic Biology 2022Quote: ... HeLa cells subjected to Dox-induced expression of the PAR-T sensor proteins were treated with 20 μM PJ34 or 20 μM PDD 00017273 for 2 hours prior to induction of DNA damage by treatment with H2O2 (1 mM; Sigma, 216763) for 10 minutes ...
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bioRxiv - Pathology 2022Quote: ... 1 part NTA), incubated (30min., RT) and centrifuged (3000g, 1h, 4°C) in an Ultra-2 Centrifugal Filter Unite (Millipore, #UFC203024). Filtered samples (100µL ...
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bioRxiv - Microbiology 2022Quote: ... difficile strains were routinely grown in BHIS broth or on BHIS agar supplemented with 2-5 μg ml-1 thiamphenicol (Sigma-Aldrich) as needed [58] ...
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bioRxiv - Developmental Biology 2022Quote: ... (1 mg/ml) and incubated in 50 μl droplets of 10 µM 2′,7′- dichlorodihydro-fluorescein diacetate (H2DCFDA, D6883, Sigma-Aldrich) in PVA-PBS for 15 min at 37 °C in an atmosphere of 5% CO2 ...