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Citations for Millipore Sigma :
9151 - 9200 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and CH-5high/CH-6high subpopulation cancer cells (in the indicated cell numbers) were mixed with 100μl Matrigel/cell culture media mixture (Matrigel: cell culture media = 1:2) (Sigma or Corning). Indicated cancer cells/Matrigel/ cell culture media mixtures were injected into NOD/SCID female (for breast cancer xenograft ...
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bioRxiv - Biophysics 2022Quote: ... The tyrosinated tubulin level was detected by western blot using monoclonal anti-tyrosinated tubulin antibody (clone YL1/2; 1:2500 dilution; EMD Millipore) as primary antibody and a goat anti-rat secondary antibody (alkaline phosphatase-conjugated ...
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bioRxiv - Genetics 2022Quote: ... The pellet was resuspended in 1x PBS and cells were stained with a solution containing 1 µg/ml 4,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich India) for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... samples were transferred to BABB solution (1:2 ratio of benzyl alcohol, Sigma- Aldrich, 305197; and benzyl benzoate, Sigma-Aldrich, B6630) for at least 3 hr ...
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bioRxiv - Immunology 2021Quote: The right caudal lope of the lung was removed from the body and transferred on ice in PBS containing 1% BSA (w/v) and 2 mg/ml Actinomycin D (Sigma-Aldrich) for further processing ...
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bioRxiv - Microbiology 2021Quote: Freshly purified sporozoites (~10-50 million) were suspended in 1 ml of primary fix (2% freshly-prepared formaldehyde solution (Sigma-Aldrich), 2.5% electron microscopy grade glutaraldehyde (TAAB ...
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bioRxiv - Neuroscience 2021Quote: ... Three rats were subcutaneously injected with normal saline (Day 1, NaCl, 0.9% 10 mg/Kg) and D-SER (Day 2, 10 mg/Kg, Sigma-Aldrich Ireland) and placed in a box (24h x 38w x 38l cm) ...
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bioRxiv - Plant Biology 2021Quote: ... Then the seedlings were transferred into 1/2 MS liquid medium contained 50 μM CHX and 50 μM BFA (Sigma-Aldrich) for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed 2 times with 1X PBS and 1 time with MilliQ before mounting on microscope slides using Mowiol (10% Mowiol (Millipore, 475904), 0.1% diazabicyclo(2,2,2)-octane ...
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bioRxiv - Physiology 2020Quote: ... we also conducted 2 enzymatic lysis steps consisting of lysis solution 1 (50 mg/mL lysozyme and 20% RNase – Sigma R6148) at 37°C for 1 hour and lysis solution 2 (25 µL of 25% SDS ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
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bioRxiv - Immunology 2021Quote: ... and the cells were washed with PBS before the addition of 2% w/v agar containing 1 μg/ml-5 tosyl phenylalanyl chloromethyl ketone-trypsin (Sigma-Aldrich) to the cell surface ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 h at room temperature and finally counterstained for 1 min with Hoechst 33258 (10 μg/mL in PBS, Sigma-Aldrich). Images were taken using a confocal microscope LSM-800 Zeiss (28148) ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with protease and phosphatase inhibitors (1 tablet per 5ml buffer of cOmplete™, mini, EDTA-free protease inhibitor cocktail [Roche], 1:100 phosphatase inhibitor cocktail 2 [Sigma] ...
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bioRxiv - Molecular Biology 2022Quote: ... blocked in Pure Odyssey Blocking Buffer (Li-COR Biosciences, Lincoln) for 2 hours and incubated with an anti-ABCA4 clone 5B4 (1:1000; Sigma-Aldricht) and anti-Vinculin (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 2 h with the proper secondary antibodies (1:300, goat anti-rabbit Alexa-Fluor® 647, AP187SA6, Merck Millipore; 1:300 ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral plasmids containing shRNAs targeting STING (shSTING-1 #TRCN0000161345, shSTING-2 #TRCN0000163029) or non-targeted (scrambled) shRNA (shNT #SHC016-1EA) were purchased from Sigma Aldrich. STING-IRES-GFP and DN-IRES-GFP lentiviral plasmids were generated by inserting the cDNA of STING or STING-DN ...
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bioRxiv - Paleontology 2020Quote: All aqueous solutions were prepared from ultrapure grade water obtained by water filtration with a two stages Millipore system (Milli-Q® Academic with a cartouches Q-Gard 1 and Progard 2, Merck Millipore, Burlington ...
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bioRxiv - Synthetic Biology 2020Quote: ... The loading solution consisted of 15 µL of the protein sample mixed with 1 µL DTT and 15 µL Laemmli 2× Concentrate Loading Buffer (Sigma-Aldrich), and denatured at 95 °C for 10 min ...
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bioRxiv - Microbiology 2021Quote: TREx-BCBL-1-RTA cells were seeded onto poly-L-lysine coated coverslips and 2 µg/mL doxycycline hyclate (Sigma-Aldrich) was added 3 hours later ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Genetics 2020Quote: ... 50 mL of blocking buffer was prepared per 2×108 cells by adding 1 gram of biotin-free BSA (Sigma Aldrich) and 200 μl of 0.5 M pH 8.0 EDTA (ThemoFisher 15575020 ...
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bioRxiv - Cell Biology 2021Quote: ... we made 200 μl of gelation solution by mixing 188 μl of monomer stock solution (1 × PBS, 2 M NaCl, 8.625% (w/w) sodium acrylate (SA)(Sigma Aldrich 408220), 2.5% (w/w ...
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bioRxiv - Physiology 2021Quote: ... Then centrifuged for 5 minutes at 800 g at 5°C and the pellet was resuspended in 500 μl sorting buffer (1 % BSA wt/vol, 2 % skimmed milk powder (Sigma Aldrich), 1 mM EDTA in PBS pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... plus 0.1 % Triton X-100 and incubated overnight at 4 °C or for 2 h at room temperature with the following primary antibodies: anti-REST (1:500; 07-579 Millipore, MA), anti-Npas4 (1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... The samples were cut into small pieces and digested in 2 ml of digestion mix: 2mg/ml collagenase type 1 (#SCR103; EMD Millipore) in DMEM-F12 supplemented with 5% FBS (#10438026 ...
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bioRxiv - Immunology 2021Quote: ... the cells were fixed with 4% formaldehyde solution diluted in PBS during 2 hours and stained with 1% Naphtol blue black (Sigma, USA) solution for 1 hour for plaque visualization ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed twice in 0.1M PBS and blocked for 2 hours with 0.1M PBS containing 1% bovine serum albumin and Triton X-100 (0.1%) (both from Sigma Aldrich, Gilligham, UK) and then incubated for 48 hours at 4°C with the primary antibody ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted with 1 volume of 25:24:1 phenol-chloroform-isoamyl alcohol and precipitated in 2.5 volumes of 100% ethanol with 2 μL glycogen (Sigma Aldrich 10901393001). Pellet was washed once in 1 mL 100% ethanol and dissolved in 40 μL 10 mM Tris-HCl pH 8.5 ...
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bioRxiv - Plant Biology 2022Quote: ... Seeds were sterilized with (0.05% (v/v) Tween-20 solution for 10 minutes) and subsequently sown on 1/2 Murashige and Skoog (MS) medium (Sigma M5519) at 22□ in long-day conditions (16 h light/8 h dark) ...
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bioRxiv - Neuroscience 2021Quote: ... or if the number of ORs identified as responsive were more than five standard deviations away from the mean: 1% 2’-hydroxyacetophenone (Sigma H18607).
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bioRxiv - Developmental Biology 2021Quote: ... DiD staining solution was freshly prepared prior to every assay as a 1:200 dilution of the DiD stock solution [2 mg/mL dilution in N,N-dimethyl formamide (Sigma, #D4551)] in M9 1X buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... a control where females were fed a mixture of honey and water (1:2 w/w) or a treatment group fed a mixture of honey and deuterated water (Sigma Aldrich)(1:2 w/w ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... Acute deletion of floxed A20 and Abin-1 exons in vivo by oral gavage was performed using tamoxifen (2 mg/day, Sigma, T5648) for 3 consecutive days as indicated for survival analysis ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked with 2% donkey serum and then immunostained using rabbit antibody against Cx43 (Figure 6A, Sigma, cat# C6219, 1:500) or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2 ...
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bioRxiv - Immunology 2020Quote: ... and the cells were washed with PBS before the addition of 2% w/v agar containing 1 μg/mL-5 tosyl phenylalanyl chloromethyl ketone-trypsin (Sigma-Aldrich,) to the cell surface ...
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bioRxiv - Developmental Biology 2020Quote: ... They were then rinsed 4 times with the same medium free of GFP-nanobodies and fixed at room temperature for 2 hours with PBS supplemented with 4% paraformaldehyde (PFA) and 1% BSA (A7638 Sigma-Aldrich). Open-books were observed with a STED microscope (TCS SP8 ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were blocked in %5 BSA for 2 h at room temperature and incubated with anti-desmin antibody (1:20; Sigma, D8281) for two overnights ...
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bioRxiv - Immunology 2021Quote: ... The only two variations from the written protocol were (1) an open wash dispense after the peroxide step and (2) DAPI (Sigma D9542) was dispensed twice at the end of the protocol at a concentration of 1 µg/mL ...
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bioRxiv - Genomics 2021Quote: ... the supernatant was removed by aspiration and the nuclear pellet was resuspended in 200 uL staining mix (2% normal goat serum, 0.1% BSA, 1:500 anti-NeuN conjugated to AlexaFluor488 (Millipore, cat#: MAB377X) in PBS ...
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bioRxiv - Cell Biology 2021Quote: Mission plasmids directing expression of shRNAs targeting PlexinA2 (TRCN0000061499 (ShPlexA2#1) and TRCN0000061501 (ShPlexA2#2)) or PlexinA4 (TRCN0000078683) were purchased from Sigma Aldrich. The production of the lentiviruses ...
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bioRxiv - Immunology 2020Quote: ... The swabs were placed into 1 ml of Trizol or 2 ml of virus transport medium comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... the respective drugs were serially diluted (1 in 2) in liquid YPD with the highest concentrations for myriocin (Sigma Aldrich, M1177) at 128 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... albicans yeast at the ratio of 1:20 in RPMI 1640 medium containing 2% heat-inactivated mouse serum (#S3509, Sigma-Aldrich) at 37°C for 3 h.
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bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
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bioRxiv - Biophysics 2023Quote: ... bacteria were grown in isotope-enriched M9 minimal media containing 1 g/l of 15N-ammonium chloride and 2 g/l of 13C-glucose (Sigma, USA) supplemented with 1.7 g/l YNB (Yeast Nitrogen Base ...
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bioRxiv - Cell Biology 2023Quote: ... The washed nuclei pellets were resuspended in sorting buffer (2% BSA/RNase-free PBS) and labeled with anti-PCM1 (1:500, HPA023374, Sigma-Aldrich) on ice for 45 minutes ...
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bioRxiv - Microbiology 2023Quote: Synechococcus WH7803 wild type and mutant cultures were grown in constant light at 12 μmol quanta m−2 s−1 in Pro99 media amended with high molecular weight chitosan or colloidal chitin (Millipore Sigma) to a final concentration of 56 μg/ml in triplicates ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated (2 h, room temperature) with the relevant secondary antibody (see above) and 5 min with DAPI (1:1000, Sigma D817) as a nuclear counterstain ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2.5 h at 37°C and 5% CO2 in 2% FBS DMEM supplemented with 100 µg ml-1 cycloheximide (#C4859, Sigma-Aldrich) to prevent viral proteins synthesis ...