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Citations for Millipore Sigma :
9001 - 9050 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... After 4 days plants were transferred to agar plates with or without 65 µM of SHAM (Salicylhydroxamic acid; Sigma Aldrich, USA), then grown 3 days at 22°C followed by 3 days at 10°C or 6 days at 22°C (control) ...
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bioRxiv - Plant Biology 2020Quote: ... with complete immersion of the caryopsis in a 50% sulphuric acid solution (ACS reagent grade H2SO4, Sigma-Aldrich, St Louis, USA) for an optimal interval allowing for the weakening of floret structures without reducing germination potential ...
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bioRxiv - Cell Biology 2021Quote: ... DLD1-wildtype and DLD1-NXF1-AID cells were pretreated for 0-8 hrs with 0.5 mM synthetic auxin (3-indoleacetic acid, Sigma, in ethanol), prior to addition of 250 nM NVP-2 ...
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bioRxiv - Plant Biology 2021Quote: ... Three seedlings per treatment were sampled and 300 mg fresh weight of the plant material was placed on a Petri dish filled with 2 mL of 1X Phosphate-Buffered Saline (PBS) + 20 mM Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich). The tissue was finely chopped with clean razor blades to release nuclei ...
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bioRxiv - Plant Biology 2021Quote: ... (6.7 g/L yeast nitrogen base without amino acids, 20 g/L glucose, appropriate concentration of relevant Yeast Synthetic Drop-out Medium; Sigma-Aldrich) and incubated three days at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... of a nucleic acid extract of each water type as well as into reagent-grade water (Milli-Q, Water Purification System, Millipore, Ontario) to serve as a positive control and subject to PCR as described above ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA reaction was diluted 1:10 in water and used for qPCR analysis with primers directed against BR marker genes EXPA8 and DWF4 or against RLP44.The SYBRR Green I nucleic acid gel stain (Sigma-Aldrich) was used for detection ...
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bioRxiv - Neuroscience 2021Quote: ... PBA solution was prepared by dissolving PBA in 0.9% normal saline and then titrating equimolecular amounts of 4-phenylbutyric acid (Sigma, Madrid, Spain) and sodium hydroxide to pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant His-tagged proteins were purified from cell lysates on a nickel-chelating column (Ni-nitrilotriacetic acid agarose; His-select high flow nickel affinity gel; Sigma-Aldrich), as described previously [53] ...
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bioRxiv - Cell Biology 2021Quote: ... Cellular contractility was modulated by incubating tissues with Oleoyl-L-α-lysophosphatidic acid (LPA) at a final concentration of 20µM (L7260, Sigma-Aldrich), or (−)-Blebbistatin at a final concentration of 50µM (B0560 ...
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bioRxiv - Cell Biology 2021Quote: Labeling reagents XTT (sodium 3’-[1-(phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) and PMS (N-methyl dibenzopyrazine methyl sulfate) were purchased from Sigma-Aldrich. Assay was performed according to protocol in Cell proliferation kit II (XTT ...
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bioRxiv - Biochemistry 2022Quote: ... Buffers were made by adjusting Milli-Q water using 5 M NaOH and 80% L-(+)-Lactic acid (27715, Sigma-Aldrich, Germany). Gelatin was prepared by hydrating porcine gelatin (G1890 ...
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bioRxiv - Cell Biology 2022Quote: ... Antibiotics (penicillin and streptomycin) and fatty acid (FA)-free bovine serum albumin (BSA) were purchased from Sigma-Aldrich (St Louis, MO). FBS (Optima ...
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bioRxiv - Systems Biology 2022Quote: ... 0.5% formic acid binding solution and were loaded on 10 μL of phosphopeptide enrichment IMAC resin (PHOS-Select™ Iron Affinity Gel, Sigma) already washed and conditioned with binding solution in custom made filter tips fitted on Eppendorf tubes caps ...
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bioRxiv - Biochemistry 2022Quote: ... Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629) ...
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bioRxiv - Biochemistry 2022Quote: ... Dimethyl LV labeling for geminal pair determination used 150 mg/L 2-Keto-(3-methyl-13C)-butyric-4-13C,3-d acid sodium salt (Sigma 589063). Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887 ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Molecular Biology 2022Quote: ... acetyl-enriched peptides were eluted from the antibody-bead conjugates with 0.1% trifluoroacetic acid in water and were desalted using C-18 zip-tips (Millipore, Billerica, MA). Samples were vacuum dried and re-suspended in 0.2% FA in water.
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bioRxiv - Bioengineering 2022Quote: Films (N = 5 per condition) were lyophilized and incubated for 48 h in 5 wt% trichloroacetic acid (TCA, T6399, Sigma-Aldrich). Scaffolds (N = 5 per condition ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Osteoclast resorbed surface (Oc.S/BS) was measured following TRAP staining with Leukocyte Acid Phosphatase TRAP Kit (Sigma Aldrich, catalog # 387A-1KT). RNA was extracted from isolated whole femur for qPCR for osteoblast (Col1a1 ...
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bioRxiv - Neuroscience 2022Quote: ... Fixed samples were washed in distilled water for 30 minutes before 21 days of decalcification in 14% (v/v) ethylenediaminetetraacetic acid (EDTA, Sigma, UK) solution (pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Flash frozen pellets were resuspended in 700 µL Trizol with 350 µL acid washed and autoclaved glass beads (425-600 µm, Sigma G8772). The cells were disrupted using a vortex on top speed for 7 cycles of 15 seconds (the samples were chilled on ice for 30 seconds between cycles) ...
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bioRxiv - Bioengineering 2022Quote: ... The two-post array supporting the newly formed hydrogels was then lifted out of the casting plate and transferred to a fresh 24-well plate containing Lonza growth medium supplemented with 2 g/L aminocaproic acid (Sigma-Aldrich) to inhibit fibrinolysis ...
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bioRxiv - Biochemistry 2022Quote: ... Control cultures were swapped from SM supplemented with 13C-glucose to SM solely supplemented with 12C-amino acids (12C-AA; Sigma #Y1896) at standard culturing concentrations.
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bioRxiv - Cancer Biology 2022Quote: ... with 1X protease and phosphatase inhibitor cocktail (Fisher, USA), and 1X DTT (Fisher, USA) and was quantified via a bicinchoninic acid assay (Sigma, USA). The lysates were then separated using SDS–PAGE and electroblotted onto polyvinylidene difluoride membranes ...
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bioRxiv - Biochemistry 2020Quote: ... The precipitated proteins were used for determination of protein content using a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich, Taufkirchen, Germany).
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bioRxiv - Microbiology 2021Quote: ... Total carbohydrate was determined by the Dubois phenol-sulfuric acid method (76) adapted to a 96-well plate assay with the Total Carbohydrate Assay Kit (MAK 104, Sigma-Aldrich), using D-glucose as the standard and absorbance read at a wavelength of 490 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... and were cultured in 0.5X MBS + 0.2% fatty acid free BSA (Fisher BP9704) + 50 ug/mL gent with the following concentrations of factors: 1uM dexamethasone (DEX; Sigma D4902); 1uM cycloheximide (CHX ...
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bioRxiv - Molecular Biology 2020Quote: ... Neurons were counted and plated at a density of 80,000 cells/cm2 in the BME-based buffer on coverslips that had been etched with 70 percent nitric acid (Sigma-Aldrich) before being coated with 1 mg/mL poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... the ERS alleviator 4-phenyl butyric acid (4-PBA, 5 mM) and the ATF6 inhibitor Ceapin-A7 (500 nM) were purchased from Sigma-Aldrich. Chondrocytes were cultured in DMEM supplemented with Tm or Tg for 6 h ...
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bioRxiv - Cell Biology 2021Quote: ... Haploid cells were selected in SD-Leu (for Mat alpha selection) and -Arg-Lys with toxic amino-acid derivatives canavanine and thialysine (Sigma-Aldrich) to select against diploids ...
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bioRxiv - Microbiology 2020Quote: Protein concentration was determined by the bicinchoninic acid protein assay kit (BCA-Pierce, USA) using bovine serum albumin (BSA, Sigma-Aldrich) as standard.
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bioRxiv - Microbiology 2020Quote: ... CD14+ macrophages differentiated for 7 days in M-CSF (described above) were detached from the petri dish using 2 mM ethylenediaminetetraacetic acid (EDTA, Sigma Aldrich) in PBS at 4 °C as well as mechanical scraping ...
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bioRxiv - Cell Biology 2020Quote: Fully differentiated C2C12 MS2-CAS myotubes were starved in serum-free DMEM for 2 hrs before a 3-hr incubation in glucose-free or low-glucose DMEM/fatty acid-free BSA (Sigma-Aldrich) 0.2% supplemented with a mixture of cold and radiolabeled palmitate (Palmitic Acid ...
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bioRxiv - Physiology 2020Quote: ... 5 μm tissue sections were subject to antigen retrieval (0.01 M citric acid and 0.1% Triton X) (Sigma, St. Louis, MO), blocked with the avidin/biotin blocking kit (Vector Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... Mass spectrometry analysis were performed on a Bruker autoflex® speed TOF/TOF mass spectrometer in negative reflective mode with delayed extraction using as matrix an equal volume of dihydroxybenzoic acid matrix (Sigma-Aldrich) dissolved in (1:2 ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were then centrifuged at 2,500 × g for 10 min at 4°C and proteins from the supernatant were precipitated using 10% tricholoroacetic acid (TCA) with acetone (Sigma-Aldrich). 1,700 μl of TCA-acetone was added to 300 μl of culture supernatant and incubated at −20°C for one hour ...
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bioRxiv - Biochemistry 2021Quote: The competitive inhibition of human fumarase activity in the presence of 2-amino-3-phosphonopropionic acid (AP-3, Sigma-Aldrich A4910) was fluorometrically assessed using a coupled enzyme assay ...
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bioRxiv - Neuroscience 2021Quote: ... administration of apomorphine hydrochloride (0.25 mg/kg) dissolved in physiological saline containing 0.1% ascorbic acid (Sigma Aldrich, St. Louis, Missouri, USA). Two priming injections of apomorphine (1-week interval off-drug ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1 μM LDN193189 (Stemgent, #04-0074), 3μM CHIR-99021 (Selleck, #51263) and 10 μM Ascorbic Acid (AA) (Sigma, #A 0278-100G).The “N2B27” medium is composed of equal volumes of ADMEM/F12 (Thermofisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... The following nucleic acids were used: a pool of RNA consisting of 5000-8000 Da from Torula yeast (Sigma-Aldrich, R6625), ssTRS(+) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Transformants were plated on SDCAA-agar (1.71 g/L YNB without amino acids and ammonium sulfate [Sigma-Aldrich #Y1251], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We recovered transformants in a molten SDCAA agarose gel (1.71 g/L YNB without amino acids and ammonium sulfate (Sigma-Aldrich #Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... This M0 medium was used as a basis for the development of media in which the only carbon source was sodium acetate (60 mM) or oleic acid (Sigma-Aldrich) (6 mM ...
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bioRxiv - Bioengineering 2022Quote: ... the 11-mercaptoundecanoic acid (CAS no: 71310-21-9) and L-Tryptophan (CAS no: 73-22-3) were obtained from Sigma Aldrich. The RBD ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated for 48 h in the presence of the ncAA BCN-Lysine (0.5 mM; Synaffix, Oss, Netherlands) in growth media supplemented with 100 μM ascorbic acid (Sigma Aldrich, Israel).
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Interactome comparison of human embryonic stem cell lines with the inner cell mass and trophectodermbioRxiv - Developmental Biology 2019Quote: ... At embryonic day 6 the zona pellucida of the embryos were removed by brief treatment with Acid Tyrode’s solution pH 5.0 (Sigma-Aldrich, Gillingham, UK), and denuded blastocysts were washed in ISM2 (Medicult) ...
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bioRxiv - Synthetic Biology 2020Quote: ... magnesium sulfate (MgSO4), calcium chloride (CaCl2), 3-(N-morpholino)propane sulfonic acid (MOPS), and hydrogen peroxide (H2O2, 30%) were from Sigma Aldrich. N-3-oxo-dodecanoyl-L-Homoserine lactone (AHL ...
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bioRxiv - Genomics 2019Quote: ... On day 0 media was changed to RBA media (RPMI 1640 with glutamine [ThermoFisher] supplemented with 500 μg/ml BSA and 213 μg/ml ascorbic acid [both from Sigma-Aldrich]) supplemented with 4 μM CHIR-99021 ...
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bioRxiv - Genomics 2019Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B-27 with penicillin/streptomycin, and 5 mg/mL aminocaproic acid, Sigma-Aldrich). 3D-EHTs were supplied with 2.5 mL/well of fresh 3D-EHT media three times per week.