Labshake search
Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
-
bioRxiv - Plant Biology 2021Quote: ... MALDI-ToF-MS was performed from gel extract solutions which were pipetted on an α-Cyano-4-hydroxycinnamic acid (≥98%, Sigma) prepared on a stainless-steel plate (0.5 μL of a 10 mg/mL ACN/H2O 1:1 solution) ...
-
bioRxiv - Cancer Biology 2021Quote: ... for 15 min before addition of 5 mM ethylendiaminetetraacetic acid (EDTA) and washed with PBS before resuspended in CRC organoid medium with 10 µM Y-27632 (Sigma-Aldrich). Organoids were stimulated with 200 ng/mL IFNγ (Peprotech ...
-
bioRxiv - Microbiology 2020Quote: ... were infected with cell-free VZV pOka (20 µL of 4 x 104 PFU/mL) in the presence or absence of phosphonophormic acid (200 µg/mL) (Sigma-Aldrich) for 1 hr ...
-
bioRxiv - Cell Biology 2019Quote: ... Supernatant was either directly added to an equal volume of 2X Laemmli buffer containing 2% β-mercaptoethanol or precipitated with trichloroacetic acid (Sigma) and washed with acetone prior to resuspension in 2X Laemmli containing 2% of β-mercaptoethanol ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins in the media fraction were precipitated by adding 0.15 ml of 100% trichloroacetic acid (TCA) (Sigma Chemical, St Louis, MO) and incubated on ice for 30 min ...
-
bioRxiv - Cell Biology 2020Quote: ... 50 μg/mL ascorbic acid in H2O (Sigma, cat. no. A8960-25G, 4.5×10-4M MTG (Sigma, cat. no. M1753-100ML)) with 500 nM SB431542 (Selleckchem ...
-
bioRxiv - Cell Biology 2020Quote: ... and 0.5% deoxycholic acid) containing a protease and phosphatase inhibitor cocktail (cOmplete™ Protease Inhibitor Cocktail and PhosSTOP™ from Sigma). Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to an Immobilon polyvinylidene fluoride membrane (EMD Millipore ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were grown for 3 days on coverslips until they reached confluence and then 50 ug/ml L-ascorbic acid-2-phosphate (Sigma Aldrich) was added to cultures ...
-
bioRxiv - Cell Biology 2020Quote: ... SILAC-labelled samples were mixed with equal amount of the input sample and they were diluted with equal volume of 0.1% Trifluoroacetic acid (TFA) (Sigma Aldrich, UK) and spun onto StageTips as described by Rappsilber et al 81 ...
-
bioRxiv - Cell Biology 2020Quote: Periodic acid-Schiff (PAS) staining was performed to stain mucin-producing goblet cells in murine EDMs using a PAS Kit (Sigma-Aldrich) as described previously [8] ...
-
bioRxiv - Microbiology 2021Quote: ... and 1x non-essential amino acid) and 50 ng/mL of human macrophage colony-stimulating factor (M-CSF, Millipore, Temecula, CA) for 5-6 days at 37°C and 5% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... Mass spectrometry analyses were performed on a Bruker autoflex™ speed TOF/TOF mass spectrometer in negative reflective mode with delayed extraction using as matrix an equal volume of dihydroxybenzoic acid matrix (Sigma-Aldrich) dissolved in (1:2 ...
-
bioRxiv - Developmental Biology 2020Quote: ... a complementary DNA (cDNA) fragment encoding amino acids 448 to 622 of mouse Zcwpw1 was inserted into the p-ET-32a + vector (EMD Millipore) and transfected into BL21-CodonPlus (DE3 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and the suspension was transferred into a 2 mL tube containing 50 mg of 425 – 600 μm acid-washed glass beads (Sigma-Aldrich). The bacterial cells were disrupted using a Fast prep FP120 homogenizer (Thermo Savant Bio101 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... following up with a screen in SD-Trp (1.71 g/L Yeast Nitrogen Base Without Amino Acids and Ammonium Sulphate (Sigma-Aldrich, Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2021Quote: SH-SY5Y cells at 60 – 70% confluency were induced to differentiate using 13-cis retinoic acid at a concentration of 2 µM (Sigma-Aldrich) in the growth media ...
-
bioRxiv - Neuroscience 2020Quote: ... Neuronal cultures were maintained in neuronal medium (Neurobasal/B27 medium supplemented with 20 ng/ml BDNF, 10 ng/ml GDNF, both from PreproTech, and 20 ng/ml L-ascorbic acid, Sigma Aldrich).
-
bioRxiv - Cell Biology 2022Quote: Affinity-purified rabbit anti-AQP5 IgG antibody targeted towards amino acid residues 249-265 of rat AQP5 (AB15858) and normal goat IgG antibody (NI02-100UG) were obtained from Millipore Sigma. Normal rabbit IgG antibodies were obtained from Cell Signaling Technology (2729 ...
-
bioRxiv - Microbiology 2019Quote: ... agar supplemented with 200 ppm and 512 ppm of nalidixic acid (nal) and rifampicin (rif) (Sigma-Aldrich Corp, St. Louis, MO), respectively ...
-
bioRxiv - Genetics 2019Quote: ... pellets were resuspended in 400 μl cold LB and mixed with the same volume of acid-washed glass beads (Sigma Aldrich). The mixture was then transferred to a FastPrep-24TM 5G Instrument (MP Biomedicals ...
-
bioRxiv - Biophysics 2019Quote: ... cover glasses cleaned with ethanol were incubated for 30 minutes in 10 mg/ml BSA solution (fatty acid free, A1595 Sigma-Aldrich) and rinsed with double distilled water before usage.
-
bioRxiv - Microbiology 2019Quote: ... Embryos were maintained at 28.5°C in egg water containing 60 µg / ml Tropic Marin Sea Salt (Norwood Aquarium) and anaesthetised with egg water containing 200 µg / ml buffered tricaine (3-aminobenzoic acid ethyl ester) (Sigma-Aldrich) during bacterial injections and imaging ...
-
bioRxiv - Neuroscience 2019Quote: ... cells at 14 DIV were treated with culture medium containing 100 µg/ml L-ascorbic acid 2-phosphate (vitamin C, Sigma, 49752) for 24 h ...
-
bioRxiv - Cell Biology 2019Quote: ... L-lysine-13C6, 15N2 (Lys-8, #608041)) and L-arginine-13C6, 15N4 (Arg-10, #608033) stable isotope labelled amino acids (Sigma-Aldrich). Media was also supplemented with 10% dialysed FCS (Sigma) ...
-
bioRxiv - Cell Biology 2019Quote: ... The flow-through containing released sialic acid was dried and derivatized using 1,2-diamino-4,5-methylene-dioxybenzene (DMB) (Sigma-Aldrich, D4784-10MG), as previously described (96) ...
-
bioRxiv - Genetics 2020Quote: ... Single-culture and competitive outgrowth kinetics were done in low fluorescence YNB medium (YNB-lf) with 2% glucose and complete amino-acids supplement mix (Sigma Y1501 completed with Sigma U0750)(Sheff and Thorn ...
-
bioRxiv - Cell Biology 2019Quote: ... Gels were then fixed for 30 min at RT in fixing solution (40% [v/v] methanol, 2% [v/v] acetic acid) and stained overnight with Brilliant Blue G-Colloidal Concentrate (Sigma-Aldrich), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... larvae were kept under anesthesia in egg water containing 0.02% buffered 3-aminobenzoic acid ethyl ester (Tricaine, Sigma-Aldrich, the Netherlands). The culture of zebrafish with mutations in immune genes was approved by the local animal welfare committee (DEC ...
-
bioRxiv - Bioengineering 2019Quote: ... the presence of structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein and proteoglycans) was investigated with Periodic Acid Schiff staining (Sigma-Aldrich) as described in the protocol.
-
bioRxiv - Plant Biology 2020Quote: ... The absorbance of the reaction product was measured at 725 nm and the phenolic content was expressed as milligrams per gram of dried extract based on the calibration curve prepared for chlorogenic acid (Sigma-Aldrich). The results are given as means ± SD (n = 3).
-
bioRxiv - Molecular Biology 2020Quote: ... flash frozen pellets were resuspended in 700 µl Trizol with 350 µl acid washed and autoclaved glass beads (425-600 µm, Sigma G8772). The cells were disrupted using a vortex on top speed for 7 cycles of 15 seconds (the samples were chilled on ice for 30 seconds between cycles) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Each culture of 15 mL was divided into 2-mL tubes along with 100 μL of acid-washed glass beads (150-212 μm; G1145; Sigma-Aldrich) and 50 μL of lysis buffer supplemented with x1 Complete Mini EDTA-free (Roche ...
-
bioRxiv - Genetics 2021Quote: The CTCF protein was depleted in CTCF-AID mESCs by adding 500 μM of indole-3-acetic acid (IAA, a chemical analog of Auxin; Sigma-Aldrich) to the medium for 24 hrs ...
-
bioRxiv - Genomics 2021Quote: ... cutting through the hull and tip of cotyledons before soaking in a 100 PPM solution of gibberellic acid (Sigma-Aldrich, Inc.) for 60 minutes ...
-
bioRxiv - Plant Biology 2020Quote: ... with the following modification: guard cell lysis was performed by adding glass beads (425 μm - 600 μm diameter, acid washed, from Sigma-Aldrich) and 350 μl RULT buffer to the sample ...
-
bioRxiv - Neuroscience 2020Quote: ... Experiments were carried out in 8-12-week-old C57Bl/6 male mice bred at RCSI.26 SE was induced in fully awake mice via a microinjection of kainic acid (KA) (0.3 µg in 0.2 µl phosphate-buffered saline (PBS)) (Sigma-Aldrich, Dublin, Ireland) into the basolateral amygdala ...
-
bioRxiv - Biochemistry 2021Quote: ... of 5-hexynoic acid was added to a 50 mL round bottom flask followed by 15 mL DMF (anhydrous, Sigma Aldrich), 1.7 g (8.9 mmol ...
-
bioRxiv - Biochemistry 2021Quote: ... A 150-µl aliquot was mixed with an equal volume of Annexin V buffer containing 5 mg/ml fatty acid-free BSA (Sigma-Aldrich) and 5 nM SYTOX red (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: A reference standard of 38 fatty acid methyl ester standards was prepared by mixing a purchased 37 fatty acid methyl ester (FAME) standard (Restek, Bellefonte, PA, USA) and 450 μM methyl-nonadecanoate in MTBE (Sigma Aldrich) at a 1:9 ratio ...
-
bioRxiv - Bioengineering 2021Quote: ... 500 µL of the reaction mixture was taken from each tube and mixed with 500 µL of a solution of 0.25% thiobarbituric acid (TBA; Sigma, Cat.# T5500) in 15% trichloroacetic acid (TCA ...
-
bioRxiv - Bioengineering 2021Quote: A 1 w.t.% suspension of insoluble type I bovine dermal collagen (Devro) was prepared in 0.05 M acetic acid solution (Sigma-Aldrich UK). The mixture was left at 4°C to swell for 24 hours and homogenized in a blender at 22,000 rpms for 6 minutes ...
-
bioRxiv - Bioengineering 2021Quote: ... two additional rats were implanted with midline cannulae and T1-weighted image series were acquired during injection of gadolinium diethylenetriaminepentaacetic acid (Gd-DTPA, Sigma-Aldrich). Each animal was prepared for imaging as described above ...
-
bioRxiv - Bioengineering 2021Quote: ... the N2a cells were stimulated by a reduced serum medium (DMEM supplemented with 2% FBS) in the presence of 20 μM Retinoic acid (RA; Sigma-Aldrich). The medium was refreshed every 24 h ...
-
bioRxiv - Bioengineering 2020Quote: ... 15 μL droplets of cell-hydrogel mix were placed on the surface of elastomeric membranes that underwent a silanization process through a nitric acid and (3-Aminopropyl)triethoxysilane (Sigma-Aldrich) treatment ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 × 106 PFU of MNV-1.CW3 were diluted in PBS in the presence or absence of 1 μg of IgA MAb MAb 2D3 or 4F9 and in the presence or absence of 500 μM bile acid (sodium glycochenodeoxycholate GCDCA, or sodium taurocholate hydrate TCA, Sigma Aldrich) followed by enumeration by plaque assay ...
-
bioRxiv - Neuroscience 2021Quote: ... The sections selected for anti-Aβ were first treated with 88% formic acid (Sigma-Aldrich, No. 251364, St. Louis, MO, USA) to ensure specific plaque immunostaining ...
-
bioRxiv - Neuroscience 2021Quote: ... Neuronal cultures were maintained in neuronal medium (Neurobasal/ B27 medium supplemented with 20 ng/ml BDNF and 10 ng/ml GDNF, both from PreproTech; and 20 ng/ml L-ascorbic acid, Sigma-Aldrich). MFCs were made with Sylgard 184 silicone elastomer kit (Dow Corning ...
-
bioRxiv - Biophysics 2020Quote: ... a mixture of 100 μL of 40% acrylamide 50 μL of 2% Bis-acrylamide and 0.8 μL of Acylic-Acid (Sigma Aldrich, Germany) was generated (ABA-mix) ...
-
bioRxiv - Neuroscience 2020Quote: ... SH-SY5Y cells were differentiated to a neurone-like phenotype prior to experimentation by incubation with 10 μM trans-retinoic acid (Sigma, UK) for 5 days [26].