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Citations for Millipore Sigma :
9051 - 9100 of 10000+ citations for 6 fluoronaphthalene 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Cells were then incubated for 48 h in the presence or absence of 400 μM sodium oleate or linoleic acid sodium salt (≥98%, Sigma-Aldrich) in DMEM supplemented with 0.5% BSA and 1% penicillin-streptomycin ...
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bioRxiv - Genomics 2019Quote: ... of lysis buffer (RLT for RNeasy or TRI reagent for Direct-zol) and acid-washed glass beads (425-600 micron, Sigma-Aldrich). RNA was then purified according to each manufacturer’s protocol for yeast ...
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bioRxiv - Pathology 2020Quote: ... 3D-EHTs were transferred from the agarose molds to 24-well tissue culture dishes containing 3D-EHT media (RPMI-1640 media supplemented with B-27 and 5 mg/mL aminocaproic acid [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2020Quote: Cu(I) accumulation after the course of the RclA reaction was measured using bathocuproinedisulfonic acid disodium salt (BCS, Sigma Aldrich B1125). NADH oxidation reactions were carried out as in the previous section but were started with either NADH (200 µM final) ...
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bioRxiv - Molecular Biology 2020Quote: ... was separated from the bottom oil layer and transferred to a nucleic acid purification column (Corning Costar Spin-X column, 0.45 μm, Sigma-Aldrich, USA). Tubes were centrifuged for 1 minute at 16000 g at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The resultant pellets were resuspended in 100 μl of 1X PBS then incubated with 7 μl of 4% (w/v) phosphotungstic acid (Sigma-Aldrich) in 0.2 M magnesium chloride ...
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bioRxiv - Cell Biology 2021Quote: ... Tween-20 (P9416), ethylenediaminetetraacetic acid disodium salt dehydrate (EDTA, E4884), Trizma base (Tris, 93349) and ciprofloxacin (I7850) were purchased from Sigma-Aldrich. Complete protease inhibitor cocktail (04693116001 ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana or Arabidopsis leaf discs were floated on water supplemented with 25 µM flg22 (Hycultec) or 50 µM abscisic acid (ABA; Sigma-Aldrich) for 2 h as described in Lozano-Durán et al ...
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bioRxiv - Physiology 2020Quote: ... and seeded onto 6-well plates (105 cells/well) in osteogenic medium (50μg/mL L-ascorbic acid-2-phosphate and 5mM β-glycerophosphate) (Sigma, UK) for 24 days ...
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bioRxiv - Microbiology 2020Quote: ... The internal standard d10-Ritalinic acid (d10-RA) hydrochloride solution (100 μg/mL as a free base; Sigma-Aldrich, The Netherlands) was added to all samples at a final concentration of 2 ng/μL as an internal standard for accurate quantification ...
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bioRxiv - Microbiology 2020Quote: ... Macrophage-like THP1 cells were pre-incubated with L-glutamic acid (5mM) or Sodium Pyruvate solution (2mM, Sigma catalog# S8636-100ml) for 2 hours before stimulating with Escherichia coli serotype O127:B8 LPS (50ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... or the same isosmotic solution with 100 µM of the general anion channel inhibitor 5-Nitro-2-(3-phenylpropylamino) benzoic acid (NPPB, Sigma-Aldrich), which inhibits malarial new permeation pathways (59) ...
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bioRxiv - Neuroscience 2020Quote: ... beta-citronelol (cat# C83201), geraniol (cat#16333, 4-methylcatechole (cat# M34200) and arachidonic acid (cat#A3611) were all purchased from Sigma-Aldrich.
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bioRxiv - Plant Biology 2021Quote: ... the three terminal leaflets of three leaves from the middle third of ten plants were sprayed on the adaxial surface with 900 uL of a solution of JA (Jasmonic Acid, Liquid Bioreactive SIGMA -JA), each leaflet was sprayed with 100 uL at a concentration of 150 μg mL-1 plus tween 80 at 0.01% using an atomizer [31] ...
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bioRxiv - Microbiology 2019Quote: NA activities of recombinant AIV strains were measured using the fluorogenic substrate MUNANA (2’-[4-Methylumbelliferyl]-alpha-D-N-acetylneuraminic acid; Biosynth and Sigma-Aldrich), as described previously [70] ...
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bioRxiv - Immunology 2021Quote: ... The protein concentration in the final samples was measured with the use of Bicinchoninic Acid Kit for Protein Determination (Sigma-Aldrich) and a 96-well plate analyzer (Tecan Infinite 200 PRO).
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bioRxiv - Cell Biology 2019Quote: ... The serum-free medium –SerM was prepared as described previously by replacing 10% v/v heat-inactivated human serum with oleic and palmitic acid (Sigma Aldrich) in a final concentration of 30 µM each ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then resuspended in 10 ml of PBS supplemented with 2 mM DTSP (3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester dissolved in DMSO, a crosslinking agent from Sigma, MO, USA) and incubated for 30 min ...
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bioRxiv - Plant Biology 2020Quote: SA and SAG were quantified against an internal standard curve with ten calibrationpoints and two internal standards (p-nitrophenol, Fluka; camphorsulfonic acid, Sigma-Aldrich) at 1 mg l-1 ...
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bioRxiv - Cell Biology 2019Quote: ... followed by flipping into a fibronectin coated coverslip to passivate the new-top surface with a solution of 0.2% pluronic acid (Sigma, P2443-250G).
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bioRxiv - Neuroscience 2021Quote: ... Alcian Blue/periodic acid-Schiff (PAS) stain was conducted according to standard procedure with a Harris hematoxylin (Sigma-Aldrich, C.I.75290) counterstain ...
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bioRxiv - Genetics 2020Quote: ... From d14 to day 24 cells were kept in Glial Maturation Medium (GMM) in absence of growth factors and supplemented with 20 μg/ml of ascorbic acid (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... USA) was added and incubated for 10 min before the reaction was stopped with 100 μl 1M Phosphoric Acid (Sigma-Aldrich). The optical density was measured at 450 nm (OD450 nm ...
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Plasma derived cell-free mitochondrial DNA originates mainly from circulating cell-free mitochondriabioRxiv - Genomics 2021Quote: ... Anticoagulant Citrate Dextrose Solution A (ACD-A) buffer (0.1M trisodium citrate, 0.11M glucose and 0.08M citric acid) and Prostaglandin E1 (1μM) (Sigma-Aldrich, St. Quentin Fallavier Cedex) were then added to the plasma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Blood samples from vancomycin-treated animals were immediately transferred to a disodium ethylenediaminetetraacetic acid (EDTA, [Sigma-Aldrich Chemical Company, Milwaukee WI]) treated microcentrifuge tube and centrifuged at 3000 rpm for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... at which point the fibrin gels were sufficiently polymerized around the posts to be lubricated in media and transferred from the casting troughs into a 24-well plate with fresh 3D-EHT media (RPMI-B27 with penicillin/streptomycin, and 5 mg/ml aminocaproic acid; Sigma-Aldrich). 3D-EHTs were supplied with 2.5 ml/well of fresh 3D-EHT media three times per week.
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bioRxiv - Molecular Biology 2021Quote: ... Adherent bone marrow stromal cells were then re-plated in triplicate in 12 well plates at 2×105 cells per well with ascorbic acid and 10 mM β‐glycerophosphate (Sigma) to perform qPCR assays ...
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bioRxiv - Neuroscience 2021Quote: ... 6 ml 85% ortho-phosphoric acid preheated to 120 °C in an oil bath were added to 400 mg of (±)-epinephrine hydrochloride (E4642, Sigma-Aldrich) in a 2-dram glass scintillation vial ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... brain slices were first incubated in a 2N-HCl for 45 minutes at 45°C and then rinsed in a boric acid (Sigma-Aldrich) solution 0.1 M at pH= 8.5 for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... and the data analysis code package were as previously described.15 Na+-HEPES buffer was prepared from the free acid (Sigma-Aldrich), adjusted to pH 8 with NaOH ...
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bioRxiv - Biochemistry 2021Quote: All separations were performed isocratically at 25°C with a mobile phase comprised of 50 mM TIP solution buffered to pH 3.5 via phosphoric acid (99% Sigma-Aldrich, Australia). The flow rate was maintained at 0.4 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... Luminol (cat #A-8511) and p-coumaric acid (cat # C-9008) used to make chemiluminescence solution for western blotting were purchased from Sigma-Aldrich.
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bioRxiv - Synthetic Biology 2021Quote: ... with synthetic complete medium (ForMedium LoFlo yeast nitrogen base CYN6510 and Formedium complete supplement mixture DCS0019) except for histidine competition experiment where histidine drop-out amino-acid mixture was used (Sigma Y1751) and complemented with desired levels of histidine (Sigma 53319).
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bioRxiv - Neuroscience 2021Quote: ... Carefully plasma was discarded and acid citrate dextrose solution (0.48% citric acid, 1.32% sodium citrate, 1.47% glucose, all chemicals were purchased from Sigma Aldrich (St. Louis, MO)) was used to wash the sample by centrifuging at 2000 rpm at 4 °C for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... LECs and imLECs were starved in serum starvation medium (EBM-2 with hydrocortisone, ascorbic acid, GA-1000 all at proprietary concentrations and 2% bovine serum albumin (BSA, Sigma-Aldrich)) for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... and osteogenic induction solution (10 mmol/L β-sodium glycerol phosphate, Sigma, USA; 10−8 mol/L dexamethasone, Sigma; 50 μmmol/L ascorbic acid, Sigma; and 0.01 μmmol/L 1,25-dihydroxyvitamin D3 ...
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bioRxiv - Cell Biology 2020Quote: ... DPSCs were cultured in the mineralization-inducing fluid (5% FBS, 10 mmol/L β-sodium glycerol phosphate, Sigma; 10−7mol/L dexamethasone, Sigma; 50 ng/mL ascorbic acid, Sigma) containing 0 ...
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bioRxiv - Genomics 2021Quote: ... Degradation of the AID-tagged RAD21 was induced by the addition of 500 μM (final concentration) indole-3-acetic acid (IAA/auxin; Sigma Aldrich) during media change 24 hours prior to imaging if applicable.
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bioRxiv - Biochemistry 2021Quote: ... The electrodes were then exposed to different concentrations of amino acids (leucine, glycine, alanine, valine, isoleucine, serine, or phenylalanine, from Sigma-Aldrich) with DI washing steps before and after every exposure.
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were maintained in high amino acid medium (DMEM high glucose, 4 mM L-Glutamine, no sodium pyruvate (Sigma Aldrich), 1x MEM non-essential amino acids (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 96-well plates were fixed in 10% formalin and stained for TRAP (Acid Phosphatase Kit, Sigma-Aldrich, St. Lois, MO, USA) to visualize and count mature osteoclast numbers ...
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bioRxiv - Biophysics 2021Quote: ... hexacosanoyl Coenzyme A (C26:0-CoA, ammonium salt), Coenzyme A (CoA, trilithium salt) and Hexacosanoic acid (C26:0) were purchased from Sigma-Aldrich and solved in 5% (w/v ...
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bioRxiv - Genomics 2021Quote: ... after which it was quenched with 5 mM of ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA, Sigma, E3889). The quenched reaction was subsequently centrifuged for 5 minutes at 300 rcf and the supernatant was carefully discarded ...
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bioRxiv - Molecular Biology 2021Quote: ... S2 cells were cultured in HyClone SFX-Insect media as above and incubated in 50 nM okadaic acid (Sigma-Aldrich, O9381) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... SRP4895A), N-(p-Amylcinnamoyl)anthranilic acid (ACA, A8486), PP2 (P0042), interleukin-8 (IL-8, CXCL8 SRP3098) were purchased from Sigma-Aldrich (Sigma-Aldrich Company Ltd. ...
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bioRxiv - Microbiology 2021Quote: ... with 1.5% agar supplemented with 2 mg/mL 5-Fluoroorotic acid (US Biological, USA) and 5 µg/mL uracil (Sigma-Aldrich USA). Confirmation of plasmid excision was made by negative selection in BHIS-15% thiamphenicol plates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged and protein concentration measured using a bovine serum albumin standard curve and a bicinchoninic acid (BCA) assay (Sigma-Aldrich). The WES capillary electrophoresis system (ProteinSimple ...
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bioRxiv - Molecular Biology 2020Quote: ... The mobile phases were prepared with two solutions: (A) 10 mM ammonium formate and 0.125% formic acid (Sigma, St. Louis, MO) in ultrapure water and (B ...
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bioRxiv - Cell Biology 2021Quote: ... medium was removed from the wells and cells were washed once with PBS and fixed in prechilled 10% trichloroacetic acid (TCA) (Sigma-Aldrich) for 30 minutes at 4°C ...
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bioRxiv - Genomics 2020Quote: ... the auxin-inducible degron was induced by adding 500 μM indole-3-acetic acid (IAA, chemical analog of auxin) (Sigma, I5148) to the media ...