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Citations for Millipore Sigma :
851 - 900 of 2891 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The resulting pellet was further subjected to 33% Percoll™ (Cat. #17-0891-01, Sigma-Aldrich) gradient and centrifuged at 800g without brakes for 12 minutes at 4 degree Celsius ...
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bioRxiv - Molecular Biology 2022Quote: ... the mESCs were induced for in vitro decidualization with 10 nmol/L 17 β-estradiol (Sigma) and 1 nmol/L Progesterone (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was then carefully resuspended in 2 ml of 17% dextran (MW 60,000; Sigma, #31397) and centrifuged at 4,122g for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The nuclear fractions were obtained by using EZ-Magna Chip A/G kit (Millipore, #17-10086) and then followed by DNA shearing using Covaris (bath temperature 7.5 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 200 μL of streptavidin sepharose beads (Streptavidin Sepharose High Performance, Merck/Millipore GHC-17-5113-01) were washed thrice in 50mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: CRAF kinase activity was measured as described previously (17) and according to the manufacture’s protocol (Millipore). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of hGCase-1/17 or hGCase-1/23 or mouse IgG (Merck Millipore, #NI03) were coupled to 50 µl of Protein A magnetic Dynabeads in 200 µl Ab Binding and Washing Buffer for 30 minutes at RT on a rotating wheel ...
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bioRxiv - Microbiology 2023Quote: ... coupled to a Sigma 17-50mm F/2.8 EX DC OS (Sigma Corporation, Kawasaki, Kanagawa, Japan). The colonies were incubated for further 8 weeks to assess whether any additional morphological change would occur ...
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bioRxiv - Genetics 2024Quote: ... Cell lysis took place in 1 ml of EZ-ChIP kit lysis buffer (Millipore, 17-371RF), and chromatin was sheared into approximately 1000 bp fragments via sonication using a Diagenode Pico Bioruptor ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% Penicillin/Streptomycin (Wisent Inc #450-201-EL) additionally supplemented with 17 mM glucose (Sigma #G5767). Cells were counted using Trypan blue exclusion ...
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bioRxiv - Cancer Biology 2023Quote: ChIP assays were performed using a ChIP Assay Kit (cat. 17-259; Millipore, Temecula, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... 0.25 μL of each of the two matched primers (one forward and one reverse primer for longer amplicon or one forward and one reverse primer for shorter amplicon) (Sigma-Aldrich), 1 μL of a FAM-labeled MT-ATP 8-probe or 1 μL of a MVIC-labeled GAPDH-probe (both probes from Applied Biosystems ...
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bioRxiv - Systems Biology 2021Quote: ... Three reference genes were selected for gene expression analysis: TBP, HPRT1, and GAPDH (Allen et al, 2008).) All primers were KiCqStart primers designed by and purchased from Sigma Aldrich. Ct values and PCR amplification efficiencies were calculated using LinRegPCR software (version 2017.1 ...
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bioRxiv - Molecular Biology 2020Quote: Primer sequences for markers were obtained from the UCSC genome browser and standard HEX-tagged primers (Sigma-Aldrich, Supplementary Table 2) were used to assess the flanking haplotypes of MMP20 ...
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bioRxiv - Cell Biology 2020Quote: ... Expression of genes of interest was measured using primers designed with the NCBI Primer designing tool and custom-synthesized by Sigma-Aldrich. We list primers used in the present study (Table S9) ...
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bioRxiv - Synthetic Biology 2022Quote: ... based plasmid encoding GR1-CFP was generated in a prior study.21 Three forward primers and one reverse primer were synthesized using the 22c-trick (Sigma-Aldrich) to generate each SSM library as previously described (Table S2).21,59 E ...
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bioRxiv - Genetics 2021Quote: All ORF-specific PCR primer sequences used in this validation are shown in Additional file 1: Table S3 and primers were obtained from Millipore Sigma unless mentioned otherwise from Integrated DNA Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were washed two times with PBS and incubated for two hours at RT with corresponding secondary antibodies and subsequently incubated for 30 min at RT with DAPI (Sigma). Stained sections were washed two times with PBS and mounted with either Fluorsave (Chemicon ...
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bioRxiv - Microbiology 2020Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Physiology 2021Quote: ... For the RT-qPCR the Kappa Mix (Sigma) was used and the primers concentration was 10 µM ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilized at RT with 0.25% TritonX100 (Sigma #T9284) for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 mg Fast Blue RT (Sigma-Aldrich) in 100 ml of 50 mM 2-amino-2-methyl-1,3-propanediol buffer (pH 9.8) ...
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bioRxiv - Cell Biology 2023Quote: ... After fixation cells were placed in hybridization buffer (5% dextran sulfate, 50% formamide, 5× SSC, 100 μg/ml heparin, 100 μg/ml sonicated salmon sperm DNA (Sigma-Aldrich, cat. No. D9156),0.1% Tween-20 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then spun down at 7,000g for 8 minutes and resuspended in 100 μL secondary hybridization buffer (10% Formamide, 2X SSC, 10% Ficoll PM-400 (Sigma-Aldrich F5415-25 mL)) ...
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bioRxiv - Cancer Biology 2023Quote: ... we mounted the slides in an anti-bleach imaging buffer containing 2X SSC/10 mM Tris-HCl/10 mM Trolox (Sigma Aldrich, cat. no. 238813)/37 ng/μL glucose oxidase (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 min at 95 °C, and then mixed with 50 μL hybridization buffer H (4x SSC, 4 mg/ml acetylated BSA (Sigma Fluka Aldrich, B8894-1.5mL), 20 mM vanadyl ribonuclease complex) ...
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bioRxiv - Cancer Biology 2021Quote: RIP assays were conducted to explore the relationship between lncRNA NEAT1 and miR-1299 via the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA). Initially ...
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bioRxiv - Microbiology 2022Quote: ... and oligo(dT) primers (Eurofins Genomics) for detection of mRNA or a fluA uni12 forward primer [92] (Sigma-Aldrich, 5’-AGCAAAAGCAGG-3’) to detect vRNA according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and treated with DNAse (On-Column DNase I Digestion set, Sigma, Inc., USA). cDNA libraries were prepared with the TruSeq RNA sample Preparation v2 kit (Illumina Inc. ...
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bioRxiv - Bioengineering 2022Quote: ... Another set of cleaned coverslips was treated with poly-L-lysine (Sigma Aldrich) for 45 minutes at 37°C in a humid chamber ...
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bioRxiv - Genetics 2022Quote: ... The kidneys were set up for culture on Whatman membranes (Sigma, catalog# WHA110409) in an air-medium interface as described26 ...
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bioRxiv - Cell Biology 2022Quote: ... plus protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore), 1 mM Na3VO4 and 1mM NaF) ...
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bioRxiv - Microbiology 2020Quote: The magnetic extraction set-up consisted of three 3 mL cuvettes (Sigma Aldrich) and a neodymium magnet (60×10×3 mm ...
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bioRxiv - Systems Biology 2020Quote: ... An initial set of cultures were inoculated into LB media (Lennox, Sigma-Aldrich) containing 25 μg/mL chloramphenicol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... a specific short hairpin RNA (shRNA) construct set was obtained from Sigma-Aldrich, and lentivirus production plasmids psPAX2 and pMD2.G were obtained from Addgene ...
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bioRxiv - Genomics 2024Quote: The 1st and 2nd sets of Cell Barcodes are synthesized by Sigma-Aldrich. The 3rd set of Cell Barcodes are synthesized by IDT.
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bioRxiv - Cell Biology 2020Quote: ... RNA immunoprecipitation was conducted using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma 17-700) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Roots were transferred to chloral hydrate solution [70 % chloral hydrate (Sigma Aldrich, cat No. 302-17-0), 10 % glycerol (Sigma Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... Aqueous silk was lyophilized and then dissolved in a 17% hexafluoroisopropanol solution (Sigma-Aldrich, St. Louis, MO) overnight ...
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bioRxiv - Plant Biology 2021Quote: ... ∼10-15 seedlings were transferred to fresh solid MS containing 20µM 17-ß- estradiol or DMSO (Sigma). For heat-shock experiments ...
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bioRxiv - Cancer Biology 2022Quote: The RIP assay was performed using a Magna RIPTM RNA Binding Protein Immunoprecipitation Kit (17-700; Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The cytoskeletal fraction was separated using the ProteoExtract® Cytoskeleton Enrichment and Isolation Kit (17-10195; Millipore), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were further purified by centrifugation over a Percoll gradient (GE Healthcare, 17-0891-01, Sigma Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ChIP experiments were performed using EZ-Magna ChIPTM A/G Chromatin Immunoprecipitation Kit (Merck Millipore, 17-10086) according to the manufacturer’s procedures ...
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bioRxiv - Immunology 2020Quote: ... After 17 hours of infection cells were labelled with 1 mg/ml FITC-Dextran 40S (Sigma Aldrich) and incubated for 1 hour at 37°C and 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ChIP and ChIPmentation datasets were produced using the following commercial antibodies: 4ul anti-H3K4me3 (17-614, Millipore), 4ul anti-H3K27me3 (17-622 ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation was performed using manufacturer’s instructions for the EZ-Nuclear RIP Kit (EMD Millipore, #17-10 523), followed by cDNA synthesis and qRT-PCR for 5’ETS ...
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bioRxiv - Genomics 2020Quote: ... followed by addition of 5 μL of H3K4me3 (histone H3 lysine 4 trimethylation) antibody (Millipore, 17-614) and incubation overnight at 4°C with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... primary antibodies were applied: Anti-O-GlcNAc Transferase (DM-17, Sigma-Aldrich, catalogue number O6264, 1:5000), RL2 (Novus ...
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bioRxiv - Microbiology 2023Quote: RNA-binding protein immunoprecipitation (RIP) was conducted using the Magna RIP kit (catalog no. 17-700; Millipore) following the manufacturer’s instructions ...