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Citations for Millipore Sigma :
651 - 700 of 2891 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Eighteen microliters of high-titer lentivirus expressing miR-382 mixed with 2 μL 0.2% Evans blue dye (Sigma-Aldrich) in PBS was injected in the third mammary gland using a 34-gauge needle (Hamilton) ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos are rinsed in HB-buffer (HB+ without torula RNA and heparin) supplemented with saline-sodium citrate buffer (SSC, Sigma, S6639) and then transferred gradually to PBT ...
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bioRxiv - Genomics 2020Quote: ... The complete reaction mix was again removed and a slide wash with one 10 minute wash of the wells with 2X SSC/0.1% SDS (Sigma-Aldrich, USA), followed by 1 minute wash with 0.2X SSC and finally 0.1X SSC was performed ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS was incubated in each well for 5 minutes and cells were washed with 2x SSCT (2x SSC with 0.1% TWEEN® 20 (P9416-100ML; Sigma-Aldrich). For storage ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was then neutralized with SSC and hybridized with a DIG-labeled telomere probe prepared as described 48 in DIG Easy Hyb (Sigma-Aldrich) solution overnight at 42°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the preparations were thermally aged overnight at 37 °C and then 60 min at 60 °C, followed by treatments with RNase A (200 µg/mL in 2× SSC, 60–90 min, 37 °C) (Sigma-Aldrich) and then pepsin (50 µg/mL in 10 mM HCl ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were washed with wash buffer (10% formamide in 1X SSC and 0.1% Tween-20) for 5 min, then incubated with the hybridization mix (10% formamide, 1X SSC, 10% Dextran sulfate (w/v) (Sigma, D8906), 100nM each in situ probe ...
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bioRxiv - Genomics 2022Quote: ... before slides were detached from their hybridization chambers and washed for 10min at 50°C in 2X SSC/0.1% SDS (Sigma-Aldrich, USA), followed by 1 minute wash with 0.2X SSC and finally 0.1X SSC ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently washed twice with PBS and once with prehybridization buffer (10% deionized formamide [Millipore] in 2x concentrated SSC [Gibco]) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then pre-hybridized for 20 minutes at 37°C in wash buffer (2x SSC, 10% formamide (Sigma-Aldrich, F9037)) ...
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bioRxiv - Immunology 2023Quote: ... the gel was rinsed again with water and soaked in 10X SSC for 30-60 min before capillary transfer to an ImmobilonP membrane (Millipore Sigma), as described previously120 ...
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bioRxiv - Systems Biology 2023Quote: The coverslip was equilibrated in 1 ml of 30% formamide buffer composed of 2×SSC and 30% deionised formamide (Sigma, F9037) in a 12-well plate for >5 min prior to hybridisation ...
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bioRxiv - Systems Biology 2023Quote: ... the disulfide bond linking the probe and the fluorophore was cleaved with Cleavage buffer comprising 2×SSC and 50 mM TCEP (Sigma, 646547) for 15 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 nM readout probes in 10% EC Buffer (1 mL of ethylene carbonate, 7 mL Ultrapure RNase/DNase free water, 2 mL 20x SSC, and 1g Low MW dextran sulfate Sigma #D4911) was added to the cells and incubated for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... An anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2×SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% TritonX100 in 2 × SSC), anti-bleaching buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 3 mM Trolox (Sigma 238813), 0.8% D-glucose (Nacalai Tesque 168060-25) ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated in 20x SSC at 65 °C and stained for 20 min with 5% Giemsa solution (Sigma-Aldrich, GS500).
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bioRxiv - Evolutionary Biology 2020Quote: ... All primers were obtained from Sigma-Aldrich Ltd ...
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bioRxiv - Developmental Biology 2020Quote: ... purchased from Sigma-Aldrich (KiCqStart SYBR Green Primers Predesigned, Sigma Aldrich) are listed in Table S1.
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bioRxiv - Neuroscience 2021Quote: ... and the following primers (Sigma, 5′-3′): 18S F ...
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bioRxiv - Molecular Biology 2020Quote: ... and mRNA sequence specific primers (Sigma-Aldrich). The qPCR was performed with 2 μL of a 1:10 dilution of the cDNA for the genes ACT1 ...
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bioRxiv - Immunology 2022Quote: ... 0.2 mM of reverse primer (Sigma Aldrich) and nuclease-free water to a total volume of 10 μL (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... 0.2 mM of forward primer (Sigma Aldrich), 0.2 mM of reverse primer (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... All primers used were ordered from Sigma and all Sanger sequencing reactions were done at Stabvida.
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bioRxiv - Physiology 2020Quote: ... All primers were synthesised by Sigma Aldrich. Primer sequences ...
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bioRxiv - Physiology 2020Quote: ... All primers were purchased from Sigma-Aldrich.
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bioRxiv - Immunology 2020Quote: ... The forward and reverse phosphorylated primers (Sigma Chemical ...
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bioRxiv - Cancer Biology 2019Quote: ... The mRNA primer designs (Sigma-Aldrich, USA) are listed in the Supplementary Table 3a ...
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bioRxiv - Genomics 2021Quote: ... 1 µL i7 Index primer (Sigma-Aldrich), and 1 µL i5 Index primer (IDT ...
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bioRxiv - Plant Biology 2021Quote: ... with primers (Sigma Aldrich, St. Louis, US) listed in Table ...
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bioRxiv - Neuroscience 2020Quote: ... 1.25 μM primer pair (Sigma, Dublin, Ireland) and RNAse free water (Invitrogen CA ...
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bioRxiv - Biochemistry 2021Quote: ... Oligonucleotide primers were synthesized by Sigma-Aldrich. Genes from the bbu gene cluster were amplified from 10 ng E ...
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bioRxiv - Biochemistry 2020Quote: ... All primers were synthesized by Sigma Aldrich. For DNA purification ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR primers were purchased from Sigma-Aldrich, KiCqStart Primers ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.4 ul of reverse primer (Sigma Aldrich), 5.0 ul of Kapa Syber Fast Low Rox (Sigma Aldrich KK4622) ...
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bioRxiv - Immunology 2022Quote: ... The sequences of primer pairs (Sigma-Aldrich) specific for murine Gapdh (Forward ...
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bioRxiv - Immunology 2022Quote: ... qPCR primers were purchased from Sigma-Aldrich. A complete list of primer sequences is provided in Supplementary Table 1.
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bioRxiv - Genetics 2019Quote: ... Primers for SYBR green qPCR (Sigma-Aldrich) were designed using Primer-BLAST (NCBI ...
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bioRxiv - Zoology 2020Quote: ... 200 μM sense and antisense primers (Sigma), 0.625 U Taq DNA Polymerase (GeneDireX) ...
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bioRxiv - Zoology 2020Quote: ... 200 μM sense and antisense primers (Sigma), 0.625 U Taq DNA polymerase and cDNA template ...
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bioRxiv - Synthetic Biology 2020Quote: ... All primers were obtained from Sigma-Aldrich.
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bioRxiv - Immunology 2019Quote: ... and 10μM primers (Sigma-Aldrich, MO, USA). Expression of microcephalin-1 (MCPH1) ...
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bioRxiv - Cell Biology 2019Quote: ... All primers were synthesized by Sigma-Aldrich and purified by HPLC ...
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bioRxiv - Cancer Biology 2021Quote: ... The primers were purchased from Sigma-Aldrich and diluted to 10 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... The primers were purchased from Sigma-Aldrich and diluted to 10 μM.
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bioRxiv - Cell Biology 2020Quote: ... qPCR was performed with primer pairs (Sigma) listed in Table S1 ...
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bioRxiv - Neuroscience 2021Quote: ... All primers were obtained from Sigma-Aldrich and described in Suppl ...
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bioRxiv - Microbiology 2021Quote: ... using PAGE-purified oligonucleotide primers (Sigma-Aldrich). Diagnostic PCR was performed with DreamTaq PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... All primers were purchased from Sigma-Aldrich (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... All primers were purchased from Sigma (UK).