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Citations for Millipore Sigma :
801 - 850 of 2891 citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Primers and DNAzymes were purchased from Sigma Aldrich. Purified S ...
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bioRxiv - Molecular Biology 2021Quote: ... and DuetDOWN1 primers (Novagen Cat. No. 71179-3), as well as the original primers ...
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bioRxiv - Cell Biology 2020Quote: ... E@sy Oligo primers (Millipore-Sigma, Burlington, MA) or TaqMan Assays (Life Technology ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μl of each 10 μM primer (Sigma), 2μl of 10X PCR buffer (Qiagen 203203) ...
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bioRxiv - Immunology 2021Quote: ... 0.5 μg primer for cDNA synthesis (Sigma Aldrich) and RNasin inhibitor (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... long primers (90 bp in total, Sigma-Aldrich) were used ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Oligonucleotides (DNA primers) were obtained from Sigma Aldrich unless specified otherwise and are mentioned in Supplementary Table 1.
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bioRxiv - Molecular Biology 2023Quote: The desalted primers were synthesized by Sigma-Aldrich chemicals Pvt ...
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bioRxiv - Cell Biology 2023Quote: ... All the primers were synthesized from Sigma-Aldrich. Barcode Biosciences ...
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bioRxiv - Immunology 2023Quote: We obtained the AMP primers from Sigma-Aldrich Ltd ...
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bioRxiv - Genomics 2023Quote: ... Primers were diluted in Nuclease-free water (Sigma) to use in a final concentration of 10 µM.
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bioRxiv - Microbiology 2023Quote: ... primers listed in Table S3 (Sigma, Bangalore, India) were designed to amplify the kanamycin cassette from the template ...
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bioRxiv - Cell Biology 2022Quote: ... with 150nM Forward and Reverse primers (Sigma-Aldrich; Supplementary Table 5 for primer sequences) ...
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bioRxiv - Cell Biology 2022Quote: ... Primers used for SYBR Green assays (Sigma-Aldrich) can be found in Table S4.
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bioRxiv - Neuroscience 2023Quote: ... 0.5 μl of each primer (25 μM; Sigma), and 4 μl of 1:20 diluted lysed tail DNA in a final volume of 25 µl using dH2O ...
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bioRxiv - Microbiology 2023Quote: ... and the Uni12 primer (5’-AGCAAAAGCAGG-3’, Sigma). For mRNA analysis Oligo(dT ...
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bioRxiv - Immunology 2024Quote: ... 1 µL i7 Index primer (Sigma-Aldrich®), and 1 µL i5 Index primer (IDT ...
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bioRxiv - Microbiology 2024Quote: ... Using gene-specific primers purchased from Sigma-Aldrich, the PCR experiment was carried out employing the temperature profile shown in Table S4 ...
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bioRxiv - Physiology 2024Quote: ... The primers were all purchased from Millipore Sigma with sequences listed as below ...
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bioRxiv - Neuroscience 2024Quote: ... and the following gene-specific primers (Sigma-Aldrich): csf1 (NM_007778.4 ...
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bioRxiv - Microbiology 2024Quote: ... antibiotics and primers were purchased from Sigma-Aldrich. Restriction and cloning enzymes were obtained from New England Biolabs (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA for reverse transcription polymerase chain reaction (RT-PCR) was isolated from 100 mg of oat leaves using TRI REAGENT® (Sigma-Aldrich, St. Louis, MO, USA) according to the provided protocol ...
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bioRxiv - Physiology 2020Quote: ... miR-150fl/Cdh5(PAC)-iCreERT2 mice were injected intraperitoneally with 100 µL of 5 mg/mL tamoxifen (Sigma, cat. no. T5648) or vehicle (12.5% vol/vol ethanol in peanut oil ...
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bioRxiv - Cancer Biology 2019Quote: ... mice were treated via tail vein injection with 80 μg of control miRNA or 5-FdU-miR-15a packaged using in vivo-jetPEI (Polyplus Transfection, Illkirch, France) or with 50 mg/kg of gemcitabine (Sigma Aldrich) via intraperitoneal injection (IP injection) ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Genomics 2024Quote: ... samples were covered with a digestion solution (1% proteinase K, 20% v/v SDS [ThermoFisher, AM9823], 0.25% v/v triton-X [Sigma, T8787] in 2X SSC) and incubated at 37 °C for 24 hours ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM βME and protease inhibitor (Protease Inhibitor Cocktail Set III, Sigma)) and sonicated on ice for 1 minute 30 seconds (15s ON ...
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bioRxiv - Cell Biology 2021Quote: ... PLA was performed using Duolink in situ Starter Set GREEN (Sigma-Aldrich) according to manufacturer’s instructions using rabbit anti-Myc polyclonal antibody and mouse anti-Flag monoclonal antibody.
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bioRxiv - Neuroscience 2020Quote: ... nAChRα5 and nAChRα6 DNA oligonucleotide sets were synthesised by Sigma-Aldrich (Merck) and enzymatically labelled with ATTO-633 according to (Gaspar et al. ...
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bioRxiv - Neuroscience 2019Quote: ... In the set of experiments involving rapamycin (200 nM, Sigma-Aldrich, Germany) slices were let to recover in rapamycin-infused aCSF or DMSO (control group ...
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bioRxiv - Biochemistry 2022Quote: ... 10 mM βME and protease inhibitor (Protease Inhibitor Cocktail Set III, Sigma)) and sonicated on ice for 1 minute 30 seconds (15s ON ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 1:200 Protease Inhibitor Cocktail Set III (#539134, Merck Millipore)) ...
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bioRxiv - Biophysics 2019Quote: ... set on Millicell Cell Culture Inserts (Millipore, 0.4 mm; 30 mm diameter), and cultured for up to 14 days on multiwell-plates at 35°C/5% CO2 in a culture medium composed of 50% Basal Medium Eagle ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sets were stored either in PBS with 0.1% sodium azide (Sigma) at 4°C or in cryoprotectant comprised of glycerol ...
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bioRxiv - Physiology 2022Quote: ... tissues were homogenized using a KIMBLE Dounce tissue grinder set (Sigma Aldrich, catalog no.
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bioRxiv - Neuroscience 2023Quote: ... and 0.5% (v/v) Phosphatase Inhibitor Cocktail Set V (Millipore Sigma, 524629). The resulting lysates were centrifuged to collect supernatant for downstream Western Blotting ...
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bioRxiv - Physiology 2024Quote: ... and phosphatase inhibitor cocktail set II (Millipore, 524625-1set, diluted 1:100) using an automatic homogeniser (VWR ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were transduced with a LentiBrite™ paxillin-GFP lentiviral biosensor (Millipore Sigma, Catalog #17-10154) at a MOI of 20 in 1 ml of growth media per well ...
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bioRxiv - Microbiology 2019Quote: ... Both 293T/17 and TZM-bl cells were maintained in Dulbecco’s modified Eagle Medium (DMEM; Sigma) containing 10% cosmic calf serum (CCS ...
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bioRxiv - Cell Biology 2020Quote: ... After two days cells were harvested and lysed using the MAGNA-RIP kit (Millipore 17-700) according to the manufactureŕs protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... were transduced to stably express cytoplasmic fluorescence (LentiBriteTM GFP Control Lentiviral Biosensor 17-10387, Millipore Sigma) and cultured in Vasculife Endothelial Medium (LL-0003 ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ChIP was performed following the protocol for EZ ChIP™ Chromatin Immunoprecipitation kit (Millipore, 17-371) with the following modifications ...
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bioRxiv - Cell Biology 2021Quote: ... the water was replaced with 100 μl of reaction mix (17 μg/ml of Luminol (Sigma), 10 μg/ml of Horseradish Peroxidase (HRP ...
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bioRxiv - Cancer Biology 2019Quote: ... was mixed with collagen and neutralising agent (N.A.) (17% 10 M NaOH (Sigma-Aldrich, Dorset, UK) in 1 M HEPES buffer Gibco™through Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: RNA-binding protein immunoprecipitation was run according to supplier recommendations (Millipore, Magna RIP Kit, 17-700). Beads were conjugated with 10 μg either anti-IMP3 (#1 ...
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bioRxiv - Developmental Biology 2020Quote: ... some of the organoids were injected with LentiBrite™ RFP Control Lentiviral Biosensor (Millipore, 17-10409), using the same procedure as with EmGFP Sendai virus ...
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bioRxiv - Developmental Biology 2020Quote: RNA immunoprecipitation was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (17-700; Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: RNA-binding protein immunoprecipitation (RIP) was performed using a Magna RIP kit (Cat# 17-700, Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2021Quote: ... the water was replaced with 100 μl of reaction mix (17 μg/ml of Luminol (Sigma), 10 μg/ml of Horseradish Peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy5-conjugated secondary antibodies (1:200; AP192SA6; Merck Millipore, USA; 17 hrs at 4°C). Similar immunolabeling steps were followed for the subsequent sequential staining ...